Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I
Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid S...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Babakhanzadeh E [verfasserIn] Khodadadian A [verfasserIn] Nazari M [verfasserIn] Dehghan Tezerjani M [verfasserIn] Aghaei SM [verfasserIn] Ghasemifar S [verfasserIn] Hosseinnia M [verfasserIn] Mazaheri M [verfasserIn] |
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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In: International Journal of General Medicine - Dove Medical Press, 2008, (2020), Seite 185-192 |
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| Übergeordnetes Werk: |
year:2020 ; pages:185-192 |
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DOAJ003388182 |
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| 520 | |a Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran; 4Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran*These authors contributed equally to this workCorrespondence: Mahta Mazaheri Email mahta.mazaheri2019gmail.comIntroduction: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis.Methods: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA- in 34 patients.Results: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA- group in comparison to NOA+ group (p< 0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples.Discussion: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.Keywords: DGCR8, obstructive azoospermia, non-obstructive azoospermia, spermatogenesis | ||
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(DE-627)DOAJ003388182 (DE-599)DOAJ4f5373d8238c4360866b82c7abd06153 DE-627 ger DE-627 rakwb eng R5-920 Babakhanzadeh E verfasserin aut Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran; 4Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran*These authors contributed equally to this workCorrespondence: Mahta Mazaheri Email mahta.mazaheri2019gmail.comIntroduction: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis.Methods: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA- in 34 patients.Results: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA- group in comparison to NOA+ group (p< 0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples.Discussion: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.Keywords: DGCR8, obstructive azoospermia, non-obstructive azoospermia, spermatogenesis dgcr8 obstructive azoospermia non-obstructive azoospermia spermatogenesis Medicine (General) Khodadadian A verfasserin aut Nazari M verfasserin aut Dehghan Tezerjani M verfasserin aut Aghaei SM verfasserin aut Ghasemifar S verfasserin aut Hosseinnia M verfasserin aut Mazaheri M verfasserin aut In International Journal of General Medicine Dove Medical Press, 2008 (2020), Seite 185-192 (DE-627)578539691 (DE-600)2452220-X 11787074 nnns year:2020 pages:185-192 https://doaj.org/article/4f5373d8238c4360866b82c7abd06153 kostenfrei https://www.dovepress.com/deficient-expression-of-dgcr8-in-human-testis-is-related-to-spermatoge-peer-reviewed-article-IJGM kostenfrei https://doaj.org/toc/1178-7074 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 185-192 |
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(DE-627)DOAJ003388182 (DE-599)DOAJ4f5373d8238c4360866b82c7abd06153 DE-627 ger DE-627 rakwb eng R5-920 Babakhanzadeh E verfasserin aut Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran; 4Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran*These authors contributed equally to this workCorrespondence: Mahta Mazaheri Email mahta.mazaheri2019gmail.comIntroduction: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis.Methods: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA- in 34 patients.Results: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA- group in comparison to NOA+ group (p< 0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples.Discussion: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.Keywords: DGCR8, obstructive azoospermia, non-obstructive azoospermia, spermatogenesis dgcr8 obstructive azoospermia non-obstructive azoospermia spermatogenesis Medicine (General) Khodadadian A verfasserin aut Nazari M verfasserin aut Dehghan Tezerjani M verfasserin aut Aghaei SM verfasserin aut Ghasemifar S verfasserin aut Hosseinnia M verfasserin aut Mazaheri M verfasserin aut In International Journal of General Medicine Dove Medical Press, 2008 (2020), Seite 185-192 (DE-627)578539691 (DE-600)2452220-X 11787074 nnns year:2020 pages:185-192 https://doaj.org/article/4f5373d8238c4360866b82c7abd06153 kostenfrei https://www.dovepress.com/deficient-expression-of-dgcr8-in-human-testis-is-related-to-spermatoge-peer-reviewed-article-IJGM kostenfrei https://doaj.org/toc/1178-7074 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 185-192 |
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(DE-627)DOAJ003388182 (DE-599)DOAJ4f5373d8238c4360866b82c7abd06153 DE-627 ger DE-627 rakwb eng R5-920 Babakhanzadeh E verfasserin aut Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran; 4Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran*These authors contributed equally to this workCorrespondence: Mahta Mazaheri Email mahta.mazaheri2019gmail.comIntroduction: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis.Methods: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA- in 34 patients.Results: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA- group in comparison to NOA+ group (p< 0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples.Discussion: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.Keywords: DGCR8, obstructive azoospermia, non-obstructive azoospermia, spermatogenesis dgcr8 obstructive azoospermia non-obstructive azoospermia spermatogenesis Medicine (General) Khodadadian A verfasserin aut Nazari M verfasserin aut Dehghan Tezerjani M verfasserin aut Aghaei SM verfasserin aut Ghasemifar S verfasserin aut Hosseinnia M verfasserin aut Mazaheri M verfasserin aut In International Journal of General Medicine Dove Medical Press, 2008 (2020), Seite 185-192 (DE-627)578539691 (DE-600)2452220-X 11787074 nnns year:2020 pages:185-192 https://doaj.org/article/4f5373d8238c4360866b82c7abd06153 kostenfrei https://www.dovepress.com/deficient-expression-of-dgcr8-in-human-testis-is-related-to-spermatoge-peer-reviewed-article-IJGM kostenfrei https://doaj.org/toc/1178-7074 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 185-192 |
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(DE-627)DOAJ003388182 (DE-599)DOAJ4f5373d8238c4360866b82c7abd06153 DE-627 ger DE-627 rakwb eng R5-920 Babakhanzadeh E verfasserin aut Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran; 4Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran*These authors contributed equally to this workCorrespondence: Mahta Mazaheri Email mahta.mazaheri2019gmail.comIntroduction: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis.Methods: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA- in 34 patients.Results: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA- group in comparison to NOA+ group (p< 0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples.Discussion: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.Keywords: DGCR8, obstructive azoospermia, non-obstructive azoospermia, spermatogenesis dgcr8 obstructive azoospermia non-obstructive azoospermia spermatogenesis Medicine (General) Khodadadian A verfasserin aut Nazari M verfasserin aut Dehghan Tezerjani M verfasserin aut Aghaei SM verfasserin aut Ghasemifar S verfasserin aut Hosseinnia M verfasserin aut Mazaheri M verfasserin aut In International Journal of General Medicine Dove Medical Press, 2008 (2020), Seite 185-192 (DE-627)578539691 (DE-600)2452220-X 11787074 nnns year:2020 pages:185-192 https://doaj.org/article/4f5373d8238c4360866b82c7abd06153 kostenfrei https://www.dovepress.com/deficient-expression-of-dgcr8-in-human-testis-is-related-to-spermatoge-peer-reviewed-article-IJGM kostenfrei https://doaj.org/toc/1178-7074 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 185-192 |
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(DE-627)DOAJ003388182 (DE-599)DOAJ4f5373d8238c4360866b82c7abd06153 DE-627 ger DE-627 rakwb eng R5-920 Babakhanzadeh E verfasserin aut Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran; 4Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran*These authors contributed equally to this workCorrespondence: Mahta Mazaheri Email mahta.mazaheri2019gmail.comIntroduction: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis.Methods: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA- in 34 patients.Results: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA- group in comparison to NOA+ group (p< 0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples.Discussion: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.Keywords: DGCR8, obstructive azoospermia, non-obstructive azoospermia, spermatogenesis dgcr8 obstructive azoospermia non-obstructive azoospermia spermatogenesis Medicine (General) Khodadadian A verfasserin aut Nazari M verfasserin aut Dehghan Tezerjani M verfasserin aut Aghaei SM verfasserin aut Ghasemifar S verfasserin aut Hosseinnia M verfasserin aut Mazaheri M verfasserin aut In International Journal of General Medicine Dove Medical Press, 2008 (2020), Seite 185-192 (DE-627)578539691 (DE-600)2452220-X 11787074 nnns year:2020 pages:185-192 https://doaj.org/article/4f5373d8238c4360866b82c7abd06153 kostenfrei https://www.dovepress.com/deficient-expression-of-dgcr8-in-human-testis-is-related-to-spermatoge-peer-reviewed-article-IJGM kostenfrei https://doaj.org/toc/1178-7074 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 185-192 |
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Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I |
| abstract |
Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran; 4Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran*These authors contributed equally to this workCorrespondence: Mahta Mazaheri Email mahta.mazaheri2019gmail.comIntroduction: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis.Methods: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA- in 34 patients.Results: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA- group in comparison to NOA+ group (p< 0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples.Discussion: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.Keywords: DGCR8, obstructive azoospermia, non-obstructive azoospermia, spermatogenesis |
| abstractGer |
Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran; 4Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran*These authors contributed equally to this workCorrespondence: Mahta Mazaheri Email mahta.mazaheri2019gmail.comIntroduction: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis.Methods: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA- in 34 patients.Results: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA- group in comparison to NOA+ group (p< 0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples.Discussion: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.Keywords: DGCR8, obstructive azoospermia, non-obstructive azoospermia, spermatogenesis |
| abstract_unstemmed |
Emad Babakhanzadeh,1,2,* Ali Khodadadian,1,* Majid Nazari,1 Masoud Dehghan Tezerjani,1 Seyed Mohsen Aghaei,1 Sina Ghasemifar,1 Mehdi Hosseinnia,3 Mahta Mazaheri1,4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran; 4Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran*These authors contributed equally to this workCorrespondence: Mahta Mazaheri Email mahta.mazaheri2019gmail.comIntroduction: DiGeorge syndrome critical region gene 8 (DGCR8) contributes to miRNA biogenesis, and defects in its expression could lead to defects in spermatogenesis.Methods: Here, we assess gene and protein expression levels of DGCR8 in the testicular biopsy specimens obtained from men with obstructive azoospermia (OA, n = 19) and various types of non-obstructive azoospermia (NOA) including maturation arrest (MA, n = 17), Sertoli cell-only syndrome (SCOS, n = 20) and hypospermatogenesis (HYPO, 18). Also, samples of men with NOA were divided into two groups based on successful and unsuccessful sperm recovery, NOA+ in 21 patients and NOA- in 34 patients.Results: Examinations disclosed a severe decrease in DGCR8 in samples with MA and SCOS in comparison to OA samples (P < 0.001). Also, the results showed DGCR8 has significantly lower expression in testis tissues of NOA- group in comparison to NOA+ group (p< 0.05). Western blot analysis confirmed that the DGCR8 protein was not expressed in SCOS samples and had a very low expression in MA and HYPO samples.Discussion: The results of this survey showed that DGCR8 is an important gene for the entire spermatogenesis pathway. Moreover, DGCR8 gene plays an important role in the diagnosis of NOA subgroups, and also the expression changes in it might contribute to SCOS or MA phenotypes. This gene with considering other related genes can also be a predictor of sperm retrieval.Keywords: DGCR8, obstructive azoospermia, non-obstructive azoospermia, spermatogenesis |
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Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I |
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https://doaj.org/article/4f5373d8238c4360866b82c7abd06153 https://www.dovepress.com/deficient-expression-of-dgcr8-in-human-testis-is-related-to-spermatoge-peer-reviewed-article-IJGM https://doaj.org/toc/1178-7074 |
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