Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier

Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additi...
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Autor*in:	Alba García-Rodríguez [verfasserIn] Laura Vila [verfasserIn] Constanza Cortés [verfasserIn] Alba Hernández [verfasserIn] Ricard Marcos [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	TiO2NPs nanospheres TiO2NPs nanorods TiO2NPs nanowires Intestinal barrier In vitro

Übergeordnetes Werk:	In: Particle and Fibre Toxicology - BMC, 2004, 15(2018), 1, Seite 16
Übergeordnetes Werk:	volume:15 ; year:2018 ; number:1 ; pages:16

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12989-018-0269-x

Katalog-ID:	DOAJ001041762

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520			\|a Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additive, demands a deep assessment of their potential risk for humans, including their abilities to cross biological barriers. Methods We have investigated the interaction of three differently shaped TiO2NPs (nanospheres, nanorods and nanowires) in an in vitro model of the intestinal barrier, where the coculture of Caco-2/HT29 cells confers inherent intestinal epithelium characteristics to the model (i.e. mucus secretion, brush border, tight junctions, etc.). Results Adverse effects in the intestinal epithelium were detected by studying the barrier’s integrity (TEER), permeability (LY) and changes in the gene expression of selected specific markers. Using Laser Scanning Confocal Microscopy, we detected a different behaviour in the bio-adhesion and biodistribution of each of the TiO2NPs. Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects.
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allfields	10.1186/s12989-018-0269-x doi (DE-627)DOAJ001041762 (DE-599)DOAJdfe0e236486f4267af57fd862c7eaab9 DE-627 ger DE-627 rakwb eng RA1190-1270 HD7260-7780.8 Alba García-Rodríguez verfasserin aut Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additive, demands a deep assessment of their potential risk for humans, including their abilities to cross biological barriers. Methods We have investigated the interaction of three differently shaped TiO2NPs (nanospheres, nanorods and nanowires) in an in vitro model of the intestinal barrier, where the coculture of Caco-2/HT29 cells confers inherent intestinal epithelium characteristics to the model (i.e. mucus secretion, brush border, tight junctions, etc.). Results Adverse effects in the intestinal epithelium were detected by studying the barrier’s integrity (TEER), permeability (LY) and changes in the gene expression of selected specific markers. Using Laser Scanning Confocal Microscopy, we detected a different behaviour in the bio-adhesion and biodistribution of each of the TiO2NPs. Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects. TiO2NPs nanospheres TiO2NPs nanorods TiO2NPs nanowires Intestinal barrier In vitro Toxicology. Poisons Industrial hygiene. Industrial welfare Laura Vila verfasserin aut Constanza Cortés verfasserin aut Alba Hernández verfasserin aut Ricard Marcos verfasserin aut In Particle and Fibre Toxicology BMC, 2004 15(2018), 1, Seite 16 (DE-627)474928276 (DE-600)2170936-1 17438977 nnns volume:15 year:2018 number:1 pages:16 https://doi.org/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/article/dfe0e236486f4267af57fd862c7eaab9 kostenfrei http://link.springer.com/article/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/toc/1743-8977 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 16
spelling	10.1186/s12989-018-0269-x doi (DE-627)DOAJ001041762 (DE-599)DOAJdfe0e236486f4267af57fd862c7eaab9 DE-627 ger DE-627 rakwb eng RA1190-1270 HD7260-7780.8 Alba García-Rodríguez verfasserin aut Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additive, demands a deep assessment of their potential risk for humans, including their abilities to cross biological barriers. Methods We have investigated the interaction of three differently shaped TiO2NPs (nanospheres, nanorods and nanowires) in an in vitro model of the intestinal barrier, where the coculture of Caco-2/HT29 cells confers inherent intestinal epithelium characteristics to the model (i.e. mucus secretion, brush border, tight junctions, etc.). Results Adverse effects in the intestinal epithelium were detected by studying the barrier’s integrity (TEER), permeability (LY) and changes in the gene expression of selected specific markers. Using Laser Scanning Confocal Microscopy, we detected a different behaviour in the bio-adhesion and biodistribution of each of the TiO2NPs. Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects. TiO2NPs nanospheres TiO2NPs nanorods TiO2NPs nanowires Intestinal barrier In vitro Toxicology. Poisons Industrial hygiene. Industrial welfare Laura Vila verfasserin aut Constanza Cortés verfasserin aut Alba Hernández verfasserin aut Ricard Marcos verfasserin aut In Particle and Fibre Toxicology BMC, 2004 15(2018), 1, Seite 16 (DE-627)474928276 (DE-600)2170936-1 17438977 nnns volume:15 year:2018 number:1 pages:16 https://doi.org/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/article/dfe0e236486f4267af57fd862c7eaab9 kostenfrei http://link.springer.com/article/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/toc/1743-8977 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 16
allfields_unstemmed	10.1186/s12989-018-0269-x doi (DE-627)DOAJ001041762 (DE-599)DOAJdfe0e236486f4267af57fd862c7eaab9 DE-627 ger DE-627 rakwb eng RA1190-1270 HD7260-7780.8 Alba García-Rodríguez verfasserin aut Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additive, demands a deep assessment of their potential risk for humans, including their abilities to cross biological barriers. Methods We have investigated the interaction of three differently shaped TiO2NPs (nanospheres, nanorods and nanowires) in an in vitro model of the intestinal barrier, where the coculture of Caco-2/HT29 cells confers inherent intestinal epithelium characteristics to the model (i.e. mucus secretion, brush border, tight junctions, etc.). Results Adverse effects in the intestinal epithelium were detected by studying the barrier’s integrity (TEER), permeability (LY) and changes in the gene expression of selected specific markers. Using Laser Scanning Confocal Microscopy, we detected a different behaviour in the bio-adhesion and biodistribution of each of the TiO2NPs. Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects. TiO2NPs nanospheres TiO2NPs nanorods TiO2NPs nanowires Intestinal barrier In vitro Toxicology. Poisons Industrial hygiene. Industrial welfare Laura Vila verfasserin aut Constanza Cortés verfasserin aut Alba Hernández verfasserin aut Ricard Marcos verfasserin aut In Particle and Fibre Toxicology BMC, 2004 15(2018), 1, Seite 16 (DE-627)474928276 (DE-600)2170936-1 17438977 nnns volume:15 year:2018 number:1 pages:16 https://doi.org/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/article/dfe0e236486f4267af57fd862c7eaab9 kostenfrei http://link.springer.com/article/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/toc/1743-8977 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 16
allfieldsGer	10.1186/s12989-018-0269-x doi (DE-627)DOAJ001041762 (DE-599)DOAJdfe0e236486f4267af57fd862c7eaab9 DE-627 ger DE-627 rakwb eng RA1190-1270 HD7260-7780.8 Alba García-Rodríguez verfasserin aut Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additive, demands a deep assessment of their potential risk for humans, including their abilities to cross biological barriers. Methods We have investigated the interaction of three differently shaped TiO2NPs (nanospheres, nanorods and nanowires) in an in vitro model of the intestinal barrier, where the coculture of Caco-2/HT29 cells confers inherent intestinal epithelium characteristics to the model (i.e. mucus secretion, brush border, tight junctions, etc.). Results Adverse effects in the intestinal epithelium were detected by studying the barrier’s integrity (TEER), permeability (LY) and changes in the gene expression of selected specific markers. Using Laser Scanning Confocal Microscopy, we detected a different behaviour in the bio-adhesion and biodistribution of each of the TiO2NPs. Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects. TiO2NPs nanospheres TiO2NPs nanorods TiO2NPs nanowires Intestinal barrier In vitro Toxicology. Poisons Industrial hygiene. Industrial welfare Laura Vila verfasserin aut Constanza Cortés verfasserin aut Alba Hernández verfasserin aut Ricard Marcos verfasserin aut In Particle and Fibre Toxicology BMC, 2004 15(2018), 1, Seite 16 (DE-627)474928276 (DE-600)2170936-1 17438977 nnns volume:15 year:2018 number:1 pages:16 https://doi.org/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/article/dfe0e236486f4267af57fd862c7eaab9 kostenfrei http://link.springer.com/article/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/toc/1743-8977 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 16
allfieldsSound	10.1186/s12989-018-0269-x doi (DE-627)DOAJ001041762 (DE-599)DOAJdfe0e236486f4267af57fd862c7eaab9 DE-627 ger DE-627 rakwb eng RA1190-1270 HD7260-7780.8 Alba García-Rodríguez verfasserin aut Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additive, demands a deep assessment of their potential risk for humans, including their abilities to cross biological barriers. Methods We have investigated the interaction of three differently shaped TiO2NPs (nanospheres, nanorods and nanowires) in an in vitro model of the intestinal barrier, where the coculture of Caco-2/HT29 cells confers inherent intestinal epithelium characteristics to the model (i.e. mucus secretion, brush border, tight junctions, etc.). Results Adverse effects in the intestinal epithelium were detected by studying the barrier’s integrity (TEER), permeability (LY) and changes in the gene expression of selected specific markers. Using Laser Scanning Confocal Microscopy, we detected a different behaviour in the bio-adhesion and biodistribution of each of the TiO2NPs. Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects. TiO2NPs nanospheres TiO2NPs nanorods TiO2NPs nanowires Intestinal barrier In vitro Toxicology. Poisons Industrial hygiene. Industrial welfare Laura Vila verfasserin aut Constanza Cortés verfasserin aut Alba Hernández verfasserin aut Ricard Marcos verfasserin aut In Particle and Fibre Toxicology BMC, 2004 15(2018), 1, Seite 16 (DE-627)474928276 (DE-600)2170936-1 17438977 nnns volume:15 year:2018 number:1 pages:16 https://doi.org/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/article/dfe0e236486f4267af57fd862c7eaab9 kostenfrei http://link.springer.com/article/10.1186/s12989-018-0269-x kostenfrei https://doaj.org/toc/1743-8977 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2018 1 16
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callnumber-first	R - Medicine
author	Alba García-Rodríguez
spellingShingle	Alba García-Rodríguez misc RA1190-1270 misc HD7260-7780.8 misc TiO2NPs nanospheres misc TiO2NPs nanorods misc TiO2NPs nanowires misc Intestinal barrier misc In vitro misc Toxicology. Poisons misc Industrial hygiene. Industrial welfare Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier
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topic_title	RA1190-1270 HD7260-7780.8 Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier TiO2NPs nanospheres TiO2NPs nanorods TiO2NPs nanowires Intestinal barrier In vitro
topic	misc RA1190-1270 misc HD7260-7780.8 misc TiO2NPs nanospheres misc TiO2NPs nanorods misc TiO2NPs nanowires misc Intestinal barrier misc In vitro misc Toxicology. Poisons misc Industrial hygiene. Industrial welfare
topic_unstemmed	misc RA1190-1270 misc HD7260-7780.8 misc TiO2NPs nanospheres misc TiO2NPs nanorods misc TiO2NPs nanowires misc Intestinal barrier misc In vitro misc Toxicology. Poisons misc Industrial hygiene. Industrial welfare
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title	Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier
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title_full	Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier
author_sort	Alba García-Rodríguez
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author_browse	Alba García-Rodríguez Laura Vila Constanza Cortés Alba Hernández Ricard Marcos
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title_sort	effects of differently shaped tio2nps (nanospheres, nanorods and nanowires) on the in vitro model (caco-2/ht29) of the intestinal barrier
callnumber	RA1190-1270
title_auth	Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier
abstract	Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additive, demands a deep assessment of their potential risk for humans, including their abilities to cross biological barriers. Methods We have investigated the interaction of three differently shaped TiO2NPs (nanospheres, nanorods and nanowires) in an in vitro model of the intestinal barrier, where the coculture of Caco-2/HT29 cells confers inherent intestinal epithelium characteristics to the model (i.e. mucus secretion, brush border, tight junctions, etc.). Results Adverse effects in the intestinal epithelium were detected by studying the barrier’s integrity (TEER), permeability (LY) and changes in the gene expression of selected specific markers. Using Laser Scanning Confocal Microscopy, we detected a different behaviour in the bio-adhesion and biodistribution of each of the TiO2NPs. Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects.
abstractGer	Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additive, demands a deep assessment of their potential risk for humans, including their abilities to cross biological barriers. Methods We have investigated the interaction of three differently shaped TiO2NPs (nanospheres, nanorods and nanowires) in an in vitro model of the intestinal barrier, where the coculture of Caco-2/HT29 cells confers inherent intestinal epithelium characteristics to the model (i.e. mucus secretion, brush border, tight junctions, etc.). Results Adverse effects in the intestinal epithelium were detected by studying the barrier’s integrity (TEER), permeability (LY) and changes in the gene expression of selected specific markers. Using Laser Scanning Confocal Microscopy, we detected a different behaviour in the bio-adhesion and biodistribution of each of the TiO2NPs. Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects.
abstract_unstemmed	Abstract Background The biological effects of nanoparticles depend on several characteristics such as size and shape that must be taken into account in any type of assessment. The increased use of titanium dioxide nanoparticles (TiO2NPs) for industrial applications, and specifically as a food additive, demands a deep assessment of their potential risk for humans, including their abilities to cross biological barriers. Methods We have investigated the interaction of three differently shaped TiO2NPs (nanospheres, nanorods and nanowires) in an in vitro model of the intestinal barrier, where the coculture of Caco-2/HT29 cells confers inherent intestinal epithelium characteristics to the model (i.e. mucus secretion, brush border, tight junctions, etc.). Results Adverse effects in the intestinal epithelium were detected by studying the barrier’s integrity (TEER), permeability (LY) and changes in the gene expression of selected specific markers. Using Laser Scanning Confocal Microscopy, we detected a different behaviour in the bio-adhesion and biodistribution of each of the TiO2NPs. Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects.
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title_short	Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier
url	https://doi.org/10.1186/s12989-018-0269-x https://doaj.org/article/dfe0e236486f4267af57fd862c7eaab9 http://link.springer.com/article/10.1186/s12989-018-0269-x https://doaj.org/toc/1743-8977
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author2	Laura Vila Constanza Cortés Alba Hernández Ricard Marcos
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up_date	2024-07-03T18:03:03.539Z
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Moreover, we were able to specifically localize each type of TiO2NPs inside the cells. Interestingly, general DNA damage, but not oxidative DNA damage effects, were detected by using the FPG version of the comet assay. Conclusions Results indicate different interactions and cellular responses related to differently shaped TiO2NPs, nanowires showing the most harmful effects.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">TiO2NPs nanospheres</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">TiO2NPs nanorods</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">TiO2NPs nanowires</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Intestinal barrier</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">In vitro</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Toxicology. 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Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier

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