Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads
Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population c...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kemmner, Wolfram A. - 1964- [verfasserIn] Moldenhauer, Gerhard [verfasserIn] Schlag, Peter M. - 1948- [verfasserIn] Brossmer, Reinhard - 1925-2020 [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
4 March 1992 |
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| Schlagwörter: |
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| Anmerkung: |
Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 Gesehen am 25.06.2024 |
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| Umfang: |
4 |
| Übergeordnetes Werk: |
Enthalten in: Journal of immunological methods - Amsterdam [u.a.] : Elsevier Science, 1971, 147(1992), 2, Seite 197-200 |
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| Übergeordnetes Werk: |
volume:147 ; year:1992 ; number:2 ; pages:197-200 ; extent:4 |
| Links: |
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| DOI / URN: |
10.1016/S0022-1759(12)80008-1 |
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| Katalog-ID: |
1892138239 |
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| 100 | 1 | |a Kemmner, Wolfram A. |d 1964- |e verfasserin |0 (DE-588)173067913 |0 (DE-627)697991989 |0 (DE-576)133920305 |4 aut | |
| 245 | 1 | 0 | |a Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads |c W. Kemmner, G. Moldenhauer, P. Schlag, R. Brossmer |
| 264 | 1 | |c 4 March 1992 | |
| 300 | |a 4 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 | ||
| 500 | |a Gesehen am 25.06.2024 | ||
| 520 | |a Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain. Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead | ||
| 650 | 4 | |a Cell separation | |
| 650 | 4 | |a Colorectal tumor cell | |
| 650 | 4 | |a Immunomagnetic bead | |
| 650 | 4 | |a Monoclonal antibody HEA125, epithelium specific | |
| 650 | 4 | |a Tumor infiltrating leukocyte | |
| 700 | 1 | |a Moldenhauer, Gerhard |e verfasserin |0 (DE-588)1054128634 |0 (DE-627)791055701 |0 (DE-576)410008443 |4 aut | |
| 700 | 1 | |a Schlag, Peter M. |d 1948- |e verfasserin |0 (DE-588)115698965 |0 (DE-627)077414039 |0 (DE-576)170114910 |4 aut | |
| 700 | 1 | |a Brossmer, Reinhard |d 1925-2020 |e verfasserin |0 (DE-588)1115343556 |0 (DE-627)869722298 |0 (DE-576)324407076 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of immunological methods |d Amsterdam [u.a.] : Elsevier Science, 1971 |g 147(1992), 2, Seite 197-200 |h Online-Ressource |w (DE-627)306659751 |w (DE-600)1500495-8 |w (DE-576)081986386 |x 1872-7905 |7 nnns |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/S0022-1759(12)80008-1 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0022175912800081 |x Verlag |z lizenzpflichtig |3 Volltext |
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| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_31 | ||
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| 912 | |a GBV_ILN_39 | ||
| 912 | |a GBV_ILN_40 | ||
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| 912 | |a GBV_ILN_62 | ||
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| 912 | |a GBV_ILN_100 | ||
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| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_121 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
| 912 | |a GBV_ILN_151 | ||
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| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
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| 912 | |a GBV_ILN_213 | ||
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| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_374 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_647 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2018 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2043 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2056 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2158 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2193 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_2807 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4277 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4328 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4346 | ||
| 912 | |a GBV_ILN_4367 | ||
| 912 | |a GBV_ILN_4393 | ||
| 912 | |a GBV_ILN_4700 | ||
| 912 | |a GBV_ILN_4753 | ||
| 951 | |a AR | ||
| 952 | |d 147 |j 1992 |e 2 |h 197-200 |g 4 | ||
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| 982 | |2 2013 |1 01 |x DE-16-250 |8 03 |s s |a s_4 | ||
| 982 | |2 2013 |1 01 |x DE-16-250 |8 04 |s p |0 (DE-627)1516725492 |a Moldenhauer, Gerhard | ||
| 982 | |2 2013 |1 01 |x DE-16-250 |8 04 |s k |0 (DE-627)1416466967 |a Medizinische Fakultät Heidelberg | ||
| 982 | |2 2013 |1 01 |x DE-16-250 |8 04 |s s |0 (DE-627)1410501914 |a Verfasser | ||
| 982 | |2 2013 |1 01 |x DE-16-250 |8 04 |s s |a pos_2 | ||
| 982 | |2 2013 |1 01 |x DE-16-250 |8 05 |s p |0 (DE-627)1892138573 |a Schlag, Peter M. | ||
| 982 | |2 2013 |1 01 |x DE-16-250 |8 05 |s k |0 (DE-627)1416466967 |a Medizinische Fakultät Heidelberg | ||
| 982 | |2 2013 |1 01 |x DE-16-250 |8 05 |s s |0 (DE-627)1410501914 |a Verfasser | ||
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| 982 | |2 2013 |1 01 |x DE-16-250 |8 06 |s p |0 (DE-627)1547733233 |a Brossmer, Reinhard | ||
| 982 | |2 2013 |1 01 |x DE-16-250 |8 06 |s k |0 (DE-627)1416733434 |a Biochemiezentrum der Universität Heidelberg (BZH) | ||
| 982 | |2 2013 |1 01 |x DE-16-250 |8 06 |s s |0 (DE-627)1410501914 |a Verfasser | ||
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4 March 1992 |
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1992 |
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10.1016/S0022-1759(12)80008-1 doi (DE-627)1892138239 (DE-599)KXP1892138239 DE-627 ger DE-627 rda eng Kemmner, Wolfram A. 1964- verfasserin (DE-588)173067913 (DE-627)697991989 (DE-576)133920305 aut Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads W. Kemmner, G. Moldenhauer, P. Schlag, R. Brossmer 4 March 1992 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 Gesehen am 25.06.2024 Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain. Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead Cell separation Colorectal tumor cell Immunomagnetic bead Monoclonal antibody HEA125, epithelium specific Tumor infiltrating leukocyte Moldenhauer, Gerhard verfasserin (DE-588)1054128634 (DE-627)791055701 (DE-576)410008443 aut Schlag, Peter M. 1948- verfasserin (DE-588)115698965 (DE-627)077414039 (DE-576)170114910 aut Brossmer, Reinhard 1925-2020 verfasserin (DE-588)1115343556 (DE-627)869722298 (DE-576)324407076 aut Enthalten in Journal of immunological methods Amsterdam [u.a.] : Elsevier Science, 1971 147(1992), 2, Seite 197-200 Online-Ressource (DE-627)306659751 (DE-600)1500495-8 (DE-576)081986386 1872-7905 nnns volume:147 year:1992 number:2 pages:197-200 extent:4 https://doi.org/10.1016/S0022-1759(12)80008-1 Verlag Resolving-System lizenzpflichtig Volltext https://www.sciencedirect.com/science/article/pii/S0022175912800081 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2043 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2158 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2193 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2807 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 147 1992 2 197-200 4 2013 01 DE-16-250 4541485184 00 --%%-- --%%-- --%%-- --%%-- l01 25-06-24 2013 01 DE-16-250 00 s hd1992 2013 01 DE-16-250 01 (DE-627)1410508463 |s 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_4 2013 01 DE-16-250 04 p (DE-627)1516725492 Moldenhauer, Gerhard 2013 01 DE-16-250 04 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_2 2013 01 DE-16-250 05 p (DE-627)1892138573 Schlag, Peter M. 2013 01 DE-16-250 05 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1547733233 Brossmer, Reinhard 2013 01 DE-16-250 06 k (DE-627)1416733434 Biochemiezentrum der Universität Heidelberg (BZH) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 |
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10.1016/S0022-1759(12)80008-1 doi (DE-627)1892138239 (DE-599)KXP1892138239 DE-627 ger DE-627 rda eng Kemmner, Wolfram A. 1964- verfasserin (DE-588)173067913 (DE-627)697991989 (DE-576)133920305 aut Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads W. Kemmner, G. Moldenhauer, P. Schlag, R. Brossmer 4 March 1992 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 Gesehen am 25.06.2024 Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain. Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead Cell separation Colorectal tumor cell Immunomagnetic bead Monoclonal antibody HEA125, epithelium specific Tumor infiltrating leukocyte Moldenhauer, Gerhard verfasserin (DE-588)1054128634 (DE-627)791055701 (DE-576)410008443 aut Schlag, Peter M. 1948- verfasserin (DE-588)115698965 (DE-627)077414039 (DE-576)170114910 aut Brossmer, Reinhard 1925-2020 verfasserin (DE-588)1115343556 (DE-627)869722298 (DE-576)324407076 aut Enthalten in Journal of immunological methods Amsterdam [u.a.] : Elsevier Science, 1971 147(1992), 2, Seite 197-200 Online-Ressource (DE-627)306659751 (DE-600)1500495-8 (DE-576)081986386 1872-7905 nnns volume:147 year:1992 number:2 pages:197-200 extent:4 https://doi.org/10.1016/S0022-1759(12)80008-1 Verlag Resolving-System lizenzpflichtig Volltext https://www.sciencedirect.com/science/article/pii/S0022175912800081 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2043 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2158 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2193 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2807 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 147 1992 2 197-200 4 2013 01 DE-16-250 4541485184 00 --%%-- --%%-- --%%-- --%%-- l01 25-06-24 2013 01 DE-16-250 00 s hd1992 2013 01 DE-16-250 01 (DE-627)1410508463 |s 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_4 2013 01 DE-16-250 04 p (DE-627)1516725492 Moldenhauer, Gerhard 2013 01 DE-16-250 04 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_2 2013 01 DE-16-250 05 p (DE-627)1892138573 Schlag, Peter M. 2013 01 DE-16-250 05 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1547733233 Brossmer, Reinhard 2013 01 DE-16-250 06 k (DE-627)1416733434 Biochemiezentrum der Universität Heidelberg (BZH) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 |
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10.1016/S0022-1759(12)80008-1 doi (DE-627)1892138239 (DE-599)KXP1892138239 DE-627 ger DE-627 rda eng Kemmner, Wolfram A. 1964- verfasserin (DE-588)173067913 (DE-627)697991989 (DE-576)133920305 aut Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads W. Kemmner, G. Moldenhauer, P. Schlag, R. Brossmer 4 March 1992 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 Gesehen am 25.06.2024 Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain. Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead Cell separation Colorectal tumor cell Immunomagnetic bead Monoclonal antibody HEA125, epithelium specific Tumor infiltrating leukocyte Moldenhauer, Gerhard verfasserin (DE-588)1054128634 (DE-627)791055701 (DE-576)410008443 aut Schlag, Peter M. 1948- verfasserin (DE-588)115698965 (DE-627)077414039 (DE-576)170114910 aut Brossmer, Reinhard 1925-2020 verfasserin (DE-588)1115343556 (DE-627)869722298 (DE-576)324407076 aut Enthalten in Journal of immunological methods Amsterdam [u.a.] : Elsevier Science, 1971 147(1992), 2, Seite 197-200 Online-Ressource (DE-627)306659751 (DE-600)1500495-8 (DE-576)081986386 1872-7905 nnns volume:147 year:1992 number:2 pages:197-200 extent:4 https://doi.org/10.1016/S0022-1759(12)80008-1 Verlag Resolving-System lizenzpflichtig Volltext https://www.sciencedirect.com/science/article/pii/S0022175912800081 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2043 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2158 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2193 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2807 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 147 1992 2 197-200 4 2013 01 DE-16-250 4541485184 00 --%%-- --%%-- --%%-- --%%-- l01 25-06-24 2013 01 DE-16-250 00 s hd1992 2013 01 DE-16-250 01 (DE-627)1410508463 |s 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_4 2013 01 DE-16-250 04 p (DE-627)1516725492 Moldenhauer, Gerhard 2013 01 DE-16-250 04 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_2 2013 01 DE-16-250 05 p (DE-627)1892138573 Schlag, Peter M. 2013 01 DE-16-250 05 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1547733233 Brossmer, Reinhard 2013 01 DE-16-250 06 k (DE-627)1416733434 Biochemiezentrum der Universität Heidelberg (BZH) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 |
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10.1016/S0022-1759(12)80008-1 doi (DE-627)1892138239 (DE-599)KXP1892138239 DE-627 ger DE-627 rda eng Kemmner, Wolfram A. 1964- verfasserin (DE-588)173067913 (DE-627)697991989 (DE-576)133920305 aut Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads W. Kemmner, G. Moldenhauer, P. Schlag, R. Brossmer 4 March 1992 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 Gesehen am 25.06.2024 Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain. Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead Cell separation Colorectal tumor cell Immunomagnetic bead Monoclonal antibody HEA125, epithelium specific Tumor infiltrating leukocyte Moldenhauer, Gerhard verfasserin (DE-588)1054128634 (DE-627)791055701 (DE-576)410008443 aut Schlag, Peter M. 1948- verfasserin (DE-588)115698965 (DE-627)077414039 (DE-576)170114910 aut Brossmer, Reinhard 1925-2020 verfasserin (DE-588)1115343556 (DE-627)869722298 (DE-576)324407076 aut Enthalten in Journal of immunological methods Amsterdam [u.a.] : Elsevier Science, 1971 147(1992), 2, Seite 197-200 Online-Ressource (DE-627)306659751 (DE-600)1500495-8 (DE-576)081986386 1872-7905 nnns volume:147 year:1992 number:2 pages:197-200 extent:4 https://doi.org/10.1016/S0022-1759(12)80008-1 Verlag Resolving-System lizenzpflichtig Volltext https://www.sciencedirect.com/science/article/pii/S0022175912800081 Verlag lizenzpflichtig Volltext GBV_USEFLAG_U GBV_ILN_2013 ISIL_DE-16-250 SYSFLAG_1 GBV_KXP GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2043 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2158 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2193 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2807 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 147 1992 2 197-200 4 2013 01 DE-16-250 4541485184 00 --%%-- --%%-- --%%-- --%%-- l01 25-06-24 2013 01 DE-16-250 00 s hd1992 2013 01 DE-16-250 01 (DE-627)1410508463 |s 2013 01 DE-16-250 02 s per_4 2013 01 DE-16-250 03 s s_4 2013 01 DE-16-250 04 p (DE-627)1516725492 Moldenhauer, Gerhard 2013 01 DE-16-250 04 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 01 DE-16-250 04 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 04 s pos_2 2013 01 DE-16-250 05 p (DE-627)1892138573 Schlag, Peter M. 2013 01 DE-16-250 05 k (DE-627)1416466967 Medizinische Fakultät Heidelberg 2013 01 DE-16-250 05 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 05 s pos_3 2013 01 DE-16-250 06 p (DE-627)1547733233 Brossmer, Reinhard 2013 01 DE-16-250 06 k (DE-627)1416733434 Biochemiezentrum der Universität Heidelberg (BZH) 2013 01 DE-16-250 06 s (DE-627)1410501914 Verfasser 2013 01 DE-16-250 06 s pos_4 |
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Enthalten in Journal of immunological methods 147(1992), 2, Seite 197-200 volume:147 year:1992 number:2 pages:197-200 extent:4 |
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Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell separation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Colorectal tumor cell</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunomagnetic bead</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Monoclonal antibody HEA125, epithelium specific</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Tumor infiltrating leukocyte</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Moldenhauer, Gerhard</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(DE-588)1054128634</subfield><subfield code="0">(DE-627)791055701</subfield><subfield code="0">(DE-576)410008443</subfield><subfield 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separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads |
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Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads |
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Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain. Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 Gesehen am 25.06.2024 |
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Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain. Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 Gesehen am 25.06.2024 |
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Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain. Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 Gesehen am 25.06.2024 |
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