Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes
Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabete...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Emmanuel Anyachukwu Irondi [verfasserIn] Ganiyu Oboh [verfasserIn] Afolabi Akintunde Akindahunsi [verfasserIn] Aline Augusti Boligon [verfasserIn] Margareth Linde Athayde [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Asian Pacific Journal of Tropical Biomedicine - Wolters Kluwer Medknow Publications, 2016, 4(2014), 11, Seite 903-910 |
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| Übergeordnetes Werk: |
volume:4 ; year:2014 ; number:11 ; pages:903-910 |
| Links: |
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| DOI / URN: |
10.12980/APJTB.4.201414B364 |
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| Katalog-ID: |
DOAJ001388959 |
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| 245 | 1 | 0 | |a Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes |
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| 520 | |a Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. | ||
| 650 | 4 | |a Hyperglycemia | |
| 650 | 4 | |a Diabetic complications | |
| 650 | 4 | |a Enzyme inhibition | |
| 650 | 4 | |a Lipid peroxidation | |
| 650 | 4 | |a Mangifera indica | |
| 650 | 4 | |a Mucuna urens | |
| 653 | 0 | |a Arctic medicine. Tropical medicine | |
| 653 | 0 | |a Biology (General) | |
| 700 | 0 | |a Ganiyu Oboh |e verfasserin |4 aut | |
| 700 | 0 | |a Afolabi Akintunde Akindahunsi |e verfasserin |4 aut | |
| 700 | 0 | |a Aline Augusti Boligon |e verfasserin |4 aut | |
| 700 | 0 | |a Margareth Linde Athayde |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:4 |g year:2014 |g number:11 |g pages:903-910 |
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| 856 | 4 | 0 | |u https://doaj.org/article/44eb13c2d74641edbc5bdc0f7324fcd3 |z kostenfrei |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S2221169115300381 |z kostenfrei |
| 856 | 4 | 2 | |u https://doaj.org/toc/2221-1691 |y Journal toc |z kostenfrei |
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10.12980/APJTB.4.201414B364 doi (DE-627)DOAJ001388959 (DE-599)DOAJ44eb13c2d74641edbc5bdc0f7324fcd3 DE-627 ger DE-627 rakwb eng RC955-962 QH301-705.5 Emmanuel Anyachukwu Irondi verfasserin aut Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. Hyperglycemia Diabetic complications Enzyme inhibition Lipid peroxidation Mangifera indica Mucuna urens Arctic medicine. Tropical medicine Biology (General) Ganiyu Oboh verfasserin aut Afolabi Akintunde Akindahunsi verfasserin aut Aline Augusti Boligon verfasserin aut Margareth Linde Athayde verfasserin aut In Asian Pacific Journal of Tropical Biomedicine Wolters Kluwer Medknow Publications, 2016 4(2014), 11, Seite 903-910 (DE-627)684567733 (DE-600)2648523-0 25889222 nnns volume:4 year:2014 number:11 pages:903-910 https://doi.org/10.12980/APJTB.4.201414B364 kostenfrei https://doaj.org/article/44eb13c2d74641edbc5bdc0f7324fcd3 kostenfrei http://www.sciencedirect.com/science/article/pii/S2221169115300381 kostenfrei https://doaj.org/toc/2221-1691 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 4 2014 11 903-910 |
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10.12980/APJTB.4.201414B364 doi (DE-627)DOAJ001388959 (DE-599)DOAJ44eb13c2d74641edbc5bdc0f7324fcd3 DE-627 ger DE-627 rakwb eng RC955-962 QH301-705.5 Emmanuel Anyachukwu Irondi verfasserin aut Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. Hyperglycemia Diabetic complications Enzyme inhibition Lipid peroxidation Mangifera indica Mucuna urens Arctic medicine. Tropical medicine Biology (General) Ganiyu Oboh verfasserin aut Afolabi Akintunde Akindahunsi verfasserin aut Aline Augusti Boligon verfasserin aut Margareth Linde Athayde verfasserin aut In Asian Pacific Journal of Tropical Biomedicine Wolters Kluwer Medknow Publications, 2016 4(2014), 11, Seite 903-910 (DE-627)684567733 (DE-600)2648523-0 25889222 nnns volume:4 year:2014 number:11 pages:903-910 https://doi.org/10.12980/APJTB.4.201414B364 kostenfrei https://doaj.org/article/44eb13c2d74641edbc5bdc0f7324fcd3 kostenfrei http://www.sciencedirect.com/science/article/pii/S2221169115300381 kostenfrei https://doaj.org/toc/2221-1691 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 4 2014 11 903-910 |
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10.12980/APJTB.4.201414B364 doi (DE-627)DOAJ001388959 (DE-599)DOAJ44eb13c2d74641edbc5bdc0f7324fcd3 DE-627 ger DE-627 rakwb eng RC955-962 QH301-705.5 Emmanuel Anyachukwu Irondi verfasserin aut Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. Hyperglycemia Diabetic complications Enzyme inhibition Lipid peroxidation Mangifera indica Mucuna urens Arctic medicine. Tropical medicine Biology (General) Ganiyu Oboh verfasserin aut Afolabi Akintunde Akindahunsi verfasserin aut Aline Augusti Boligon verfasserin aut Margareth Linde Athayde verfasserin aut In Asian Pacific Journal of Tropical Biomedicine Wolters Kluwer Medknow Publications, 2016 4(2014), 11, Seite 903-910 (DE-627)684567733 (DE-600)2648523-0 25889222 nnns volume:4 year:2014 number:11 pages:903-910 https://doi.org/10.12980/APJTB.4.201414B364 kostenfrei https://doaj.org/article/44eb13c2d74641edbc5bdc0f7324fcd3 kostenfrei http://www.sciencedirect.com/science/article/pii/S2221169115300381 kostenfrei https://doaj.org/toc/2221-1691 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 4 2014 11 903-910 |
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10.12980/APJTB.4.201414B364 doi (DE-627)DOAJ001388959 (DE-599)DOAJ44eb13c2d74641edbc5bdc0f7324fcd3 DE-627 ger DE-627 rakwb eng RC955-962 QH301-705.5 Emmanuel Anyachukwu Irondi verfasserin aut Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. Hyperglycemia Diabetic complications Enzyme inhibition Lipid peroxidation Mangifera indica Mucuna urens Arctic medicine. Tropical medicine Biology (General) Ganiyu Oboh verfasserin aut Afolabi Akintunde Akindahunsi verfasserin aut Aline Augusti Boligon verfasserin aut Margareth Linde Athayde verfasserin aut In Asian Pacific Journal of Tropical Biomedicine Wolters Kluwer Medknow Publications, 2016 4(2014), 11, Seite 903-910 (DE-627)684567733 (DE-600)2648523-0 25889222 nnns volume:4 year:2014 number:11 pages:903-910 https://doi.org/10.12980/APJTB.4.201414B364 kostenfrei https://doaj.org/article/44eb13c2d74641edbc5bdc0f7324fcd3 kostenfrei http://www.sciencedirect.com/science/article/pii/S2221169115300381 kostenfrei https://doaj.org/toc/2221-1691 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 4 2014 11 903-910 |
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10.12980/APJTB.4.201414B364 doi (DE-627)DOAJ001388959 (DE-599)DOAJ44eb13c2d74641edbc5bdc0f7324fcd3 DE-627 ger DE-627 rakwb eng RC955-962 QH301-705.5 Emmanuel Anyachukwu Irondi verfasserin aut Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. Hyperglycemia Diabetic complications Enzyme inhibition Lipid peroxidation Mangifera indica Mucuna urens Arctic medicine. Tropical medicine Biology (General) Ganiyu Oboh verfasserin aut Afolabi Akintunde Akindahunsi verfasserin aut Aline Augusti Boligon verfasserin aut Margareth Linde Athayde verfasserin aut In Asian Pacific Journal of Tropical Biomedicine Wolters Kluwer Medknow Publications, 2016 4(2014), 11, Seite 903-910 (DE-627)684567733 (DE-600)2648523-0 25889222 nnns volume:4 year:2014 number:11 pages:903-910 https://doi.org/10.12980/APJTB.4.201414B364 kostenfrei https://doaj.org/article/44eb13c2d74641edbc5bdc0f7324fcd3 kostenfrei http://www.sciencedirect.com/science/article/pii/S2221169115300381 kostenfrei https://doaj.org/toc/2221-1691 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 4 2014 11 903-910 |
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In Asian Pacific Journal of Tropical Biomedicine 4(2014), 11, Seite 903-910 volume:4 year:2014 number:11 pages:903-910 |
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In Asian Pacific Journal of Tropical Biomedicine 4(2014), 11, Seite 903-910 volume:4 year:2014 number:11 pages:903-910 |
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Hyperglycemia Diabetic complications Enzyme inhibition Lipid peroxidation Mangifera indica Mucuna urens Arctic medicine. Tropical medicine Biology (General) |
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Emmanuel Anyachukwu Irondi @@aut@@ Ganiyu Oboh @@aut@@ Afolabi Akintunde Akindahunsi @@aut@@ Aline Augusti Boligon @@aut@@ Margareth Linde Athayde @@aut@@ |
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Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. 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Emmanuel Anyachukwu Irondi |
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Emmanuel Anyachukwu Irondi misc RC955-962 misc QH301-705.5 misc Hyperglycemia misc Diabetic complications misc Enzyme inhibition misc Lipid peroxidation misc Mangifera indica misc Mucuna urens misc Arctic medicine. Tropical medicine misc Biology (General) Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes |
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RC955-962 QH301-705.5 Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes Hyperglycemia Diabetic complications Enzyme inhibition Lipid peroxidation Mangifera indica Mucuna urens |
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misc RC955-962 misc QH301-705.5 misc Hyperglycemia misc Diabetic complications misc Enzyme inhibition misc Lipid peroxidation misc Mangifera indica misc Mucuna urens misc Arctic medicine. Tropical medicine misc Biology (General) |
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Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes |
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Emmanuel Anyachukwu Irondi Ganiyu Oboh Afolabi Akintunde Akindahunsi Aline Augusti Boligon Margareth Linde Athayde |
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phenolic composition and inhibitory activity of mangifera indica and mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes |
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Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes |
| abstract |
Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. |
| abstractGer |
Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. |
| abstract_unstemmed |
Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase<α-glucosidase<α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. |
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| title_short |
Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes |
| url |
https://doi.org/10.12980/APJTB.4.201414B364 https://doaj.org/article/44eb13c2d74641edbc5bdc0f7324fcd3 http://www.sciencedirect.com/science/article/pii/S2221169115300381 https://doaj.org/toc/2221-1691 |
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| author2 |
Ganiyu Oboh Afolabi Akintunde Akindahunsi Aline Augusti Boligon Margareth Linde Athayde |
| author2Str |
Ganiyu Oboh Afolabi Akintunde Akindahunsi Aline Augusti Boligon Margareth Linde Athayde |
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684567733 |
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RC - Internal Medicine |
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| doi_str |
10.12980/APJTB.4.201414B364 |
| callnumber-a |
RC955-962 |
| up_date |
2024-07-03T20:08:30.380Z |
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| score |
7.399534 |