Production of 5-aminolevulinic acid by cell free multi-enzyme catalysis

• First reported work on cell free production of 5-aminolevulinic acid from succinate and glycine. • Thermostable 5-aminolevulinic acid synthase was used in the cell free process and polyphosphate was used for ATP regeneration to reduce the production cost. • Fed-batch addition of succinate to avoid...
Ausführliche Beschreibung

• First reported work on cell free production of 5-aminolevulinic acid from succinate and glycine. • Thermostable 5-aminolevulinic acid synthase was used in the cell free process and polyphosphate was used for ATP regeneration to reduce the production cost. • Fed-batch addition of succinate to avoid its inhibition on succinyl-CoA synthase. Ausführliche Beschreibung