Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex

Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system....
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Autor*in:	Colin Echeverría Aitken [verfasserIn] Petra Beznosková [verfasserIn] Vladislava Vlčkova [verfasserIn] Wen-Ling Chiu [verfasserIn] Fujun Zhou [verfasserIn] Leoš Shivaya Valášek [verfasserIn] Alan G Hinnebusch [verfasserIn] Jon R Lorsch [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	eIF3 translation initiation ribosome yeast mRNA recruitment

Übergeordnetes Werk:	In: eLife - eLife Sciences Publications Ltd, 2013, 5(2016)
Übergeordnetes Werk:	volume:5 ; year:2016

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.7554/eLife.20934

Katalog-ID:	DOAJ003353230

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allfields	10.7554/eLife.20934 doi (DE-627)DOAJ003353230 (DE-599)DOAJ5ae5eda69c8b4f079cbbfa66f1665ba9 DE-627 ger DE-627 rakwb eng QH301-705.5 Colin Echeverría Aitken verfasserin aut Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system. Mutations throughout eIF3 disrupt its interaction with the PIC and diminish its ability to accelerate recruitment to a native yeast mRNA. Alterations to the eIF3a CTD and eIF3b/i/g significantly slow mRNA recruitment, and mutations within eIF3b/i/g destabilize eIF2•GTP•Met-tRNAi binding to the PIC. Using model mRNAs lacking contacts with the 40S entry or exit channels, we uncovered a critical role for eIF3 requiring the eIF3a NTD, in stabilizing mRNA interactions at the exit channel, and an ancillary role at the entry channel requiring residues of the eIF3a CTD. These functions are redundant: defects at each channel can be rescued by filling the other channel with mRNA. eIF3 translation initiation ribosome yeast mRNA recruitment Medicine R Science Q Biology (General) Petra Beznosková verfasserin aut Vladislava Vlčkova verfasserin aut Wen-Ling Chiu verfasserin aut Fujun Zhou verfasserin aut Leoš Shivaya Valášek verfasserin aut Alan G Hinnebusch verfasserin aut Jon R Lorsch verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 5(2016) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:5 year:2016 https://doi.org/10.7554/eLife.20934 kostenfrei https://doaj.org/article/5ae5eda69c8b4f079cbbfa66f1665ba9 kostenfrei https://elifesciences.org/articles/20934 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016
spelling	10.7554/eLife.20934 doi (DE-627)DOAJ003353230 (DE-599)DOAJ5ae5eda69c8b4f079cbbfa66f1665ba9 DE-627 ger DE-627 rakwb eng QH301-705.5 Colin Echeverría Aitken verfasserin aut Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system. Mutations throughout eIF3 disrupt its interaction with the PIC and diminish its ability to accelerate recruitment to a native yeast mRNA. Alterations to the eIF3a CTD and eIF3b/i/g significantly slow mRNA recruitment, and mutations within eIF3b/i/g destabilize eIF2•GTP•Met-tRNAi binding to the PIC. Using model mRNAs lacking contacts with the 40S entry or exit channels, we uncovered a critical role for eIF3 requiring the eIF3a NTD, in stabilizing mRNA interactions at the exit channel, and an ancillary role at the entry channel requiring residues of the eIF3a CTD. These functions are redundant: defects at each channel can be rescued by filling the other channel with mRNA. eIF3 translation initiation ribosome yeast mRNA recruitment Medicine R Science Q Biology (General) Petra Beznosková verfasserin aut Vladislava Vlčkova verfasserin aut Wen-Ling Chiu verfasserin aut Fujun Zhou verfasserin aut Leoš Shivaya Valášek verfasserin aut Alan G Hinnebusch verfasserin aut Jon R Lorsch verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 5(2016) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:5 year:2016 https://doi.org/10.7554/eLife.20934 kostenfrei https://doaj.org/article/5ae5eda69c8b4f079cbbfa66f1665ba9 kostenfrei https://elifesciences.org/articles/20934 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016
allfields_unstemmed	10.7554/eLife.20934 doi (DE-627)DOAJ003353230 (DE-599)DOAJ5ae5eda69c8b4f079cbbfa66f1665ba9 DE-627 ger DE-627 rakwb eng QH301-705.5 Colin Echeverría Aitken verfasserin aut Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system. Mutations throughout eIF3 disrupt its interaction with the PIC and diminish its ability to accelerate recruitment to a native yeast mRNA. Alterations to the eIF3a CTD and eIF3b/i/g significantly slow mRNA recruitment, and mutations within eIF3b/i/g destabilize eIF2•GTP•Met-tRNAi binding to the PIC. Using model mRNAs lacking contacts with the 40S entry or exit channels, we uncovered a critical role for eIF3 requiring the eIF3a NTD, in stabilizing mRNA interactions at the exit channel, and an ancillary role at the entry channel requiring residues of the eIF3a CTD. These functions are redundant: defects at each channel can be rescued by filling the other channel with mRNA. eIF3 translation initiation ribosome yeast mRNA recruitment Medicine R Science Q Biology (General) Petra Beznosková verfasserin aut Vladislava Vlčkova verfasserin aut Wen-Ling Chiu verfasserin aut Fujun Zhou verfasserin aut Leoš Shivaya Valášek verfasserin aut Alan G Hinnebusch verfasserin aut Jon R Lorsch verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 5(2016) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:5 year:2016 https://doi.org/10.7554/eLife.20934 kostenfrei https://doaj.org/article/5ae5eda69c8b4f079cbbfa66f1665ba9 kostenfrei https://elifesciences.org/articles/20934 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016
allfieldsGer	10.7554/eLife.20934 doi (DE-627)DOAJ003353230 (DE-599)DOAJ5ae5eda69c8b4f079cbbfa66f1665ba9 DE-627 ger DE-627 rakwb eng QH301-705.5 Colin Echeverría Aitken verfasserin aut Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system. Mutations throughout eIF3 disrupt its interaction with the PIC and diminish its ability to accelerate recruitment to a native yeast mRNA. Alterations to the eIF3a CTD and eIF3b/i/g significantly slow mRNA recruitment, and mutations within eIF3b/i/g destabilize eIF2•GTP•Met-tRNAi binding to the PIC. Using model mRNAs lacking contacts with the 40S entry or exit channels, we uncovered a critical role for eIF3 requiring the eIF3a NTD, in stabilizing mRNA interactions at the exit channel, and an ancillary role at the entry channel requiring residues of the eIF3a CTD. These functions are redundant: defects at each channel can be rescued by filling the other channel with mRNA. eIF3 translation initiation ribosome yeast mRNA recruitment Medicine R Science Q Biology (General) Petra Beznosková verfasserin aut Vladislava Vlčkova verfasserin aut Wen-Ling Chiu verfasserin aut Fujun Zhou verfasserin aut Leoš Shivaya Valášek verfasserin aut Alan G Hinnebusch verfasserin aut Jon R Lorsch verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 5(2016) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:5 year:2016 https://doi.org/10.7554/eLife.20934 kostenfrei https://doaj.org/article/5ae5eda69c8b4f079cbbfa66f1665ba9 kostenfrei https://elifesciences.org/articles/20934 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016
allfieldsSound	10.7554/eLife.20934 doi (DE-627)DOAJ003353230 (DE-599)DOAJ5ae5eda69c8b4f079cbbfa66f1665ba9 DE-627 ger DE-627 rakwb eng QH301-705.5 Colin Echeverría Aitken verfasserin aut Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system. Mutations throughout eIF3 disrupt its interaction with the PIC and diminish its ability to accelerate recruitment to a native yeast mRNA. Alterations to the eIF3a CTD and eIF3b/i/g significantly slow mRNA recruitment, and mutations within eIF3b/i/g destabilize eIF2•GTP•Met-tRNAi binding to the PIC. Using model mRNAs lacking contacts with the 40S entry or exit channels, we uncovered a critical role for eIF3 requiring the eIF3a NTD, in stabilizing mRNA interactions at the exit channel, and an ancillary role at the entry channel requiring residues of the eIF3a CTD. These functions are redundant: defects at each channel can be rescued by filling the other channel with mRNA. eIF3 translation initiation ribosome yeast mRNA recruitment Medicine R Science Q Biology (General) Petra Beznosková verfasserin aut Vladislava Vlčkova verfasserin aut Wen-Ling Chiu verfasserin aut Fujun Zhou verfasserin aut Leoš Shivaya Valášek verfasserin aut Alan G Hinnebusch verfasserin aut Jon R Lorsch verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 5(2016) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:5 year:2016 https://doi.org/10.7554/eLife.20934 kostenfrei https://doaj.org/article/5ae5eda69c8b4f079cbbfa66f1665ba9 kostenfrei https://elifesciences.org/articles/20934 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016
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author	Colin Echeverría Aitken
spellingShingle	Colin Echeverría Aitken misc QH301-705.5 misc eIF3 misc translation misc initiation misc ribosome misc yeast misc mRNA recruitment misc Medicine misc R misc Science misc Q misc Biology (General) Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex
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topic_title	QH301-705.5 Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex eIF3 translation initiation ribosome yeast mRNA recruitment
topic	misc QH301-705.5 misc eIF3 misc translation misc initiation misc ribosome misc yeast misc mRNA recruitment misc Medicine misc R misc Science misc Q misc Biology (General)
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title	Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex
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title_full	Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex
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title_sort	eukaryotic translation initiation factor 3 plays distinct roles at the mrna entry and exit channels of the ribosomal preinitiation complex
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title_auth	Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex
abstract	Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system. Mutations throughout eIF3 disrupt its interaction with the PIC and diminish its ability to accelerate recruitment to a native yeast mRNA. Alterations to the eIF3a CTD and eIF3b/i/g significantly slow mRNA recruitment, and mutations within eIF3b/i/g destabilize eIF2•GTP•Met-tRNAi binding to the PIC. Using model mRNAs lacking contacts with the 40S entry or exit channels, we uncovered a critical role for eIF3 requiring the eIF3a NTD, in stabilizing mRNA interactions at the exit channel, and an ancillary role at the entry channel requiring residues of the eIF3a CTD. These functions are redundant: defects at each channel can be rescued by filling the other channel with mRNA.
abstractGer	Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system. Mutations throughout eIF3 disrupt its interaction with the PIC and diminish its ability to accelerate recruitment to a native yeast mRNA. Alterations to the eIF3a CTD and eIF3b/i/g significantly slow mRNA recruitment, and mutations within eIF3b/i/g destabilize eIF2•GTP•Met-tRNAi binding to the PIC. Using model mRNAs lacking contacts with the 40S entry or exit channels, we uncovered a critical role for eIF3 requiring the eIF3a NTD, in stabilizing mRNA interactions at the exit channel, and an ancillary role at the entry channel requiring residues of the eIF3a CTD. These functions are redundant: defects at each channel can be rescued by filling the other channel with mRNA.
abstract_unstemmed	Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system. Mutations throughout eIF3 disrupt its interaction with the PIC and diminish its ability to accelerate recruitment to a native yeast mRNA. Alterations to the eIF3a CTD and eIF3b/i/g significantly slow mRNA recruitment, and mutations within eIF3b/i/g destabilize eIF2•GTP•Met-tRNAi binding to the PIC. Using model mRNAs lacking contacts with the 40S entry or exit channels, we uncovered a critical role for eIF3 requiring the eIF3a NTD, in stabilizing mRNA interactions at the exit channel, and an ancillary role at the entry channel requiring residues of the eIF3a CTD. These functions are redundant: defects at each channel can be rescued by filling the other channel with mRNA.
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title_short	Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex
url	https://doi.org/10.7554/eLife.20934 https://doaj.org/article/5ae5eda69c8b4f079cbbfa66f1665ba9 https://elifesciences.org/articles/20934 https://doaj.org/toc/2050-084X
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Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex

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