Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A

The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Kais, Britta [verfasserIn] Ottermanns, Richard - 1967- [verfasserIn] Scheller, Franziska [verfasserIn] Braunbeck, Thomas [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	15 February 2018

Schlagwörter:	CYP1A inhibition Densitometry EROD induction Live-imaging Zebrafish embryo

Anmerkung:	Available online: 2 October 2017 Gesehen am 21.02.2018
Umfang:	18

Weitere Ausgabe:	Erscheint auch als Druck-Ausgabe Kais, Britta: Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A - 2018
Übergeordnetes Werk:	Enthalten in: The science of the total environment - Amsterdam [u.a.] : Elsevier Science, 1972, 615(2018), Seite 330-347
Übergeordnetes Werk:	volume:615 ; year:2018 ; pages:330-347 ; extent:18

Links:	Volltext Volltext

DOI / URN:	10.1016/j.scitotenv.2017.09.257

Katalog-ID:	157007027X

Internformat


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520			\|a The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials.
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Indexfelder

author_variant	b k bk r o ro f s fs t b tb
matchkey_str	article:18791026:2018----::oiiainnqatfctooivveolviaigiherfsdnoeiebysoeet
oclc_num	1340992052
hierarchy_sort_str	15 February 2018
publishDate	2018
allfields	10.1016/j.scitotenv.2017.09.257 doi (DE-627)157007027X (DE-576)50007027X (DE-599)BSZ50007027X (OCoLC)1340992052 DE-627 ger DE-627 rda eng Kais, Britta verfasserin (DE-588)1069019496 (DE-627)821169122 (DE-576)428367216 aut Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck 15 February 2018 18 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Available online: 2 October 2017 Gesehen am 21.02.2018 The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials. CYP1A inhibition Densitometry EROD induction Live-imaging Zebrafish embryo Ottermanns, Richard 1967- verfasserin (DE-588)136453597 (DE-627)583297013 (DE-576)301028516 aut Scheller, Franziska verfasserin (DE-588)1162873094 (DE-627)1026977835 (DE-576)50764834X aut Braunbeck, Thomas verfasserin (DE-588)106835819X (DE-627)82017839X (DE-576)165906731 aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 615(2018), Seite 330-347 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:615 year:2018 pages:330-347 extent:18 Erscheint auch als Druck-Ausgabe Kais, Britta Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A 2018 (DE-627)1570071292 (DE-576)500071292 http://dx.doi.org/10.1016/j.scitotenv.2017.09.257 Verlag Resolving-System Volltext http://www.sciencedirect.com/science/article/pii/S0048969717326098 Verlag Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 615 2018 330-347 18 2013 01 DE-16-250 3000265864 00 --%%-- --%%-- --%%-- --%%-- l01 21-02-18 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_18 2013 01 DE-16-250 04 p (DE-627)1498367224 Kais, Britta 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)157764851X Scheller, Franziska 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1497762731 Braunbeck, Thomas 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 k (DE-627)1449800602 Heidelberg Center for the Environment (HCE) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4
spelling	10.1016/j.scitotenv.2017.09.257 doi (DE-627)157007027X (DE-576)50007027X (DE-599)BSZ50007027X (OCoLC)1340992052 DE-627 ger DE-627 rda eng Kais, Britta verfasserin (DE-588)1069019496 (DE-627)821169122 (DE-576)428367216 aut Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck 15 February 2018 18 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Available online: 2 October 2017 Gesehen am 21.02.2018 The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials. CYP1A inhibition Densitometry EROD induction Live-imaging Zebrafish embryo Ottermanns, Richard 1967- verfasserin (DE-588)136453597 (DE-627)583297013 (DE-576)301028516 aut Scheller, Franziska verfasserin (DE-588)1162873094 (DE-627)1026977835 (DE-576)50764834X aut Braunbeck, Thomas verfasserin (DE-588)106835819X (DE-627)82017839X (DE-576)165906731 aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 615(2018), Seite 330-347 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:615 year:2018 pages:330-347 extent:18 Erscheint auch als Druck-Ausgabe Kais, Britta Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A 2018 (DE-627)1570071292 (DE-576)500071292 http://dx.doi.org/10.1016/j.scitotenv.2017.09.257 Verlag Resolving-System Volltext http://www.sciencedirect.com/science/article/pii/S0048969717326098 Verlag Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 615 2018 330-347 18 2013 01 DE-16-250 3000265864 00 --%%-- --%%-- --%%-- --%%-- l01 21-02-18 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_18 2013 01 DE-16-250 04 p (DE-627)1498367224 Kais, Britta 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)157764851X Scheller, Franziska 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1497762731 Braunbeck, Thomas 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 k (DE-627)1449800602 Heidelberg Center for the Environment (HCE) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4
allfields_unstemmed	10.1016/j.scitotenv.2017.09.257 doi (DE-627)157007027X (DE-576)50007027X (DE-599)BSZ50007027X (OCoLC)1340992052 DE-627 ger DE-627 rda eng Kais, Britta verfasserin (DE-588)1069019496 (DE-627)821169122 (DE-576)428367216 aut Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck 15 February 2018 18 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Available online: 2 October 2017 Gesehen am 21.02.2018 The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials. CYP1A inhibition Densitometry EROD induction Live-imaging Zebrafish embryo Ottermanns, Richard 1967- verfasserin (DE-588)136453597 (DE-627)583297013 (DE-576)301028516 aut Scheller, Franziska verfasserin (DE-588)1162873094 (DE-627)1026977835 (DE-576)50764834X aut Braunbeck, Thomas verfasserin (DE-588)106835819X (DE-627)82017839X (DE-576)165906731 aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 615(2018), Seite 330-347 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:615 year:2018 pages:330-347 extent:18 Erscheint auch als Druck-Ausgabe Kais, Britta Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A 2018 (DE-627)1570071292 (DE-576)500071292 http://dx.doi.org/10.1016/j.scitotenv.2017.09.257 Verlag Resolving-System Volltext http://www.sciencedirect.com/science/article/pii/S0048969717326098 Verlag Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 615 2018 330-347 18 2013 01 DE-16-250 3000265864 00 --%%-- --%%-- --%%-- --%%-- l01 21-02-18 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_18 2013 01 DE-16-250 04 p (DE-627)1498367224 Kais, Britta 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)157764851X Scheller, Franziska 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1497762731 Braunbeck, Thomas 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 k (DE-627)1449800602 Heidelberg Center for the Environment (HCE) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4
allfieldsGer	10.1016/j.scitotenv.2017.09.257 doi (DE-627)157007027X (DE-576)50007027X (DE-599)BSZ50007027X (OCoLC)1340992052 DE-627 ger DE-627 rda eng Kais, Britta verfasserin (DE-588)1069019496 (DE-627)821169122 (DE-576)428367216 aut Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck 15 February 2018 18 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Available online: 2 October 2017 Gesehen am 21.02.2018 The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials. CYP1A inhibition Densitometry EROD induction Live-imaging Zebrafish embryo Ottermanns, Richard 1967- verfasserin (DE-588)136453597 (DE-627)583297013 (DE-576)301028516 aut Scheller, Franziska verfasserin (DE-588)1162873094 (DE-627)1026977835 (DE-576)50764834X aut Braunbeck, Thomas verfasserin (DE-588)106835819X (DE-627)82017839X (DE-576)165906731 aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 615(2018), Seite 330-347 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:615 year:2018 pages:330-347 extent:18 Erscheint auch als Druck-Ausgabe Kais, Britta Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A 2018 (DE-627)1570071292 (DE-576)500071292 http://dx.doi.org/10.1016/j.scitotenv.2017.09.257 Verlag Resolving-System Volltext http://www.sciencedirect.com/science/article/pii/S0048969717326098 Verlag Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 615 2018 330-347 18 2013 01 DE-16-250 3000265864 00 --%%-- --%%-- --%%-- --%%-- l01 21-02-18 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_18 2013 01 DE-16-250 04 p (DE-627)1498367224 Kais, Britta 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)157764851X Scheller, Franziska 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1497762731 Braunbeck, Thomas 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 k (DE-627)1449800602 Heidelberg Center for the Environment (HCE) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4
allfieldsSound	10.1016/j.scitotenv.2017.09.257 doi (DE-627)157007027X (DE-576)50007027X (DE-599)BSZ50007027X (OCoLC)1340992052 DE-627 ger DE-627 rda eng Kais, Britta verfasserin (DE-588)1069019496 (DE-627)821169122 (DE-576)428367216 aut Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck 15 February 2018 18 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Available online: 2 October 2017 Gesehen am 21.02.2018 The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials. CYP1A inhibition Densitometry EROD induction Live-imaging Zebrafish embryo Ottermanns, Richard 1967- verfasserin (DE-588)136453597 (DE-627)583297013 (DE-576)301028516 aut Scheller, Franziska verfasserin (DE-588)1162873094 (DE-627)1026977835 (DE-576)50764834X aut Braunbeck, Thomas verfasserin (DE-588)106835819X (DE-627)82017839X (DE-576)165906731 aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 615(2018), Seite 330-347 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:615 year:2018 pages:330-347 extent:18 Erscheint auch als Druck-Ausgabe Kais, Britta Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A 2018 (DE-627)1570071292 (DE-576)500071292 http://dx.doi.org/10.1016/j.scitotenv.2017.09.257 Verlag Resolving-System Volltext http://www.sciencedirect.com/science/article/pii/S0048969717326098 Verlag Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 615 2018 330-347 18 2013 01 DE-16-250 3000265864 00 --%%-- --%%-- --%%-- --%%-- l01 21-02-18 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_18 2013 01 DE-16-250 04 p (DE-627)1498367224 Kais, Britta 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)157764851X Scheller, Franziska 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1497762731 Braunbeck, Thomas 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 k (DE-627)1449800602 Heidelberg Center for the Environment (HCE) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4
language	English
source	Enthalten in The science of the total environment 615(2018), Seite 330-347 volume:615 year:2018 pages:330-347 extent:18
sourceStr	Enthalten in The science of the total environment 615(2018), Seite 330-347 volume:615 year:2018 pages:330-347 extent:18
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topic_facet	CYP1A inhibition Densitometry EROD induction Live-imaging Zebrafish embryo
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container_title	The science of the total environment
authorswithroles_txt_mv	Kais, Britta @@aut@@ Ottermanns, Richard @@aut@@ Scheller, Franziska @@aut@@ Braunbeck, Thomas @@aut@@
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fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">157007027X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20220814080823.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180221s2018 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.scitotenv.2017.09.257</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)157007027X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-576)50007027X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BSZ50007027X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1340992052</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Kais, Britta</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(DE-588)1069019496</subfield><subfield code="0">(DE-627)821169122</subfield><subfield code="0">(DE-576)428367216</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A</subfield><subfield code="c">Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">15 February 2018</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">18</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Available online: 2 October 2017</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Gesehen am 21.02.2018</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. 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spellingShingle	Kais, Britta misc CYP1A inhibition misc Densitometry misc EROD induction misc Live-imaging misc Zebrafish embryo 2013 hd2018 2013 wissenschaftlicher Artikel (Zeitschrift) 2013 per_4 2013 s_18 2013 Kais, Britta 2013 Centre for Organismal Studies Heidelberg (COS) 2013 Verfasser 2013 pos_1 2013 Scheller, Franziska 2013 pos_3 2013 Braunbeck, Thomas 2013 Heidelberg Center for the Environment (HCE) 2013 pos_4 Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A
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topic_title	2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_18 2013 01 DE-16-250 04 p (DE-627)1498367224 Kais, Britta 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)157764851X Scheller, Franziska 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1497762731 Braunbeck, Thomas 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 k (DE-627)1449800602 Heidelberg Center for the Environment (HCE) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck CYP1A inhibition Densitometry EROD induction Live-imaging Zebrafish embryo
topic	misc CYP1A inhibition misc Densitometry misc EROD induction misc Live-imaging misc Zebrafish embryo 2013 hd2018 2013 wissenschaftlicher Artikel (Zeitschrift) 2013 per_4 2013 s_18 2013 Kais, Britta 2013 Centre for Organismal Studies Heidelberg (COS) 2013 Verfasser 2013 pos_1 2013 Scheller, Franziska 2013 pos_3 2013 Braunbeck, Thomas 2013 Heidelberg Center for the Environment (HCE) 2013 pos_4
topic_unstemmed	misc CYP1A inhibition misc Densitometry misc EROD induction misc Live-imaging misc Zebrafish embryo 2013 hd2018 2013 wissenschaftlicher Artikel (Zeitschrift) 2013 per_4 2013 s_18 2013 Kais, Britta 2013 Centre for Organismal Studies Heidelberg (COS) 2013 Verfasser 2013 pos_1 2013 Scheller, Franziska 2013 pos_3 2013 Braunbeck, Thomas 2013 Heidelberg Center for the Environment (HCE) 2013 pos_4
topic_browse	misc CYP1A inhibition misc Densitometry misc EROD induction misc Live-imaging misc Zebrafish embryo 2013 hd2018 2013 wissenschaftlicher Artikel (Zeitschrift) 2013 per_4 2013 s_18 2013 Kais, Britta 2013 Centre for Organismal Studies Heidelberg (COS) 2013 Verfasser 2013 pos_1 2013 Scheller, Franziska 2013 pos_3 2013 Braunbeck, Thomas 2013 Heidelberg Center for the Environment (HCE) 2013 pos_4
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title	Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A
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title_full	Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck
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title_sort	modification and quantification of in vivo erod live-imaging with zebrafish (danio rerio) embryos to detect both induction and inhibition of cyp1a
title_auth	Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A
abstract	The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials. Available online: 2 October 2017 Gesehen am 21.02.2018
abstractGer	The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials. Available online: 2 October 2017 Gesehen am 21.02.2018
abstract_unstemmed	The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials. Available online: 2 October 2017 Gesehen am 21.02.2018
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title_short	Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A
url	http://dx.doi.org/10.1016/j.scitotenv.2017.09.257 http://www.sciencedirect.com/science/article/pii/S0048969717326098
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Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A

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