Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva

Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A and HHV6B, their prevalence and clinical significance are limited to isolated publications. Plasma and blood leukocytes were mainly investigated. Saliva is the main factor in the transmission and spread of EBV and HHV6A/B infections, an affordable, inexpensive, non-invasive material for detecting viral DNA. The aim of this work is to improve the methodological base for differential detection of HHV6A/HHV6B and the main types of EBV in saliva. The material of the study was the unstimulated mixed saliva of children aged 1-17 years with acute infectious mononucleosis (n=22) and no clinical symptoms of this disease (n=26), as well as conditionally healthy adults (n=9). Samples were collected once and dynamically (daily for 14 days). The quantitative detection of EBV and HHV6A/В DNA was carried out by the method of polymerase chain reaction (PCR) in real time. For differential detection of EBV1/EBV2 and HHV6A/HHV6В, an optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel was used. As a result, the detection rate of EBV, HHV6A/В and EBV+HHV6A/В DNA in acute infectious mononucleosis was 95%, 91% and 86%, among conventionally healthy children - 69%, 85% and 61.5%, respectively. It was found that among children of the Nizhny Novgorod region, only EBV1 and HHV6B predominate in saliva. According to the results of 14-day dynamic monitoring of saliva virus secretion in healthy virus carriers (adults and children), it was shown that a single EBV DNA study does not allow to reliably assess the infection of individuals or the intensity of EBV secretion. In this case, HHV6A/B is characterized by a more constant and uniform release. The methodological approach optimized in this work makes it possible to separately detect EBV1/EBV2 and HHV6A/HHV6B using a single laboratory protocol, and in combination with an additional stage of saliva sample preparation increases the diagnostic sensitivity of PCR analysis, minimizes the proportion of discordant and false negative results. Such an integrated approach can be applied for diagnostic, epidemiological and research purposes. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Mariia Igorevna Popkova [verfasserIn] Oleg Vladimirovich Utkin [verfasserIn] Daria Alekseevna Bryzgalova [verfasserIn] Anna Olegovna Senatskaia [verfasserIn] Evgeniya Andreevna Soboleva [verfasserIn] Nikolai Aleksandrovich Sakharnov [verfasserIn] Elena Nikolaevna Filatova [verfasserIn] Ekaterina Aleksandrovna Kulova [verfasserIn]

Format:	E-Artikel
Sprache:	Russisch

Erschienen:	2019

Schlagwörter:	ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection.

Übergeordnetes Werk:	In: Инфекция и иммунитет - Sankt-Peterburg : NIIÈM imeni Pastera, 2015, (2019), 0
Übergeordnetes Werk:	year:2019 ; number:0

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ000750824

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allfields	(DE-627)DOAJ000750824 (DE-599)DOAJ769e0c1ea2b542108bc50352d26ee0eb DE-627 ger DE-627 rakwb rus RC109-216 Mariia Igorevna Popkova verfasserin aut Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A and HHV6B, their prevalence and clinical significance are limited to isolated publications. Plasma and blood leukocytes were mainly investigated. Saliva is the main factor in the transmission and spread of EBV and HHV6A/B infections, an affordable, inexpensive, non-invasive material for detecting viral DNA. The aim of this work is to improve the methodological base for differential detection of HHV6A/HHV6B and the main types of EBV in saliva. The material of the study was the unstimulated mixed saliva of children aged 1-17 years with acute infectious mononucleosis (n=22) and no clinical symptoms of this disease (n=26), as well as conditionally healthy adults (n=9). Samples were collected once and dynamically (daily for 14 days). The quantitative detection of EBV and HHV6A/В DNA was carried out by the method of polymerase chain reaction (PCR) in real time. For differential detection of EBV1/EBV2 and HHV6A/HHV6В, an optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel was used. As a result, the detection rate of EBV, HHV6A/В and EBV+HHV6A/В DNA in acute infectious mononucleosis was 95%, 91% and 86%, among conventionally healthy children - 69%, 85% and 61.5%, respectively. It was found that among children of the Nizhny Novgorod region, only EBV1 and HHV6B predominate in saliva. According to the results of 14-day dynamic monitoring of saliva virus secretion in healthy virus carriers (adults and children), it was shown that a single EBV DNA study does not allow to reliably assess the infection of individuals or the intensity of EBV secretion. In this case, HHV6A/B is characterized by a more constant and uniform release. The methodological approach optimized in this work makes it possible to separately detect EBV1/EBV2 and HHV6A/HHV6B using a single laboratory protocol, and in combination with an additional stage of saliva sample preparation increases the diagnostic sensitivity of PCR analysis, minimizes the proportion of discordant and false negative results. Such an integrated approach can be applied for diagnostic, epidemiological and research purposes. ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. Infectious and parasitic diseases Oleg Vladimirovich Utkin verfasserin aut Daria Alekseevna Bryzgalova verfasserin aut Anna Olegovna Senatskaia verfasserin aut Evgeniya Andreevna Soboleva verfasserin aut Nikolai Aleksandrovich Sakharnov verfasserin aut Elena Nikolaevna Filatova verfasserin aut Ekaterina Aleksandrovna Kulova verfasserin aut In Инфекция и иммунитет Sankt-Peterburg : NIIÈM imeni Pastera, 2015 (2019), 0 (DE-627)1740918398 23137398 nnns year:2019 number:0 https://doaj.org/article/769e0c1ea2b542108bc50352d26ee0eb kostenfrei https://www.iimmun.ru/iimm/article/view/1807 kostenfrei https://doaj.org/toc/2220-7619 Journal toc kostenfrei https://doaj.org/toc/2313-7398 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 0
spelling	(DE-627)DOAJ000750824 (DE-599)DOAJ769e0c1ea2b542108bc50352d26ee0eb DE-627 ger DE-627 rakwb rus RC109-216 Mariia Igorevna Popkova verfasserin aut Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A and HHV6B, their prevalence and clinical significance are limited to isolated publications. Plasma and blood leukocytes were mainly investigated. Saliva is the main factor in the transmission and spread of EBV and HHV6A/B infections, an affordable, inexpensive, non-invasive material for detecting viral DNA. The aim of this work is to improve the methodological base for differential detection of HHV6A/HHV6B and the main types of EBV in saliva. The material of the study was the unstimulated mixed saliva of children aged 1-17 years with acute infectious mononucleosis (n=22) and no clinical symptoms of this disease (n=26), as well as conditionally healthy adults (n=9). Samples were collected once and dynamically (daily for 14 days). The quantitative detection of EBV and HHV6A/В DNA was carried out by the method of polymerase chain reaction (PCR) in real time. For differential detection of EBV1/EBV2 and HHV6A/HHV6В, an optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel was used. As a result, the detection rate of EBV, HHV6A/В and EBV+HHV6A/В DNA in acute infectious mononucleosis was 95%, 91% and 86%, among conventionally healthy children - 69%, 85% and 61.5%, respectively. It was found that among children of the Nizhny Novgorod region, only EBV1 and HHV6B predominate in saliva. According to the results of 14-day dynamic monitoring of saliva virus secretion in healthy virus carriers (adults and children), it was shown that a single EBV DNA study does not allow to reliably assess the infection of individuals or the intensity of EBV secretion. In this case, HHV6A/B is characterized by a more constant and uniform release. The methodological approach optimized in this work makes it possible to separately detect EBV1/EBV2 and HHV6A/HHV6B using a single laboratory protocol, and in combination with an additional stage of saliva sample preparation increases the diagnostic sensitivity of PCR analysis, minimizes the proportion of discordant and false negative results. Such an integrated approach can be applied for diagnostic, epidemiological and research purposes. ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. Infectious and parasitic diseases Oleg Vladimirovich Utkin verfasserin aut Daria Alekseevna Bryzgalova verfasserin aut Anna Olegovna Senatskaia verfasserin aut Evgeniya Andreevna Soboleva verfasserin aut Nikolai Aleksandrovich Sakharnov verfasserin aut Elena Nikolaevna Filatova verfasserin aut Ekaterina Aleksandrovna Kulova verfasserin aut In Инфекция и иммунитет Sankt-Peterburg : NIIÈM imeni Pastera, 2015 (2019), 0 (DE-627)1740918398 23137398 nnns year:2019 number:0 https://doaj.org/article/769e0c1ea2b542108bc50352d26ee0eb kostenfrei https://www.iimmun.ru/iimm/article/view/1807 kostenfrei https://doaj.org/toc/2220-7619 Journal toc kostenfrei https://doaj.org/toc/2313-7398 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 0
allfields_unstemmed	(DE-627)DOAJ000750824 (DE-599)DOAJ769e0c1ea2b542108bc50352d26ee0eb DE-627 ger DE-627 rakwb rus RC109-216 Mariia Igorevna Popkova verfasserin aut Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A and HHV6B, their prevalence and clinical significance are limited to isolated publications. Plasma and blood leukocytes were mainly investigated. Saliva is the main factor in the transmission and spread of EBV and HHV6A/B infections, an affordable, inexpensive, non-invasive material for detecting viral DNA. The aim of this work is to improve the methodological base for differential detection of HHV6A/HHV6B and the main types of EBV in saliva. The material of the study was the unstimulated mixed saliva of children aged 1-17 years with acute infectious mononucleosis (n=22) and no clinical symptoms of this disease (n=26), as well as conditionally healthy adults (n=9). Samples were collected once and dynamically (daily for 14 days). The quantitative detection of EBV and HHV6A/В DNA was carried out by the method of polymerase chain reaction (PCR) in real time. For differential detection of EBV1/EBV2 and HHV6A/HHV6В, an optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel was used. As a result, the detection rate of EBV, HHV6A/В and EBV+HHV6A/В DNA in acute infectious mononucleosis was 95%, 91% and 86%, among conventionally healthy children - 69%, 85% and 61.5%, respectively. It was found that among children of the Nizhny Novgorod region, only EBV1 and HHV6B predominate in saliva. According to the results of 14-day dynamic monitoring of saliva virus secretion in healthy virus carriers (adults and children), it was shown that a single EBV DNA study does not allow to reliably assess the infection of individuals or the intensity of EBV secretion. In this case, HHV6A/B is characterized by a more constant and uniform release. The methodological approach optimized in this work makes it possible to separately detect EBV1/EBV2 and HHV6A/HHV6B using a single laboratory protocol, and in combination with an additional stage of saliva sample preparation increases the diagnostic sensitivity of PCR analysis, minimizes the proportion of discordant and false negative results. Such an integrated approach can be applied for diagnostic, epidemiological and research purposes. ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. Infectious and parasitic diseases Oleg Vladimirovich Utkin verfasserin aut Daria Alekseevna Bryzgalova verfasserin aut Anna Olegovna Senatskaia verfasserin aut Evgeniya Andreevna Soboleva verfasserin aut Nikolai Aleksandrovich Sakharnov verfasserin aut Elena Nikolaevna Filatova verfasserin aut Ekaterina Aleksandrovna Kulova verfasserin aut In Инфекция и иммунитет Sankt-Peterburg : NIIÈM imeni Pastera, 2015 (2019), 0 (DE-627)1740918398 23137398 nnns year:2019 number:0 https://doaj.org/article/769e0c1ea2b542108bc50352d26ee0eb kostenfrei https://www.iimmun.ru/iimm/article/view/1807 kostenfrei https://doaj.org/toc/2220-7619 Journal toc kostenfrei https://doaj.org/toc/2313-7398 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 0
allfieldsGer	(DE-627)DOAJ000750824 (DE-599)DOAJ769e0c1ea2b542108bc50352d26ee0eb DE-627 ger DE-627 rakwb rus RC109-216 Mariia Igorevna Popkova verfasserin aut Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A and HHV6B, their prevalence and clinical significance are limited to isolated publications. Plasma and blood leukocytes were mainly investigated. Saliva is the main factor in the transmission and spread of EBV and HHV6A/B infections, an affordable, inexpensive, non-invasive material for detecting viral DNA. The aim of this work is to improve the methodological base for differential detection of HHV6A/HHV6B and the main types of EBV in saliva. The material of the study was the unstimulated mixed saliva of children aged 1-17 years with acute infectious mononucleosis (n=22) and no clinical symptoms of this disease (n=26), as well as conditionally healthy adults (n=9). Samples were collected once and dynamically (daily for 14 days). The quantitative detection of EBV and HHV6A/В DNA was carried out by the method of polymerase chain reaction (PCR) in real time. For differential detection of EBV1/EBV2 and HHV6A/HHV6В, an optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel was used. As a result, the detection rate of EBV, HHV6A/В and EBV+HHV6A/В DNA in acute infectious mononucleosis was 95%, 91% and 86%, among conventionally healthy children - 69%, 85% and 61.5%, respectively. It was found that among children of the Nizhny Novgorod region, only EBV1 and HHV6B predominate in saliva. According to the results of 14-day dynamic monitoring of saliva virus secretion in healthy virus carriers (adults and children), it was shown that a single EBV DNA study does not allow to reliably assess the infection of individuals or the intensity of EBV secretion. In this case, HHV6A/B is characterized by a more constant and uniform release. The methodological approach optimized in this work makes it possible to separately detect EBV1/EBV2 and HHV6A/HHV6B using a single laboratory protocol, and in combination with an additional stage of saliva sample preparation increases the diagnostic sensitivity of PCR analysis, minimizes the proportion of discordant and false negative results. Such an integrated approach can be applied for diagnostic, epidemiological and research purposes. ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. Infectious and parasitic diseases Oleg Vladimirovich Utkin verfasserin aut Daria Alekseevna Bryzgalova verfasserin aut Anna Olegovna Senatskaia verfasserin aut Evgeniya Andreevna Soboleva verfasserin aut Nikolai Aleksandrovich Sakharnov verfasserin aut Elena Nikolaevna Filatova verfasserin aut Ekaterina Aleksandrovna Kulova verfasserin aut In Инфекция и иммунитет Sankt-Peterburg : NIIÈM imeni Pastera, 2015 (2019), 0 (DE-627)1740918398 23137398 nnns year:2019 number:0 https://doaj.org/article/769e0c1ea2b542108bc50352d26ee0eb kostenfrei https://www.iimmun.ru/iimm/article/view/1807 kostenfrei https://doaj.org/toc/2220-7619 Journal toc kostenfrei https://doaj.org/toc/2313-7398 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 0
allfieldsSound	(DE-627)DOAJ000750824 (DE-599)DOAJ769e0c1ea2b542108bc50352d26ee0eb DE-627 ger DE-627 rakwb rus RC109-216 Mariia Igorevna Popkova verfasserin aut Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A and HHV6B, their prevalence and clinical significance are limited to isolated publications. Plasma and blood leukocytes were mainly investigated. Saliva is the main factor in the transmission and spread of EBV and HHV6A/B infections, an affordable, inexpensive, non-invasive material for detecting viral DNA. The aim of this work is to improve the methodological base for differential detection of HHV6A/HHV6B and the main types of EBV in saliva. The material of the study was the unstimulated mixed saliva of children aged 1-17 years with acute infectious mononucleosis (n=22) and no clinical symptoms of this disease (n=26), as well as conditionally healthy adults (n=9). Samples were collected once and dynamically (daily for 14 days). The quantitative detection of EBV and HHV6A/В DNA was carried out by the method of polymerase chain reaction (PCR) in real time. For differential detection of EBV1/EBV2 and HHV6A/HHV6В, an optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel was used. As a result, the detection rate of EBV, HHV6A/В and EBV+HHV6A/В DNA in acute infectious mononucleosis was 95%, 91% and 86%, among conventionally healthy children - 69%, 85% and 61.5%, respectively. It was found that among children of the Nizhny Novgorod region, only EBV1 and HHV6B predominate in saliva. According to the results of 14-day dynamic monitoring of saliva virus secretion in healthy virus carriers (adults and children), it was shown that a single EBV DNA study does not allow to reliably assess the infection of individuals or the intensity of EBV secretion. In this case, HHV6A/B is characterized by a more constant and uniform release. The methodological approach optimized in this work makes it possible to separately detect EBV1/EBV2 and HHV6A/HHV6B using a single laboratory protocol, and in combination with an additional stage of saliva sample preparation increases the diagnostic sensitivity of PCR analysis, minimizes the proportion of discordant and false negative results. Such an integrated approach can be applied for diagnostic, epidemiological and research purposes. ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. Infectious and parasitic diseases Oleg Vladimirovich Utkin verfasserin aut Daria Alekseevna Bryzgalova verfasserin aut Anna Olegovna Senatskaia verfasserin aut Evgeniya Andreevna Soboleva verfasserin aut Nikolai Aleksandrovich Sakharnov verfasserin aut Elena Nikolaevna Filatova verfasserin aut Ekaterina Aleksandrovna Kulova verfasserin aut In Инфекция и иммунитет Sankt-Peterburg : NIIÈM imeni Pastera, 2015 (2019), 0 (DE-627)1740918398 23137398 nnns year:2019 number:0 https://doaj.org/article/769e0c1ea2b542108bc50352d26ee0eb kostenfrei https://www.iimmun.ru/iimm/article/view/1807 kostenfrei https://doaj.org/toc/2220-7619 Journal toc kostenfrei https://doaj.org/toc/2313-7398 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 0
language	Russian
source	In Инфекция и иммунитет (2019), 0 year:2019 number:0
sourceStr	In Инфекция и иммунитет (2019), 0 year:2019 number:0
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. Infectious and parasitic diseases
isfreeaccess_bool	true
container_title	Инфекция и иммунитет
authorswithroles_txt_mv	Mariia Igorevna Popkova @@aut@@ Oleg Vladimirovich Utkin @@aut@@ Daria Alekseevna Bryzgalova @@aut@@ Anna Olegovna Senatskaia @@aut@@ Evgeniya Andreevna Soboleva @@aut@@ Nikolai Aleksandrovich Sakharnov @@aut@@ Elena Nikolaevna Filatova @@aut@@ Ekaterina Aleksandrovna Kulova @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	1740918398
id	DOAJ000750824
language_de	russisch
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callnumber-first	R - Medicine
author	Mariia Igorevna Popkova
spellingShingle	Mariia Igorevna Popkova misc RC109-216 misc ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. misc Infectious and parasitic diseases Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva
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topic_title	RC109-216 Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection
topic	misc RC109-216 misc ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. misc Infectious and parasitic diseases
topic_browse	misc RC109-216 misc ebv1, ebv2, hhv6a, hhv6b, saliva, pcr, genotyping, differential detection. misc Infectious and parasitic diseases
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title	Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva
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title_full	Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva
author_sort	Mariia Igorevna Popkova
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author_browse	Mariia Igorevna Popkova Oleg Vladimirovich Utkin Daria Alekseevna Bryzgalova Anna Olegovna Senatskaia Evgeniya Andreevna Soboleva Nikolai Aleksandrovich Sakharnov Elena Nikolaevna Filatova Ekaterina Aleksandrovna Kulova
class	RC109-216
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author-letter	Mariia Igorevna Popkova
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title_sort	methodological approaches to differential detection of ebv1/ebv2 and hhv6a/hhv6b in saliva
callnumber	RC109-216
title_auth	Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva
abstract	Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A and HHV6B, their prevalence and clinical significance are limited to isolated publications. Plasma and blood leukocytes were mainly investigated. Saliva is the main factor in the transmission and spread of EBV and HHV6A/B infections, an affordable, inexpensive, non-invasive material for detecting viral DNA. The aim of this work is to improve the methodological base for differential detection of HHV6A/HHV6B and the main types of EBV in saliva. The material of the study was the unstimulated mixed saliva of children aged 1-17 years with acute infectious mononucleosis (n=22) and no clinical symptoms of this disease (n=26), as well as conditionally healthy adults (n=9). Samples were collected once and dynamically (daily for 14 days). The quantitative detection of EBV and HHV6A/В DNA was carried out by the method of polymerase chain reaction (PCR) in real time. For differential detection of EBV1/EBV2 and HHV6A/HHV6В, an optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel was used. As a result, the detection rate of EBV, HHV6A/В and EBV+HHV6A/В DNA in acute infectious mononucleosis was 95%, 91% and 86%, among conventionally healthy children - 69%, 85% and 61.5%, respectively. It was found that among children of the Nizhny Novgorod region, only EBV1 and HHV6B predominate in saliva. According to the results of 14-day dynamic monitoring of saliva virus secretion in healthy virus carriers (adults and children), it was shown that a single EBV DNA study does not allow to reliably assess the infection of individuals or the intensity of EBV secretion. In this case, HHV6A/B is characterized by a more constant and uniform release. The methodological approach optimized in this work makes it possible to separately detect EBV1/EBV2 and HHV6A/HHV6B using a single laboratory protocol, and in combination with an additional stage of saliva sample preparation increases the diagnostic sensitivity of PCR analysis, minimizes the proportion of discordant and false negative results. Such an integrated approach can be applied for diagnostic, epidemiological and research purposes.
abstractGer	Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A and HHV6B, their prevalence and clinical significance are limited to isolated publications. Plasma and blood leukocytes were mainly investigated. Saliva is the main factor in the transmission and spread of EBV and HHV6A/B infections, an affordable, inexpensive, non-invasive material for detecting viral DNA. The aim of this work is to improve the methodological base for differential detection of HHV6A/HHV6B and the main types of EBV in saliva. The material of the study was the unstimulated mixed saliva of children aged 1-17 years with acute infectious mononucleosis (n=22) and no clinical symptoms of this disease (n=26), as well as conditionally healthy adults (n=9). Samples were collected once and dynamically (daily for 14 days). The quantitative detection of EBV and HHV6A/В DNA was carried out by the method of polymerase chain reaction (PCR) in real time. For differential detection of EBV1/EBV2 and HHV6A/HHV6В, an optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel was used. As a result, the detection rate of EBV, HHV6A/В and EBV+HHV6A/В DNA in acute infectious mononucleosis was 95%, 91% and 86%, among conventionally healthy children - 69%, 85% and 61.5%, respectively. It was found that among children of the Nizhny Novgorod region, only EBV1 and HHV6B predominate in saliva. According to the results of 14-day dynamic monitoring of saliva virus secretion in healthy virus carriers (adults and children), it was shown that a single EBV DNA study does not allow to reliably assess the infection of individuals or the intensity of EBV secretion. In this case, HHV6A/B is characterized by a more constant and uniform release. The methodological approach optimized in this work makes it possible to separately detect EBV1/EBV2 and HHV6A/HHV6B using a single laboratory protocol, and in combination with an additional stage of saliva sample preparation increases the diagnostic sensitivity of PCR analysis, minimizes the proportion of discordant and false negative results. Such an integrated approach can be applied for diagnostic, epidemiological and research purposes.
abstract_unstemmed	Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A and HHV6B, their prevalence and clinical significance are limited to isolated publications. Plasma and blood leukocytes were mainly investigated. Saliva is the main factor in the transmission and spread of EBV and HHV6A/B infections, an affordable, inexpensive, non-invasive material for detecting viral DNA. The aim of this work is to improve the methodological base for differential detection of HHV6A/HHV6B and the main types of EBV in saliva. The material of the study was the unstimulated mixed saliva of children aged 1-17 years with acute infectious mononucleosis (n=22) and no clinical symptoms of this disease (n=26), as well as conditionally healthy adults (n=9). Samples were collected once and dynamically (daily for 14 days). The quantitative detection of EBV and HHV6A/В DNA was carried out by the method of polymerase chain reaction (PCR) in real time. For differential detection of EBV1/EBV2 and HHV6A/HHV6В, an optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel was used. As a result, the detection rate of EBV, HHV6A/В and EBV+HHV6A/В DNA in acute infectious mononucleosis was 95%, 91% and 86%, among conventionally healthy children - 69%, 85% and 61.5%, respectively. It was found that among children of the Nizhny Novgorod region, only EBV1 and HHV6B predominate in saliva. According to the results of 14-day dynamic monitoring of saliva virus secretion in healthy virus carriers (adults and children), it was shown that a single EBV DNA study does not allow to reliably assess the infection of individuals or the intensity of EBV secretion. In this case, HHV6A/B is characterized by a more constant and uniform release. The methodological approach optimized in this work makes it possible to separately detect EBV1/EBV2 and HHV6A/HHV6B using a single laboratory protocol, and in combination with an additional stage of saliva sample preparation increases the diagnostic sensitivity of PCR analysis, minimizes the proportion of discordant and false negative results. Such an integrated approach can be applied for diagnostic, epidemiological and research purposes.
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title_short	Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva
url	https://doaj.org/article/769e0c1ea2b542108bc50352d26ee0eb https://www.iimmun.ru/iimm/article/view/1807 https://doaj.org/toc/2220-7619 https://doaj.org/toc/2313-7398
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Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva

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