Effect of free fatty acids and cholesterol in vitro on liver plasma membrane-bound enzymes

Summary The effect of cholesterol and fatty acid treatment in vitro was tested on rat liver plasma membrane-bound enzymes and lipid fluidity. The observed alterations of membrane fluidity affect both ($ Na^{+} $−$ K^{+} $)-ATPase and $ Mg^{2+} $-ATPase activities but not 5′-nucleotidase; basal adeny...
Ausführliche Beschreibung

Summary The effect of cholesterol and fatty acid treatment in vitro was tested on rat liver plasma membrane-bound enzymes and lipid fluidity. The observed alterations of membrane fluidity affect both ($ Na^{+} $−$ K^{+} $)-ATPase and $ Mg^{2+} $-ATPase activities but not 5′-nucleotidase; basal adenylate cyclase as well as its hormonal sensitivity were differentially affected by changes of membrane microenvironment. Ausführliche Beschreibung