Dynamic changes of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) on pulmonary injury induced by ischemia–reperfusion in rats

Objective To evaluate the dynamic changes of PECAM-1/CD31 on pulmonary injury induced by ischemia–reperfusion (IR) in rats. Methods An isolated lung ischemia in rats was performed for 60 min, followed separately by 0, 2, 4, 6 or 8 h for reperfusion. At the end of reperfusion, the expression of PECAM...
Ausführliche Beschreibung

Objective To evaluate the dynamic changes of PECAM-1/CD31 on pulmonary injury induced by ischemia–reperfusion (IR) in rats. Methods An isolated lung ischemia in rats was performed for 60 min, followed separately by 0, 2, 4, 6 or 8 h for reperfusion. At the end of reperfusion, the expression of PECAM-1/CD31 was detected by using flow cytometry (FCM). Simultaneously, we examined the ultrastructure changes of cells by transmission electron microscope. Results The expressions of PECAM-1/CD31 in neutrophils and lymphocytes of the blood were increased after IR; the peak was at 2 h after IR, then the expressions were gradually decreased to control level at 6 and 8 h after IR. Ultrastructural morphological changes including proliferation of alveolar type II and apoptotic bodies were seen after IR, the most prominently at 2 h after IR. Conclusion PECAM-1/CD31 is involved in the pulmonary injury induced by IR. Ausführliche Beschreibung