SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP
Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which t...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Klein, Steffen - 1989- [verfasserIn] Müller, Thorsten G. - 1986- [verfasserIn] Khalid, Dina - 1980- [verfasserIn] Sonntag-Buck, Vera [verfasserIn] Heuser, Anke-Mareil [verfasserIn] Glass, Bärbel [verfasserIn] Meurer, Matthias [verfasserIn] Morales, Ivonne [verfasserIn] Schillak, Angelika [verfasserIn] Freistaedter, Andrew [verfasserIn] Ambiel, Ina [verfasserIn] Winter, Sophie L. - 1992- [verfasserIn] Zimmermann, Liv [verfasserIn] Naumoska, Tamara [verfasserIn] Bubeck, Felix [verfasserIn] Kirrmaier, Daniel [verfasserIn] Ullrich, Stephanie [verfasserIn] Barreto Miranda, Isabel - 1975- [verfasserIn] Anders, Simon [verfasserIn] Grimm, Dirk [verfasserIn] Schnitzler, Paul [verfasserIn] Knop, Michael [verfasserIn] Kräusslich, Hans-Georg - 1958- [verfasserIn] Dao Thi, Viet Loan [verfasserIn] Börner, Kathleen [verfasserIn] Chlanda, Petr [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
7 August 2020 |
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| Schlagwörter: |
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| Anmerkung: |
Gesehen am 03.12.2020 |
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| Umfang: |
13 |
| Übergeordnetes Werk: |
Enthalten in: Viruses - Basel : MDPI, 2009, 12(2020,8) Artikel-Nummer 863, 13 Seiten |
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| Übergeordnetes Werk: |
volume:12 ; year:2020 ; number:8 ; extent:13 |
| Links: |
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| DOI / URN: |
10.3390/v12080863 |
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| Katalog-ID: |
1741841836 |
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| 100 | 1 | |a Klein, Steffen |d 1989- |e verfasserin |0 (DE-588)1209782782 |0 (DE-627)1697757294 |4 aut | |
| 245 | 1 | 0 | |a SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP |c Steffen Klein, Thorsten G. Müller, Dina Khalid, Vera Sonntag-Buck, Anke-Mareil Heuser, Bärbel Glass, Matthias Meurer, Ivonne Morales, Angelika Schillak, Andrew Freistaedter, Ina Ambiel, Sophie L. Winter, Liv Zimmermann, Tamara Naumoska, Felix Bubeck, Daniel Kirrmaier, Stephanie Ullrich, Isabel Barreto Miranda, Simon Anders, Dirk Grimm, Paul Schnitzler, Michael Knop, Hans-Georg Kräusslich, Viet Loan Dao Thi, Kathleen Börner andnPetr Chlanda |
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| 520 | |a Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot. | ||
| 650 | 4 | |a coronavirus | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a diagnostics | |
| 650 | 4 | |a high-throughput screening | |
| 650 | 4 | |a magnetic bead RNA purification | |
| 650 | 4 | |a pandemic | |
| 650 | 4 | |a RNA virus | |
| 650 | 4 | |a RT-LAMP | |
| 650 | 4 | |a RT-qPCR | |
| 650 | 4 | |a SARS-CoV-2 | |
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| 700 | 1 | |a Heuser, Anke-Mareil |e verfasserin |0 (DE-588)1072324881 |0 (DE-627)827147694 |0 (DE-576)433777478 |4 aut | |
| 700 | 1 | |a Glass, Bärbel |e verfasserin |0 (DE-588)102760031X |0 (DE-627)729455165 |0 (DE-576)373447884 |4 aut | |
| 700 | 1 | |a Meurer, Matthias |e verfasserin |0 (DE-588)1064186963 |0 (DE-627)813075610 |0 (DE-576)423831542 |4 aut | |
| 700 | 1 | |a Morales, Ivonne |e verfasserin |0 (DE-588)1012958795 |0 (DE-627)662778855 |0 (DE-576)346239923 |4 aut | |
| 700 | 1 | |a Schillak, Angelika |e verfasserin |0 (DE-588)1222452243 |0 (DE-627)174149737X |4 aut | |
| 700 | 1 | |a Freistaedter, Andrew |e verfasserin |0 (DE-588)1222680963 |0 (DE-627)1741842735 |4 aut | |
| 700 | 1 | |a Ambiel, Ina |e verfasserin |0 (DE-588)1026682304 |0 (DE-627)727057154 |0 (DE-576)37196881X |4 aut | |
| 700 | 1 | |a Winter, Sophie L. |d 1992- |e verfasserin |0 (DE-588)1222681641 |0 (DE-627)1741844622 |4 aut | |
| 700 | 1 | |a Zimmermann, Liv |e verfasserin |0 (DE-588)1222681927 |0 (DE-627)1741845564 |4 aut | |
| 700 | 1 | |a Naumoska, Tamara |e verfasserin |0 (DE-588)1222682060 |0 (DE-627)174184620X |4 aut | |
| 700 | 1 | |a Bubeck, Felix |e verfasserin |0 (DE-588)1172685525 |0 (DE-627)104131955X |0 (DE-576)514732695 |4 aut | |
| 700 | 1 | |a Kirrmaier, Daniel |e verfasserin |0 (DE-588)1064187080 |0 (DE-627)813075866 |0 (DE-576)423831496 |4 aut | |
| 700 | 1 | |a Ullrich, Stephanie |e verfasserin |0 (DE-588)1222683431 |0 (DE-627)1741854601 |4 aut | |
| 700 | 1 | |a Barreto Miranda, Isabel |d 1975- |e verfasserin |0 (DE-588)137337612 |0 (DE-627)59210530X |0 (DE-576)303193263 |4 aut | |
| 700 | 1 | |a Anders, Simon |e verfasserin |0 (DE-588)1184492964 |0 (DE-627)1663597936 |4 aut | |
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| 700 | 1 | |a Schnitzler, Paul |e verfasserin |0 (DE-588)1022905643 |0 (DE-627)717329798 |0 (DE-576)366155695 |4 aut | |
| 700 | 1 | |a Knop, Michael |e verfasserin |0 (DE-588)1064187552 |0 (DE-627)813076749 |0 (DE-576)423832107 |4 aut | |
| 700 | 1 | |a Kräusslich, Hans-Georg |d 1958- |e verfasserin |0 (DE-588)1027601782 |0 (DE-627)729459004 |0 (DE-576)373450524 |4 aut | |
| 700 | 1 | |a Dao Thi, Viet Loan |e verfasserin |0 (DE-588)1186079657 |0 (DE-627)1665348402 |4 aut | |
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| 700 | 1 | |a Chlanda, Petr |e verfasserin |0 (DE-588)141254823 |0 (DE-627)626165261 |0 (DE-576)323011152 |4 aut | |
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| 982 | |2 2013 |1 02 |x DE-16-250 |8 08 |s k |0 (DE-627)1495544966 |a Zentrum für Infektiologie | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 08 |s k |0 (DE-627)1416466967 |a Medizinische Fakultät Heidelberg | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 08 |s s |0 (DE-627)1410501914 |a Verfasser | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 08 |s s |a pos_21 | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 09 |s p |0 (DE-627)149383195X |a Knop, Michael | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 09 |s k |0 (DE-627)1416733221 |a Zentrum für Molekulare Biologie (ZMBH) | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 09 |s s |0 (DE-627)1410501914 |a Verfasser | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 09 |s s |a pos_22 | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 10 |s p |0 (DE-627)1443450340 |a Kräusslich, Hans-Georg | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 10 |s k |0 (DE-627)1495544966 |a Zentrum für Infektiologie | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 10 |s s |0 (DE-627)1410501914 |a Verfasser | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 10 |s s |a pos_23 | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 11 |s p |0 (DE-627)166562194X |a Dao Thi, Viet Loan | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 11 |s k |0 (DE-627)1495544966 |a Zentrum für Infektiologie | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 11 |s s |0 (DE-627)1410501914 |a Verfasser | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 11 |s s |a pos_24 | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 12 |s p |0 (DE-627)150312648X |a Börner, Kathleen | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 12 |s k |0 (DE-627)1495544966 |a Zentrum für Infektiologie | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 12 |s s |0 (DE-627)1410501914 |a Verfasser | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 12 |s s |a pos_25 | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 13 |s p |0 (DE-627)1741857848 |a Chlanda, Petr | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 13 |s k |0 (DE-627)1495544966 |a Zentrum für Infektiologie | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 13 |s s |0 (DE-627)1410501914 |a Verfasser | ||
| 982 | |2 2013 |1 02 |x DE-16-250 |8 13 |s s |a pos_26 | ||
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7 August 2020 |
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10.3390/v12080863 doi (DE-627)1741841836 (DE-599)KXP1741841836 (OCoLC)1341382828 DE-627 ger DE-627 rda eng Klein, Steffen 1989- verfasserin (DE-588)1209782782 (DE-627)1697757294 aut SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP Steffen Klein, Thorsten G. Müller, Dina Khalid, Vera Sonntag-Buck, Anke-Mareil Heuser, Bärbel Glass, Matthias Meurer, Ivonne Morales, Angelika Schillak, Andrew Freistaedter, Ina Ambiel, Sophie L. Winter, Liv Zimmermann, Tamara Naumoska, Felix Bubeck, Daniel Kirrmaier, Stephanie Ullrich, Isabel Barreto Miranda, Simon Anders, Dirk Grimm, Paul Schnitzler, Michael Knop, Hans-Georg Kräusslich, Viet Loan Dao Thi, Kathleen Börner andnPetr Chlanda 7 August 2020 13 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 03.12.2020 Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot. coronavirus COVID-19 diagnostics high-throughput screening magnetic bead RNA purification pandemic RNA virus RT-LAMP RT-qPCR SARS-CoV-2 Müller, Thorsten G. 1986- verfasserin (DE-588)1195239726 (DE-627)1677402016 aut Khalid, Dina 1980- verfasserin (DE-588)1151068179 (DE-627)1011257408 (DE-576)497384876 aut Sonntag-Buck, Vera verfasserin (DE-588)1222451107 (DE-627)1741493935 aut Heuser, Anke-Mareil verfasserin (DE-588)1072324881 (DE-627)827147694 (DE-576)433777478 aut Glass, Bärbel verfasserin (DE-588)102760031X (DE-627)729455165 (DE-576)373447884 aut Meurer, Matthias verfasserin (DE-588)1064186963 (DE-627)813075610 (DE-576)423831542 aut Morales, Ivonne verfasserin (DE-588)1012958795 (DE-627)662778855 (DE-576)346239923 aut Schillak, Angelika verfasserin (DE-588)1222452243 (DE-627)174149737X aut Freistaedter, Andrew verfasserin (DE-588)1222680963 (DE-627)1741842735 aut Ambiel, Ina verfasserin (DE-588)1026682304 (DE-627)727057154 (DE-576)37196881X aut Winter, Sophie L. 1992- verfasserin (DE-588)1222681641 (DE-627)1741844622 aut Zimmermann, Liv verfasserin (DE-588)1222681927 (DE-627)1741845564 aut Naumoska, Tamara verfasserin (DE-588)1222682060 (DE-627)174184620X aut Bubeck, Felix verfasserin (DE-588)1172685525 (DE-627)104131955X (DE-576)514732695 aut Kirrmaier, Daniel verfasserin (DE-588)1064187080 (DE-627)813075866 (DE-576)423831496 aut Ullrich, Stephanie verfasserin (DE-588)1222683431 (DE-627)1741854601 aut Barreto Miranda, Isabel 1975- verfasserin (DE-588)137337612 (DE-627)59210530X (DE-576)303193263 aut Anders, Simon verfasserin (DE-588)1184492964 (DE-627)1663597936 aut Grimm, Dirk verfasserin (DE-588)1016476671 (DE-627)70553376X (DE-576)352260645 aut Schnitzler, Paul verfasserin (DE-588)1022905643 (DE-627)717329798 (DE-576)366155695 aut Knop, Michael verfasserin (DE-588)1064187552 (DE-627)813076749 (DE-576)423832107 aut Kräusslich, Hans-Georg 1958- verfasserin (DE-588)1027601782 (DE-627)729459004 (DE-576)373450524 aut Dao Thi, Viet Loan verfasserin (DE-588)1186079657 (DE-627)1665348402 aut Börner, Kathleen verfasserin (DE-588)1030276382 (DE-627)734977727 (DE-576)378068806 aut Chlanda, Petr verfasserin (DE-588)141254823 (DE-627)626165261 (DE-576)323011152 aut Enthalten in Viruses Basel : MDPI, 2009 12(2020,8) Artikel-Nummer 863, 13 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:12 year:2020 number:8 extent:13 https://doi.org/10.3390/v12080863 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/12/8/863 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 8 13 12(2020,8) Artikel-Nummer 863, 13 Seiten 2013 01 DE-16-250 3816522882 00 --%%-- --%%-- --%%-- --%%-- l01 03-12-20 2013 02 DE-16-250 3816544339 00 --%%-- --%%-- --%%-- --%%-- l02 03-12-20 2013 01 DE-16-250 00 s hd2020 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_26 2013 01 DE-16-250 03 s s_13 2013 01 DE-16-250 04 p (DE-627)1697759580 Klein, Steffen 2013 01 DE-16-250 04 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)167740227X Müller, Thorsten G. 2013 01 DE-16-250 05 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1741492394 Khalid, Dina 2013 01 DE-16-250 06 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1741494176 Sonntag-Buck, Vera 2013 01 DE-16-250 07 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1503777642 Heuser, Anke-Mareil 2013 01 DE-16-250 08 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1443447773 Glass, Bärbel 2013 01 DE-16-250 09 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 2013 01 DE-16-250 10 p (DE-627)1493831402 Meurer, Matthias 2013 01 DE-16-250 10 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 10 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 10 s pos_7 2013 01 DE-16-250 11 p (DE-627)1503648370 Morales, Ivonne 2013 01 DE-16-250 11 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 11 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 11 s pos_8 2013 01 DE-16-250 12 p (DE-627)1741497779 Schillack, Angelika 2013 01 DE-16-250 12 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 12 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 12 s pos_9 2013 01 DE-16-250 13 p (DE-627)174184309X Freistaedter, Andrew 2013 01 DE-16-250 13 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 13 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 13 s pos_10 2013 01 DE-16-250 14 p (DE-627)1441969489 Ambiel, Ina 2013 01 DE-16-250 14 k (DE-627)1495544966 Zentrum für 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| spelling |
10.3390/v12080863 doi (DE-627)1741841836 (DE-599)KXP1741841836 (OCoLC)1341382828 DE-627 ger DE-627 rda eng Klein, Steffen 1989- verfasserin (DE-588)1209782782 (DE-627)1697757294 aut SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP Steffen Klein, Thorsten G. Müller, Dina Khalid, Vera Sonntag-Buck, Anke-Mareil Heuser, Bärbel Glass, Matthias Meurer, Ivonne Morales, Angelika Schillak, Andrew Freistaedter, Ina Ambiel, Sophie L. Winter, Liv Zimmermann, Tamara Naumoska, Felix Bubeck, Daniel Kirrmaier, Stephanie Ullrich, Isabel Barreto Miranda, Simon Anders, Dirk Grimm, Paul Schnitzler, Michael Knop, Hans-Georg Kräusslich, Viet Loan Dao Thi, Kathleen Börner andnPetr Chlanda 7 August 2020 13 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 03.12.2020 Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot. coronavirus COVID-19 diagnostics high-throughput screening magnetic bead RNA purification pandemic RNA virus RT-LAMP RT-qPCR SARS-CoV-2 Müller, Thorsten G. 1986- verfasserin (DE-588)1195239726 (DE-627)1677402016 aut Khalid, Dina 1980- verfasserin (DE-588)1151068179 (DE-627)1011257408 (DE-576)497384876 aut Sonntag-Buck, Vera verfasserin (DE-588)1222451107 (DE-627)1741493935 aut Heuser, Anke-Mareil verfasserin (DE-588)1072324881 (DE-627)827147694 (DE-576)433777478 aut Glass, Bärbel verfasserin (DE-588)102760031X (DE-627)729455165 (DE-576)373447884 aut Meurer, Matthias verfasserin (DE-588)1064186963 (DE-627)813075610 (DE-576)423831542 aut Morales, Ivonne verfasserin (DE-588)1012958795 (DE-627)662778855 (DE-576)346239923 aut Schillak, Angelika verfasserin (DE-588)1222452243 (DE-627)174149737X aut Freistaedter, Andrew verfasserin (DE-588)1222680963 (DE-627)1741842735 aut Ambiel, Ina verfasserin (DE-588)1026682304 (DE-627)727057154 (DE-576)37196881X aut Winter, Sophie L. 1992- verfasserin (DE-588)1222681641 (DE-627)1741844622 aut Zimmermann, Liv verfasserin (DE-588)1222681927 (DE-627)1741845564 aut Naumoska, Tamara verfasserin (DE-588)1222682060 (DE-627)174184620X aut Bubeck, Felix verfasserin (DE-588)1172685525 (DE-627)104131955X (DE-576)514732695 aut Kirrmaier, Daniel verfasserin (DE-588)1064187080 (DE-627)813075866 (DE-576)423831496 aut Ullrich, Stephanie verfasserin (DE-588)1222683431 (DE-627)1741854601 aut Barreto Miranda, Isabel 1975- verfasserin (DE-588)137337612 (DE-627)59210530X (DE-576)303193263 aut Anders, Simon verfasserin (DE-588)1184492964 (DE-627)1663597936 aut Grimm, Dirk verfasserin (DE-588)1016476671 (DE-627)70553376X (DE-576)352260645 aut Schnitzler, Paul verfasserin (DE-588)1022905643 (DE-627)717329798 (DE-576)366155695 aut Knop, Michael verfasserin (DE-588)1064187552 (DE-627)813076749 (DE-576)423832107 aut Kräusslich, Hans-Georg 1958- verfasserin (DE-588)1027601782 (DE-627)729459004 (DE-576)373450524 aut Dao Thi, Viet Loan verfasserin (DE-588)1186079657 (DE-627)1665348402 aut Börner, Kathleen verfasserin (DE-588)1030276382 (DE-627)734977727 (DE-576)378068806 aut Chlanda, Petr verfasserin (DE-588)141254823 (DE-627)626165261 (DE-576)323011152 aut Enthalten in Viruses Basel : MDPI, 2009 12(2020,8) Artikel-Nummer 863, 13 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:12 year:2020 number:8 extent:13 https://doi.org/10.3390/v12080863 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/12/8/863 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 8 13 12(2020,8) Artikel-Nummer 863, 13 Seiten 2013 01 DE-16-250 3816522882 00 --%%-- --%%-- --%%-- --%%-- l01 03-12-20 2013 02 DE-16-250 3816544339 00 --%%-- --%%-- --%%-- --%%-- l02 03-12-20 2013 01 DE-16-250 00 s hd2020 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_26 2013 01 DE-16-250 03 s s_13 2013 01 DE-16-250 04 p (DE-627)1697759580 Klein, Steffen 2013 01 DE-16-250 04 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)167740227X Müller, Thorsten G. 2013 01 DE-16-250 05 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1741492394 Khalid, Dina 2013 01 DE-16-250 06 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1741494176 Sonntag-Buck, Vera 2013 01 DE-16-250 07 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1503777642 Heuser, Anke-Mareil 2013 01 DE-16-250 08 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1443447773 Glass, Bärbel 2013 01 DE-16-250 09 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 2013 01 DE-16-250 10 p (DE-627)1493831402 Meurer, Matthias 2013 01 DE-16-250 10 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 10 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 10 s pos_7 2013 01 DE-16-250 11 p (DE-627)1503648370 Morales, Ivonne 2013 01 DE-16-250 11 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 11 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 11 s pos_8 2013 01 DE-16-250 12 p (DE-627)1741497779 Schillack, Angelika 2013 01 DE-16-250 12 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 12 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 12 s pos_9 2013 01 DE-16-250 13 p (DE-627)174184309X Freistaedter, Andrew 2013 01 DE-16-250 13 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 13 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 13 s pos_10 2013 01 DE-16-250 14 p (DE-627)1441969489 Ambiel, Ina 2013 01 DE-16-250 14 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 14 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 14 s pos_11 2013 01 DE-16-250 15 p (DE-627)1741844908 Winter, Sophie L. 2013 01 DE-16-250 15 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 15 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 15 s pos_12 2013 01 DE-16-250 16 p (DE-627)1741845823 Zimmermann, Liv 2013 01 DE-16-250 16 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 16 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 16 s pos_13 2013 01 DE-16-250 17 p (DE-627)1741846579 Naumoska, Tamara 2013 01 DE-16-250 17 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 17 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 17 s pos_14 2013 01 DE-16-250 18 p (DE-627)1584732598 Bubeck, Felix 2013 01 DE-16-250 18 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 18 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 18 s pos_15 2013 01 DE-16-250 19 p (DE-627)1493831461 Kirrmaier, Daniel 2013 01 DE-16-250 19 k (DE-627)1416535500 Fakultät für Biowissenschaften 2013 01 DE-16-250 19 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 19 s pos_16 2013 02 DE-16-250 00 s hd2020 2013 02 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 02 DE-16-250 02 s per_26 2013 02 DE-16-250 03 s s_13 2013 02 DE-16-250 04 p (DE-627)1741854938 Ullrich, Stephanie 2013 02 DE-16-250 04 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 04 s pos_17 2013 02 DE-16-250 05 p (DE-627)1702121828 Barreto Miranda, Isabel 2013 02 DE-16-250 05 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 05 s pos_18 2013 02 DE-16-250 06 p (DE-627)1663598371 Anders, Simon 2013 02 DE-16-250 06 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 02 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 06 s pos_19 2013 02 DE-16-250 07 p (DE-627)1466739088 Grimm, Dirk 2013 02 DE-16-250 07 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 07 s pos_20 2013 02 DE-16-250 08 p (DE-627)1436156459 Schnitzler, Paul 2013 02 DE-16-250 08 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 08 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 02 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 08 s pos_21 2013 02 DE-16-250 09 p (DE-627)149383195X Knop, Michael 2013 02 DE-16-250 09 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 02 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 09 s pos_22 2013 02 DE-16-250 10 p (DE-627)1443450340 Kräusslich, Hans-Georg 2013 02 DE-16-250 10 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 10 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 10 s pos_23 2013 02 DE-16-250 11 p (DE-627)166562194X Dao Thi, Viet Loan 2013 02 DE-16-250 11 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 11 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 11 s pos_24 2013 02 DE-16-250 12 p (DE-627)150312648X Börner, Kathleen 2013 02 DE-16-250 12 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 12 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 12 s pos_25 2013 02 DE-16-250 13 p (DE-627)1741857848 Chlanda, Petr 2013 02 DE-16-250 13 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 13 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 13 s pos_26 |
| allfields_unstemmed |
10.3390/v12080863 doi (DE-627)1741841836 (DE-599)KXP1741841836 (OCoLC)1341382828 DE-627 ger DE-627 rda eng Klein, Steffen 1989- verfasserin (DE-588)1209782782 (DE-627)1697757294 aut SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP Steffen Klein, Thorsten G. Müller, Dina Khalid, Vera Sonntag-Buck, Anke-Mareil Heuser, Bärbel Glass, Matthias Meurer, Ivonne Morales, Angelika Schillak, Andrew Freistaedter, Ina Ambiel, Sophie L. Winter, Liv Zimmermann, Tamara Naumoska, Felix Bubeck, Daniel Kirrmaier, Stephanie Ullrich, Isabel Barreto Miranda, Simon Anders, Dirk Grimm, Paul Schnitzler, Michael Knop, Hans-Georg Kräusslich, Viet Loan Dao Thi, Kathleen Börner andnPetr Chlanda 7 August 2020 13 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 03.12.2020 Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot. coronavirus COVID-19 diagnostics high-throughput screening magnetic bead RNA purification pandemic RNA virus RT-LAMP RT-qPCR SARS-CoV-2 Müller, Thorsten G. 1986- verfasserin (DE-588)1195239726 (DE-627)1677402016 aut Khalid, Dina 1980- verfasserin (DE-588)1151068179 (DE-627)1011257408 (DE-576)497384876 aut Sonntag-Buck, Vera verfasserin (DE-588)1222451107 (DE-627)1741493935 aut Heuser, Anke-Mareil verfasserin (DE-588)1072324881 (DE-627)827147694 (DE-576)433777478 aut Glass, Bärbel verfasserin (DE-588)102760031X (DE-627)729455165 (DE-576)373447884 aut Meurer, Matthias verfasserin (DE-588)1064186963 (DE-627)813075610 (DE-576)423831542 aut Morales, Ivonne verfasserin (DE-588)1012958795 (DE-627)662778855 (DE-576)346239923 aut Schillak, Angelika verfasserin (DE-588)1222452243 (DE-627)174149737X aut Freistaedter, Andrew verfasserin (DE-588)1222680963 (DE-627)1741842735 aut Ambiel, Ina verfasserin (DE-588)1026682304 (DE-627)727057154 (DE-576)37196881X aut Winter, Sophie L. 1992- verfasserin (DE-588)1222681641 (DE-627)1741844622 aut Zimmermann, Liv verfasserin (DE-588)1222681927 (DE-627)1741845564 aut Naumoska, Tamara verfasserin (DE-588)1222682060 (DE-627)174184620X aut Bubeck, Felix verfasserin (DE-588)1172685525 (DE-627)104131955X (DE-576)514732695 aut Kirrmaier, Daniel verfasserin (DE-588)1064187080 (DE-627)813075866 (DE-576)423831496 aut Ullrich, Stephanie verfasserin (DE-588)1222683431 (DE-627)1741854601 aut Barreto Miranda, Isabel 1975- verfasserin (DE-588)137337612 (DE-627)59210530X (DE-576)303193263 aut Anders, Simon verfasserin (DE-588)1184492964 (DE-627)1663597936 aut Grimm, Dirk verfasserin (DE-588)1016476671 (DE-627)70553376X (DE-576)352260645 aut Schnitzler, Paul verfasserin (DE-588)1022905643 (DE-627)717329798 (DE-576)366155695 aut Knop, Michael verfasserin (DE-588)1064187552 (DE-627)813076749 (DE-576)423832107 aut Kräusslich, Hans-Georg 1958- verfasserin (DE-588)1027601782 (DE-627)729459004 (DE-576)373450524 aut Dao Thi, Viet Loan verfasserin (DE-588)1186079657 (DE-627)1665348402 aut Börner, Kathleen verfasserin (DE-588)1030276382 (DE-627)734977727 (DE-576)378068806 aut Chlanda, Petr verfasserin (DE-588)141254823 (DE-627)626165261 (DE-576)323011152 aut Enthalten in Viruses Basel : MDPI, 2009 12(2020,8) Artikel-Nummer 863, 13 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:12 year:2020 number:8 extent:13 https://doi.org/10.3390/v12080863 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/12/8/863 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 8 13 12(2020,8) Artikel-Nummer 863, 13 Seiten 2013 01 DE-16-250 3816522882 00 --%%-- --%%-- --%%-- --%%-- l01 03-12-20 2013 02 DE-16-250 3816544339 00 --%%-- --%%-- --%%-- --%%-- l02 03-12-20 2013 01 DE-16-250 00 s hd2020 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_26 2013 01 DE-16-250 03 s s_13 2013 01 DE-16-250 04 p (DE-627)1697759580 Klein, Steffen 2013 01 DE-16-250 04 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)167740227X Müller, Thorsten G. 2013 01 DE-16-250 05 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1741492394 Khalid, Dina 2013 01 DE-16-250 06 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1741494176 Sonntag-Buck, Vera 2013 01 DE-16-250 07 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1503777642 Heuser, Anke-Mareil 2013 01 DE-16-250 08 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1443447773 Glass, Bärbel 2013 01 DE-16-250 09 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 2013 01 DE-16-250 10 p (DE-627)1493831402 Meurer, Matthias 2013 01 DE-16-250 10 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 10 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 10 s pos_7 2013 01 DE-16-250 11 p (DE-627)1503648370 Morales, Ivonne 2013 01 DE-16-250 11 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 11 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 11 s pos_8 2013 01 DE-16-250 12 p (DE-627)1741497779 Schillack, Angelika 2013 01 DE-16-250 12 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 12 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 12 s pos_9 2013 01 DE-16-250 13 p (DE-627)174184309X Freistaedter, Andrew 2013 01 DE-16-250 13 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 13 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 13 s pos_10 2013 01 DE-16-250 14 p (DE-627)1441969489 Ambiel, Ina 2013 01 DE-16-250 14 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 14 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 14 s pos_11 2013 01 DE-16-250 15 p (DE-627)1741844908 Winter, Sophie L. 2013 01 DE-16-250 15 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 15 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 15 s pos_12 2013 01 DE-16-250 16 p (DE-627)1741845823 Zimmermann, Liv 2013 01 DE-16-250 16 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 16 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 16 s pos_13 2013 01 DE-16-250 17 p (DE-627)1741846579 Naumoska, Tamara 2013 01 DE-16-250 17 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 17 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 17 s pos_14 2013 01 DE-16-250 18 p (DE-627)1584732598 Bubeck, Felix 2013 01 DE-16-250 18 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 18 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 18 s pos_15 2013 01 DE-16-250 19 p (DE-627)1493831461 Kirrmaier, Daniel 2013 01 DE-16-250 19 k (DE-627)1416535500 Fakultät für Biowissenschaften 2013 01 DE-16-250 19 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 19 s pos_16 2013 02 DE-16-250 00 s hd2020 2013 02 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 02 DE-16-250 02 s per_26 2013 02 DE-16-250 03 s s_13 2013 02 DE-16-250 04 p (DE-627)1741854938 Ullrich, Stephanie 2013 02 DE-16-250 04 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 04 s pos_17 2013 02 DE-16-250 05 p (DE-627)1702121828 Barreto Miranda, Isabel 2013 02 DE-16-250 05 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 05 s pos_18 2013 02 DE-16-250 06 p (DE-627)1663598371 Anders, Simon 2013 02 DE-16-250 06 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 02 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 06 s pos_19 2013 02 DE-16-250 07 p (DE-627)1466739088 Grimm, Dirk 2013 02 DE-16-250 07 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 07 s pos_20 2013 02 DE-16-250 08 p (DE-627)1436156459 Schnitzler, Paul 2013 02 DE-16-250 08 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 08 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 02 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 08 s pos_21 2013 02 DE-16-250 09 p (DE-627)149383195X Knop, Michael 2013 02 DE-16-250 09 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 02 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 09 s pos_22 2013 02 DE-16-250 10 p (DE-627)1443450340 Kräusslich, Hans-Georg 2013 02 DE-16-250 10 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 10 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 10 s pos_23 2013 02 DE-16-250 11 p (DE-627)166562194X Dao Thi, Viet Loan 2013 02 DE-16-250 11 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 11 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 11 s pos_24 2013 02 DE-16-250 12 p (DE-627)150312648X Börner, Kathleen 2013 02 DE-16-250 12 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 12 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 12 s pos_25 2013 02 DE-16-250 13 p (DE-627)1741857848 Chlanda, Petr 2013 02 DE-16-250 13 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 13 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 13 s pos_26 |
| allfieldsGer |
10.3390/v12080863 doi (DE-627)1741841836 (DE-599)KXP1741841836 (OCoLC)1341382828 DE-627 ger DE-627 rda eng Klein, Steffen 1989- verfasserin (DE-588)1209782782 (DE-627)1697757294 aut SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP Steffen Klein, Thorsten G. Müller, Dina Khalid, Vera Sonntag-Buck, Anke-Mareil Heuser, Bärbel Glass, Matthias Meurer, Ivonne Morales, Angelika Schillak, Andrew Freistaedter, Ina Ambiel, Sophie L. Winter, Liv Zimmermann, Tamara Naumoska, Felix Bubeck, Daniel Kirrmaier, Stephanie Ullrich, Isabel Barreto Miranda, Simon Anders, Dirk Grimm, Paul Schnitzler, Michael Knop, Hans-Georg Kräusslich, Viet Loan Dao Thi, Kathleen Börner andnPetr Chlanda 7 August 2020 13 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 03.12.2020 Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot. coronavirus COVID-19 diagnostics high-throughput screening magnetic bead RNA purification pandemic RNA virus RT-LAMP RT-qPCR SARS-CoV-2 Müller, Thorsten G. 1986- verfasserin (DE-588)1195239726 (DE-627)1677402016 aut Khalid, Dina 1980- verfasserin (DE-588)1151068179 (DE-627)1011257408 (DE-576)497384876 aut Sonntag-Buck, Vera verfasserin (DE-588)1222451107 (DE-627)1741493935 aut Heuser, Anke-Mareil verfasserin (DE-588)1072324881 (DE-627)827147694 (DE-576)433777478 aut Glass, Bärbel verfasserin (DE-588)102760031X (DE-627)729455165 (DE-576)373447884 aut Meurer, Matthias verfasserin (DE-588)1064186963 (DE-627)813075610 (DE-576)423831542 aut Morales, Ivonne verfasserin (DE-588)1012958795 (DE-627)662778855 (DE-576)346239923 aut Schillak, Angelika verfasserin (DE-588)1222452243 (DE-627)174149737X aut Freistaedter, Andrew verfasserin (DE-588)1222680963 (DE-627)1741842735 aut Ambiel, Ina verfasserin (DE-588)1026682304 (DE-627)727057154 (DE-576)37196881X aut Winter, Sophie L. 1992- verfasserin (DE-588)1222681641 (DE-627)1741844622 aut Zimmermann, Liv verfasserin (DE-588)1222681927 (DE-627)1741845564 aut Naumoska, Tamara verfasserin (DE-588)1222682060 (DE-627)174184620X aut Bubeck, Felix verfasserin (DE-588)1172685525 (DE-627)104131955X (DE-576)514732695 aut Kirrmaier, Daniel verfasserin (DE-588)1064187080 (DE-627)813075866 (DE-576)423831496 aut Ullrich, Stephanie verfasserin (DE-588)1222683431 (DE-627)1741854601 aut Barreto Miranda, Isabel 1975- verfasserin (DE-588)137337612 (DE-627)59210530X (DE-576)303193263 aut Anders, Simon verfasserin (DE-588)1184492964 (DE-627)1663597936 aut Grimm, Dirk verfasserin (DE-588)1016476671 (DE-627)70553376X (DE-576)352260645 aut Schnitzler, Paul verfasserin (DE-588)1022905643 (DE-627)717329798 (DE-576)366155695 aut Knop, Michael verfasserin (DE-588)1064187552 (DE-627)813076749 (DE-576)423832107 aut Kräusslich, Hans-Georg 1958- verfasserin (DE-588)1027601782 (DE-627)729459004 (DE-576)373450524 aut Dao Thi, Viet Loan verfasserin (DE-588)1186079657 (DE-627)1665348402 aut Börner, Kathleen verfasserin (DE-588)1030276382 (DE-627)734977727 (DE-576)378068806 aut Chlanda, Petr verfasserin (DE-588)141254823 (DE-627)626165261 (DE-576)323011152 aut Enthalten in Viruses Basel : MDPI, 2009 12(2020,8) Artikel-Nummer 863, 13 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:12 year:2020 number:8 extent:13 https://doi.org/10.3390/v12080863 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/12/8/863 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 8 13 12(2020,8) Artikel-Nummer 863, 13 Seiten 2013 01 DE-16-250 3816522882 00 --%%-- --%%-- --%%-- --%%-- l01 03-12-20 2013 02 DE-16-250 3816544339 00 --%%-- --%%-- --%%-- --%%-- l02 03-12-20 2013 01 DE-16-250 00 s hd2020 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_26 2013 01 DE-16-250 03 s s_13 2013 01 DE-16-250 04 p (DE-627)1697759580 Klein, Steffen 2013 01 DE-16-250 04 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)167740227X Müller, Thorsten G. 2013 01 DE-16-250 05 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1741492394 Khalid, Dina 2013 01 DE-16-250 06 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1741494176 Sonntag-Buck, Vera 2013 01 DE-16-250 07 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1503777642 Heuser, Anke-Mareil 2013 01 DE-16-250 08 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1443447773 Glass, Bärbel 2013 01 DE-16-250 09 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 2013 01 DE-16-250 10 p (DE-627)1493831402 Meurer, Matthias 2013 01 DE-16-250 10 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 10 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 10 s pos_7 2013 01 DE-16-250 11 p (DE-627)1503648370 Morales, Ivonne 2013 01 DE-16-250 11 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 11 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 11 s pos_8 2013 01 DE-16-250 12 p (DE-627)1741497779 Schillack, Angelika 2013 01 DE-16-250 12 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 12 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 12 s pos_9 2013 01 DE-16-250 13 p (DE-627)174184309X Freistaedter, Andrew 2013 01 DE-16-250 13 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 13 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 13 s pos_10 2013 01 DE-16-250 14 p (DE-627)1441969489 Ambiel, Ina 2013 01 DE-16-250 14 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 14 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 14 s pos_11 2013 01 DE-16-250 15 p (DE-627)1741844908 Winter, Sophie L. 2013 01 DE-16-250 15 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 15 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 15 s pos_12 2013 01 DE-16-250 16 p (DE-627)1741845823 Zimmermann, Liv 2013 01 DE-16-250 16 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 16 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 16 s pos_13 2013 01 DE-16-250 17 p (DE-627)1741846579 Naumoska, Tamara 2013 01 DE-16-250 17 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 17 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 17 s pos_14 2013 01 DE-16-250 18 p (DE-627)1584732598 Bubeck, Felix 2013 01 DE-16-250 18 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 18 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 18 s pos_15 2013 01 DE-16-250 19 p (DE-627)1493831461 Kirrmaier, Daniel 2013 01 DE-16-250 19 k (DE-627)1416535500 Fakultät für Biowissenschaften 2013 01 DE-16-250 19 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 19 s pos_16 2013 02 DE-16-250 00 s hd2020 2013 02 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 02 DE-16-250 02 s per_26 2013 02 DE-16-250 03 s s_13 2013 02 DE-16-250 04 p (DE-627)1741854938 Ullrich, Stephanie 2013 02 DE-16-250 04 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 04 s pos_17 2013 02 DE-16-250 05 p (DE-627)1702121828 Barreto Miranda, Isabel 2013 02 DE-16-250 05 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 05 s pos_18 2013 02 DE-16-250 06 p (DE-627)1663598371 Anders, Simon 2013 02 DE-16-250 06 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 02 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 06 s pos_19 2013 02 DE-16-250 07 p (DE-627)1466739088 Grimm, Dirk 2013 02 DE-16-250 07 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 07 s pos_20 2013 02 DE-16-250 08 p (DE-627)1436156459 Schnitzler, Paul 2013 02 DE-16-250 08 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 08 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 02 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 08 s pos_21 2013 02 DE-16-250 09 p (DE-627)149383195X Knop, Michael 2013 02 DE-16-250 09 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 02 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 09 s pos_22 2013 02 DE-16-250 10 p (DE-627)1443450340 Kräusslich, Hans-Georg 2013 02 DE-16-250 10 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 10 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 10 s pos_23 2013 02 DE-16-250 11 p (DE-627)166562194X Dao Thi, Viet Loan 2013 02 DE-16-250 11 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 11 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 11 s pos_24 2013 02 DE-16-250 12 p (DE-627)150312648X Börner, Kathleen 2013 02 DE-16-250 12 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 12 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 12 s pos_25 2013 02 DE-16-250 13 p (DE-627)1741857848 Chlanda, Petr 2013 02 DE-16-250 13 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 13 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 13 s pos_26 |
| allfieldsSound |
10.3390/v12080863 doi (DE-627)1741841836 (DE-599)KXP1741841836 (OCoLC)1341382828 DE-627 ger DE-627 rda eng Klein, Steffen 1989- verfasserin (DE-588)1209782782 (DE-627)1697757294 aut SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP Steffen Klein, Thorsten G. Müller, Dina Khalid, Vera Sonntag-Buck, Anke-Mareil Heuser, Bärbel Glass, Matthias Meurer, Ivonne Morales, Angelika Schillak, Andrew Freistaedter, Ina Ambiel, Sophie L. Winter, Liv Zimmermann, Tamara Naumoska, Felix Bubeck, Daniel Kirrmaier, Stephanie Ullrich, Isabel Barreto Miranda, Simon Anders, Dirk Grimm, Paul Schnitzler, Michael Knop, Hans-Georg Kräusslich, Viet Loan Dao Thi, Kathleen Börner andnPetr Chlanda 7 August 2020 13 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 03.12.2020 Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot. coronavirus COVID-19 diagnostics high-throughput screening magnetic bead RNA purification pandemic RNA virus RT-LAMP RT-qPCR SARS-CoV-2 Müller, Thorsten G. 1986- verfasserin (DE-588)1195239726 (DE-627)1677402016 aut Khalid, Dina 1980- verfasserin (DE-588)1151068179 (DE-627)1011257408 (DE-576)497384876 aut Sonntag-Buck, Vera verfasserin (DE-588)1222451107 (DE-627)1741493935 aut Heuser, Anke-Mareil verfasserin (DE-588)1072324881 (DE-627)827147694 (DE-576)433777478 aut Glass, Bärbel verfasserin (DE-588)102760031X (DE-627)729455165 (DE-576)373447884 aut Meurer, Matthias verfasserin (DE-588)1064186963 (DE-627)813075610 (DE-576)423831542 aut Morales, Ivonne verfasserin (DE-588)1012958795 (DE-627)662778855 (DE-576)346239923 aut Schillak, Angelika verfasserin (DE-588)1222452243 (DE-627)174149737X aut Freistaedter, Andrew verfasserin (DE-588)1222680963 (DE-627)1741842735 aut Ambiel, Ina verfasserin (DE-588)1026682304 (DE-627)727057154 (DE-576)37196881X aut Winter, Sophie L. 1992- verfasserin (DE-588)1222681641 (DE-627)1741844622 aut Zimmermann, Liv verfasserin (DE-588)1222681927 (DE-627)1741845564 aut Naumoska, Tamara verfasserin (DE-588)1222682060 (DE-627)174184620X aut Bubeck, Felix verfasserin (DE-588)1172685525 (DE-627)104131955X (DE-576)514732695 aut Kirrmaier, Daniel verfasserin (DE-588)1064187080 (DE-627)813075866 (DE-576)423831496 aut Ullrich, Stephanie verfasserin (DE-588)1222683431 (DE-627)1741854601 aut Barreto Miranda, Isabel 1975- verfasserin (DE-588)137337612 (DE-627)59210530X (DE-576)303193263 aut Anders, Simon verfasserin (DE-588)1184492964 (DE-627)1663597936 aut Grimm, Dirk verfasserin (DE-588)1016476671 (DE-627)70553376X (DE-576)352260645 aut Schnitzler, Paul verfasserin (DE-588)1022905643 (DE-627)717329798 (DE-576)366155695 aut Knop, Michael verfasserin (DE-588)1064187552 (DE-627)813076749 (DE-576)423832107 aut Kräusslich, Hans-Georg 1958- verfasserin (DE-588)1027601782 (DE-627)729459004 (DE-576)373450524 aut Dao Thi, Viet Loan verfasserin (DE-588)1186079657 (DE-627)1665348402 aut Börner, Kathleen verfasserin (DE-588)1030276382 (DE-627)734977727 (DE-576)378068806 aut Chlanda, Petr verfasserin (DE-588)141254823 (DE-627)626165261 (DE-576)323011152 aut Enthalten in Viruses Basel : MDPI, 2009 12(2020,8) Artikel-Nummer 863, 13 Seiten Online-Ressource (DE-627)609775871 (DE-600)2516098-9 (DE-576)311326080 1999-4915 nnns volume:12 year:2020 number:8 extent:13 https://doi.org/10.3390/v12080863 Verlag Resolving-System lizenzpflichtig Volltext https://www.mdpi.com/1999-4915/12/8/863 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 8 13 12(2020,8) Artikel-Nummer 863, 13 Seiten 2013 01 DE-16-250 3816522882 00 --%%-- --%%-- --%%-- --%%-- l01 03-12-20 2013 02 DE-16-250 3816544339 00 --%%-- --%%-- --%%-- --%%-- l02 03-12-20 2013 01 DE-16-250 00 s hd2020 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_26 2013 01 DE-16-250 03 s s_13 2013 01 DE-16-250 04 p (DE-627)1697759580 Klein, Steffen 2013 01 DE-16-250 04 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)167740227X Müller, Thorsten G. 2013 01 DE-16-250 05 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1741492394 Khalid, Dina 2013 01 DE-16-250 06 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1741494176 Sonntag-Buck, Vera 2013 01 DE-16-250 07 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1503777642 Heuser, Anke-Mareil 2013 01 DE-16-250 08 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1443447773 Glass, Bärbel 2013 01 DE-16-250 09 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 2013 01 DE-16-250 10 p (DE-627)1493831402 Meurer, Matthias 2013 01 DE-16-250 10 k (DE-627)1416733221 Zentrum für Molekulare Biologie (ZMBH) 2013 01 DE-16-250 10 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 10 s pos_7 2013 01 DE-16-250 11 p (DE-627)1503648370 Morales, Ivonne 2013 01 DE-16-250 11 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 11 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 11 s pos_8 2013 01 DE-16-250 12 p (DE-627)1741497779 Schillack, Angelika 2013 01 DE-16-250 12 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 12 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 12 s pos_9 2013 01 DE-16-250 13 p (DE-627)174184309X Freistaedter, Andrew 2013 01 DE-16-250 13 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 13 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 13 s pos_10 2013 01 DE-16-250 14 p (DE-627)1441969489 Ambiel, Ina 2013 01 DE-16-250 14 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 14 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 14 s pos_11 2013 01 DE-16-250 15 p (DE-627)1741844908 Winter, Sophie L. 2013 01 DE-16-250 15 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 15 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 15 s pos_12 2013 01 DE-16-250 16 p (DE-627)1741845823 Zimmermann, Liv 2013 01 DE-16-250 16 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 16 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 16 s pos_13 2013 01 DE-16-250 17 p (DE-627)1741846579 Naumoska, Tamara 2013 01 DE-16-250 17 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 17 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 17 s pos_14 2013 01 DE-16-250 18 p (DE-627)1584732598 Bubeck, Felix 2013 01 DE-16-250 18 k (DE-627)1495544966 Zentrum für Infektiologie 2013 01 DE-16-250 18 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 18 s pos_15 2013 01 DE-16-250 19 p (DE-627)1493831461 Kirrmaier, Daniel 2013 01 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(DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 11 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 11 s pos_24 2013 02 DE-16-250 12 p (DE-627)150312648X Börner, Kathleen 2013 02 DE-16-250 12 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 12 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 12 s pos_25 2013 02 DE-16-250 13 p (DE-627)1741857848 Chlanda, Petr 2013 02 DE-16-250 13 k (DE-627)1495544966 Zentrum für Infektiologie 2013 02 DE-16-250 13 s (DE-627)1410501914 Verfasser 2013 02 DE-16-250 13 s pos_26 |
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English |
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Enthalten in Viruses 12(2020,8) Artikel-Nummer 863, 13 Seiten volume:12 year:2020 number:8 extent:13 |
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Enthalten in Viruses 12(2020,8) Artikel-Nummer 863, 13 Seiten volume:12 year:2020 number:8 extent:13 |
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Klein, Steffen @@aut@@ Müller, Thorsten G. @@aut@@ Khalid, Dina @@aut@@ Sonntag-Buck, Vera @@aut@@ Heuser, Anke-Mareil @@aut@@ Glass, Bärbel @@aut@@ Meurer, Matthias @@aut@@ Morales, Ivonne @@aut@@ Schillak, Angelika @@aut@@ Freistaedter, Andrew @@aut@@ Ambiel, Ina @@aut@@ Winter, Sophie L. @@aut@@ Zimmermann, Liv @@aut@@ Naumoska, Tamara @@aut@@ Bubeck, Felix @@aut@@ Kirrmaier, Daniel @@aut@@ Ullrich, Stephanie @@aut@@ Barreto Miranda, Isabel @@aut@@ Anders, Simon @@aut@@ Grimm, Dirk @@aut@@ Schnitzler, Paul @@aut@@ Knop, Michael @@aut@@ Kräusslich, Hans-Georg @@aut@@ Dao Thi, Viet Loan @@aut@@ Börner, Kathleen @@aut@@ Chlanda, Petr @@aut@@ |
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2020-01-01T00:00:00Z |
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609775871 |
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1741841836 |
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englisch |
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Winter, Liv Zimmermann, Tamara Naumoska, Felix Bubeck, Daniel Kirrmaier, Stephanie Ullrich, Isabel Barreto Miranda, Simon Anders, Dirk Grimm, Paul Schnitzler, Michael Knop, Hans-Georg Kräusslich, Viet Loan Dao Thi, Kathleen Börner andnPetr Chlanda</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">7 August 2020</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">13</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Gesehen am 03.12.2020</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). 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Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. 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code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">13</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">13</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">13</subfield><subfield code="s">s</subfield><subfield code="a">pos_10</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">14</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1441969489</subfield><subfield code="a">Ambiel, Ina</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">14</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">14</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">14</subfield><subfield code="s">s</subfield><subfield code="a">pos_11</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">15</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1741844908</subfield><subfield code="a">Winter, Sophie L.</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">15</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">15</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">15</subfield><subfield code="s">s</subfield><subfield code="a">pos_12</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">16</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1741845823</subfield><subfield code="a">Zimmermann, Liv</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">16</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">16</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">16</subfield><subfield code="s">s</subfield><subfield code="a">pos_13</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">17</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1741846579</subfield><subfield code="a">Naumoska, Tamara</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">17</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">17</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">17</subfield><subfield code="s">s</subfield><subfield code="a">pos_14</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">18</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1584732598</subfield><subfield code="a">Bubeck, Felix</subfield></datafield><datafield tag="982" 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code="x">DE-16-250</subfield><subfield code="8">19</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1493831461</subfield><subfield code="a">Kirrmaier, Daniel</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">19</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1416535500</subfield><subfield code="a">Fakultät für Biowissenschaften</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">19</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">19</subfield><subfield code="s">s</subfield><subfield code="a">pos_16</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">00</subfield><subfield code="s">s</subfield><subfield code="a">hd2020</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">01</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410508463</subfield><subfield code="a">wissenschaftlicher Artikel (Zeitschrift)</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">02</subfield><subfield code="s">s</subfield><subfield code="a">per_26</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">03</subfield><subfield code="s">s</subfield><subfield code="a">s_13</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">04</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1741854938</subfield><subfield code="a">Ullrich, Stephanie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">04</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">04</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">04</subfield><subfield code="s">s</subfield><subfield code="a">pos_17</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">05</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1702121828</subfield><subfield code="a">Barreto Miranda, Isabel</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">05</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">05</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">05</subfield><subfield code="s">s</subfield><subfield code="a">pos_18</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">06</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1663598371</subfield><subfield code="a">Anders, Simon</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">06</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1416733221</subfield><subfield code="a">Zentrum für Molekulare Biologie (ZMBH)</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">06</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">06</subfield><subfield code="s">s</subfield><subfield code="a">pos_19</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">07</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1466739088</subfield><subfield code="a">Grimm, Dirk</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">07</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">07</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">07</subfield><subfield code="s">s</subfield><subfield code="a">pos_20</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">08</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1436156459</subfield><subfield code="a">Schnitzler, Paul</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">08</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">08</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1416466967</subfield><subfield code="a">Medizinische Fakultät Heidelberg</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">08</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">08</subfield><subfield code="s">s</subfield><subfield code="a">pos_21</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">09</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)149383195X</subfield><subfield code="a">Knop, Michael</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">09</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1416733221</subfield><subfield code="a">Zentrum für Molekulare Biologie (ZMBH)</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">09</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">09</subfield><subfield code="s">s</subfield><subfield code="a">pos_22</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">10</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1443450340</subfield><subfield code="a">Kräusslich, Hans-Georg</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">10</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">10</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">10</subfield><subfield code="s">s</subfield><subfield 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Steffen Klein, Thorsten G. Müller, Dina Khalid, Vera Sonntag-Buck, Anke-Mareil Heuser, Bärbel Glass, Matthias Meurer, Ivonne Morales, Angelika Schillak, Andrew Freistaedter, Ina Ambiel, Sophie L. Winter, Liv Zimmermann, Tamara Naumoska, Felix Bubeck, Daniel Kirrmaier, Stephanie Ullrich, Isabel Barreto Miranda, Simon Anders, Dirk Grimm, Paul Schnitzler, Michael Knop, Hans-Georg Kräusslich, Viet Loan Dao Thi, Kathleen Börner andnPetr Chlanda coronavirus COVID-19 diagnostics high-throughput screening magnetic bead RNA purification pandemic RNA virus RT-LAMP RT-qPCR SARS-CoV-2 |
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Klein, Steffen Müller, Thorsten G. Khalid, Dina Sonntag-Buck, Vera Heuser, Anke-Mareil Glass, Bärbel Meurer, Matthias Morales, Ivonne Schillak, Angelika Freistaedter, Andrew Ambiel, Ina Winter, Sophie L. Zimmermann, Liv Naumoska, Tamara Bubeck, Felix Kirrmaier, Daniel Ullrich, Stephanie Barreto Miranda, Isabel Anders, Simon Grimm, Dirk Schnitzler, Paul Knop, Michael Kräusslich, Hans-Georg Dao Thi, Viet Loan Börner, Kathleen Chlanda, Petr |
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sars-cov-2 rna extraction using magnetic beads for rapid large-scale testing by rt-qpcr and rt-lamp |
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SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP |
| abstract |
Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot. Gesehen am 03.12.2020 |
| abstractGer |
Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot. Gesehen am 03.12.2020 |
| abstract_unstemmed |
Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot. Gesehen am 03.12.2020 |
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SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP |
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code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">10</subfield><subfield code="s">s</subfield><subfield code="a">pos_7</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">11</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1503648370</subfield><subfield code="a">Morales, Ivonne</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">11</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">11</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">11</subfield><subfield code="s">s</subfield><subfield code="a">pos_8</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">12</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1741497779</subfield><subfield code="a">Schillack, Angelika</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">12</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">12</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">12</subfield><subfield code="s">s</subfield><subfield code="a">pos_9</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">13</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)174184309X</subfield><subfield code="a">Freistaedter, Andrew</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">13</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">13</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">13</subfield><subfield code="s">s</subfield><subfield code="a">pos_10</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield 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code="a">pos_11</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">15</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1741844908</subfield><subfield code="a">Winter, Sophie L.</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">15</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">15</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">15</subfield><subfield code="s">s</subfield><subfield code="a">pos_12</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">16</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1741845823</subfield><subfield code="a">Zimmermann, Liv</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">16</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">16</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">16</subfield><subfield code="s">s</subfield><subfield code="a">pos_13</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">17</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1741846579</subfield><subfield code="a">Naumoska, Tamara</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">17</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">17</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">17</subfield><subfield code="s">s</subfield><subfield code="a">pos_14</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">18</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1584732598</subfield><subfield code="a">Bubeck, Felix</subfield></datafield><datafield tag="982" 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code="x">DE-16-250</subfield><subfield code="8">19</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1493831461</subfield><subfield code="a">Kirrmaier, Daniel</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">19</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1416535500</subfield><subfield code="a">Fakultät für Biowissenschaften</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">19</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">01</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">19</subfield><subfield code="s">s</subfield><subfield code="a">pos_16</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">00</subfield><subfield code="s">s</subfield><subfield code="a">hd2020</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">01</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410508463</subfield><subfield code="a">wissenschaftlicher Artikel (Zeitschrift)</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">02</subfield><subfield code="s">s</subfield><subfield code="a">per_26</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">03</subfield><subfield code="s">s</subfield><subfield code="a">s_13</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">04</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1741854938</subfield><subfield code="a">Ullrich, Stephanie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">04</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">04</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">04</subfield><subfield code="s">s</subfield><subfield code="a">pos_17</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">05</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1702121828</subfield><subfield code="a">Barreto Miranda, Isabel</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">05</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">05</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">05</subfield><subfield code="s">s</subfield><subfield code="a">pos_18</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">06</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1663598371</subfield><subfield code="a">Anders, Simon</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">06</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1416733221</subfield><subfield code="a">Zentrum für Molekulare Biologie (ZMBH)</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">06</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">06</subfield><subfield code="s">s</subfield><subfield code="a">pos_19</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">07</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1466739088</subfield><subfield code="a">Grimm, Dirk</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">07</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">07</subfield><subfield code="s">s</subfield><subfield code="0">(DE-627)1410501914</subfield><subfield code="a">Verfasser</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">07</subfield><subfield code="s">s</subfield><subfield code="a">pos_20</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">08</subfield><subfield code="s">p</subfield><subfield code="0">(DE-627)1436156459</subfield><subfield code="a">Schnitzler, Paul</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">08</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1495544966</subfield><subfield code="a">Zentrum für Infektiologie</subfield></datafield><datafield tag="982" ind1=" " ind2=" "><subfield code="2">2013</subfield><subfield code="1">02</subfield><subfield code="x">DE-16-250</subfield><subfield code="8">08</subfield><subfield code="s">k</subfield><subfield code="0">(DE-627)1416466967</subfield><subfield code="a">Medizinische Fakultät 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