Inactivation and reactivation of light-triggered ATP hydrolysis on the chloroplast coupling factor

Decay of light-triggered ATP hydrolysis in the dark was diminished with a decrease in chloroplast concentration. The enhancing effect of NH"4Cl on ATP hydrolysis decreased with dark time. The decrease was much faster than that in ATP hydrolysis activity. The NH"4Cl effect increased with AT...
Ausführliche Beschreibung

Decay of light-triggered ATP hydrolysis in the dark was diminished with a decrease in chloroplast concentration. The enhancing effect of NH"4Cl on ATP hydrolysis decreased with dark time. The decrease was much faster than that in ATP hydrolysis activity. The NH"4Cl effect increased with ATP preincubation time. Reactivation of ATP hydrolysis occurred with the progress of ATP hydrolysis. P"i enhanced the activation remarkably. These results suggest that ATP hydrolysis produces some energized state, which stimulates NH"4C1 effect and makes coupling factor active in the presence of P"i and that to keep coupling factor active, energy is not necessarily needed. Ausführliche Beschreibung