Spectrophotometric Determination of Thiamine hydrochloride Via Oxidative Coupling Reaction Using 4-Aminoantipyrine
A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The p...
Ausführliche Beschreibung
Autor*in: |
Reem Al-Luhaiby [verfasserIn] Mohammed Alenizzi [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Arabisch ; Englisch |
Erschienen: |
2020 |
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Übergeordnetes Werk: |
In: مجلة التربية والعلم - College of Education for Pure Sciences, 2019, 29(2020), 4, Seite 62-75 |
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Übergeordnetes Werk: |
volume:29 ; year:2020 ; number:4 ; pages:62-75 |
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DOI / URN: |
10.33899/edusj.2020.126895.1062 |
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Katalog-ID: |
DOAJ003135284 |
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520 | |a A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The product show maximum absorption at 335 nm. The molar absorptivitiy is 26410.59 l/mol.cm for concentrations obeyed Beer’s law in the range 0.2-18 μg.ml-1. The recovery was 100.93 % with relative standard deviation < 2.0 % for thiamine hydrochloride.Thiamine and reagent 4-AAP product was formed in the ratio of 1:1. The stability constant of the product was 3.39×106 l.mol-1 for thiamine hydrochloride indicating the good stability of this product. The optimum conditions for full colour development are described and the proposed method was applied successfully for determination of thiamine in the pharmaceutical preparation (Neurorubine). The common excipients used as additives in pharmaceutical do not interfere in the proposed method. | ||
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10.33899/edusj.2020.126895.1062 doi (DE-627)DOAJ003135284 (DE-599)DOAJ52f91151753247489bed50436fcd4d72 DE-627 ger DE-627 rakwb ara eng Q1-390 Reem Al-Luhaiby verfasserin aut Spectrophotometric Determination of Thiamine hydrochloride Via Oxidative Coupling Reaction Using 4-Aminoantipyrine 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The product show maximum absorption at 335 nm. The molar absorptivitiy is 26410.59 l/mol.cm for concentrations obeyed Beer’s law in the range 0.2-18 μg.ml-1. The recovery was 100.93 % with relative standard deviation < 2.0 % for thiamine hydrochloride.Thiamine and reagent 4-AAP product was formed in the ratio of 1:1. The stability constant of the product was 3.39×106 l.mol-1 for thiamine hydrochloride indicating the good stability of this product. The optimum conditions for full colour development are described and the proposed method was applied successfully for determination of thiamine in the pharmaceutical preparation (Neurorubine). The common excipients used as additives in pharmaceutical do not interfere in the proposed method. oxidative coupling,, ,،,؛spectrophotometry,, ,،,؛thiamine hydrochloride,, ,،,؛4-aminoantipyrine Education L Science (General) Mohammed Alenizzi verfasserin aut In مجلة التربية والعلم College of Education for Pure Sciences, 2019 29(2020), 4, Seite 62-75 (DE-627)734147023 (DE-600)2697544-0 26642530 nnns volume:29 year:2020 number:4 pages:62-75 https://doi.org/10.33899/edusj.2020.126895.1062 kostenfrei https://doaj.org/article/52f91151753247489bed50436fcd4d72 kostenfrei https://edusj.mosuljournals.com/article_165390_ae4ce8dc4d57b82cb88567cb1372d755.pdf kostenfrei https://doaj.org/toc/1812-125X Journal toc kostenfrei https://doaj.org/toc/2664-2530 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 29 2020 4 62-75 |
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10.33899/edusj.2020.126895.1062 doi (DE-627)DOAJ003135284 (DE-599)DOAJ52f91151753247489bed50436fcd4d72 DE-627 ger DE-627 rakwb ara eng Q1-390 Reem Al-Luhaiby verfasserin aut Spectrophotometric Determination of Thiamine hydrochloride Via Oxidative Coupling Reaction Using 4-Aminoantipyrine 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The product show maximum absorption at 335 nm. The molar absorptivitiy is 26410.59 l/mol.cm for concentrations obeyed Beer’s law in the range 0.2-18 μg.ml-1. The recovery was 100.93 % with relative standard deviation < 2.0 % for thiamine hydrochloride.Thiamine and reagent 4-AAP product was formed in the ratio of 1:1. The stability constant of the product was 3.39×106 l.mol-1 for thiamine hydrochloride indicating the good stability of this product. The optimum conditions for full colour development are described and the proposed method was applied successfully for determination of thiamine in the pharmaceutical preparation (Neurorubine). The common excipients used as additives in pharmaceutical do not interfere in the proposed method. oxidative coupling,, ,،,؛spectrophotometry,, ,،,؛thiamine hydrochloride,, ,،,؛4-aminoantipyrine Education L Science (General) Mohammed Alenizzi verfasserin aut In مجلة التربية والعلم College of Education for Pure Sciences, 2019 29(2020), 4, Seite 62-75 (DE-627)734147023 (DE-600)2697544-0 26642530 nnns volume:29 year:2020 number:4 pages:62-75 https://doi.org/10.33899/edusj.2020.126895.1062 kostenfrei https://doaj.org/article/52f91151753247489bed50436fcd4d72 kostenfrei https://edusj.mosuljournals.com/article_165390_ae4ce8dc4d57b82cb88567cb1372d755.pdf kostenfrei https://doaj.org/toc/1812-125X Journal toc kostenfrei https://doaj.org/toc/2664-2530 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 29 2020 4 62-75 |
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10.33899/edusj.2020.126895.1062 doi (DE-627)DOAJ003135284 (DE-599)DOAJ52f91151753247489bed50436fcd4d72 DE-627 ger DE-627 rakwb ara eng Q1-390 Reem Al-Luhaiby verfasserin aut Spectrophotometric Determination of Thiamine hydrochloride Via Oxidative Coupling Reaction Using 4-Aminoantipyrine 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The product show maximum absorption at 335 nm. The molar absorptivitiy is 26410.59 l/mol.cm for concentrations obeyed Beer’s law in the range 0.2-18 μg.ml-1. The recovery was 100.93 % with relative standard deviation < 2.0 % for thiamine hydrochloride.Thiamine and reagent 4-AAP product was formed in the ratio of 1:1. The stability constant of the product was 3.39×106 l.mol-1 for thiamine hydrochloride indicating the good stability of this product. The optimum conditions for full colour development are described and the proposed method was applied successfully for determination of thiamine in the pharmaceutical preparation (Neurorubine). The common excipients used as additives in pharmaceutical do not interfere in the proposed method. oxidative coupling,, ,،,؛spectrophotometry,, ,،,؛thiamine hydrochloride,, ,،,؛4-aminoantipyrine Education L Science (General) Mohammed Alenizzi verfasserin aut In مجلة التربية والعلم College of Education for Pure Sciences, 2019 29(2020), 4, Seite 62-75 (DE-627)734147023 (DE-600)2697544-0 26642530 nnns volume:29 year:2020 number:4 pages:62-75 https://doi.org/10.33899/edusj.2020.126895.1062 kostenfrei https://doaj.org/article/52f91151753247489bed50436fcd4d72 kostenfrei https://edusj.mosuljournals.com/article_165390_ae4ce8dc4d57b82cb88567cb1372d755.pdf kostenfrei https://doaj.org/toc/1812-125X Journal toc kostenfrei https://doaj.org/toc/2664-2530 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 29 2020 4 62-75 |
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10.33899/edusj.2020.126895.1062 doi (DE-627)DOAJ003135284 (DE-599)DOAJ52f91151753247489bed50436fcd4d72 DE-627 ger DE-627 rakwb ara eng Q1-390 Reem Al-Luhaiby verfasserin aut Spectrophotometric Determination of Thiamine hydrochloride Via Oxidative Coupling Reaction Using 4-Aminoantipyrine 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The product show maximum absorption at 335 nm. The molar absorptivitiy is 26410.59 l/mol.cm for concentrations obeyed Beer’s law in the range 0.2-18 μg.ml-1. The recovery was 100.93 % with relative standard deviation < 2.0 % for thiamine hydrochloride.Thiamine and reagent 4-AAP product was formed in the ratio of 1:1. The stability constant of the product was 3.39×106 l.mol-1 for thiamine hydrochloride indicating the good stability of this product. The optimum conditions for full colour development are described and the proposed method was applied successfully for determination of thiamine in the pharmaceutical preparation (Neurorubine). The common excipients used as additives in pharmaceutical do not interfere in the proposed method. oxidative coupling,, ,،,؛spectrophotometry,, ,،,؛thiamine hydrochloride,, ,،,؛4-aminoantipyrine Education L Science (General) Mohammed Alenizzi verfasserin aut In مجلة التربية والعلم College of Education for Pure Sciences, 2019 29(2020), 4, Seite 62-75 (DE-627)734147023 (DE-600)2697544-0 26642530 nnns volume:29 year:2020 number:4 pages:62-75 https://doi.org/10.33899/edusj.2020.126895.1062 kostenfrei https://doaj.org/article/52f91151753247489bed50436fcd4d72 kostenfrei https://edusj.mosuljournals.com/article_165390_ae4ce8dc4d57b82cb88567cb1372d755.pdf kostenfrei https://doaj.org/toc/1812-125X Journal toc kostenfrei https://doaj.org/toc/2664-2530 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 29 2020 4 62-75 |
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Q1-390 Spectrophotometric Determination of Thiamine hydrochloride Via Oxidative Coupling Reaction Using 4-Aminoantipyrine oxidative coupling ,،,؛spectrophotometry ,،,؛thiamine hydrochloride ,،,؛4-aminoantipyrine |
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Spectrophotometric Determination of Thiamine hydrochloride Via Oxidative Coupling Reaction Using 4-Aminoantipyrine |
abstract |
A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The product show maximum absorption at 335 nm. The molar absorptivitiy is 26410.59 l/mol.cm for concentrations obeyed Beer’s law in the range 0.2-18 μg.ml-1. The recovery was 100.93 % with relative standard deviation < 2.0 % for thiamine hydrochloride.Thiamine and reagent 4-AAP product was formed in the ratio of 1:1. The stability constant of the product was 3.39×106 l.mol-1 for thiamine hydrochloride indicating the good stability of this product. The optimum conditions for full colour development are described and the proposed method was applied successfully for determination of thiamine in the pharmaceutical preparation (Neurorubine). The common excipients used as additives in pharmaceutical do not interfere in the proposed method. |
abstractGer |
A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The product show maximum absorption at 335 nm. The molar absorptivitiy is 26410.59 l/mol.cm for concentrations obeyed Beer’s law in the range 0.2-18 μg.ml-1. The recovery was 100.93 % with relative standard deviation < 2.0 % for thiamine hydrochloride.Thiamine and reagent 4-AAP product was formed in the ratio of 1:1. The stability constant of the product was 3.39×106 l.mol-1 for thiamine hydrochloride indicating the good stability of this product. The optimum conditions for full colour development are described and the proposed method was applied successfully for determination of thiamine in the pharmaceutical preparation (Neurorubine). The common excipients used as additives in pharmaceutical do not interfere in the proposed method. |
abstract_unstemmed |
A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The product show maximum absorption at 335 nm. The molar absorptivitiy is 26410.59 l/mol.cm for concentrations obeyed Beer’s law in the range 0.2-18 μg.ml-1. The recovery was 100.93 % with relative standard deviation < 2.0 % for thiamine hydrochloride.Thiamine and reagent 4-AAP product was formed in the ratio of 1:1. The stability constant of the product was 3.39×106 l.mol-1 for thiamine hydrochloride indicating the good stability of this product. The optimum conditions for full colour development are described and the proposed method was applied successfully for determination of thiamine in the pharmaceutical preparation (Neurorubine). The common excipients used as additives in pharmaceutical do not interfere in the proposed method. |
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Spectrophotometric Determination of Thiamine hydrochloride Via Oxidative Coupling Reaction Using 4-Aminoantipyrine |
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