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Genome-Wide Identification and Expression Analysis of <i<TPS</i< Gene Family in <i<Liriodendron chinense</i<
Terpenoids play a key role in plant growth and development, supporting resistance regulation and terpene synthase (TPS), which is the last link in the synthesis process of terpenoids. <i<Liriodendron chinense,</i< commonly called the Chinese tulip tree, is a rare and endangered tree spec...
Ausführliche Beschreibung
Terpenoids play a key role in plant growth and development, supporting resistance regulation and terpene synthase (TPS), which is the last link in the synthesis process of terpenoids. <i<Liriodendron chinense,</i< commonly called the Chinese tulip tree, is a rare and endangered tree species of the family Magnoliaceae. However, the genome-wide identification of the <i<TPS</i< gene family and its transcriptional responses to development and abiotic stress are still unclear. In the present study, we identified a total of 58 <i<TPS</i< genes throughout the <i<L. chinense</i< genome. A phylogenetic tree analysis showed that they were clustered into five subfamilies and unevenly distributed across six chromosomes. A cis-acting element analysis indicated that <i<LcTPSs</i< were assumed to be highly responsive to stress hormones, such as methyl jasmonate (MeJA) and abscisic acid (ABA). Consistent with this, transcriptome data showed that most <i<LcTPS</i< genes responded to abiotic stress, such as cold, drought, and hot stress, at the transcriptional level. Further analysis showed that <i<LcTPS</i< genes were expressed in a tissue-dependent manner, especially in buds, leaves, and bark. Quantitative reverse transcription PCR (qRT-PCR) analysis confirmed that <i<LcTPS</i< expression was significantly higher in mature leaves compared to young leaves. These results provide a reference for understanding the function and role of the <i<TPS</i< family, laying a foundation for further study of the regulation of <i<TPS</i< in terpenoid biosynthesis in <i<L. chinense</i<. Ausführliche Beschreibung