A nonenzymic oxygen uptake and its implications in the assay of 4-hydroxyphenylpyruvate dioxygenase by an oxygen electrode

Summary The oxygen electrode provides a rapid, convenient assay for 4-hydroxyphenylpyruvate dioxygenase. However, due to the occurrence of a nonenzymic oxygen consumption when reducing agents were mixed witha,a′-dipyridyl, its use is restricted to species which do not require reducing agents or nece...
Ausführliche Beschreibung

Summary The oxygen electrode provides a rapid, convenient assay for 4-hydroxyphenylpyruvate dioxygenase. However, due to the occurrence of a nonenzymic oxygen consumption when reducing agents were mixed witha,a′-dipyridyl, its use is restricted to species which do not require reducing agents or necessitates the addition of catalase to the reaction mixture. Ausführliche Beschreibung