Alterations in Cerebral and Microvascular Prostaglandin Synthesis by Manipulation of Dietary Essential Fatty Acids

Abstract: Sprague-Dawley rats were fed one of three purified diets—10% corn oil, 10% hydrogenated coconut oil, or 10% linseed oil—through two generations. At 60–80 days of age the animals were sacrificed. The fatty acyl composition of phosphatidylcholine, phosphatidyletha-nolamine, plasmalogen phosp...
Ausführliche Beschreibung

Abstract: Sprague-Dawley rats were fed one of three purified diets—10% corn oil, 10% hydrogenated coconut oil, or 10% linseed oil—through two generations. At 60–80 days of age the animals were sacrificed. The fatty acyl composition of phosphatidylcholine, phosphatidyletha-nolamine, plasmalogen phosphatidylethanolamine, and combined phosphatidylinositol/phosphatidylserine from cerebral cortex and isolated cerebral microvessels was determined. Brain slice prostaglandin F2α or microvas-cular prostacyclin synthesis was also measured. Major changes were noted in the fatty acid profiles, most dramatically in the phosphatidylethanolamine and ethanol-amine plasmalogen fractions, with an active rise in docosahexaenoic acid resulting from linseed oil feeding. A depression in prostaglandin F2α synthesis was seen in brain slices of hydrogenated coconut oil-and linseed oil-fed rats. Such a depression was also observed in micro-vascular prostaglandin synthesis at basal and stimulated levels but not in control incubations. The potential importance of these findings to cerebral microcirculation and hemostasis is discussed. Ausführliche Beschreibung