Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing

Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another huma...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Madeleine Heep [verfasserIn] Pia Mach [verfasserIn] Philipp Reautschnig [verfasserIn] Jacqueline Wettengel [verfasserIn] Thorsten Stafforst [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	site-directed RNA editing ADAR guideRNA genetic disease RNA repair

Übergeordnetes Werk:	In: Genes - MDPI AG, 2010, 8(2017), 1, p 34
Übergeordnetes Werk:	volume:8 ; year:2017 ; number:1, p 34

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/genes8010034

Katalog-ID:	DOAJ002433540

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spelling	10.3390/genes8010034 doi (DE-627)DOAJ002433540 (DE-599)DOAJ5c2aa00822a646a6ab90aaa98af7d78d DE-627 ger DE-627 rakwb eng QH426-470 Madeleine Heep verfasserin aut Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing. site-directed RNA editing ADAR guideRNA genetic disease RNA repair Genetics Pia Mach verfasserin aut Philipp Reautschnig verfasserin aut Jacqueline Wettengel verfasserin aut Thorsten Stafforst verfasserin aut In Genes MDPI AG, 2010 8(2017), 1, p 34 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:8 year:2017 number:1, p 34 https://doi.org/10.3390/genes8010034 kostenfrei https://doaj.org/article/5c2aa00822a646a6ab90aaa98af7d78d kostenfrei http://www.mdpi.com/2073-4425/8/1/34 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2017 1, p 34
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allfieldsGer	10.3390/genes8010034 doi (DE-627)DOAJ002433540 (DE-599)DOAJ5c2aa00822a646a6ab90aaa98af7d78d DE-627 ger DE-627 rakwb eng QH426-470 Madeleine Heep verfasserin aut Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing. site-directed RNA editing ADAR guideRNA genetic disease RNA repair Genetics Pia Mach verfasserin aut Philipp Reautschnig verfasserin aut Jacqueline Wettengel verfasserin aut Thorsten Stafforst verfasserin aut In Genes MDPI AG, 2010 8(2017), 1, p 34 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:8 year:2017 number:1, p 34 https://doi.org/10.3390/genes8010034 kostenfrei https://doaj.org/article/5c2aa00822a646a6ab90aaa98af7d78d kostenfrei http://www.mdpi.com/2073-4425/8/1/34 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2017 1, p 34
allfieldsSound	10.3390/genes8010034 doi (DE-627)DOAJ002433540 (DE-599)DOAJ5c2aa00822a646a6ab90aaa98af7d78d DE-627 ger DE-627 rakwb eng QH426-470 Madeleine Heep verfasserin aut Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing. site-directed RNA editing ADAR guideRNA genetic disease RNA repair Genetics Pia Mach verfasserin aut Philipp Reautschnig verfasserin aut Jacqueline Wettengel verfasserin aut Thorsten Stafforst verfasserin aut In Genes MDPI AG, 2010 8(2017), 1, p 34 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:8 year:2017 number:1, p 34 https://doi.org/10.3390/genes8010034 kostenfrei https://doaj.org/article/5c2aa00822a646a6ab90aaa98af7d78d kostenfrei http://www.mdpi.com/2073-4425/8/1/34 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2017 1, p 34
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callnumber-first	Q - Science
author	Madeleine Heep
spellingShingle	Madeleine Heep misc QH426-470 misc site-directed RNA editing misc ADAR misc guideRNA misc genetic disease misc RNA repair misc Genetics Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing
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topic_title	QH426-470 Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing site-directed RNA editing ADAR guideRNA genetic disease RNA repair
topic	misc QH426-470 misc site-directed RNA editing misc ADAR misc guideRNA misc genetic disease misc RNA repair misc Genetics
topic_unstemmed	misc QH426-470 misc site-directed RNA editing misc ADAR misc guideRNA misc genetic disease misc RNA repair misc Genetics
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title	Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing
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title_full	Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing
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title_sort	applying human adar1p110 and adar1p150 for site-directed rna editing—g/c substitution stabilizes guidernas against editing
callnumber	QH426-470
title_auth	Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing
abstract	Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing.
abstractGer	Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing.
abstract_unstemmed	Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing.
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container_issue	1, p 34
title_short	Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing
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Vorhandene Bände

Nicht das Richtige dabei?