Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells

(1) Background: The aim of this study was to optimize, through a cheap and facile protocol, the covalent functionalization of graphene oxide (GO)-decorated cortical membrane (Lamina<sup<®</sup<) in order to promote the adhesion, the growth and the osteogenic differentiation of DPSCs (Den...
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Autor*in:	Roberta Di Carlo [verfasserIn] Susi Zara [verfasserIn] Alessia Ventrella [verfasserIn] Gabriella Siani [verfasserIn] Tatiana Da Ros [verfasserIn] Giovanna Iezzi [verfasserIn] Amelia Cataldi [verfasserIn] Antonella Fontana [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	graphene oxide covalent functionalization cortical membranes calcium phosphate deposition

Übergeordnetes Werk:	In: Nanomaterials - MDPI AG, 2012, 9(2019), 4, p 604
Übergeordnetes Werk:	volume:9 ; year:2019 ; number:4, p 604

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/nano9040604

Katalog-ID:	DOAJ00189241X

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spelling	10.3390/nano9040604 doi (DE-627)DOAJ00189241X (DE-599)DOAJ51dabaf89de74b8d9cff6eea99121ba2 DE-627 ger DE-627 rakwb eng QD1-999 Roberta Di Carlo verfasserin aut Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Background: The aim of this study was to optimize, through a cheap and facile protocol, the covalent functionalization of graphene oxide (GO)-decorated cortical membrane (Lamina<sup<®</sup<) in order to promote the adhesion, the growth and the osteogenic differentiation of DPSCs (Dental Pulp Stem Cells); (2) Methods: GO-coated Laminas were fully characterized by Scannsion Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analyses. In vitro analyses of viability, membrane integrity and calcium phosphate deposition were performed; (3) Results: The GO-decorated Laminas demonstrated an increase in the roughness of Laminas, a reduction in toxicity and did not affect membrane integrity of DPSCs; and (4) Conclusions: The GO covalent functionalization of Laminas was effective and relatively easy to obtain. The homogeneous GO coating obtained favored the proliferation rate of DPSCs and the deposition of calcium phosphate. graphene oxide covalent functionalization cortical membranes calcium phosphate deposition Chemistry Susi Zara verfasserin aut Alessia Ventrella verfasserin aut Gabriella Siani verfasserin aut Tatiana Da Ros verfasserin aut Giovanna Iezzi verfasserin aut Amelia Cataldi verfasserin aut Antonella Fontana verfasserin aut In Nanomaterials MDPI AG, 2012 9(2019), 4, p 604 (DE-627)718627199 (DE-600)2662255-5 20794991 nnns volume:9 year:2019 number:4, p 604 https://doi.org/10.3390/nano9040604 kostenfrei https://doaj.org/article/51dabaf89de74b8d9cff6eea99121ba2 kostenfrei https://www.mdpi.com/2079-4991/9/4/604 kostenfrei https://doaj.org/toc/2079-4991 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2019 4, p 604
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allfieldsGer	10.3390/nano9040604 doi (DE-627)DOAJ00189241X (DE-599)DOAJ51dabaf89de74b8d9cff6eea99121ba2 DE-627 ger DE-627 rakwb eng QD1-999 Roberta Di Carlo verfasserin aut Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Background: The aim of this study was to optimize, through a cheap and facile protocol, the covalent functionalization of graphene oxide (GO)-decorated cortical membrane (Lamina<sup<®</sup<) in order to promote the adhesion, the growth and the osteogenic differentiation of DPSCs (Dental Pulp Stem Cells); (2) Methods: GO-coated Laminas were fully characterized by Scannsion Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analyses. In vitro analyses of viability, membrane integrity and calcium phosphate deposition were performed; (3) Results: The GO-decorated Laminas demonstrated an increase in the roughness of Laminas, a reduction in toxicity and did not affect membrane integrity of DPSCs; and (4) Conclusions: The GO covalent functionalization of Laminas was effective and relatively easy to obtain. The homogeneous GO coating obtained favored the proliferation rate of DPSCs and the deposition of calcium phosphate. graphene oxide covalent functionalization cortical membranes calcium phosphate deposition Chemistry Susi Zara verfasserin aut Alessia Ventrella verfasserin aut Gabriella Siani verfasserin aut Tatiana Da Ros verfasserin aut Giovanna Iezzi verfasserin aut Amelia Cataldi verfasserin aut Antonella Fontana verfasserin aut In Nanomaterials MDPI AG, 2012 9(2019), 4, p 604 (DE-627)718627199 (DE-600)2662255-5 20794991 nnns volume:9 year:2019 number:4, p 604 https://doi.org/10.3390/nano9040604 kostenfrei https://doaj.org/article/51dabaf89de74b8d9cff6eea99121ba2 kostenfrei https://www.mdpi.com/2079-4991/9/4/604 kostenfrei https://doaj.org/toc/2079-4991 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2019 4, p 604
allfieldsSound	10.3390/nano9040604 doi (DE-627)DOAJ00189241X (DE-599)DOAJ51dabaf89de74b8d9cff6eea99121ba2 DE-627 ger DE-627 rakwb eng QD1-999 Roberta Di Carlo verfasserin aut Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Background: The aim of this study was to optimize, through a cheap and facile protocol, the covalent functionalization of graphene oxide (GO)-decorated cortical membrane (Lamina<sup<®</sup<) in order to promote the adhesion, the growth and the osteogenic differentiation of DPSCs (Dental Pulp Stem Cells); (2) Methods: GO-coated Laminas were fully characterized by Scannsion Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analyses. In vitro analyses of viability, membrane integrity and calcium phosphate deposition were performed; (3) Results: The GO-decorated Laminas demonstrated an increase in the roughness of Laminas, a reduction in toxicity and did not affect membrane integrity of DPSCs; and (4) Conclusions: The GO covalent functionalization of Laminas was effective and relatively easy to obtain. The homogeneous GO coating obtained favored the proliferation rate of DPSCs and the deposition of calcium phosphate. graphene oxide covalent functionalization cortical membranes calcium phosphate deposition Chemistry Susi Zara verfasserin aut Alessia Ventrella verfasserin aut Gabriella Siani verfasserin aut Tatiana Da Ros verfasserin aut Giovanna Iezzi verfasserin aut Amelia Cataldi verfasserin aut Antonella Fontana verfasserin aut In Nanomaterials MDPI AG, 2012 9(2019), 4, p 604 (DE-627)718627199 (DE-600)2662255-5 20794991 nnns volume:9 year:2019 number:4, p 604 https://doi.org/10.3390/nano9040604 kostenfrei https://doaj.org/article/51dabaf89de74b8d9cff6eea99121ba2 kostenfrei https://www.mdpi.com/2079-4991/9/4/604 kostenfrei https://doaj.org/toc/2079-4991 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2019 4, p 604
language	English
source	In Nanomaterials 9(2019), 4, p 604 volume:9 year:2019 number:4, p 604
sourceStr	In Nanomaterials 9(2019), 4, p 604 volume:9 year:2019 number:4, p 604
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	graphene oxide covalent functionalization cortical membranes calcium phosphate deposition Chemistry
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container_title	Nanomaterials
authorswithroles_txt_mv	Roberta Di Carlo @@aut@@ Susi Zara @@aut@@ Alessia Ventrella @@aut@@ Gabriella Siani @@aut@@ Tatiana Da Ros @@aut@@ Giovanna Iezzi @@aut@@ Amelia Cataldi @@aut@@ Antonella Fontana @@aut@@
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callnumber-first	Q - Science
author	Roberta Di Carlo
spellingShingle	Roberta Di Carlo misc QD1-999 misc graphene oxide misc covalent functionalization misc cortical membranes misc calcium phosphate deposition misc Chemistry Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells
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topic_title	QD1-999 Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells graphene oxide covalent functionalization cortical membranes calcium phosphate deposition
topic	misc QD1-999 misc graphene oxide misc covalent functionalization misc cortical membranes misc calcium phosphate deposition misc Chemistry
topic_unstemmed	misc QD1-999 misc graphene oxide misc covalent functionalization misc cortical membranes misc calcium phosphate deposition misc Chemistry
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title	Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells
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title_full	Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells
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author_browse	Roberta Di Carlo Susi Zara Alessia Ventrella Gabriella Siani Tatiana Da Ros Giovanna Iezzi Amelia Cataldi Antonella Fontana
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title_sort	covalent decoration of cortical membranes with graphene oxide as a substrate for dental pulp stem cells
callnumber	QD1-999
title_auth	Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells
abstract	(1) Background: The aim of this study was to optimize, through a cheap and facile protocol, the covalent functionalization of graphene oxide (GO)-decorated cortical membrane (Lamina<sup<®</sup<) in order to promote the adhesion, the growth and the osteogenic differentiation of DPSCs (Dental Pulp Stem Cells); (2) Methods: GO-coated Laminas were fully characterized by Scannsion Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analyses. In vitro analyses of viability, membrane integrity and calcium phosphate deposition were performed; (3) Results: The GO-decorated Laminas demonstrated an increase in the roughness of Laminas, a reduction in toxicity and did not affect membrane integrity of DPSCs; and (4) Conclusions: The GO covalent functionalization of Laminas was effective and relatively easy to obtain. The homogeneous GO coating obtained favored the proliferation rate of DPSCs and the deposition of calcium phosphate.
abstractGer	(1) Background: The aim of this study was to optimize, through a cheap and facile protocol, the covalent functionalization of graphene oxide (GO)-decorated cortical membrane (Lamina<sup<®</sup<) in order to promote the adhesion, the growth and the osteogenic differentiation of DPSCs (Dental Pulp Stem Cells); (2) Methods: GO-coated Laminas were fully characterized by Scannsion Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analyses. In vitro analyses of viability, membrane integrity and calcium phosphate deposition were performed; (3) Results: The GO-decorated Laminas demonstrated an increase in the roughness of Laminas, a reduction in toxicity and did not affect membrane integrity of DPSCs; and (4) Conclusions: The GO covalent functionalization of Laminas was effective and relatively easy to obtain. The homogeneous GO coating obtained favored the proliferation rate of DPSCs and the deposition of calcium phosphate.
abstract_unstemmed	(1) Background: The aim of this study was to optimize, through a cheap and facile protocol, the covalent functionalization of graphene oxide (GO)-decorated cortical membrane (Lamina<sup<®</sup<) in order to promote the adhesion, the growth and the osteogenic differentiation of DPSCs (Dental Pulp Stem Cells); (2) Methods: GO-coated Laminas were fully characterized by Scannsion Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analyses. In vitro analyses of viability, membrane integrity and calcium phosphate deposition were performed; (3) Results: The GO-decorated Laminas demonstrated an increase in the roughness of Laminas, a reduction in toxicity and did not affect membrane integrity of DPSCs; and (4) Conclusions: The GO covalent functionalization of Laminas was effective and relatively easy to obtain. The homogeneous GO coating obtained favored the proliferation rate of DPSCs and the deposition of calcium phosphate.
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container_issue	4, p 604
title_short	Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells
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