From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use

Antibiotic resistance profiles of <i<Escherichia coli</i< were investigated in an intensive pig production system in the uMgungundlovu District, South Africa, using the ‘farm-to-fork’ approach. Four hundred seventeen (417) samples were collected from pig and pig products at different points (farm, transport, and abattoir). <i<E. coli</i< was isolated and enumerated using the Colilert<sup<®</sup< 18/Quanti-Tray<sup<®</sup< 2000 system. Ten isolates from each Quanti-tray were selected randomly and putatively identified on eosin methylene blue agar. Real-time PCR targeting the <i<uid</i<A gene was used to confirm isolates to the genus level. The Kirby–Bauer disc diffusion method was used to determine the isolates’ antibiotic susceptibility profiles against 20 antibiotics. A total of 1044 confirmed <i<E. coli</i< isolates were obtained across the three critical points in the food chain. Resistance was observed to all the antibiotics tested with the highest and lowest rates obtained against tetracycline (88.5%) and meropenem (0.2%), respectively. Resistance was also observed to chloramphenicol (71.4%), ampicillin (71.1%), trimethoprim-sulfamethoxazole (61.3%), amoxicillin-clavulanate (43.8%), cephalexin (34.3%), azithromycin (23.9%), nalidixic acid (22.1%), cefoxitin (21.1%), ceftriaxone (18.9%), ciprofloxacin (17.3%), cefotaxime (16.9%), gentamicin (15.5%), cefepime (13.8%), ceftazidime (9.8%), amikacin (3.4%), piperacillin-tazobactam (1.2%), tigecycline (0.9%), and imipenem (0.3%). Multidrug resistance (MDR) was observed in 71.2% of the resistant isolates with an overall multiple antibiotic resistance (MAR) index of 0.25, indicating exposure to high antibiotic use environments at the farm level. A high percentage of resistance was observed to growth promoters and antibiotics approved for veterinary medicine in South Africa. Of concern was resistance to critically important antibiotics for animal and human use and the watch and reserve categories of antibiotics. This could have adverse animal and human health consequences from a food safety perspective, necessitating efficient antibiotic stewardship and guidelines to streamline antibiotic use in the food-animal production chain. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Shima E. Abdalla [verfasserIn] Akebe Luther King Abia [verfasserIn] Daniel G. Amoako [verfasserIn] Keith Perrett [verfasserIn] Linda A. Bester [verfasserIn] Sabiha Y. Essack [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	antibiotic susceptibility multidrug resistance multiple-antibiotic resistance index farm-to-fork intensive pig farming

Übergeordnetes Werk:	In: Antibiotics - MDPI AG, 2013, 10(2021), 2, p 178
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:2, p 178

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/antibiotics10020178

Katalog-ID:	DOAJ000923214

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allfields	10.3390/antibiotics10020178 doi (DE-627)DOAJ000923214 (DE-599)DOAJ5debb9cc357949a1bda9a7879c6ac0ef DE-627 ger DE-627 rakwb eng RM1-950 Shima E. Abdalla verfasserin aut From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Antibiotic resistance profiles of <i<Escherichia coli</i< were investigated in an intensive pig production system in the uMgungundlovu District, South Africa, using the ‘farm-to-fork’ approach. Four hundred seventeen (417) samples were collected from pig and pig products at different points (farm, transport, and abattoir). <i<E. coli</i< was isolated and enumerated using the Colilert<sup<®</sup< 18/Quanti-Tray<sup<®</sup< 2000 system. Ten isolates from each Quanti-tray were selected randomly and putatively identified on eosin methylene blue agar. Real-time PCR targeting the <i<uid</i<A gene was used to confirm isolates to the genus level. The Kirby–Bauer disc diffusion method was used to determine the isolates’ antibiotic susceptibility profiles against 20 antibiotics. A total of 1044 confirmed <i<E. coli</i< isolates were obtained across the three critical points in the food chain. Resistance was observed to all the antibiotics tested with the highest and lowest rates obtained against tetracycline (88.5%) and meropenem (0.2%), respectively. Resistance was also observed to chloramphenicol (71.4%), ampicillin (71.1%), trimethoprim-sulfamethoxazole (61.3%), amoxicillin-clavulanate (43.8%), cephalexin (34.3%), azithromycin (23.9%), nalidixic acid (22.1%), cefoxitin (21.1%), ceftriaxone (18.9%), ciprofloxacin (17.3%), cefotaxime (16.9%), gentamicin (15.5%), cefepime (13.8%), ceftazidime (9.8%), amikacin (3.4%), piperacillin-tazobactam (1.2%), tigecycline (0.9%), and imipenem (0.3%). Multidrug resistance (MDR) was observed in 71.2% of the resistant isolates with an overall multiple antibiotic resistance (MAR) index of 0.25, indicating exposure to high antibiotic use environments at the farm level. A high percentage of resistance was observed to growth promoters and antibiotics approved for veterinary medicine in South Africa. Of concern was resistance to critically important antibiotics for animal and human use and the watch and reserve categories of antibiotics. This could have adverse animal and human health consequences from a food safety perspective, necessitating efficient antibiotic stewardship and guidelines to streamline antibiotic use in the food-animal production chain. antibiotic susceptibility <i<E. coli</i< multidrug resistance multiple-antibiotic resistance index farm-to-fork intensive pig farming Therapeutics. Pharmacology Akebe Luther King Abia verfasserin aut Daniel G. Amoako verfasserin aut Keith Perrett verfasserin aut Linda A. Bester verfasserin aut Sabiha Y. Essack verfasserin aut In Antibiotics MDPI AG, 2013 10(2021), 2, p 178 (DE-627)726120596 (DE-600)2681345-2 20796382 nnns volume:10 year:2021 number:2, p 178 https://doi.org/10.3390/antibiotics10020178 kostenfrei https://doaj.org/article/5debb9cc357949a1bda9a7879c6ac0ef kostenfrei https://www.mdpi.com/2079-6382/10/2/178 kostenfrei https://doaj.org/toc/2079-6382 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 2, p 178
spelling	10.3390/antibiotics10020178 doi (DE-627)DOAJ000923214 (DE-599)DOAJ5debb9cc357949a1bda9a7879c6ac0ef DE-627 ger DE-627 rakwb eng RM1-950 Shima E. Abdalla verfasserin aut From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Antibiotic resistance profiles of <i<Escherichia coli</i< were investigated in an intensive pig production system in the uMgungundlovu District, South Africa, using the ‘farm-to-fork’ approach. Four hundred seventeen (417) samples were collected from pig and pig products at different points (farm, transport, and abattoir). <i<E. coli</i< was isolated and enumerated using the Colilert<sup<®</sup< 18/Quanti-Tray<sup<®</sup< 2000 system. Ten isolates from each Quanti-tray were selected randomly and putatively identified on eosin methylene blue agar. Real-time PCR targeting the <i<uid</i<A gene was used to confirm isolates to the genus level. The Kirby–Bauer disc diffusion method was used to determine the isolates’ antibiotic susceptibility profiles against 20 antibiotics. A total of 1044 confirmed <i<E. coli</i< isolates were obtained across the three critical points in the food chain. Resistance was observed to all the antibiotics tested with the highest and lowest rates obtained against tetracycline (88.5%) and meropenem (0.2%), respectively. Resistance was also observed to chloramphenicol (71.4%), ampicillin (71.1%), trimethoprim-sulfamethoxazole (61.3%), amoxicillin-clavulanate (43.8%), cephalexin (34.3%), azithromycin (23.9%), nalidixic acid (22.1%), cefoxitin (21.1%), ceftriaxone (18.9%), ciprofloxacin (17.3%), cefotaxime (16.9%), gentamicin (15.5%), cefepime (13.8%), ceftazidime (9.8%), amikacin (3.4%), piperacillin-tazobactam (1.2%), tigecycline (0.9%), and imipenem (0.3%). Multidrug resistance (MDR) was observed in 71.2% of the resistant isolates with an overall multiple antibiotic resistance (MAR) index of 0.25, indicating exposure to high antibiotic use environments at the farm level. A high percentage of resistance was observed to growth promoters and antibiotics approved for veterinary medicine in South Africa. Of concern was resistance to critically important antibiotics for animal and human use and the watch and reserve categories of antibiotics. This could have adverse animal and human health consequences from a food safety perspective, necessitating efficient antibiotic stewardship and guidelines to streamline antibiotic use in the food-animal production chain. antibiotic susceptibility <i<E. coli</i< multidrug resistance multiple-antibiotic resistance index farm-to-fork intensive pig farming Therapeutics. Pharmacology Akebe Luther King Abia verfasserin aut Daniel G. Amoako verfasserin aut Keith Perrett verfasserin aut Linda A. Bester verfasserin aut Sabiha Y. Essack verfasserin aut In Antibiotics MDPI AG, 2013 10(2021), 2, p 178 (DE-627)726120596 (DE-600)2681345-2 20796382 nnns volume:10 year:2021 number:2, p 178 https://doi.org/10.3390/antibiotics10020178 kostenfrei https://doaj.org/article/5debb9cc357949a1bda9a7879c6ac0ef kostenfrei https://www.mdpi.com/2079-6382/10/2/178 kostenfrei https://doaj.org/toc/2079-6382 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 2, p 178
allfields_unstemmed	10.3390/antibiotics10020178 doi (DE-627)DOAJ000923214 (DE-599)DOAJ5debb9cc357949a1bda9a7879c6ac0ef DE-627 ger DE-627 rakwb eng RM1-950 Shima E. Abdalla verfasserin aut From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Antibiotic resistance profiles of <i<Escherichia coli</i< were investigated in an intensive pig production system in the uMgungundlovu District, South Africa, using the ‘farm-to-fork’ approach. Four hundred seventeen (417) samples were collected from pig and pig products at different points (farm, transport, and abattoir). <i<E. coli</i< was isolated and enumerated using the Colilert<sup<®</sup< 18/Quanti-Tray<sup<®</sup< 2000 system. Ten isolates from each Quanti-tray were selected randomly and putatively identified on eosin methylene blue agar. Real-time PCR targeting the <i<uid</i<A gene was used to confirm isolates to the genus level. The Kirby–Bauer disc diffusion method was used to determine the isolates’ antibiotic susceptibility profiles against 20 antibiotics. A total of 1044 confirmed <i<E. coli</i< isolates were obtained across the three critical points in the food chain. Resistance was observed to all the antibiotics tested with the highest and lowest rates obtained against tetracycline (88.5%) and meropenem (0.2%), respectively. Resistance was also observed to chloramphenicol (71.4%), ampicillin (71.1%), trimethoprim-sulfamethoxazole (61.3%), amoxicillin-clavulanate (43.8%), cephalexin (34.3%), azithromycin (23.9%), nalidixic acid (22.1%), cefoxitin (21.1%), ceftriaxone (18.9%), ciprofloxacin (17.3%), cefotaxime (16.9%), gentamicin (15.5%), cefepime (13.8%), ceftazidime (9.8%), amikacin (3.4%), piperacillin-tazobactam (1.2%), tigecycline (0.9%), and imipenem (0.3%). Multidrug resistance (MDR) was observed in 71.2% of the resistant isolates with an overall multiple antibiotic resistance (MAR) index of 0.25, indicating exposure to high antibiotic use environments at the farm level. A high percentage of resistance was observed to growth promoters and antibiotics approved for veterinary medicine in South Africa. Of concern was resistance to critically important antibiotics for animal and human use and the watch and reserve categories of antibiotics. This could have adverse animal and human health consequences from a food safety perspective, necessitating efficient antibiotic stewardship and guidelines to streamline antibiotic use in the food-animal production chain. antibiotic susceptibility <i<E. coli</i< multidrug resistance multiple-antibiotic resistance index farm-to-fork intensive pig farming Therapeutics. Pharmacology Akebe Luther King Abia verfasserin aut Daniel G. Amoako verfasserin aut Keith Perrett verfasserin aut Linda A. Bester verfasserin aut Sabiha Y. Essack verfasserin aut In Antibiotics MDPI AG, 2013 10(2021), 2, p 178 (DE-627)726120596 (DE-600)2681345-2 20796382 nnns volume:10 year:2021 number:2, p 178 https://doi.org/10.3390/antibiotics10020178 kostenfrei https://doaj.org/article/5debb9cc357949a1bda9a7879c6ac0ef kostenfrei https://www.mdpi.com/2079-6382/10/2/178 kostenfrei https://doaj.org/toc/2079-6382 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 2, p 178
allfieldsGer	10.3390/antibiotics10020178 doi (DE-627)DOAJ000923214 (DE-599)DOAJ5debb9cc357949a1bda9a7879c6ac0ef DE-627 ger DE-627 rakwb eng RM1-950 Shima E. Abdalla verfasserin aut From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Antibiotic resistance profiles of <i<Escherichia coli</i< were investigated in an intensive pig production system in the uMgungundlovu District, South Africa, using the ‘farm-to-fork’ approach. Four hundred seventeen (417) samples were collected from pig and pig products at different points (farm, transport, and abattoir). <i<E. coli</i< was isolated and enumerated using the Colilert<sup<®</sup< 18/Quanti-Tray<sup<®</sup< 2000 system. Ten isolates from each Quanti-tray were selected randomly and putatively identified on eosin methylene blue agar. Real-time PCR targeting the <i<uid</i<A gene was used to confirm isolates to the genus level. The Kirby–Bauer disc diffusion method was used to determine the isolates’ antibiotic susceptibility profiles against 20 antibiotics. A total of 1044 confirmed <i<E. coli</i< isolates were obtained across the three critical points in the food chain. Resistance was observed to all the antibiotics tested with the highest and lowest rates obtained against tetracycline (88.5%) and meropenem (0.2%), respectively. Resistance was also observed to chloramphenicol (71.4%), ampicillin (71.1%), trimethoprim-sulfamethoxazole (61.3%), amoxicillin-clavulanate (43.8%), cephalexin (34.3%), azithromycin (23.9%), nalidixic acid (22.1%), cefoxitin (21.1%), ceftriaxone (18.9%), ciprofloxacin (17.3%), cefotaxime (16.9%), gentamicin (15.5%), cefepime (13.8%), ceftazidime (9.8%), amikacin (3.4%), piperacillin-tazobactam (1.2%), tigecycline (0.9%), and imipenem (0.3%). Multidrug resistance (MDR) was observed in 71.2% of the resistant isolates with an overall multiple antibiotic resistance (MAR) index of 0.25, indicating exposure to high antibiotic use environments at the farm level. A high percentage of resistance was observed to growth promoters and antibiotics approved for veterinary medicine in South Africa. Of concern was resistance to critically important antibiotics for animal and human use and the watch and reserve categories of antibiotics. This could have adverse animal and human health consequences from a food safety perspective, necessitating efficient antibiotic stewardship and guidelines to streamline antibiotic use in the food-animal production chain. antibiotic susceptibility <i<E. coli</i< multidrug resistance multiple-antibiotic resistance index farm-to-fork intensive pig farming Therapeutics. Pharmacology Akebe Luther King Abia verfasserin aut Daniel G. Amoako verfasserin aut Keith Perrett verfasserin aut Linda A. Bester verfasserin aut Sabiha Y. Essack verfasserin aut In Antibiotics MDPI AG, 2013 10(2021), 2, p 178 (DE-627)726120596 (DE-600)2681345-2 20796382 nnns volume:10 year:2021 number:2, p 178 https://doi.org/10.3390/antibiotics10020178 kostenfrei https://doaj.org/article/5debb9cc357949a1bda9a7879c6ac0ef kostenfrei https://www.mdpi.com/2079-6382/10/2/178 kostenfrei https://doaj.org/toc/2079-6382 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 2, p 178
allfieldsSound	10.3390/antibiotics10020178 doi (DE-627)DOAJ000923214 (DE-599)DOAJ5debb9cc357949a1bda9a7879c6ac0ef DE-627 ger DE-627 rakwb eng RM1-950 Shima E. Abdalla verfasserin aut From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Antibiotic resistance profiles of <i<Escherichia coli</i< were investigated in an intensive pig production system in the uMgungundlovu District, South Africa, using the ‘farm-to-fork’ approach. Four hundred seventeen (417) samples were collected from pig and pig products at different points (farm, transport, and abattoir). <i<E. coli</i< was isolated and enumerated using the Colilert<sup<®</sup< 18/Quanti-Tray<sup<®</sup< 2000 system. Ten isolates from each Quanti-tray were selected randomly and putatively identified on eosin methylene blue agar. Real-time PCR targeting the <i<uid</i<A gene was used to confirm isolates to the genus level. The Kirby–Bauer disc diffusion method was used to determine the isolates’ antibiotic susceptibility profiles against 20 antibiotics. A total of 1044 confirmed <i<E. coli</i< isolates were obtained across the three critical points in the food chain. Resistance was observed to all the antibiotics tested with the highest and lowest rates obtained against tetracycline (88.5%) and meropenem (0.2%), respectively. Resistance was also observed to chloramphenicol (71.4%), ampicillin (71.1%), trimethoprim-sulfamethoxazole (61.3%), amoxicillin-clavulanate (43.8%), cephalexin (34.3%), azithromycin (23.9%), nalidixic acid (22.1%), cefoxitin (21.1%), ceftriaxone (18.9%), ciprofloxacin (17.3%), cefotaxime (16.9%), gentamicin (15.5%), cefepime (13.8%), ceftazidime (9.8%), amikacin (3.4%), piperacillin-tazobactam (1.2%), tigecycline (0.9%), and imipenem (0.3%). Multidrug resistance (MDR) was observed in 71.2% of the resistant isolates with an overall multiple antibiotic resistance (MAR) index of 0.25, indicating exposure to high antibiotic use environments at the farm level. A high percentage of resistance was observed to growth promoters and antibiotics approved for veterinary medicine in South Africa. Of concern was resistance to critically important antibiotics for animal and human use and the watch and reserve categories of antibiotics. This could have adverse animal and human health consequences from a food safety perspective, necessitating efficient antibiotic stewardship and guidelines to streamline antibiotic use in the food-animal production chain. antibiotic susceptibility <i<E. coli</i< multidrug resistance multiple-antibiotic resistance index farm-to-fork intensive pig farming Therapeutics. Pharmacology Akebe Luther King Abia verfasserin aut Daniel G. Amoako verfasserin aut Keith Perrett verfasserin aut Linda A. Bester verfasserin aut Sabiha Y. Essack verfasserin aut In Antibiotics MDPI AG, 2013 10(2021), 2, p 178 (DE-627)726120596 (DE-600)2681345-2 20796382 nnns volume:10 year:2021 number:2, p 178 https://doi.org/10.3390/antibiotics10020178 kostenfrei https://doaj.org/article/5debb9cc357949a1bda9a7879c6ac0ef kostenfrei https://www.mdpi.com/2079-6382/10/2/178 kostenfrei https://doaj.org/toc/2079-6382 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 2, p 178
language	English
source	In Antibiotics 10(2021), 2, p 178 volume:10 year:2021 number:2, p 178
sourceStr	In Antibiotics 10(2021), 2, p 178 volume:10 year:2021 number:2, p 178
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	antibiotic susceptibility <i<E. coli</i< multidrug resistance multiple-antibiotic resistance index farm-to-fork intensive pig farming Therapeutics. Pharmacology
isfreeaccess_bool	true
container_title	Antibiotics
authorswithroles_txt_mv	Shima E. Abdalla @@aut@@ Akebe Luther King Abia @@aut@@ Daniel G. Amoako @@aut@@ Keith Perrett @@aut@@ Linda A. Bester @@aut@@ Sabiha Y. Essack @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	726120596
id	DOAJ000923214
language_de	englisch
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callnumber-first	R - Medicine
author	Shima E. Abdalla
spellingShingle	Shima E. Abdalla misc RM1-950 misc antibiotic susceptibility misc <i<E. coli</i< misc multidrug resistance misc multiple-antibiotic resistance index misc farm-to-fork misc intensive pig farming misc Therapeutics. Pharmacology From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use
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topic_title	RM1-950 From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use antibiotic susceptibility <i<E. coli</i< multidrug resistance multiple-antibiotic resistance index farm-to-fork intensive pig farming
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title	From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use
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title_full	From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use
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author_browse	Shima E. Abdalla Akebe Luther King Abia Daniel G. Amoako Keith Perrett Linda A. Bester Sabiha Y. Essack
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title_sort	from farm-to-fork: <i<e. coli</i< from an intensive pig production system in south africa shows high resistance to critically important antibiotics for human and animal use
callnumber	RM1-950
title_auth	From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use
abstract	Antibiotic resistance profiles of <i<Escherichia coli</i< were investigated in an intensive pig production system in the uMgungundlovu District, South Africa, using the ‘farm-to-fork’ approach. Four hundred seventeen (417) samples were collected from pig and pig products at different points (farm, transport, and abattoir). <i<E. coli</i< was isolated and enumerated using the Colilert<sup<®</sup< 18/Quanti-Tray<sup<®</sup< 2000 system. Ten isolates from each Quanti-tray were selected randomly and putatively identified on eosin methylene blue agar. Real-time PCR targeting the <i<uid</i<A gene was used to confirm isolates to the genus level. The Kirby–Bauer disc diffusion method was used to determine the isolates’ antibiotic susceptibility profiles against 20 antibiotics. A total of 1044 confirmed <i<E. coli</i< isolates were obtained across the three critical points in the food chain. Resistance was observed to all the antibiotics tested with the highest and lowest rates obtained against tetracycline (88.5%) and meropenem (0.2%), respectively. Resistance was also observed to chloramphenicol (71.4%), ampicillin (71.1%), trimethoprim-sulfamethoxazole (61.3%), amoxicillin-clavulanate (43.8%), cephalexin (34.3%), azithromycin (23.9%), nalidixic acid (22.1%), cefoxitin (21.1%), ceftriaxone (18.9%), ciprofloxacin (17.3%), cefotaxime (16.9%), gentamicin (15.5%), cefepime (13.8%), ceftazidime (9.8%), amikacin (3.4%), piperacillin-tazobactam (1.2%), tigecycline (0.9%), and imipenem (0.3%). Multidrug resistance (MDR) was observed in 71.2% of the resistant isolates with an overall multiple antibiotic resistance (MAR) index of 0.25, indicating exposure to high antibiotic use environments at the farm level. A high percentage of resistance was observed to growth promoters and antibiotics approved for veterinary medicine in South Africa. Of concern was resistance to critically important antibiotics for animal and human use and the watch and reserve categories of antibiotics. This could have adverse animal and human health consequences from a food safety perspective, necessitating efficient antibiotic stewardship and guidelines to streamline antibiotic use in the food-animal production chain.
abstractGer	Antibiotic resistance profiles of <i<Escherichia coli</i< were investigated in an intensive pig production system in the uMgungundlovu District, South Africa, using the ‘farm-to-fork’ approach. Four hundred seventeen (417) samples were collected from pig and pig products at different points (farm, transport, and abattoir). <i<E. coli</i< was isolated and enumerated using the Colilert<sup<®</sup< 18/Quanti-Tray<sup<®</sup< 2000 system. Ten isolates from each Quanti-tray were selected randomly and putatively identified on eosin methylene blue agar. Real-time PCR targeting the <i<uid</i<A gene was used to confirm isolates to the genus level. The Kirby–Bauer disc diffusion method was used to determine the isolates’ antibiotic susceptibility profiles against 20 antibiotics. A total of 1044 confirmed <i<E. coli</i< isolates were obtained across the three critical points in the food chain. Resistance was observed to all the antibiotics tested with the highest and lowest rates obtained against tetracycline (88.5%) and meropenem (0.2%), respectively. Resistance was also observed to chloramphenicol (71.4%), ampicillin (71.1%), trimethoprim-sulfamethoxazole (61.3%), amoxicillin-clavulanate (43.8%), cephalexin (34.3%), azithromycin (23.9%), nalidixic acid (22.1%), cefoxitin (21.1%), ceftriaxone (18.9%), ciprofloxacin (17.3%), cefotaxime (16.9%), gentamicin (15.5%), cefepime (13.8%), ceftazidime (9.8%), amikacin (3.4%), piperacillin-tazobactam (1.2%), tigecycline (0.9%), and imipenem (0.3%). Multidrug resistance (MDR) was observed in 71.2% of the resistant isolates with an overall multiple antibiotic resistance (MAR) index of 0.25, indicating exposure to high antibiotic use environments at the farm level. A high percentage of resistance was observed to growth promoters and antibiotics approved for veterinary medicine in South Africa. Of concern was resistance to critically important antibiotics for animal and human use and the watch and reserve categories of antibiotics. This could have adverse animal and human health consequences from a food safety perspective, necessitating efficient antibiotic stewardship and guidelines to streamline antibiotic use in the food-animal production chain.
abstract_unstemmed	Antibiotic resistance profiles of <i<Escherichia coli</i< were investigated in an intensive pig production system in the uMgungundlovu District, South Africa, using the ‘farm-to-fork’ approach. Four hundred seventeen (417) samples were collected from pig and pig products at different points (farm, transport, and abattoir). <i<E. coli</i< was isolated and enumerated using the Colilert<sup<®</sup< 18/Quanti-Tray<sup<®</sup< 2000 system. Ten isolates from each Quanti-tray were selected randomly and putatively identified on eosin methylene blue agar. Real-time PCR targeting the <i<uid</i<A gene was used to confirm isolates to the genus level. The Kirby–Bauer disc diffusion method was used to determine the isolates’ antibiotic susceptibility profiles against 20 antibiotics. A total of 1044 confirmed <i<E. coli</i< isolates were obtained across the three critical points in the food chain. Resistance was observed to all the antibiotics tested with the highest and lowest rates obtained against tetracycline (88.5%) and meropenem (0.2%), respectively. Resistance was also observed to chloramphenicol (71.4%), ampicillin (71.1%), trimethoprim-sulfamethoxazole (61.3%), amoxicillin-clavulanate (43.8%), cephalexin (34.3%), azithromycin (23.9%), nalidixic acid (22.1%), cefoxitin (21.1%), ceftriaxone (18.9%), ciprofloxacin (17.3%), cefotaxime (16.9%), gentamicin (15.5%), cefepime (13.8%), ceftazidime (9.8%), amikacin (3.4%), piperacillin-tazobactam (1.2%), tigecycline (0.9%), and imipenem (0.3%). Multidrug resistance (MDR) was observed in 71.2% of the resistant isolates with an overall multiple antibiotic resistance (MAR) index of 0.25, indicating exposure to high antibiotic use environments at the farm level. A high percentage of resistance was observed to growth promoters and antibiotics approved for veterinary medicine in South Africa. Of concern was resistance to critically important antibiotics for animal and human use and the watch and reserve categories of antibiotics. This could have adverse animal and human health consequences from a food safety perspective, necessitating efficient antibiotic stewardship and guidelines to streamline antibiotic use in the food-animal production chain.
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title_short	From Farm-to-Fork: <i<E. Coli</i< from an Intensive Pig Production System in South Africa Shows High Resistance to Critically Important Antibiotics for Human and Animal Use
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