Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection

Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice...
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Autor*in:	Bortz, Eric [verfasserIn] Wu, Ting-Ting [verfasserIn] Patel, Parthive H. [verfasserIn] Whitelegge, Julian P. [verfasserIn] Sun, Ren [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	27 November 2018

Schlagwörter:	BAL bronchoalveolar lavage fluid MHV-68 murine herpesvirus-68 oxidative stress proteomics

Anmerkung:	Gesehen am 30.03.2020
Umfang:	24

Übergeordnetes Werk:	Enthalten in: Viruses - Basel : MDPI, 2009, 10(2018,12) Artikel-Nummer 670, 24 Seiten
Übergeordnetes Werk:	volume:10 ; year:2018 ; number:12 ; extent:24

Links:	Volltext Volltext

DOI / URN:	10.3390/v10120670

Katalog-ID:	169347719X

Internformat


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allfields	10.3390/v10120670 doi (DE-627)169347719X (DE-599)KXP169347719X (OCoLC)1341311591 DE-627 ger DE-627 rda eng Bortz, Eric verfasserin (DE-588)1207285641 (DE-627)1693478188 aut Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection Eric Bortz, Ting-Ting Wu, Parthive Patel, Julian P. Whitelegge and Ren Sun 27 November 2018 24 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 30.03.2020 Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice were infected intranasally with MHV-68. After nine days, as the lytic phase of infection resolved, differential BAL proteins were identified by two-dimensional (2D) electrophoresis and mass spectrometry. Of 23 unique proteins, acute phase proteins, vitamin A transport, and oxidative stress response factors Pdx6 and EC-SOD (Sod3) were enriched. Correspondingly, iNOS2 was induced in lung tissue by seven days post-infection. Oxidative stress was partly a direct result of MHV-68 infection, as reactive oxygen species (ROS) were induced in cultured murine NIH3T3 fibroblasts and human lung A549 cells infected with MHV-68. Finally, mice infected with a recombinant MHV-68 co-expressing inflammatory cytokine murine interleukin 6 (IL6) showed exacerbated oxidative stress and soluble type I collagen characteristic of tissue recovery. Thus, oxidative stress appears to be a salient feature of MHV-68 pathogenesis, in part caused by lytic replication of the virus and IL6. Proteins and small molecules in lung oxidative stress networks therefore may provide new therapeutic targets to ameliorate respiratory virus infections. BAL bronchoalveolar lavage fluid MHV-68 murine herpesvirus-68 oxidative stress proteomics Wu, Ting-Ting verfasserin aut Patel, Parthive H. verfasserin (DE-588)1165665743 (DE-627)1029734690 (DE-576)510487483 aut Whitelegge, Julian P. verfasserin aut Sun, Ren verfasserin aut Enthalten in Viruses Basel : MDPI, 2009 10(2018,12) Artikel-Nummer 670, 24 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:10 year:2018 number:12 extent:24 https://doi.org/10.3390/v10120670 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/10/12/670 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 12 24 10(2018,12) Artikel-Nummer 670, 24 Seiten 2013 01 DE-16-250 3615660056 00 --%%-- --%%-- --%%-- --%%-- l01 30-03-20 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_5 2013 01 DE-16-250 03 s s_24 2013 01 DE-16-250 04 p (DE-627)1580488528 Patel, Parthive H. 2013 01 DE-16-250 04 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_3
spelling	10.3390/v10120670 doi (DE-627)169347719X (DE-599)KXP169347719X (OCoLC)1341311591 DE-627 ger DE-627 rda eng Bortz, Eric verfasserin (DE-588)1207285641 (DE-627)1693478188 aut Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection Eric Bortz, Ting-Ting Wu, Parthive Patel, Julian P. Whitelegge and Ren Sun 27 November 2018 24 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 30.03.2020 Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice were infected intranasally with MHV-68. After nine days, as the lytic phase of infection resolved, differential BAL proteins were identified by two-dimensional (2D) electrophoresis and mass spectrometry. Of 23 unique proteins, acute phase proteins, vitamin A transport, and oxidative stress response factors Pdx6 and EC-SOD (Sod3) were enriched. Correspondingly, iNOS2 was induced in lung tissue by seven days post-infection. Oxidative stress was partly a direct result of MHV-68 infection, as reactive oxygen species (ROS) were induced in cultured murine NIH3T3 fibroblasts and human lung A549 cells infected with MHV-68. Finally, mice infected with a recombinant MHV-68 co-expressing inflammatory cytokine murine interleukin 6 (IL6) showed exacerbated oxidative stress and soluble type I collagen characteristic of tissue recovery. Thus, oxidative stress appears to be a salient feature of MHV-68 pathogenesis, in part caused by lytic replication of the virus and IL6. Proteins and small molecules in lung oxidative stress networks therefore may provide new therapeutic targets to ameliorate respiratory virus infections. BAL bronchoalveolar lavage fluid MHV-68 murine herpesvirus-68 oxidative stress proteomics Wu, Ting-Ting verfasserin aut Patel, Parthive H. verfasserin (DE-588)1165665743 (DE-627)1029734690 (DE-576)510487483 aut Whitelegge, Julian P. verfasserin aut Sun, Ren verfasserin aut Enthalten in Viruses Basel : MDPI, 2009 10(2018,12) Artikel-Nummer 670, 24 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:10 year:2018 number:12 extent:24 https://doi.org/10.3390/v10120670 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/10/12/670 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 12 24 10(2018,12) Artikel-Nummer 670, 24 Seiten 2013 01 DE-16-250 3615660056 00 --%%-- --%%-- --%%-- --%%-- l01 30-03-20 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_5 2013 01 DE-16-250 03 s s_24 2013 01 DE-16-250 04 p (DE-627)1580488528 Patel, Parthive H. 2013 01 DE-16-250 04 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_3
allfields_unstemmed	10.3390/v10120670 doi (DE-627)169347719X (DE-599)KXP169347719X (OCoLC)1341311591 DE-627 ger DE-627 rda eng Bortz, Eric verfasserin (DE-588)1207285641 (DE-627)1693478188 aut Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection Eric Bortz, Ting-Ting Wu, Parthive Patel, Julian P. Whitelegge and Ren Sun 27 November 2018 24 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 30.03.2020 Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice were infected intranasally with MHV-68. After nine days, as the lytic phase of infection resolved, differential BAL proteins were identified by two-dimensional (2D) electrophoresis and mass spectrometry. Of 23 unique proteins, acute phase proteins, vitamin A transport, and oxidative stress response factors Pdx6 and EC-SOD (Sod3) were enriched. Correspondingly, iNOS2 was induced in lung tissue by seven days post-infection. Oxidative stress was partly a direct result of MHV-68 infection, as reactive oxygen species (ROS) were induced in cultured murine NIH3T3 fibroblasts and human lung A549 cells infected with MHV-68. Finally, mice infected with a recombinant MHV-68 co-expressing inflammatory cytokine murine interleukin 6 (IL6) showed exacerbated oxidative stress and soluble type I collagen characteristic of tissue recovery. Thus, oxidative stress appears to be a salient feature of MHV-68 pathogenesis, in part caused by lytic replication of the virus and IL6. Proteins and small molecules in lung oxidative stress networks therefore may provide new therapeutic targets to ameliorate respiratory virus infections. BAL bronchoalveolar lavage fluid MHV-68 murine herpesvirus-68 oxidative stress proteomics Wu, Ting-Ting verfasserin aut Patel, Parthive H. verfasserin (DE-588)1165665743 (DE-627)1029734690 (DE-576)510487483 aut Whitelegge, Julian P. verfasserin aut Sun, Ren verfasserin aut Enthalten in Viruses Basel : MDPI, 2009 10(2018,12) Artikel-Nummer 670, 24 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:10 year:2018 number:12 extent:24 https://doi.org/10.3390/v10120670 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/10/12/670 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 12 24 10(2018,12) Artikel-Nummer 670, 24 Seiten 2013 01 DE-16-250 3615660056 00 --%%-- --%%-- --%%-- --%%-- l01 30-03-20 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_5 2013 01 DE-16-250 03 s s_24 2013 01 DE-16-250 04 p (DE-627)1580488528 Patel, Parthive H. 2013 01 DE-16-250 04 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_3
allfieldsGer	10.3390/v10120670 doi (DE-627)169347719X (DE-599)KXP169347719X (OCoLC)1341311591 DE-627 ger DE-627 rda eng Bortz, Eric verfasserin (DE-588)1207285641 (DE-627)1693478188 aut Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection Eric Bortz, Ting-Ting Wu, Parthive Patel, Julian P. Whitelegge and Ren Sun 27 November 2018 24 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 30.03.2020 Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice were infected intranasally with MHV-68. After nine days, as the lytic phase of infection resolved, differential BAL proteins were identified by two-dimensional (2D) electrophoresis and mass spectrometry. Of 23 unique proteins, acute phase proteins, vitamin A transport, and oxidative stress response factors Pdx6 and EC-SOD (Sod3) were enriched. Correspondingly, iNOS2 was induced in lung tissue by seven days post-infection. Oxidative stress was partly a direct result of MHV-68 infection, as reactive oxygen species (ROS) were induced in cultured murine NIH3T3 fibroblasts and human lung A549 cells infected with MHV-68. Finally, mice infected with a recombinant MHV-68 co-expressing inflammatory cytokine murine interleukin 6 (IL6) showed exacerbated oxidative stress and soluble type I collagen characteristic of tissue recovery. Thus, oxidative stress appears to be a salient feature of MHV-68 pathogenesis, in part caused by lytic replication of the virus and IL6. Proteins and small molecules in lung oxidative stress networks therefore may provide new therapeutic targets to ameliorate respiratory virus infections. BAL bronchoalveolar lavage fluid MHV-68 murine herpesvirus-68 oxidative stress proteomics Wu, Ting-Ting verfasserin aut Patel, Parthive H. verfasserin (DE-588)1165665743 (DE-627)1029734690 (DE-576)510487483 aut Whitelegge, Julian P. verfasserin aut Sun, Ren verfasserin aut Enthalten in Viruses Basel : MDPI, 2009 10(2018,12) Artikel-Nummer 670, 24 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:10 year:2018 number:12 extent:24 https://doi.org/10.3390/v10120670 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/10/12/670 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 12 24 10(2018,12) Artikel-Nummer 670, 24 Seiten 2013 01 DE-16-250 3615660056 00 --%%-- --%%-- --%%-- --%%-- l01 30-03-20 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_5 2013 01 DE-16-250 03 s s_24 2013 01 DE-16-250 04 p (DE-627)1580488528 Patel, Parthive H. 2013 01 DE-16-250 04 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_3
allfieldsSound	10.3390/v10120670 doi (DE-627)169347719X (DE-599)KXP169347719X (OCoLC)1341311591 DE-627 ger DE-627 rda eng Bortz, Eric verfasserin (DE-588)1207285641 (DE-627)1693478188 aut Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection Eric Bortz, Ting-Ting Wu, Parthive Patel, Julian P. Whitelegge and Ren Sun 27 November 2018 24 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 30.03.2020 Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice were infected intranasally with MHV-68. After nine days, as the lytic phase of infection resolved, differential BAL proteins were identified by two-dimensional (2D) electrophoresis and mass spectrometry. Of 23 unique proteins, acute phase proteins, vitamin A transport, and oxidative stress response factors Pdx6 and EC-SOD (Sod3) were enriched. Correspondingly, iNOS2 was induced in lung tissue by seven days post-infection. Oxidative stress was partly a direct result of MHV-68 infection, as reactive oxygen species (ROS) were induced in cultured murine NIH3T3 fibroblasts and human lung A549 cells infected with MHV-68. Finally, mice infected with a recombinant MHV-68 co-expressing inflammatory cytokine murine interleukin 6 (IL6) showed exacerbated oxidative stress and soluble type I collagen characteristic of tissue recovery. Thus, oxidative stress appears to be a salient feature of MHV-68 pathogenesis, in part caused by lytic replication of the virus and IL6. Proteins and small molecules in lung oxidative stress networks therefore may provide new therapeutic targets to ameliorate respiratory virus infections. BAL bronchoalveolar lavage fluid MHV-68 murine herpesvirus-68 oxidative stress proteomics Wu, Ting-Ting verfasserin aut Patel, Parthive H. verfasserin (DE-588)1165665743 (DE-627)1029734690 (DE-576)510487483 aut Whitelegge, Julian P. verfasserin aut Sun, Ren verfasserin aut Enthalten in Viruses Basel : MDPI, 2009 10(2018,12) Artikel-Nummer 670, 24 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:10 year:2018 number:12 extent:24 https://doi.org/10.3390/v10120670 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/10/12/670 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 12 24 10(2018,12) Artikel-Nummer 670, 24 Seiten 2013 01 DE-16-250 3615660056 00 --%%-- --%%-- --%%-- --%%-- l01 30-03-20 2013 01 DE-16-250 00 s hd2018 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_5 2013 01 DE-16-250 03 s s_24 2013 01 DE-16-250 04 p (DE-627)1580488528 Patel, Parthive H. 2013 01 DE-16-250 04 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_3
language	English
source	Enthalten in Viruses 10(2018,12) Artikel-Nummer 670, 24 Seiten volume:10 year:2018 number:12 extent:24
sourceStr	Enthalten in Viruses 10(2018,12) Artikel-Nummer 670, 24 Seiten volume:10 year:2018 number:12 extent:24
format_phy_str_mv	Article
building	2013:0
institution	findex.gbv.de
selectbib_iln_str_mv	2013@01
topic_facet	BAL bronchoalveolar lavage fluid MHV-68 murine herpesvirus-68 oxidative stress proteomics
sw_local_iln_str_mv	2013:hd2018 DE-16-250:hd2018 2013:wissenschaftlicher Artikel (Zeitschrift) DE-16-250:wissenschaftlicher Artikel (Zeitschrift) 2013:per_5 DE-16-250:per_5 2013:s_24 DE-16-250:s_24 2013:Patel, Parthive H. DE-16-250:Patel, Parthive H. 2013:Zentrum für Molekulare Biologie (ZMBH) DE-16-250:Zentrum für Molekulare Biologie (ZMBH) 2013:Verfasser DE-16-250:Verfasser 2013:pos_3 DE-16-250:pos_3
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container_title	Viruses
authorswithroles_txt_mv	Bortz, Eric @@aut@@ Wu, Ting-Ting @@aut@@ Patel, Parthive H. @@aut@@ Whitelegge, Julian P. @@aut@@ Sun, Ren @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
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topic	misc BAL misc bronchoalveolar lavage fluid misc MHV-68 misc murine herpesvirus-68 misc oxidative stress misc proteomics 2013 hd2018 2013 wissenschaftlicher Artikel (Zeitschrift) 2013 per_5 2013 s_24 2013 Patel, Parthive H. 2013 Zentrum für Molekulare Biologie (ZMBH) 2013 Verfasser 2013 pos_3
topic_unstemmed	misc BAL misc bronchoalveolar lavage fluid misc MHV-68 misc murine herpesvirus-68 misc oxidative stress misc proteomics 2013 hd2018 2013 wissenschaftlicher Artikel (Zeitschrift) 2013 per_5 2013 s_24 2013 Patel, Parthive H. 2013 Zentrum für Molekulare Biologie (ZMBH) 2013 Verfasser 2013 pos_3
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title	Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection
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title_full	Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection Eric Bortz, Ting-Ting Wu, Parthive Patel, Julian P. Whitelegge and Ren Sun
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title_sort	proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection
title_auth	Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection
abstract	Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice were infected intranasally with MHV-68. After nine days, as the lytic phase of infection resolved, differential BAL proteins were identified by two-dimensional (2D) electrophoresis and mass spectrometry. Of 23 unique proteins, acute phase proteins, vitamin A transport, and oxidative stress response factors Pdx6 and EC-SOD (Sod3) were enriched. Correspondingly, iNOS2 was induced in lung tissue by seven days post-infection. Oxidative stress was partly a direct result of MHV-68 infection, as reactive oxygen species (ROS) were induced in cultured murine NIH3T3 fibroblasts and human lung A549 cells infected with MHV-68. Finally, mice infected with a recombinant MHV-68 co-expressing inflammatory cytokine murine interleukin 6 (IL6) showed exacerbated oxidative stress and soluble type I collagen characteristic of tissue recovery. Thus, oxidative stress appears to be a salient feature of MHV-68 pathogenesis, in part caused by lytic replication of the virus and IL6. Proteins and small molecules in lung oxidative stress networks therefore may provide new therapeutic targets to ameliorate respiratory virus infections. Gesehen am 30.03.2020
abstractGer	Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice were infected intranasally with MHV-68. After nine days, as the lytic phase of infection resolved, differential BAL proteins were identified by two-dimensional (2D) electrophoresis and mass spectrometry. Of 23 unique proteins, acute phase proteins, vitamin A transport, and oxidative stress response factors Pdx6 and EC-SOD (Sod3) were enriched. Correspondingly, iNOS2 was induced in lung tissue by seven days post-infection. Oxidative stress was partly a direct result of MHV-68 infection, as reactive oxygen species (ROS) were induced in cultured murine NIH3T3 fibroblasts and human lung A549 cells infected with MHV-68. Finally, mice infected with a recombinant MHV-68 co-expressing inflammatory cytokine murine interleukin 6 (IL6) showed exacerbated oxidative stress and soluble type I collagen characteristic of tissue recovery. Thus, oxidative stress appears to be a salient feature of MHV-68 pathogenesis, in part caused by lytic replication of the virus and IL6. Proteins and small molecules in lung oxidative stress networks therefore may provide new therapeutic targets to ameliorate respiratory virus infections. Gesehen am 30.03.2020
abstract_unstemmed	Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice were infected intranasally with MHV-68. After nine days, as the lytic phase of infection resolved, differential BAL proteins were identified by two-dimensional (2D) electrophoresis and mass spectrometry. Of 23 unique proteins, acute phase proteins, vitamin A transport, and oxidative stress response factors Pdx6 and EC-SOD (Sod3) were enriched. Correspondingly, iNOS2 was induced in lung tissue by seven days post-infection. Oxidative stress was partly a direct result of MHV-68 infection, as reactive oxygen species (ROS) were induced in cultured murine NIH3T3 fibroblasts and human lung A549 cells infected with MHV-68. Finally, mice infected with a recombinant MHV-68 co-expressing inflammatory cytokine murine interleukin 6 (IL6) showed exacerbated oxidative stress and soluble type I collagen characteristic of tissue recovery. Thus, oxidative stress appears to be a salient feature of MHV-68 pathogenesis, in part caused by lytic replication of the virus and IL6. Proteins and small molecules in lung oxidative stress networks therefore may provide new therapeutic targets to ameliorate respiratory virus infections. Gesehen am 30.03.2020
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title_short	Proteomics of bronchoalveolar lavage fluid reveals a lung oxidative stress response in murine herpesvirus-68 infection
url	https://doi.org/10.3390/v10120670 https://www.mdpi.com/1999-4915/10/12/670
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