Radiorespirometer for continuous monitoring of chick embryo development

A radiorespirometer is described that is capable of continuous monitoring of O"2 utilization, CO"2 and/or ^1^4CO"2 production per minute, heart rate, and body activity of an embryonated egg while it develops for several days in a closed chamber with near normal pO"2 or other desi...
Ausführliche Beschreibung

A radiorespirometer is described that is capable of continuous monitoring of O"2 utilization, CO"2 and/or ^1^4CO"2 production per minute, heart rate, and body activity of an embryonated egg while it develops for several days in a closed chamber with near normal pO"2 or other desired pO"2. Oxygen is supplied to the embryo by an electrolytic cell, and CO"2 is removed by a KOH solution flowing through a diffusion cell separated from the embryo chamber by a CO"2 permeable rubber membrane. The instrument permits selecting embryos for viability and developmental stage, according to their O"2 utilization per minute and respiratory quotient, without breaking the eggshell. Inoculation of the embryonated egg with ^1^4C-labeled substrates, drugs, or toxins can occur without interfering with continuous recording of metabolic activity. Ausführliche Beschreibung