Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells

Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe ste...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Keltouma Benmeziane [verfasserIn] Benoit Delache [verfasserIn] Sébastien Langlois [verfasserIn] Gabriella Scarlatti [verfasserIn] Roger Le Grand [verfasserIn] Mariangela Cavarelli [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Cell isolation Flow Cytometry/Mass Cytometry Immunology

Übergeordnetes Werk:	In: STAR Protocols - Elsevier, 2020, 3(2022), 4, Seite 101815-
Übergeordnetes Werk:	volume:3 ; year:2022 ; number:4 ; pages:101815-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.xpro.2022.101815

Katalog-ID:	DOAJ003339831

Internformat


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publishDate	2022
allfields	10.1016/j.xpro.2022.101815 doi (DE-627)DOAJ003339831 (DE-599)DOAJ0b959893e0e3404586001793c5c6a871 DE-627 ger DE-627 rakwb eng Q1-390 Keltouma Benmeziane verfasserin aut Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications.For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell isolation Flow Cytometry/Mass Cytometry Immunology Science (General) Benoit Delache verfasserin aut Sébastien Langlois verfasserin aut Gabriella Scarlatti verfasserin aut Roger Le Grand verfasserin aut Mariangela Cavarelli verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 4, Seite 101815- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:4 pages:101815- https://doi.org/10.1016/j.xpro.2022.101815 kostenfrei https://doaj.org/article/0b959893e0e3404586001793c5c6a871 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722006955 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 4 101815-
spelling	10.1016/j.xpro.2022.101815 doi (DE-627)DOAJ003339831 (DE-599)DOAJ0b959893e0e3404586001793c5c6a871 DE-627 ger DE-627 rakwb eng Q1-390 Keltouma Benmeziane verfasserin aut Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications.For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell isolation Flow Cytometry/Mass Cytometry Immunology Science (General) Benoit Delache verfasserin aut Sébastien Langlois verfasserin aut Gabriella Scarlatti verfasserin aut Roger Le Grand verfasserin aut Mariangela Cavarelli verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 4, Seite 101815- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:4 pages:101815- https://doi.org/10.1016/j.xpro.2022.101815 kostenfrei https://doaj.org/article/0b959893e0e3404586001793c5c6a871 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722006955 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 4 101815-
allfields_unstemmed	10.1016/j.xpro.2022.101815 doi (DE-627)DOAJ003339831 (DE-599)DOAJ0b959893e0e3404586001793c5c6a871 DE-627 ger DE-627 rakwb eng Q1-390 Keltouma Benmeziane verfasserin aut Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications.For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell isolation Flow Cytometry/Mass Cytometry Immunology Science (General) Benoit Delache verfasserin aut Sébastien Langlois verfasserin aut Gabriella Scarlatti verfasserin aut Roger Le Grand verfasserin aut Mariangela Cavarelli verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 4, Seite 101815- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:4 pages:101815- https://doi.org/10.1016/j.xpro.2022.101815 kostenfrei https://doaj.org/article/0b959893e0e3404586001793c5c6a871 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722006955 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 4 101815-
allfieldsGer	10.1016/j.xpro.2022.101815 doi (DE-627)DOAJ003339831 (DE-599)DOAJ0b959893e0e3404586001793c5c6a871 DE-627 ger DE-627 rakwb eng Q1-390 Keltouma Benmeziane verfasserin aut Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications.For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell isolation Flow Cytometry/Mass Cytometry Immunology Science (General) Benoit Delache verfasserin aut Sébastien Langlois verfasserin aut Gabriella Scarlatti verfasserin aut Roger Le Grand verfasserin aut Mariangela Cavarelli verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 4, Seite 101815- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:4 pages:101815- https://doi.org/10.1016/j.xpro.2022.101815 kostenfrei https://doaj.org/article/0b959893e0e3404586001793c5c6a871 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722006955 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 4 101815-
allfieldsSound	10.1016/j.xpro.2022.101815 doi (DE-627)DOAJ003339831 (DE-599)DOAJ0b959893e0e3404586001793c5c6a871 DE-627 ger DE-627 rakwb eng Q1-390 Keltouma Benmeziane verfasserin aut Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications.For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell isolation Flow Cytometry/Mass Cytometry Immunology Science (General) Benoit Delache verfasserin aut Sébastien Langlois verfasserin aut Gabriella Scarlatti verfasserin aut Roger Le Grand verfasserin aut Mariangela Cavarelli verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 4, Seite 101815- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:4 pages:101815- https://doi.org/10.1016/j.xpro.2022.101815 kostenfrei https://doaj.org/article/0b959893e0e3404586001793c5c6a871 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722006955 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 4 101815-
language	English
source	In STAR Protocols 3(2022), 4, Seite 101815- volume:3 year:2022 number:4 pages:101815-
sourceStr	In STAR Protocols 3(2022), 4, Seite 101815- volume:3 year:2022 number:4 pages:101815-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Cell isolation Flow Cytometry/Mass Cytometry Immunology Science (General)
isfreeaccess_bool	true
container_title	STAR Protocols
authorswithroles_txt_mv	Keltouma Benmeziane @@aut@@ Benoit Delache @@aut@@ Sébastien Langlois @@aut@@ Gabriella Scarlatti @@aut@@ Roger Le Grand @@aut@@ Mariangela Cavarelli @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	1747970557
id	DOAJ003339831
language_de	englisch
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callnumber-first	Q - Science
author	Keltouma Benmeziane
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topic_title	Q1-390 Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells Cell isolation Flow Cytometry/Mass Cytometry Immunology
topic	misc Q1-390 misc Cell isolation misc Flow Cytometry/Mass Cytometry misc Immunology misc Science (General)
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title	Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells
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title_full	Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells
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author_browse	Keltouma Benmeziane Benoit Delache Sébastien Langlois Gabriella Scarlatti Roger Le Grand Mariangela Cavarelli
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title_sort	isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells
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title_auth	Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells
abstract	Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications.For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
abstractGer	Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications.For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
abstract_unstemmed	Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications.For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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title_short	Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells
url	https://doi.org/10.1016/j.xpro.2022.101815 https://doaj.org/article/0b959893e0e3404586001793c5c6a871 http://www.sciencedirect.com/science/article/pii/S2666166722006955 https://doaj.org/toc/2666-1667
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up_date	2024-07-03T17:25:40.922Z
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Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells

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