Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues
The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular...
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Reichardt, Marius [verfasserIn] |
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E-Book |
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Englisch |
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Erscheinungsort nicht ermittelbar: Universitätsverlag Göttingen ; 2022 |
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Open Access Creative Commons ; by-sa/4.0/deed.de |
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1 Online-Ressource |
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| 520 | |a The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed | ||
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(DE-627)1836516436 (DE-599)KEP077644352 (OCoLC)1366209274 (OAPEN)53509 (EBP)077644352 DE-627 eng DE-627 rda eng PH bicssc Reichardt, Marius verfasserin aut Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues [Erscheinungsort nicht ermittelbar] Universitätsverlag Göttingen 2022 1 Online-Ressource Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Open Access Unrestricted online access star The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed Creative Commons by-sa/4.0/deed.de cc English Physics https://library.oapen.org/bitstream/id/e7ac6adf-83c9-4080-92f0-fcea7ba3efa4/GSXP28_reichardt.pdf X:OAPEN Verlag kostenfrei https://library.oapen.org/handle/20.500.12657/53509 X:OAPEN Verlag kostenfrei ZDB-94-OAL GBV_ILN_22 ISIL_DE-18 SYSFLAG_1 GBV_KXP GBV_ILN_23 ISIL_DE-830 GBV_ILN_30 ISIL_DE-104 GBV_ILN_31 ISIL_DE-27 GBV_ILN_34 ISIL_DE-18-302 GBV_ILN_39 ISIL_DE-547 GBV_ILN_40 ISIL_DE-7 GBV_ILN_63 ISIL_DE-Wim2 GBV_ILN_65 ISIL_DE-3 GBV_ILN_70 ISIL_DE-89 GBV_ILN_72 ISIL_DE-35 GBV_ILN_95 ISIL_DE-542 GBV_ILN_110 ISIL_DE-Luen4 GBV_ILN_136 ISIL_DE-Wis1 GBV_ILN_147 ISIL_DE-Fl3 GBV_ILN_161 ISIL_DE-960 GBV_ILN_187 ISIL_DE-Ki95 GBV_ILN_213 ISIL_DE-551 GBV_ILN_230 ISIL_DE-552 GBV_ILN_283 ISIL_DE-Ha163 GBV_ILN_293 ISIL_DE-960-3 GBV_ILN_370 ISIL_DE-1373 GBV_ILN_603 ISIL_DE-B1556 GBV_ILN_808 GBV_ILN_2403 ISIL_DE-LFER BO 22 01 0018 4280172285 OLR-ZDB-94-OAL OAPEN Library zu 01-03-23 23 01 0830 4280724628 OLR-OAPEN f z 01-03-23 30 01 0104 4281085718 GBV-OAPEN z 01-03-23 31 01 0027 4280367051 z 01-03-23 34 01 3551 4278922566 OLR-OAPEN zi002 01-03-23 39 01 0547 4281277137 GBV-OAPEN ke 01-03-23 40 01 0007 4279991162 OLR-OAPEN xsn 01-03-23 63 01 3401 4281660763 E-Books LF GBV-OAPEN z 01-03-23 65 01 0003 4281910115 OLR-OAPEN z 01-03-23 70 01 0089 4280545537 OLR-OAPEN-OA OAPEN Online Library Open Access eBook z 01-03-23 72 01 0035 4278745222 OLR-OAPEN-OA OAPEN Online Library Open Access eBook z 01-03-23 95 01 3095 4343272877 OLR-OAL Vervielfältigungen (z.B. 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(DE-627)1836516436 (DE-599)KEP077644352 (OCoLC)1366209274 (OAPEN)53509 (EBP)077644352 DE-627 eng DE-627 rda eng PH bicssc Reichardt, Marius verfasserin aut Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues [Erscheinungsort nicht ermittelbar] Universitätsverlag Göttingen 2022 1 Online-Ressource Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Open Access Unrestricted online access star The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed Creative Commons by-sa/4.0/deed.de cc English Physics https://library.oapen.org/bitstream/id/e7ac6adf-83c9-4080-92f0-fcea7ba3efa4/GSXP28_reichardt.pdf X:OAPEN Verlag kostenfrei https://library.oapen.org/handle/20.500.12657/53509 X:OAPEN Verlag kostenfrei ZDB-94-OAL GBV_ILN_22 ISIL_DE-18 SYSFLAG_1 GBV_KXP GBV_ILN_23 ISIL_DE-830 GBV_ILN_30 ISIL_DE-104 GBV_ILN_31 ISIL_DE-27 GBV_ILN_34 ISIL_DE-18-302 GBV_ILN_39 ISIL_DE-547 GBV_ILN_40 ISIL_DE-7 GBV_ILN_63 ISIL_DE-Wim2 GBV_ILN_65 ISIL_DE-3 GBV_ILN_70 ISIL_DE-89 GBV_ILN_72 ISIL_DE-35 GBV_ILN_95 ISIL_DE-542 GBV_ILN_110 ISIL_DE-Luen4 GBV_ILN_136 ISIL_DE-Wis1 GBV_ILN_147 ISIL_DE-Fl3 GBV_ILN_161 ISIL_DE-960 GBV_ILN_187 ISIL_DE-Ki95 GBV_ILN_213 ISIL_DE-551 GBV_ILN_230 ISIL_DE-552 GBV_ILN_283 ISIL_DE-Ha163 GBV_ILN_293 ISIL_DE-960-3 GBV_ILN_370 ISIL_DE-1373 GBV_ILN_603 ISIL_DE-B1556 GBV_ILN_808 GBV_ILN_2403 ISIL_DE-LFER BO 22 01 0018 4280172285 OLR-ZDB-94-OAL OAPEN Library zu 01-03-23 23 01 0830 4280724628 OLR-OAPEN f z 01-03-23 30 01 0104 4281085718 GBV-OAPEN z 01-03-23 31 01 0027 4280367051 z 01-03-23 34 01 3551 4278922566 OLR-OAPEN zi002 01-03-23 39 01 0547 4281277137 GBV-OAPEN ke 01-03-23 40 01 0007 4279991162 OLR-OAPEN xsn 01-03-23 63 01 3401 4281660763 E-Books LF GBV-OAPEN z 01-03-23 65 01 0003 4281910115 OLR-OAPEN z 01-03-23 70 01 0089 4280545537 OLR-OAPEN-OA OAPEN Online Library Open Access eBook z 01-03-23 72 01 0035 4278745222 OLR-OAPEN-OA OAPEN Online Library Open Access eBook z 01-03-23 95 01 3095 4343272877 OLR-OAL Vervielfältigungen (z.B. Kopien, Downloads) sind nur von einzelnen Kapiteln oder Seiten und nur zum eigenen wissenschaftlichen Gebrauch erlaubt. Die Weitergabe an Dritte sowie systematisches Downloaden sind untersagt. z 24-06-23 110 01 3110 4281462937 OLR-GBV-OAPEN Open Access z 01-03-23 136 01 3526 4279636532 OLR-OAPEN z 01-03-23 147 01 3528 4282108630 OLR-OAPEN frei verfügbar für Europa-Universität Flensburg, Hochschule Flensburg und Zentrale Hochschulbibliothek Vervielfältigungen (z.B. Kopien, Downloads) sind nur von einzelnen Kapiteln oder Seiten und nur zum eigenen wissenschaftlichen Gebrauch erlaubt. Die Weitergabe an Dritte sowie systematisches Downloaden sind untersagt. z 01-03-23 161 01 0960 4278179243 OLR-OAPEN OAPEN Online Library Open Access eBook z 28-02-23 187 01 3520 4278563728 JSTOR Open Access eBook z 28-02-23 213 01 0551 4279277737 OLR-OAPEN OAPEN Online Library Open Access eBook z 01-03-23 230 01 0552 4279454914 OLR-OAPEN OAPEN Online Library Open Access eBook z 01-03-23 283 01 3283 4279813825 OLR-OAL z 01-03-23 293 01 3293 4278358083 OLR-OAPEN OAPEN Online Library Open Access eBook z 28-02-23 370 01 4370 4279100306 OLR-FREE Kostenloser Zugriff z 01-03-23 603 01 4603 4278001029 OLR-OAPEN Bitte beachten Sie die Nutzungsbedingungen und Copyright-Bestimmungen des Verlages/Herausgebers. z 28-02-23 808 01 4808 4280903743 OLR-OAPEN OAPEN Library zg 01-03-23 2403 01 DE-LFER 4308614228 00 --%%-- --%%-- n --%%-- l01 13-04-23 22 01 0018 Volltextzugang https://library.oapen.org/handle/20.500.12657/53509 23 01 0830 https://library.oapen.org/handle/20.500.12657/53509 30 01 0104 Open Access https://library.oapen.org/handle/20.500.12657/53509 31 01 0027 eBook GBV-OAPEN https://library.oapen.org/handle/20.500.12657/53509 34 01 3551 OpenAccess https://library.oapen.org/handle/20.500.12657/53509 40 01 0007 Volltext, Open Access https://library.oapen.org/handle/20.500.12657/53509 63 01 3401 E-Book https://library.oapen.org/handle/20.500.12657/53509 65 01 0003 Open Access https://library.oapen.org/handle/20.500.12657/53509 65 01 0003 Dieser Titel ist Teil einer Datenbank http://www.bibliothek.uni-regensburg.de/dbinfo/detail.php?titel_id=10728&bib_id=ulb_hal 70 01 0089 https://library.oapen.org/handle/20.500.12657/53509 LF 72 01 0035 https://library.oapen.org/handle/20.500.12657/53509 95 01 3095 https://library.oapen.org/handle/20.500.12657/53509 110 01 3110 Open Access https://library.oapen.org/handle/20.500.12657/53509 136 01 3526 Open Access https://library.oapen.org/handle/20.500.12657/53509 147 01 3528 https://library.oapen.org/handle/20.500.12657/53509 161 01 0960 https://library.oapen.org/handle/20.500.12657/53509 LF 187 01 3520 https://library.oapen.org/handle/20.500.12657/53509 213 01 0551 https://library.oapen.org/handle/20.500.12657/53509 230 01 0552 https://library.oapen.org/handle/20.500.12657/53509 283 01 3283 https://library.oapen.org/handle/20.500.12657/53509 293 01 3293 https://library.oapen.org/handle/20.500.12657/53509 LF 370 01 4370 https://library.oapen.org/handle/20.500.12657/53509 603 01 4603 https://library.oapen.org/handle/20.500.12657/53509 808 01 4808 Volltextzugang https://library.oapen.org/handle/20.500.12657/53509 2403 01 DE-LFER https://library.oapen.org/bitstream/id/e7ac6adf-83c9-4080-92f0-fcea7ba3efa4/GSXP28_reichardt.pdf 31 01 0027 00 eBook GBV-OAPEN 22 01 0018 OLR-ZDB-94-OAL 23 01 0830 OLR-OAPEN 30 01 0104 GBV-OAPEN 34 01 3551 OLR-OAPEN 39 01 0547 GBV-OAPEN 39 01 0547 LF 40 01 0007 OLR-OAPEN 63 01 3401 E-Books LF GBV-OAPEN 65 01 0003 OLR-OAPEN 70 01 0089 OLR-OAPEN-OA 72 01 0035 OLR-OAPEN-OA 95 01 3095 OLR-OAL 110 01 3110 OLR-GBV-OAPEN 136 01 3526 OLR-OAPEN 147 01 3528 OLR-OAPEN 161 01 0960 OLR-OAPEN 213 01 0551 OLR-OAPEN 230 01 0552 OLR-OAPEN 283 01 3283 OLR-OAL 293 01 3293 OLR-OAPEN 370 01 4370 OLR-FREE 370 01 4370 olr-ebook GBV-OAPEN 603 01 4603 OLR-OAPEN 808 01 4808 OLR-OAPEN |
| allfields_unstemmed |
(DE-627)1836516436 (DE-599)KEP077644352 (OCoLC)1366209274 (OAPEN)53509 (EBP)077644352 DE-627 eng DE-627 rda eng PH bicssc Reichardt, Marius verfasserin aut Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues [Erscheinungsort nicht ermittelbar] Universitätsverlag Göttingen 2022 1 Online-Ressource Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Open Access Unrestricted online access star The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed Creative Commons by-sa/4.0/deed.de cc English Physics https://library.oapen.org/bitstream/id/e7ac6adf-83c9-4080-92f0-fcea7ba3efa4/GSXP28_reichardt.pdf X:OAPEN Verlag kostenfrei https://library.oapen.org/handle/20.500.12657/53509 X:OAPEN Verlag kostenfrei ZDB-94-OAL GBV_ILN_22 ISIL_DE-18 SYSFLAG_1 GBV_KXP GBV_ILN_23 ISIL_DE-830 GBV_ILN_30 ISIL_DE-104 GBV_ILN_31 ISIL_DE-27 GBV_ILN_34 ISIL_DE-18-302 GBV_ILN_39 ISIL_DE-547 GBV_ILN_40 ISIL_DE-7 GBV_ILN_63 ISIL_DE-Wim2 GBV_ILN_65 ISIL_DE-3 GBV_ILN_70 ISIL_DE-89 GBV_ILN_72 ISIL_DE-35 GBV_ILN_95 ISIL_DE-542 GBV_ILN_110 ISIL_DE-Luen4 GBV_ILN_136 ISIL_DE-Wis1 GBV_ILN_147 ISIL_DE-Fl3 GBV_ILN_161 ISIL_DE-960 GBV_ILN_187 ISIL_DE-Ki95 GBV_ILN_213 ISIL_DE-551 GBV_ILN_230 ISIL_DE-552 GBV_ILN_283 ISIL_DE-Ha163 GBV_ILN_293 ISIL_DE-960-3 GBV_ILN_370 ISIL_DE-1373 GBV_ILN_603 ISIL_DE-B1556 GBV_ILN_808 GBV_ILN_2403 ISIL_DE-LFER BO 22 01 0018 4280172285 OLR-ZDB-94-OAL OAPEN Library zu 01-03-23 23 01 0830 4280724628 OLR-OAPEN f z 01-03-23 30 01 0104 4281085718 GBV-OAPEN z 01-03-23 31 01 0027 4280367051 z 01-03-23 34 01 3551 4278922566 OLR-OAPEN zi002 01-03-23 39 01 0547 4281277137 GBV-OAPEN ke 01-03-23 40 01 0007 4279991162 OLR-OAPEN xsn 01-03-23 63 01 3401 4281660763 E-Books LF GBV-OAPEN z 01-03-23 65 01 0003 4281910115 OLR-OAPEN z 01-03-23 70 01 0089 4280545537 OLR-OAPEN-OA OAPEN Online Library Open Access eBook z 01-03-23 72 01 0035 4278745222 OLR-OAPEN-OA OAPEN Online Library Open Access eBook z 01-03-23 95 01 3095 4343272877 OLR-OAL Vervielfältigungen (z.B. Kopien, Downloads) sind nur von einzelnen Kapiteln oder Seiten und nur zum eigenen wissenschaftlichen Gebrauch erlaubt. Die Weitergabe an Dritte sowie systematisches Downloaden sind untersagt. z 24-06-23 110 01 3110 4281462937 OLR-GBV-OAPEN Open Access z 01-03-23 136 01 3526 4279636532 OLR-OAPEN z 01-03-23 147 01 3528 4282108630 OLR-OAPEN frei verfügbar für Europa-Universität Flensburg, Hochschule Flensburg und Zentrale Hochschulbibliothek Vervielfältigungen (z.B. Kopien, Downloads) sind nur von einzelnen Kapiteln oder Seiten und nur zum eigenen wissenschaftlichen Gebrauch erlaubt. Die Weitergabe an Dritte sowie systematisches Downloaden sind untersagt. z 01-03-23 161 01 0960 4278179243 OLR-OAPEN OAPEN Online Library Open Access eBook z 28-02-23 187 01 3520 4278563728 JSTOR Open Access eBook z 28-02-23 213 01 0551 4279277737 OLR-OAPEN OAPEN Online Library Open Access eBook z 01-03-23 230 01 0552 4279454914 OLR-OAPEN OAPEN Online Library Open Access eBook z 01-03-23 283 01 3283 4279813825 OLR-OAL z 01-03-23 293 01 3293 4278358083 OLR-OAPEN OAPEN Online Library Open Access eBook z 28-02-23 370 01 4370 4279100306 OLR-FREE Kostenloser Zugriff z 01-03-23 603 01 4603 4278001029 OLR-OAPEN Bitte beachten Sie die Nutzungsbedingungen und Copyright-Bestimmungen des Verlages/Herausgebers. z 28-02-23 808 01 4808 4280903743 OLR-OAPEN OAPEN Library zg 01-03-23 2403 01 DE-LFER 4308614228 00 --%%-- --%%-- n --%%-- l01 13-04-23 22 01 0018 Volltextzugang https://library.oapen.org/handle/20.500.12657/53509 23 01 0830 https://library.oapen.org/handle/20.500.12657/53509 30 01 0104 Open Access https://library.oapen.org/handle/20.500.12657/53509 31 01 0027 eBook GBV-OAPEN https://library.oapen.org/handle/20.500.12657/53509 34 01 3551 OpenAccess https://library.oapen.org/handle/20.500.12657/53509 40 01 0007 Volltext, Open Access https://library.oapen.org/handle/20.500.12657/53509 63 01 3401 E-Book https://library.oapen.org/handle/20.500.12657/53509 65 01 0003 Open Access https://library.oapen.org/handle/20.500.12657/53509 65 01 0003 Dieser Titel ist Teil einer Datenbank http://www.bibliothek.uni-regensburg.de/dbinfo/detail.php?titel_id=10728&bib_id=ulb_hal 70 01 0089 https://library.oapen.org/handle/20.500.12657/53509 LF 72 01 0035 https://library.oapen.org/handle/20.500.12657/53509 95 01 3095 https://library.oapen.org/handle/20.500.12657/53509 110 01 3110 Open Access https://library.oapen.org/handle/20.500.12657/53509 136 01 3526 Open Access https://library.oapen.org/handle/20.500.12657/53509 147 01 3528 https://library.oapen.org/handle/20.500.12657/53509 161 01 0960 https://library.oapen.org/handle/20.500.12657/53509 LF 187 01 3520 https://library.oapen.org/handle/20.500.12657/53509 213 01 0551 https://library.oapen.org/handle/20.500.12657/53509 230 01 0552 https://library.oapen.org/handle/20.500.12657/53509 283 01 3283 https://library.oapen.org/handle/20.500.12657/53509 293 01 3293 https://library.oapen.org/handle/20.500.12657/53509 LF 370 01 4370 https://library.oapen.org/handle/20.500.12657/53509 603 01 4603 https://library.oapen.org/handle/20.500.12657/53509 808 01 4808 Volltextzugang https://library.oapen.org/handle/20.500.12657/53509 2403 01 DE-LFER https://library.oapen.org/bitstream/id/e7ac6adf-83c9-4080-92f0-fcea7ba3efa4/GSXP28_reichardt.pdf 31 01 0027 00 eBook GBV-OAPEN 22 01 0018 OLR-ZDB-94-OAL 23 01 0830 OLR-OAPEN 30 01 0104 GBV-OAPEN 34 01 3551 OLR-OAPEN 39 01 0547 GBV-OAPEN 39 01 0547 LF 40 01 0007 OLR-OAPEN 63 01 3401 E-Books LF GBV-OAPEN 65 01 0003 OLR-OAPEN 70 01 0089 OLR-OAPEN-OA 72 01 0035 OLR-OAPEN-OA 95 01 3095 OLR-OAL 110 01 3110 OLR-GBV-OAPEN 136 01 3526 OLR-OAPEN 147 01 3528 OLR-OAPEN 161 01 0960 OLR-OAPEN 213 01 0551 OLR-OAPEN 230 01 0552 OLR-OAPEN 283 01 3283 OLR-OAL 293 01 3293 OLR-OAPEN 370 01 4370 OLR-FREE 370 01 4370 olr-ebook GBV-OAPEN 603 01 4603 OLR-OAPEN 808 01 4808 OLR-OAPEN |
| allfieldsGer |
(DE-627)1836516436 (DE-599)KEP077644352 (OCoLC)1366209274 (OAPEN)53509 (EBP)077644352 DE-627 eng DE-627 rda eng PH bicssc Reichardt, Marius verfasserin aut Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues [Erscheinungsort nicht ermittelbar] Universitätsverlag Göttingen 2022 1 Online-Ressource Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Open Access Unrestricted online access star The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. 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The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed |
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The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed |
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