Solid phase extraction with electrospun nanofibers for determination of retinol and α-tocopherol in plasma

Abstract A novel packed-fiber solid phase extraction procedure based on electrospun nanofibers for simultaneous determination of vitamins A (retinol) and E (α-tocopherol) in human plasma has been developed. Parameters affecting extraction efficiency were investigated in detail. The limit of detectio...
Ausführliche Beschreibung

Abstract A novel packed-fiber solid phase extraction procedure based on electrospun nanofibers for simultaneous determination of vitamins A (retinol) and E (α-tocopherol) in human plasma has been developed. Parameters affecting extraction efficiency were investigated in detail. The limit of detection is 0.01 μg $ mL^{−1} $ for retinol, and 0.3 μg $ mL^{−1} $ for α-tocopherol. The linear range is from 0.05 to 2.0 μg $ mL^{−1} $ for retinol, and from 0.5 to 30 μg $ mL^{−1} $ for α-tocopherol. The precision (RSD) is <6%, and the relative recovery >90%. The method was applied to analysis of retinol and α-tocopherol in human plasma with satisfactory results. Ausführliche Beschreibung