Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development
Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and ad...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zilova, Lucie [verfasserIn] Weinhardt, Venera [verfasserIn] Tavhelidse-Suck, Tinatini [verfasserIn] Schlagheck, Christina [verfasserIn] Thumberger, Thomas [verfasserIn] Wittbrodt, Joachim [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
12 July 2021 |
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| Schlagwörter: |
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| Anmerkung: |
Gesehen am 10.08.2021 |
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| Umfang: |
26 |
| Weitere Ausgabe: |
Forschungsdaten Zilova, Lucie: Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation - Heidelberg : Universität, 2021 |
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| Übergeordnetes Werk: |
Enthalten in: eLife - Cambridge : eLife Sciences Publications, 2012, 10(2021), Artikel-ID e66998, Seite 1-26 |
| Übergeordnetes Werk: |
volume:10 ; year:2021 ; elocationid:e66998 ; pages:1-26 ; extent:26 |
| Links: |
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| DOI / URN: |
10.7554/eLife.66998 |
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| Katalog-ID: |
1766015395 |
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| 520 | |a Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. | ||
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10.7554/eLife.66998 doi (DE-627)1766015395 (DE-599)KXP1766015395 (OCoLC)1341420512 DE-627 ger DE-627 rda eng Zilova, Lucie verfasserin (DE-588)1238632513 (DE-627)1766016189 aut Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development Lucie Zilova, Venera Weinhardt, Tinatini Tavhelidse, Christina Schlagheck, Thomas Thumberger, Joachim Wittbrodt 12 July 2021 26 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 10.08.2021 Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. anterior neuroectoderm crucial role homeobox gene neural differentiation optic-cup pigment-epithelium stem-cells transcription factor vertebrate eye zebrafish Weinhardt, Venera verfasserin (DE-588)1054684472 (DE-627)791918920 (DE-576)410432555 aut Tavhelidse-Suck, Tinatini verfasserin (DE-588)1124483764 (DE-627)878199837 (DE-576)482786531 aut Schlagheck, Christina verfasserin (DE-588)123863382X (DE-627)1766016545 aut Thumberger, Thomas verfasserin (DE-588)1026677068 (DE-627)727045091 (DE-576)354676075 aut Wittbrodt, Joachim verfasserin (DE-588)1038250919 (DE-627)756835356 (DE-576)167753401 aut Enthalten in eLife Cambridge : eLife Sciences Publications, 2012 10(2021), Artikel-ID e66998, Seite 1-26 Online-Ressource (DE-627)728518384 (DE-600)2687154-3 (DE-576)372567576 2050-084X nnns volume:10 year:2021 elocationid:e66998 pages:1-26 extent:26 Forschungsdaten Zilova, Lucie Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation Heidelberg : Universität, 2021 1 Online-Ressource (12 Files) (DE-627)179531687X https://doi.org/10.7554/eLife.66998 Verlag Resolving-System kostenfrei Volltext https://elifesciences.org/articles/66998 Verlag kostenfrei Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_2403 GBV_ILN_2403 ISIL_DE-LFER AR 10 2021 e66998 1-26 26 2013 01 DE-16-250 3964483990 00 --%%-- --%%-- --%%-- --%%-- l01 10-08-21 2403 01 DE-LFER 411174267X 00 --%%-- --%%-- n --%%-- l01 04-04-22 2403 01 DE-LFER https://doi.org/10.7554/eLife.66998 2403 01 DE-LFER https://elifesciences.org/articles/66998 2013 01 DE-16-250 00 s hd2021 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_6 2013 01 DE-16-250 03 s s_26 2013 01 DE-16-250 04 p (DE-627)1766017207 Zilova, Lucie 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1580066895 Weinhardt, Venera 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1552786889 Tavhelidse-Suck, Tinatini 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1795320036 Schlagheck, Christina 2013 01 DE-16-250 07 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1512966347 Thumberger, Thomas 2013 01 DE-16-250 08 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1462234100 Wittbrodt, Joachim 2013 01 DE-16-250 09 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 |
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10.7554/eLife.66998 doi (DE-627)1766015395 (DE-599)KXP1766015395 (OCoLC)1341420512 DE-627 ger DE-627 rda eng Zilova, Lucie verfasserin (DE-588)1238632513 (DE-627)1766016189 aut Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development Lucie Zilova, Venera Weinhardt, Tinatini Tavhelidse, Christina Schlagheck, Thomas Thumberger, Joachim Wittbrodt 12 July 2021 26 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 10.08.2021 Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. anterior neuroectoderm crucial role homeobox gene neural differentiation optic-cup pigment-epithelium stem-cells transcription factor vertebrate eye zebrafish Weinhardt, Venera verfasserin (DE-588)1054684472 (DE-627)791918920 (DE-576)410432555 aut Tavhelidse-Suck, Tinatini verfasserin (DE-588)1124483764 (DE-627)878199837 (DE-576)482786531 aut Schlagheck, Christina verfasserin (DE-588)123863382X (DE-627)1766016545 aut Thumberger, Thomas verfasserin (DE-588)1026677068 (DE-627)727045091 (DE-576)354676075 aut Wittbrodt, Joachim verfasserin (DE-588)1038250919 (DE-627)756835356 (DE-576)167753401 aut Enthalten in eLife Cambridge : eLife Sciences Publications, 2012 10(2021), Artikel-ID e66998, Seite 1-26 Online-Ressource (DE-627)728518384 (DE-600)2687154-3 (DE-576)372567576 2050-084X nnns volume:10 year:2021 elocationid:e66998 pages:1-26 extent:26 Forschungsdaten Zilova, Lucie Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation Heidelberg : Universität, 2021 1 Online-Ressource (12 Files) (DE-627)179531687X https://doi.org/10.7554/eLife.66998 Verlag Resolving-System kostenfrei Volltext https://elifesciences.org/articles/66998 Verlag kostenfrei Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_2403 GBV_ILN_2403 ISIL_DE-LFER AR 10 2021 e66998 1-26 26 2013 01 DE-16-250 3964483990 00 --%%-- --%%-- --%%-- --%%-- l01 10-08-21 2403 01 DE-LFER 411174267X 00 --%%-- --%%-- n --%%-- l01 04-04-22 2403 01 DE-LFER https://doi.org/10.7554/eLife.66998 2403 01 DE-LFER https://elifesciences.org/articles/66998 2013 01 DE-16-250 00 s hd2021 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_6 2013 01 DE-16-250 03 s s_26 2013 01 DE-16-250 04 p (DE-627)1766017207 Zilova, Lucie 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1580066895 Weinhardt, Venera 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1552786889 Tavhelidse-Suck, Tinatini 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1795320036 Schlagheck, Christina 2013 01 DE-16-250 07 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1512966347 Thumberger, Thomas 2013 01 DE-16-250 08 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1462234100 Wittbrodt, Joachim 2013 01 DE-16-250 09 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 |
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10.7554/eLife.66998 doi (DE-627)1766015395 (DE-599)KXP1766015395 (OCoLC)1341420512 DE-627 ger DE-627 rda eng Zilova, Lucie verfasserin (DE-588)1238632513 (DE-627)1766016189 aut Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development Lucie Zilova, Venera Weinhardt, Tinatini Tavhelidse, Christina Schlagheck, Thomas Thumberger, Joachim Wittbrodt 12 July 2021 26 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 10.08.2021 Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. anterior neuroectoderm crucial role homeobox gene neural differentiation optic-cup pigment-epithelium stem-cells transcription factor vertebrate eye zebrafish Weinhardt, Venera verfasserin (DE-588)1054684472 (DE-627)791918920 (DE-576)410432555 aut Tavhelidse-Suck, Tinatini verfasserin (DE-588)1124483764 (DE-627)878199837 (DE-576)482786531 aut Schlagheck, Christina verfasserin (DE-588)123863382X (DE-627)1766016545 aut Thumberger, Thomas verfasserin (DE-588)1026677068 (DE-627)727045091 (DE-576)354676075 aut Wittbrodt, Joachim verfasserin (DE-588)1038250919 (DE-627)756835356 (DE-576)167753401 aut Enthalten in eLife Cambridge : eLife Sciences Publications, 2012 10(2021), Artikel-ID e66998, Seite 1-26 Online-Ressource (DE-627)728518384 (DE-600)2687154-3 (DE-576)372567576 2050-084X nnns volume:10 year:2021 elocationid:e66998 pages:1-26 extent:26 Forschungsdaten Zilova, Lucie Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation Heidelberg : Universität, 2021 1 Online-Ressource (12 Files) (DE-627)179531687X https://doi.org/10.7554/eLife.66998 Verlag Resolving-System kostenfrei Volltext https://elifesciences.org/articles/66998 Verlag kostenfrei Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_2403 GBV_ILN_2403 ISIL_DE-LFER AR 10 2021 e66998 1-26 26 2013 01 DE-16-250 3964483990 00 --%%-- --%%-- --%%-- --%%-- l01 10-08-21 2403 01 DE-LFER 411174267X 00 --%%-- --%%-- n --%%-- l01 04-04-22 2403 01 DE-LFER https://doi.org/10.7554/eLife.66998 2403 01 DE-LFER https://elifesciences.org/articles/66998 2013 01 DE-16-250 00 s hd2021 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_6 2013 01 DE-16-250 03 s s_26 2013 01 DE-16-250 04 p (DE-627)1766017207 Zilova, Lucie 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1580066895 Weinhardt, Venera 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1552786889 Tavhelidse-Suck, Tinatini 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1795320036 Schlagheck, Christina 2013 01 DE-16-250 07 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1512966347 Thumberger, Thomas 2013 01 DE-16-250 08 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1462234100 Wittbrodt, Joachim 2013 01 DE-16-250 09 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 |
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10.7554/eLife.66998 doi (DE-627)1766015395 (DE-599)KXP1766015395 (OCoLC)1341420512 DE-627 ger DE-627 rda eng Zilova, Lucie verfasserin (DE-588)1238632513 (DE-627)1766016189 aut Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development Lucie Zilova, Venera Weinhardt, Tinatini Tavhelidse, Christina Schlagheck, Thomas Thumberger, Joachim Wittbrodt 12 July 2021 26 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 10.08.2021 Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. anterior neuroectoderm crucial role homeobox gene neural differentiation optic-cup pigment-epithelium stem-cells transcription factor vertebrate eye zebrafish Weinhardt, Venera verfasserin (DE-588)1054684472 (DE-627)791918920 (DE-576)410432555 aut Tavhelidse-Suck, Tinatini verfasserin (DE-588)1124483764 (DE-627)878199837 (DE-576)482786531 aut Schlagheck, Christina verfasserin (DE-588)123863382X (DE-627)1766016545 aut Thumberger, Thomas verfasserin (DE-588)1026677068 (DE-627)727045091 (DE-576)354676075 aut Wittbrodt, Joachim verfasserin (DE-588)1038250919 (DE-627)756835356 (DE-576)167753401 aut Enthalten in eLife Cambridge : eLife Sciences Publications, 2012 10(2021), Artikel-ID e66998, Seite 1-26 Online-Ressource (DE-627)728518384 (DE-600)2687154-3 (DE-576)372567576 2050-084X nnns volume:10 year:2021 elocationid:e66998 pages:1-26 extent:26 Forschungsdaten Zilova, Lucie Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation Heidelberg : Universität, 2021 1 Online-Ressource (12 Files) (DE-627)179531687X https://doi.org/10.7554/eLife.66998 Verlag Resolving-System kostenfrei Volltext https://elifesciences.org/articles/66998 Verlag kostenfrei Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_2403 GBV_ILN_2403 ISIL_DE-LFER AR 10 2021 e66998 1-26 26 2013 01 DE-16-250 3964483990 00 --%%-- --%%-- --%%-- --%%-- l01 10-08-21 2403 01 DE-LFER 411174267X 00 --%%-- --%%-- n --%%-- l01 04-04-22 2403 01 DE-LFER https://doi.org/10.7554/eLife.66998 2403 01 DE-LFER https://elifesciences.org/articles/66998 2013 01 DE-16-250 00 s hd2021 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_6 2013 01 DE-16-250 03 s s_26 2013 01 DE-16-250 04 p (DE-627)1766017207 Zilova, Lucie 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1580066895 Weinhardt, Venera 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1552786889 Tavhelidse-Suck, Tinatini 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1795320036 Schlagheck, Christina 2013 01 DE-16-250 07 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1512966347 Thumberger, Thomas 2013 01 DE-16-250 08 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1462234100 Wittbrodt, Joachim 2013 01 DE-16-250 09 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 |
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10.7554/eLife.66998 doi (DE-627)1766015395 (DE-599)KXP1766015395 (OCoLC)1341420512 DE-627 ger DE-627 rda eng Zilova, Lucie verfasserin (DE-588)1238632513 (DE-627)1766016189 aut Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development Lucie Zilova, Venera Weinhardt, Tinatini Tavhelidse, Christina Schlagheck, Thomas Thumberger, Joachim Wittbrodt 12 July 2021 26 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 10.08.2021 Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. anterior neuroectoderm crucial role homeobox gene neural differentiation optic-cup pigment-epithelium stem-cells transcription factor vertebrate eye zebrafish Weinhardt, Venera verfasserin (DE-588)1054684472 (DE-627)791918920 (DE-576)410432555 aut Tavhelidse-Suck, Tinatini verfasserin (DE-588)1124483764 (DE-627)878199837 (DE-576)482786531 aut Schlagheck, Christina verfasserin (DE-588)123863382X (DE-627)1766016545 aut Thumberger, Thomas verfasserin (DE-588)1026677068 (DE-627)727045091 (DE-576)354676075 aut Wittbrodt, Joachim verfasserin (DE-588)1038250919 (DE-627)756835356 (DE-576)167753401 aut Enthalten in eLife Cambridge : eLife Sciences Publications, 2012 10(2021), Artikel-ID e66998, Seite 1-26 Online-Ressource (DE-627)728518384 (DE-600)2687154-3 (DE-576)372567576 2050-084X nnns volume:10 year:2021 elocationid:e66998 pages:1-26 extent:26 Forschungsdaten Zilova, Lucie Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation Heidelberg : Universität, 2021 1 Online-Ressource (12 Files) (DE-627)179531687X https://doi.org/10.7554/eLife.66998 Verlag Resolving-System kostenfrei Volltext https://elifesciences.org/articles/66998 Verlag kostenfrei Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_2403 GBV_ILN_2403 ISIL_DE-LFER AR 10 2021 e66998 1-26 26 2013 01 DE-16-250 3964483990 00 --%%-- --%%-- --%%-- --%%-- l01 10-08-21 2403 01 DE-LFER 411174267X 00 --%%-- --%%-- n --%%-- l01 04-04-22 2403 01 DE-LFER https://doi.org/10.7554/eLife.66998 2403 01 DE-LFER https://elifesciences.org/articles/66998 2013 01 DE-16-250 00 s hd2021 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_6 2013 01 DE-16-250 03 s s_26 2013 01 DE-16-250 04 p (DE-627)1766017207 Zilova, Lucie 2013 01 DE-16-250 04 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1580066895 Weinhardt, Venera 2013 01 DE-16-250 05 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_2 2013 01 DE-16-250 06 p (DE-627)1552786889 Tavhelidse-Suck, Tinatini 2013 01 DE-16-250 06 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_3 2013 01 DE-16-250 07 p (DE-627)1795320036 Schlagheck, Christina 2013 01 DE-16-250 07 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_4 2013 01 DE-16-250 08 p (DE-627)1512966347 Thumberger, Thomas 2013 01 DE-16-250 08 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_5 2013 01 DE-16-250 09 p (DE-627)1462234100 Wittbrodt, Joachim 2013 01 DE-16-250 09 k (DE-627)1416737987 Centre for Organismal Studies Heidelberg (COS) 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_6 |
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English |
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Enthalten in eLife 10(2021), Artikel-ID e66998, Seite 1-26 volume:10 year:2021 elocationid:e66998 pages:1-26 extent:26 |
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Enthalten in eLife 10(2021), Artikel-ID e66998, Seite 1-26 volume:10 year:2021 elocationid:e66998 pages:1-26 extent:26 |
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Article |
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2013:0 2403:0 |
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findex.gbv.de |
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anterior neuroectoderm crucial role homeobox gene neural differentiation optic-cup pigment-epithelium stem-cells transcription factor vertebrate eye zebrafish |
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eLife |
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Zilova, Lucie @@aut@@ Weinhardt, Venera @@aut@@ Tavhelidse-Suck, Tinatini @@aut@@ Schlagheck, Christina @@aut@@ Thumberger, Thomas @@aut@@ Wittbrodt, Joachim @@aut@@ |
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2021-01-01T00:00:00Z |
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728518384 |
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1766015395 |
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englisch |
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fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development |
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Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development |
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Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. Gesehen am 10.08.2021 Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation |
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Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. Gesehen am 10.08.2021 Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation |
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Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. Gesehen am 10.08.2021 Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation |
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