Advances in methods for native expression and purification of RNA for structural studies

Highlights • Denaturing RNA purification protocols can be unsuitable for various reasons. • RNA can be natively expressed in E. coli using a stabilizing tRNA scaffold. • A series of RNA affinity tags have been developed for native purification. • Simple gel filtration and ion exchange protocols have...
Ausführliche Beschreibung

Highlights • Denaturing RNA purification protocols can be unsuitable for various reasons. • RNA can be natively expressed in E. coli using a stabilizing tRNA scaffold. • A series of RNA affinity tags have been developed for native purification. • Simple gel filtration and ion exchange protocols have been established for RNA. Ausführliche Beschreibung