Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage

BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular leve...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Linjian Mo [verfasserIn] Zhenyuan Yu [verfasserIn] Yufang Lv [verfasserIn] Jiwen Cheng [verfasserIn] Haibiao Yan [verfasserIn] Wenhao Lu [verfasserIn] Cheng Su [verfasserIn] Qiang Ling [verfasserIn] Zengnan Mo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	testicular seminoma single-cell RNA sequencing scRNA-seq lymph node metastasis tumor-immune microenvironment

Übergeordnetes Werk:	In: Frontiers in Oncology - Frontiers Media S.A., 2012, 12(2022)
Übergeordnetes Werk:	volume:12 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fonc.2022.871489

Katalog-ID:	DOAJ002348136

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520			\|a BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma.
650		4	\|a testicular seminoma
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650		4	\|a tumor-immune microenvironment
653		0	\|a Neoplasms. Tumors. Oncology. Including cancer and carcinogens
700	0		\|a Linjian Mo \|e verfasserin \|4 aut
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allfields	10.3389/fonc.2022.871489 doi (DE-627)DOAJ002348136 (DE-599)DOAJe21a4e982bc948f3bd040a133b686c88 DE-627 ger DE-627 rakwb eng RC254-282 Linjian Mo verfasserin aut Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma. testicular seminoma single-cell RNA sequencing scRNA-seq lymph node metastasis tumor-immune microenvironment Neoplasms. Tumors. Oncology. Including cancer and carcinogens Linjian Mo verfasserin aut Zhenyuan Yu verfasserin aut Zhenyuan Yu verfasserin aut Yufang Lv verfasserin aut Yufang Lv verfasserin aut Jiwen Cheng verfasserin aut Haibiao Yan verfasserin aut Wenhao Lu verfasserin aut Wenhao Lu verfasserin aut Cheng Su verfasserin aut Cheng Su verfasserin aut Qiang Ling verfasserin aut Qiang Ling verfasserin aut Zengnan Mo verfasserin aut Zengnan Mo verfasserin aut In Frontiers in Oncology Frontiers Media S.A., 2012 12(2022) (DE-627)684965518 (DE-600)2649216-7 2234943X nnns volume:12 year:2022 https://doi.org/10.3389/fonc.2022.871489 kostenfrei https://doaj.org/article/e21a4e982bc948f3bd040a133b686c88 kostenfrei https://www.frontiersin.org/articles/10.3389/fonc.2022.871489/full kostenfrei https://doaj.org/toc/2234-943X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
spelling	10.3389/fonc.2022.871489 doi (DE-627)DOAJ002348136 (DE-599)DOAJe21a4e982bc948f3bd040a133b686c88 DE-627 ger DE-627 rakwb eng RC254-282 Linjian Mo verfasserin aut Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma. testicular seminoma single-cell RNA sequencing scRNA-seq lymph node metastasis tumor-immune microenvironment Neoplasms. Tumors. Oncology. Including cancer and carcinogens Linjian Mo verfasserin aut Zhenyuan Yu verfasserin aut Zhenyuan Yu verfasserin aut Yufang Lv verfasserin aut Yufang Lv verfasserin aut Jiwen Cheng verfasserin aut Haibiao Yan verfasserin aut Wenhao Lu verfasserin aut Wenhao Lu verfasserin aut Cheng Su verfasserin aut Cheng Su verfasserin aut Qiang Ling verfasserin aut Qiang Ling verfasserin aut Zengnan Mo verfasserin aut Zengnan Mo verfasserin aut In Frontiers in Oncology Frontiers Media S.A., 2012 12(2022) (DE-627)684965518 (DE-600)2649216-7 2234943X nnns volume:12 year:2022 https://doi.org/10.3389/fonc.2022.871489 kostenfrei https://doaj.org/article/e21a4e982bc948f3bd040a133b686c88 kostenfrei https://www.frontiersin.org/articles/10.3389/fonc.2022.871489/full kostenfrei https://doaj.org/toc/2234-943X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
allfields_unstemmed	10.3389/fonc.2022.871489 doi (DE-627)DOAJ002348136 (DE-599)DOAJe21a4e982bc948f3bd040a133b686c88 DE-627 ger DE-627 rakwb eng RC254-282 Linjian Mo verfasserin aut Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma. testicular seminoma single-cell RNA sequencing scRNA-seq lymph node metastasis tumor-immune microenvironment Neoplasms. Tumors. Oncology. Including cancer and carcinogens Linjian Mo verfasserin aut Zhenyuan Yu verfasserin aut Zhenyuan Yu verfasserin aut Yufang Lv verfasserin aut Yufang Lv verfasserin aut Jiwen Cheng verfasserin aut Haibiao Yan verfasserin aut Wenhao Lu verfasserin aut Wenhao Lu verfasserin aut Cheng Su verfasserin aut Cheng Su verfasserin aut Qiang Ling verfasserin aut Qiang Ling verfasserin aut Zengnan Mo verfasserin aut Zengnan Mo verfasserin aut In Frontiers in Oncology Frontiers Media S.A., 2012 12(2022) (DE-627)684965518 (DE-600)2649216-7 2234943X nnns volume:12 year:2022 https://doi.org/10.3389/fonc.2022.871489 kostenfrei https://doaj.org/article/e21a4e982bc948f3bd040a133b686c88 kostenfrei https://www.frontiersin.org/articles/10.3389/fonc.2022.871489/full kostenfrei https://doaj.org/toc/2234-943X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
allfieldsGer	10.3389/fonc.2022.871489 doi (DE-627)DOAJ002348136 (DE-599)DOAJe21a4e982bc948f3bd040a133b686c88 DE-627 ger DE-627 rakwb eng RC254-282 Linjian Mo verfasserin aut Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma. testicular seminoma single-cell RNA sequencing scRNA-seq lymph node metastasis tumor-immune microenvironment Neoplasms. Tumors. Oncology. Including cancer and carcinogens Linjian Mo verfasserin aut Zhenyuan Yu verfasserin aut Zhenyuan Yu verfasserin aut Yufang Lv verfasserin aut Yufang Lv verfasserin aut Jiwen Cheng verfasserin aut Haibiao Yan verfasserin aut Wenhao Lu verfasserin aut Wenhao Lu verfasserin aut Cheng Su verfasserin aut Cheng Su verfasserin aut Qiang Ling verfasserin aut Qiang Ling verfasserin aut Zengnan Mo verfasserin aut Zengnan Mo verfasserin aut In Frontiers in Oncology Frontiers Media S.A., 2012 12(2022) (DE-627)684965518 (DE-600)2649216-7 2234943X nnns volume:12 year:2022 https://doi.org/10.3389/fonc.2022.871489 kostenfrei https://doaj.org/article/e21a4e982bc948f3bd040a133b686c88 kostenfrei https://www.frontiersin.org/articles/10.3389/fonc.2022.871489/full kostenfrei https://doaj.org/toc/2234-943X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
allfieldsSound	10.3389/fonc.2022.871489 doi (DE-627)DOAJ002348136 (DE-599)DOAJe21a4e982bc948f3bd040a133b686c88 DE-627 ger DE-627 rakwb eng RC254-282 Linjian Mo verfasserin aut Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma. testicular seminoma single-cell RNA sequencing scRNA-seq lymph node metastasis tumor-immune microenvironment Neoplasms. Tumors. Oncology. Including cancer and carcinogens Linjian Mo verfasserin aut Zhenyuan Yu verfasserin aut Zhenyuan Yu verfasserin aut Yufang Lv verfasserin aut Yufang Lv verfasserin aut Jiwen Cheng verfasserin aut Haibiao Yan verfasserin aut Wenhao Lu verfasserin aut Wenhao Lu verfasserin aut Cheng Su verfasserin aut Cheng Su verfasserin aut Qiang Ling verfasserin aut Qiang Ling verfasserin aut Zengnan Mo verfasserin aut Zengnan Mo verfasserin aut In Frontiers in Oncology Frontiers Media S.A., 2012 12(2022) (DE-627)684965518 (DE-600)2649216-7 2234943X nnns volume:12 year:2022 https://doi.org/10.3389/fonc.2022.871489 kostenfrei https://doaj.org/article/e21a4e982bc948f3bd040a133b686c88 kostenfrei https://www.frontiersin.org/articles/10.3389/fonc.2022.871489/full kostenfrei https://doaj.org/toc/2234-943X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
language	English
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topic_facet	testicular seminoma single-cell RNA sequencing scRNA-seq lymph node metastasis tumor-immune microenvironment Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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authorswithroles_txt_mv	Linjian Mo @@aut@@ Zhenyuan Yu @@aut@@ Yufang Lv @@aut@@ Jiwen Cheng @@aut@@ Haibiao Yan @@aut@@ Wenhao Lu @@aut@@ Cheng Su @@aut@@ Qiang Ling @@aut@@ Zengnan Mo @@aut@@
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callnumber-first	R - Medicine
author	Linjian Mo
spellingShingle	Linjian Mo misc RC254-282 misc testicular seminoma misc single-cell RNA sequencing misc scRNA-seq misc lymph node metastasis misc tumor-immune microenvironment misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage
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topic_title	RC254-282 Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage testicular seminoma single-cell RNA sequencing scRNA-seq lymph node metastasis tumor-immune microenvironment
topic	misc RC254-282 misc testicular seminoma misc single-cell RNA sequencing misc scRNA-seq misc lymph node metastasis misc tumor-immune microenvironment misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc testicular seminoma misc single-cell RNA sequencing misc scRNA-seq misc lymph node metastasis misc tumor-immune microenvironment misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_browse	misc RC254-282 misc testicular seminoma misc single-cell RNA sequencing misc scRNA-seq misc lymph node metastasis misc tumor-immune microenvironment misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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title	Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage
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title_full	Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage
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title_sort	single-cell rna sequencing of metastatic testicular seminoma reveals the cellular and molecular characteristics of metastatic cell lineage
callnumber	RC254-282
title_auth	Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage
abstract	BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma.
abstractGer	BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma.
abstract_unstemmed	BackgroundTesticular cancer is the most common solid malignancy in young men. Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma.
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title_short	Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage
url	https://doi.org/10.3389/fonc.2022.871489 https://doaj.org/article/e21a4e982bc948f3bd040a133b686c88 https://www.frontiersin.org/articles/10.3389/fonc.2022.871489/full https://doaj.org/toc/2234-943X
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author2	Linjian Mo Zhenyuan Yu Yufang Lv Jiwen Cheng Haibiao Yan Wenhao Lu Cheng Su Qiang Ling Zengnan Mo
author2Str	Linjian Mo Zhenyuan Yu Yufang Lv Jiwen Cheng Haibiao Yan Wenhao Lu Cheng Su Qiang Ling Zengnan Mo
ppnlink	684965518
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doi_str	10.3389/fonc.2022.871489
callnumber-a	RC254-282
up_date	2024-07-04T00:51:57.250Z
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Given the many histological classifications of testicular tumors, seminoma is one of the most treatable cancers. The survival rate in early-stage disease was more than 90%. Thus, seminoma at the cellular and molecular levels, especially at the single-cell level, is worth studying.MethodsWe performed a single-cell RNA sequencing (scRNA-seq) study on a patient who was diagnosed with testicular seminoma with lymph node metastasis. This study presented tumor tissue, PBMC, pelvic and renal hilus lymph node in a total of 18,206 high-quality single-cell transcriptome information. The characteristics of metastatic cell lineage were revealed by the comparison between different tumor cell subtypes at the scRNA level.ResultsA single-cell map of testicular seminoma with lymph node metastasis was constructed by scRNA-seq. We discovered the gene expression characteristics of the tumor cells in testicular seminoma, especially metastatic tumor cells. KRT8 and KRT18 were commonly expressed in the three tumor cell subtypes. However, TCF7L1, SCG3 and SV2C were the specifically expressed genes of tumor cell subtypes in primary tumor sites. Some molecular markers specifically expressed by the metastatic cell lineage, such as POU5F1, were identified.ConclusionsWe revealed the molecular characteristics of testicular seminoma at the single-cell level, especially the metastatic tumor cells. This study could provide new insights into the diagnosis and treatment of testicular seminoma.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">testicular seminoma</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">single-cell RNA sequencing</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">scRNA-seq</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">lymph node metastasis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">tumor-immune microenvironment</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Neoplasms. Tumors. Oncology. 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Single-Cell RNA Sequencing of Metastatic Testicular Seminoma Reveals the Cellular and Molecular Characteristics of Metastatic Cell Lineage

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