Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia

Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequent...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Müller, Martin Christian - 1972- [verfasserIn] Hördt, Tanja [verfasserIn] Paschka, Peter - 1970- [verfasserIn] Merx, Kirsten - 1970- [verfasserIn] La Rosée, Paul - 1969- [verfasserIn] Hehlmann, Rüdiger - 1941- [verfasserIn] Hochhaus, Andreas - 1959- [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	May 2004

Anmerkung:	Gesehen am 13.05.2022
Umfang:	4

Übergeordnetes Werk:	Enthalten in: Acta haematologica - Basel : Karger, 1948, 112(2004), 1-2, Seite 30-33
Übergeordnetes Werk:	volume:112 ; year:2004 ; number:1-2 ; pages:30-33 ; extent:4

Links:	Volltext Volltext

DOI / URN:	10.1159/000077557

Katalog-ID:	1801724512

Internformat


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245	1	0	\|a Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia \|c Martin C. Müller, Tanja Hördt, Peter Paschka, Kirsten Merx, Paul La Rosée, Rüdiger Hehlmann, Andreas Hochhaus
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520			\|a Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequently the validity of MRD results are reviewed. RNA degradation in unstabilized peripheral blood (PB) samples does not play a major role in samples being processed on the day of blood collection. However, the simulation of sample shipping at room temperature with a delay of sample processing of up to 3 days causes a dramatic loss of intact RNA. RNA degradation can be prevented by the use of a bedside RNA stabilization system. Additionally, the stabilizing procedure is capable of keeping real-time quantitative polymerase chain reaction (RQ-PCR) results comparable whether the sample is processed immediately or with a delay of up to 3 days. Consistent quantitative data cannot be obtained with unstabilized blood samples. Furthermore, the optimum volume of PB required for MRD diagnostics in patients with BCR-ABL-positive chronic myelogenous leukemia in complete cytogenetic remission is revisited. Ten milliliters of PB is sufficient for processing on the day of blood collection whereas the use of only 5 ml PB may result in false-negative results. Standardization of preanalytical and analytical factors is necessary to provide a comparability of RQ-PCR results from different laboratories within multicenter studies. The definition of ‘undetectable BCR-ABL’ in an individual patient should take these preanalytical parameters into consideration.
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allfields	10.1159/000077557 doi (DE-627)1801724512 (DE-599)KXP1801724512 (OCoLC)1341460048 DE-627 ger DE-627 rda eng Müller, Martin Christian 1972- verfasserin (DE-588)121360296 (DE-627)705409236 (DE-576)181482819 aut Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia Martin C. Müller, Tanja Hördt, Peter Paschka, Kirsten Merx, Paul La Rosée, Rüdiger Hehlmann, Andreas Hochhaus May 2004 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 13.05.2022 Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequently the validity of MRD results are reviewed. RNA degradation in unstabilized peripheral blood (PB) samples does not play a major role in samples being processed on the day of blood collection. However, the simulation of sample shipping at room temperature with a delay of sample processing of up to 3 days causes a dramatic loss of intact RNA. RNA degradation can be prevented by the use of a bedside RNA stabilization system. Additionally, the stabilizing procedure is capable of keeping real-time quantitative polymerase chain reaction (RQ-PCR) results comparable whether the sample is processed immediately or with a delay of up to 3 days. Consistent quantitative data cannot be obtained with unstabilized blood samples. Furthermore, the optimum volume of PB required for MRD diagnostics in patients with BCR-ABL-positive chronic myelogenous leukemia in complete cytogenetic remission is revisited. Ten milliliters of PB is sufficient for processing on the day of blood collection whereas the use of only 5 ml PB may result in false-negative results. Standardization of preanalytical and analytical factors is necessary to provide a comparability of RQ-PCR results from different laboratories within multicenter studies. The definition of ‘undetectable BCR-ABL’ in an individual patient should take these preanalytical parameters into consideration. Hördt, Tanja verfasserin aut Paschka, Peter 1970- verfasserin (DE-588)123089840 (DE-627)706156870 (DE-576)293550832 aut Merx, Kirsten 1970- verfasserin (DE-588)122413091 (DE-627)70589892X (DE-576)293258910 aut La Rosée, Paul 1969- verfasserin (DE-588)122116674 (DE-627)081745966 (DE-576)293100357 aut Hehlmann, Rüdiger 1941- verfasserin (DE-588)1037003489 (DE-627)751737879 (DE-576)390939463 aut Hochhaus, Andreas 1959- verfasserin (DE-588)1107039339 (DE-627)863321976 (DE-576)474976130 aut Enthalten in Acta haematologica Basel : Karger, 1948 112(2004), 1-2, Seite 30-33 (DE-627)300188080 (DE-600)1481888-7 (DE-576)252619641 1421-9662 nnns volume:112 year:2004 number:1-2 pages:30-33 extent:4 https://doi.org/10.1159/000077557 Verlag Resolving-System lizenzpflichtig Volltext https://www.karger.com/Article/FullText/77557 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_120 GBV_ILN_121 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2886 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4753 AR 112 2004 1-2 30-33 4 2013 01 DE-16-250 4133922549 00 --%%-- --%%-- --%%-- --%%-- l01 13-05-22 2013 01 DE-16-250 00 s hd2004 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_7 2013 01 DE-16-250 03 s s_4 2013 01 DE-16-250 04 p (DE-627)1445829509 Müller, Martin Christian 2013 01 DE-16-250 04 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1799509028 Paschka, Peter 2013 01 DE-16-250 05 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1460938518 Merx, Kirsten 2013 01 DE-16-250 06 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 2013 01 DE-16-250 07 p (DE-627)1514972700 La Rosée, Paul 2013 01 DE-16-250 07 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_5 2013 01 DE-16-250 08 p (DE-627)1460943260 Hehlmann, Rüdiger 2013 01 DE-16-250 08 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_6 2013 01 DE-16-250 09 p (DE-627)1544976704 Hochhaus, Andreas 2013 01 DE-16-250 09 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_7
spelling	10.1159/000077557 doi (DE-627)1801724512 (DE-599)KXP1801724512 (OCoLC)1341460048 DE-627 ger DE-627 rda eng Müller, Martin Christian 1972- verfasserin (DE-588)121360296 (DE-627)705409236 (DE-576)181482819 aut Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia Martin C. Müller, Tanja Hördt, Peter Paschka, Kirsten Merx, Paul La Rosée, Rüdiger Hehlmann, Andreas Hochhaus May 2004 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 13.05.2022 Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequently the validity of MRD results are reviewed. RNA degradation in unstabilized peripheral blood (PB) samples does not play a major role in samples being processed on the day of blood collection. However, the simulation of sample shipping at room temperature with a delay of sample processing of up to 3 days causes a dramatic loss of intact RNA. RNA degradation can be prevented by the use of a bedside RNA stabilization system. Additionally, the stabilizing procedure is capable of keeping real-time quantitative polymerase chain reaction (RQ-PCR) results comparable whether the sample is processed immediately or with a delay of up to 3 days. Consistent quantitative data cannot be obtained with unstabilized blood samples. Furthermore, the optimum volume of PB required for MRD diagnostics in patients with BCR-ABL-positive chronic myelogenous leukemia in complete cytogenetic remission is revisited. Ten milliliters of PB is sufficient for processing on the day of blood collection whereas the use of only 5 ml PB may result in false-negative results. Standardization of preanalytical and analytical factors is necessary to provide a comparability of RQ-PCR results from different laboratories within multicenter studies. The definition of ‘undetectable BCR-ABL’ in an individual patient should take these preanalytical parameters into consideration. Hördt, Tanja verfasserin aut Paschka, Peter 1970- verfasserin (DE-588)123089840 (DE-627)706156870 (DE-576)293550832 aut Merx, Kirsten 1970- verfasserin (DE-588)122413091 (DE-627)70589892X (DE-576)293258910 aut La Rosée, Paul 1969- verfasserin (DE-588)122116674 (DE-627)081745966 (DE-576)293100357 aut Hehlmann, Rüdiger 1941- verfasserin (DE-588)1037003489 (DE-627)751737879 (DE-576)390939463 aut Hochhaus, Andreas 1959- verfasserin (DE-588)1107039339 (DE-627)863321976 (DE-576)474976130 aut Enthalten in Acta haematologica Basel : Karger, 1948 112(2004), 1-2, Seite 30-33 (DE-627)300188080 (DE-600)1481888-7 (DE-576)252619641 1421-9662 nnns volume:112 year:2004 number:1-2 pages:30-33 extent:4 https://doi.org/10.1159/000077557 Verlag Resolving-System lizenzpflichtig Volltext https://www.karger.com/Article/FullText/77557 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_120 GBV_ILN_121 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2886 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4753 AR 112 2004 1-2 30-33 4 2013 01 DE-16-250 4133922549 00 --%%-- --%%-- --%%-- --%%-- l01 13-05-22 2013 01 DE-16-250 00 s hd2004 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_7 2013 01 DE-16-250 03 s s_4 2013 01 DE-16-250 04 p (DE-627)1445829509 Müller, Martin Christian 2013 01 DE-16-250 04 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1799509028 Paschka, Peter 2013 01 DE-16-250 05 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1460938518 Merx, Kirsten 2013 01 DE-16-250 06 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 2013 01 DE-16-250 07 p (DE-627)1514972700 La Rosée, Paul 2013 01 DE-16-250 07 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_5 2013 01 DE-16-250 08 p (DE-627)1460943260 Hehlmann, Rüdiger 2013 01 DE-16-250 08 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_6 2013 01 DE-16-250 09 p (DE-627)1544976704 Hochhaus, Andreas 2013 01 DE-16-250 09 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_7
allfields_unstemmed	10.1159/000077557 doi (DE-627)1801724512 (DE-599)KXP1801724512 (OCoLC)1341460048 DE-627 ger DE-627 rda eng Müller, Martin Christian 1972- verfasserin (DE-588)121360296 (DE-627)705409236 (DE-576)181482819 aut Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia Martin C. Müller, Tanja Hördt, Peter Paschka, Kirsten Merx, Paul La Rosée, Rüdiger Hehlmann, Andreas Hochhaus May 2004 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 13.05.2022 Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequently the validity of MRD results are reviewed. RNA degradation in unstabilized peripheral blood (PB) samples does not play a major role in samples being processed on the day of blood collection. However, the simulation of sample shipping at room temperature with a delay of sample processing of up to 3 days causes a dramatic loss of intact RNA. RNA degradation can be prevented by the use of a bedside RNA stabilization system. Additionally, the stabilizing procedure is capable of keeping real-time quantitative polymerase chain reaction (RQ-PCR) results comparable whether the sample is processed immediately or with a delay of up to 3 days. Consistent quantitative data cannot be obtained with unstabilized blood samples. Furthermore, the optimum volume of PB required for MRD diagnostics in patients with BCR-ABL-positive chronic myelogenous leukemia in complete cytogenetic remission is revisited. Ten milliliters of PB is sufficient for processing on the day of blood collection whereas the use of only 5 ml PB may result in false-negative results. Standardization of preanalytical and analytical factors is necessary to provide a comparability of RQ-PCR results from different laboratories within multicenter studies. The definition of ‘undetectable BCR-ABL’ in an individual patient should take these preanalytical parameters into consideration. Hördt, Tanja verfasserin aut Paschka, Peter 1970- verfasserin (DE-588)123089840 (DE-627)706156870 (DE-576)293550832 aut Merx, Kirsten 1970- verfasserin (DE-588)122413091 (DE-627)70589892X (DE-576)293258910 aut La Rosée, Paul 1969- verfasserin (DE-588)122116674 (DE-627)081745966 (DE-576)293100357 aut Hehlmann, Rüdiger 1941- verfasserin (DE-588)1037003489 (DE-627)751737879 (DE-576)390939463 aut Hochhaus, Andreas 1959- verfasserin (DE-588)1107039339 (DE-627)863321976 (DE-576)474976130 aut Enthalten in Acta haematologica Basel : Karger, 1948 112(2004), 1-2, Seite 30-33 (DE-627)300188080 (DE-600)1481888-7 (DE-576)252619641 1421-9662 nnns volume:112 year:2004 number:1-2 pages:30-33 extent:4 https://doi.org/10.1159/000077557 Verlag Resolving-System lizenzpflichtig Volltext https://www.karger.com/Article/FullText/77557 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_120 GBV_ILN_121 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2886 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4753 AR 112 2004 1-2 30-33 4 2013 01 DE-16-250 4133922549 00 --%%-- --%%-- --%%-- --%%-- l01 13-05-22 2013 01 DE-16-250 00 s hd2004 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_7 2013 01 DE-16-250 03 s s_4 2013 01 DE-16-250 04 p (DE-627)1445829509 Müller, Martin Christian 2013 01 DE-16-250 04 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1799509028 Paschka, Peter 2013 01 DE-16-250 05 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1460938518 Merx, Kirsten 2013 01 DE-16-250 06 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 2013 01 DE-16-250 07 p (DE-627)1514972700 La Rosée, Paul 2013 01 DE-16-250 07 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_5 2013 01 DE-16-250 08 p (DE-627)1460943260 Hehlmann, Rüdiger 2013 01 DE-16-250 08 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_6 2013 01 DE-16-250 09 p (DE-627)1544976704 Hochhaus, Andreas 2013 01 DE-16-250 09 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_7
allfieldsGer	10.1159/000077557 doi (DE-627)1801724512 (DE-599)KXP1801724512 (OCoLC)1341460048 DE-627 ger DE-627 rda eng Müller, Martin Christian 1972- verfasserin (DE-588)121360296 (DE-627)705409236 (DE-576)181482819 aut Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia Martin C. Müller, Tanja Hördt, Peter Paschka, Kirsten Merx, Paul La Rosée, Rüdiger Hehlmann, Andreas Hochhaus May 2004 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 13.05.2022 Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequently the validity of MRD results are reviewed. RNA degradation in unstabilized peripheral blood (PB) samples does not play a major role in samples being processed on the day of blood collection. However, the simulation of sample shipping at room temperature with a delay of sample processing of up to 3 days causes a dramatic loss of intact RNA. RNA degradation can be prevented by the use of a bedside RNA stabilization system. Additionally, the stabilizing procedure is capable of keeping real-time quantitative polymerase chain reaction (RQ-PCR) results comparable whether the sample is processed immediately or with a delay of up to 3 days. Consistent quantitative data cannot be obtained with unstabilized blood samples. Furthermore, the optimum volume of PB required for MRD diagnostics in patients with BCR-ABL-positive chronic myelogenous leukemia in complete cytogenetic remission is revisited. Ten milliliters of PB is sufficient for processing on the day of blood collection whereas the use of only 5 ml PB may result in false-negative results. Standardization of preanalytical and analytical factors is necessary to provide a comparability of RQ-PCR results from different laboratories within multicenter studies. The definition of ‘undetectable BCR-ABL’ in an individual patient should take these preanalytical parameters into consideration. Hördt, Tanja verfasserin aut Paschka, Peter 1970- verfasserin (DE-588)123089840 (DE-627)706156870 (DE-576)293550832 aut Merx, Kirsten 1970- verfasserin (DE-588)122413091 (DE-627)70589892X (DE-576)293258910 aut La Rosée, Paul 1969- verfasserin (DE-588)122116674 (DE-627)081745966 (DE-576)293100357 aut Hehlmann, Rüdiger 1941- verfasserin (DE-588)1037003489 (DE-627)751737879 (DE-576)390939463 aut Hochhaus, Andreas 1959- verfasserin (DE-588)1107039339 (DE-627)863321976 (DE-576)474976130 aut Enthalten in Acta haematologica Basel : Karger, 1948 112(2004), 1-2, Seite 30-33 (DE-627)300188080 (DE-600)1481888-7 (DE-576)252619641 1421-9662 nnns volume:112 year:2004 number:1-2 pages:30-33 extent:4 https://doi.org/10.1159/000077557 Verlag Resolving-System lizenzpflichtig Volltext https://www.karger.com/Article/FullText/77557 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_120 GBV_ILN_121 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2886 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4753 AR 112 2004 1-2 30-33 4 2013 01 DE-16-250 4133922549 00 --%%-- --%%-- --%%-- --%%-- l01 13-05-22 2013 01 DE-16-250 00 s hd2004 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_7 2013 01 DE-16-250 03 s s_4 2013 01 DE-16-250 04 p (DE-627)1445829509 Müller, Martin Christian 2013 01 DE-16-250 04 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1799509028 Paschka, Peter 2013 01 DE-16-250 05 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1460938518 Merx, Kirsten 2013 01 DE-16-250 06 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 2013 01 DE-16-250 07 p (DE-627)1514972700 La Rosée, Paul 2013 01 DE-16-250 07 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_5 2013 01 DE-16-250 08 p (DE-627)1460943260 Hehlmann, Rüdiger 2013 01 DE-16-250 08 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_6 2013 01 DE-16-250 09 p (DE-627)1544976704 Hochhaus, Andreas 2013 01 DE-16-250 09 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_7
allfieldsSound	10.1159/000077557 doi (DE-627)1801724512 (DE-599)KXP1801724512 (OCoLC)1341460048 DE-627 ger DE-627 rda eng Müller, Martin Christian 1972- verfasserin (DE-588)121360296 (DE-627)705409236 (DE-576)181482819 aut Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia Martin C. Müller, Tanja Hördt, Peter Paschka, Kirsten Merx, Paul La Rosée, Rüdiger Hehlmann, Andreas Hochhaus May 2004 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gesehen am 13.05.2022 Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequently the validity of MRD results are reviewed. RNA degradation in unstabilized peripheral blood (PB) samples does not play a major role in samples being processed on the day of blood collection. However, the simulation of sample shipping at room temperature with a delay of sample processing of up to 3 days causes a dramatic loss of intact RNA. RNA degradation can be prevented by the use of a bedside RNA stabilization system. Additionally, the stabilizing procedure is capable of keeping real-time quantitative polymerase chain reaction (RQ-PCR) results comparable whether the sample is processed immediately or with a delay of up to 3 days. Consistent quantitative data cannot be obtained with unstabilized blood samples. Furthermore, the optimum volume of PB required for MRD diagnostics in patients with BCR-ABL-positive chronic myelogenous leukemia in complete cytogenetic remission is revisited. Ten milliliters of PB is sufficient for processing on the day of blood collection whereas the use of only 5 ml PB may result in false-negative results. Standardization of preanalytical and analytical factors is necessary to provide a comparability of RQ-PCR results from different laboratories within multicenter studies. The definition of ‘undetectable BCR-ABL’ in an individual patient should take these preanalytical parameters into consideration. Hördt, Tanja verfasserin aut Paschka, Peter 1970- verfasserin (DE-588)123089840 (DE-627)706156870 (DE-576)293550832 aut Merx, Kirsten 1970- verfasserin (DE-588)122413091 (DE-627)70589892X (DE-576)293258910 aut La Rosée, Paul 1969- verfasserin (DE-588)122116674 (DE-627)081745966 (DE-576)293100357 aut Hehlmann, Rüdiger 1941- verfasserin (DE-588)1037003489 (DE-627)751737879 (DE-576)390939463 aut Hochhaus, Andreas 1959- verfasserin (DE-588)1107039339 (DE-627)863321976 (DE-576)474976130 aut Enthalten in Acta haematologica Basel : Karger, 1948 112(2004), 1-2, Seite 30-33 (DE-627)300188080 (DE-600)1481888-7 (DE-576)252619641 1421-9662 nnns volume:112 year:2004 number:1-2 pages:30-33 extent:4 https://doi.org/10.1159/000077557 Verlag Resolving-System lizenzpflichtig Volltext https://www.karger.com/Article/FullText/77557 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_120 GBV_ILN_121 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2886 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4753 AR 112 2004 1-2 30-33 4 2013 01 DE-16-250 4133922549 00 --%%-- --%%-- --%%-- --%%-- l01 13-05-22 2013 01 DE-16-250 00 s hd2004 2013 01 DE-16-250 01 s (DE-627)1410508463 wissenschaftlicher Artikel (Zeitschrift) 2013 01 DE-16-250 02 s per_7 2013 01 DE-16-250 03 s s_4 2013 01 DE-16-250 04 p (DE-627)1445829509 Müller, Martin Christian 2013 01 DE-16-250 04 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_1 2013 01 DE-16-250 05 p (DE-627)1799509028 Paschka, Peter 2013 01 DE-16-250 05 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1460938518 Merx, Kirsten 2013 01 DE-16-250 06 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 2013 01 DE-16-250 07 p (DE-627)1514972700 La Rosée, Paul 2013 01 DE-16-250 07 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 07 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 07 s pos_5 2013 01 DE-16-250 08 p (DE-627)1460943260 Hehlmann, Rüdiger 2013 01 DE-16-250 08 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 08 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 08 s pos_6 2013 01 DE-16-250 09 p (DE-627)1544976704 Hochhaus, Andreas 2013 01 DE-16-250 09 k (DE-627)1416468528 III. Medizinische Klinik 2013 01 DE-16-250 09 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 09 s pos_7
language	English
source	Enthalten in Acta haematologica 112(2004), 1-2, Seite 30-33 volume:112 year:2004 number:1-2 pages:30-33 extent:4
sourceStr	Enthalten in Acta haematologica 112(2004), 1-2, Seite 30-33 volume:112 year:2004 number:1-2 pages:30-33 extent:4
format_phy_str_mv	Article
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institution	findex.gbv.de
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container_title	Acta haematologica
authorswithroles_txt_mv	Müller, Martin Christian @@aut@@ Hördt, Tanja @@aut@@ Paschka, Peter @@aut@@ Merx, Kirsten @@aut@@ La Rosée, Paul @@aut@@ Hehlmann, Rüdiger @@aut@@ Hochhaus, Andreas @@aut@@
publishDateDaySort_date	2004-01-01T00:00:00Z
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title	Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia
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title_full	Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia Martin C. Müller, Tanja Hördt, Peter Paschka, Kirsten Merx, Paul La Rosée, Rüdiger Hehlmann, Andreas Hochhaus
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title_sort	standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia
title_auth	Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia
abstract	Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequently the validity of MRD results are reviewed. RNA degradation in unstabilized peripheral blood (PB) samples does not play a major role in samples being processed on the day of blood collection. However, the simulation of sample shipping at room temperature with a delay of sample processing of up to 3 days causes a dramatic loss of intact RNA. RNA degradation can be prevented by the use of a bedside RNA stabilization system. Additionally, the stabilizing procedure is capable of keeping real-time quantitative polymerase chain reaction (RQ-PCR) results comparable whether the sample is processed immediately or with a delay of up to 3 days. Consistent quantitative data cannot be obtained with unstabilized blood samples. Furthermore, the optimum volume of PB required for MRD diagnostics in patients with BCR-ABL-positive chronic myelogenous leukemia in complete cytogenetic remission is revisited. Ten milliliters of PB is sufficient for processing on the day of blood collection whereas the use of only 5 ml PB may result in false-negative results. Standardization of preanalytical and analytical factors is necessary to provide a comparability of RQ-PCR results from different laboratories within multicenter studies. The definition of ‘undetectable BCR-ABL’ in an individual patient should take these preanalytical parameters into consideration. Gesehen am 13.05.2022
abstractGer	Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequently the validity of MRD results are reviewed. RNA degradation in unstabilized peripheral blood (PB) samples does not play a major role in samples being processed on the day of blood collection. However, the simulation of sample shipping at room temperature with a delay of sample processing of up to 3 days causes a dramatic loss of intact RNA. RNA degradation can be prevented by the use of a bedside RNA stabilization system. Additionally, the stabilizing procedure is capable of keeping real-time quantitative polymerase chain reaction (RQ-PCR) results comparable whether the sample is processed immediately or with a delay of up to 3 days. Consistent quantitative data cannot be obtained with unstabilized blood samples. Furthermore, the optimum volume of PB required for MRD diagnostics in patients with BCR-ABL-positive chronic myelogenous leukemia in complete cytogenetic remission is revisited. Ten milliliters of PB is sufficient for processing on the day of blood collection whereas the use of only 5 ml PB may result in false-negative results. Standardization of preanalytical and analytical factors is necessary to provide a comparability of RQ-PCR results from different laboratories within multicenter studies. The definition of ‘undetectable BCR-ABL’ in an individual patient should take these preanalytical parameters into consideration. Gesehen am 13.05.2022
abstract_unstemmed	Optimal sample quality is a prerequisite to generate valid data in the detection of minimal residual disease (MRD) in leukemias. Thus, the risk of obtaining ‘false’-negative results is increased when both quality and quantity of RNA are suboptimal. Factors which affect the sensitivity and consequently the validity of MRD results are reviewed. RNA degradation in unstabilized peripheral blood (PB) samples does not play a major role in samples being processed on the day of blood collection. However, the simulation of sample shipping at room temperature with a delay of sample processing of up to 3 days causes a dramatic loss of intact RNA. RNA degradation can be prevented by the use of a bedside RNA stabilization system. Additionally, the stabilizing procedure is capable of keeping real-time quantitative polymerase chain reaction (RQ-PCR) results comparable whether the sample is processed immediately or with a delay of up to 3 days. Consistent quantitative data cannot be obtained with unstabilized blood samples. Furthermore, the optimum volume of PB required for MRD diagnostics in patients with BCR-ABL-positive chronic myelogenous leukemia in complete cytogenetic remission is revisited. Ten milliliters of PB is sufficient for processing on the day of blood collection whereas the use of only 5 ml PB may result in false-negative results. Standardization of preanalytical and analytical factors is necessary to provide a comparability of RQ-PCR results from different laboratories within multicenter studies. The definition of ‘undetectable BCR-ABL’ in an individual patient should take these preanalytical parameters into consideration. Gesehen am 13.05.2022
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title_short	Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia
url	https://doi.org/10.1159/000077557 https://www.karger.com/Article/FullText/77557
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Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia

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