Modulation of rabbit mitochondrial nuclease activity by S-adenosyl-L-methionine

Abstract Endonuclease activity with properties similar to those of the animal endonuclease G has been detected in extracts of rabbit liver mitochondria. This activity was detected in the fraction of proteins with molecular mass close to 30 kDa; it was stimulated by $ Mg^{2+} $ ions and inhibited by...
Ausführliche Beschreibung

Abstract Endonuclease activity with properties similar to those of the animal endonuclease G has been detected in extracts of rabbit liver mitochondria. This activity was detected in the fraction of proteins with molecular mass close to 30 kDa; it was stimulated by $ Mg^{2+} $ ions and inhibited by $ Zn^{2+} $ ions. In contrast to plant endonucleases WEN1 and WEN2, the rabbit endonuclease was not affected by methylation status of the substrate DNA, and S-adenosine-L-methionine inhibited it. Ausführliche Beschreibung