Emulsan quantitation by Nile red quenching fluorescence assay

Abstract A Nile red fluorescent technique to quantify 20–200 μg $ ml^{−1} $ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 $ cm^{−1} $ $ M^{−1} $. Nile red fluorescence in DMSO was pro...
Ausführliche Beschreibung

Abstract A Nile red fluorescent technique to quantify 20–200 μg $ ml^{−1} $ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 $ cm^{−1} $ $ M^{−1} $. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG-1. Ausführliche Beschreibung