Effect of mutations at the lariat branch acceptor site on β-globin pre-mRNA splicing in vitro

[Auszug] Lariat formation1'5 results from the 2-5' linkage of the 5' end of the intron to an adenosine residue close to the 3' splice site6. In the case of yeast nuclear pre-mRNA splicing, efficient lariat formation depends stringently on the strictly conserved TACTAAC-box, where...
Ausführliche Beschreibung

[Auszug] Lariat formation1'5 results from the 2-5' linkage of the 5' end of the intron to an adenosine residue close to the 3' splice site6. In the case of yeast nuclear pre-mRNA splicing, efficient lariat formation depends stringently on the strictly conserved TACTAAC-box, where the underlined 3'-proximal ... Ausführliche Beschreibung