One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study

<i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection as...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Andrea Zendrini [verfasserIn] Valentina Carta [verfasserIn] Virginia Filipello [verfasserIn] Laura Ragni [verfasserIn] Elena Cosciani-Cunico [verfasserIn] Sara Arnaboldi [verfasserIn] Barbara Bertasi [verfasserIn] Niccolò Franceschi [verfasserIn] Paolo Ajmone-Marsan [verfasserIn] Dario De Medici [verfasserIn] Marina Nadia Losio [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	LAMP poultry foodborne diseases

Übergeordnetes Werk:	In: Foods - MDPI AG, 2013, 10(2021), 5, p 1132
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:5, p 1132

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/foods10051132

Katalog-ID:	DOAJ00343947X

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ00343947X
003	DE-627
005	20240412180855.0
007	cr uuu---uuuuu
008	230225s2021 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.3390/foods10051132 \|2 doi
035			\|a (DE-627)DOAJ00343947X
035			\|a (DE-599)DOAJ510672a7632441f8866fd651eb869c6f
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a TP1-1185
100	0		\|a Andrea Zendrini \|e verfasserin \|4 aut
245	1	0	\|a One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study
264		1	\|c 2021
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a <i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation.
650		4	\|a LAMP
650		4	\|a <i<Campylobacter</i<
650		4	\|a <i<Salmonella</i<
650		4	\|a poultry
650		4	\|a foodborne diseases
653		0	\|a Chemical technology
700	0		\|a Valentina Carta \|e verfasserin \|4 aut
700	0		\|a Virginia Filipello \|e verfasserin \|4 aut
700	0		\|a Laura Ragni \|e verfasserin \|4 aut
700	0		\|a Elena Cosciani-Cunico \|e verfasserin \|4 aut
700	0		\|a Sara Arnaboldi \|e verfasserin \|4 aut
700	0		\|a Barbara Bertasi \|e verfasserin \|4 aut
700	0		\|a Niccolò Franceschi \|e verfasserin \|4 aut
700	0		\|a Paolo Ajmone-Marsan \|e verfasserin \|4 aut
700	0		\|a Dario De Medici \|e verfasserin \|4 aut
700	0		\|a Marina Nadia Losio \|e verfasserin \|4 aut
773	0	8	\|i In \|t Foods \|d MDPI AG, 2013 \|g 10(2021), 5, p 1132 \|w (DE-627)737287632 \|w (DE-600)2704223-6 \|x 23048158 \|7 nnns
773	1	8	\|g volume:10 \|g year:2021 \|g number:5, p 1132
856	4	0	\|u https://doi.org/10.3390/foods10051132 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/510672a7632441f8866fd651eb869c6f \|z kostenfrei
856	4	0	\|u https://www.mdpi.com/2304-8158/10/5/1132 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/2304-8158 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 10 \|j 2021 \|e 5, p 1132

Indexfelder

author_variant	a z az v c vc v f vf l r lr e c c ecc s a sa b b bb n f nf p a m pam d d m ddm m n l mnl
matchkey_str	article:23048158:2021----::ndyoeuadtcinfsloeliniaplbceinh
hierarchy_sort_str	2021
callnumber-subject-code	TP
publishDate	2021
allfields	10.3390/foods10051132 doi (DE-627)DOAJ00343947X (DE-599)DOAJ510672a7632441f8866fd651eb869c6f DE-627 ger DE-627 rakwb eng TP1-1185 Andrea Zendrini verfasserin aut One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation. LAMP <i<Campylobacter</i< <i<Salmonella</i< poultry foodborne diseases Chemical technology Valentina Carta verfasserin aut Virginia Filipello verfasserin aut Laura Ragni verfasserin aut Elena Cosciani-Cunico verfasserin aut Sara Arnaboldi verfasserin aut Barbara Bertasi verfasserin aut Niccolò Franceschi verfasserin aut Paolo Ajmone-Marsan verfasserin aut Dario De Medici verfasserin aut Marina Nadia Losio verfasserin aut In Foods MDPI AG, 2013 10(2021), 5, p 1132 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:10 year:2021 number:5, p 1132 https://doi.org/10.3390/foods10051132 kostenfrei https://doaj.org/article/510672a7632441f8866fd651eb869c6f kostenfrei https://www.mdpi.com/2304-8158/10/5/1132 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 5, p 1132
spelling	10.3390/foods10051132 doi (DE-627)DOAJ00343947X (DE-599)DOAJ510672a7632441f8866fd651eb869c6f DE-627 ger DE-627 rakwb eng TP1-1185 Andrea Zendrini verfasserin aut One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation. LAMP <i<Campylobacter</i< <i<Salmonella</i< poultry foodborne diseases Chemical technology Valentina Carta verfasserin aut Virginia Filipello verfasserin aut Laura Ragni verfasserin aut Elena Cosciani-Cunico verfasserin aut Sara Arnaboldi verfasserin aut Barbara Bertasi verfasserin aut Niccolò Franceschi verfasserin aut Paolo Ajmone-Marsan verfasserin aut Dario De Medici verfasserin aut Marina Nadia Losio verfasserin aut In Foods MDPI AG, 2013 10(2021), 5, p 1132 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:10 year:2021 number:5, p 1132 https://doi.org/10.3390/foods10051132 kostenfrei https://doaj.org/article/510672a7632441f8866fd651eb869c6f kostenfrei https://www.mdpi.com/2304-8158/10/5/1132 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 5, p 1132
allfields_unstemmed	10.3390/foods10051132 doi (DE-627)DOAJ00343947X (DE-599)DOAJ510672a7632441f8866fd651eb869c6f DE-627 ger DE-627 rakwb eng TP1-1185 Andrea Zendrini verfasserin aut One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation. LAMP <i<Campylobacter</i< <i<Salmonella</i< poultry foodborne diseases Chemical technology Valentina Carta verfasserin aut Virginia Filipello verfasserin aut Laura Ragni verfasserin aut Elena Cosciani-Cunico verfasserin aut Sara Arnaboldi verfasserin aut Barbara Bertasi verfasserin aut Niccolò Franceschi verfasserin aut Paolo Ajmone-Marsan verfasserin aut Dario De Medici verfasserin aut Marina Nadia Losio verfasserin aut In Foods MDPI AG, 2013 10(2021), 5, p 1132 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:10 year:2021 number:5, p 1132 https://doi.org/10.3390/foods10051132 kostenfrei https://doaj.org/article/510672a7632441f8866fd651eb869c6f kostenfrei https://www.mdpi.com/2304-8158/10/5/1132 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 5, p 1132
allfieldsGer	10.3390/foods10051132 doi (DE-627)DOAJ00343947X (DE-599)DOAJ510672a7632441f8866fd651eb869c6f DE-627 ger DE-627 rakwb eng TP1-1185 Andrea Zendrini verfasserin aut One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation. LAMP <i<Campylobacter</i< <i<Salmonella</i< poultry foodborne diseases Chemical technology Valentina Carta verfasserin aut Virginia Filipello verfasserin aut Laura Ragni verfasserin aut Elena Cosciani-Cunico verfasserin aut Sara Arnaboldi verfasserin aut Barbara Bertasi verfasserin aut Niccolò Franceschi verfasserin aut Paolo Ajmone-Marsan verfasserin aut Dario De Medici verfasserin aut Marina Nadia Losio verfasserin aut In Foods MDPI AG, 2013 10(2021), 5, p 1132 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:10 year:2021 number:5, p 1132 https://doi.org/10.3390/foods10051132 kostenfrei https://doaj.org/article/510672a7632441f8866fd651eb869c6f kostenfrei https://www.mdpi.com/2304-8158/10/5/1132 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 5, p 1132
allfieldsSound	10.3390/foods10051132 doi (DE-627)DOAJ00343947X (DE-599)DOAJ510672a7632441f8866fd651eb869c6f DE-627 ger DE-627 rakwb eng TP1-1185 Andrea Zendrini verfasserin aut One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation. LAMP <i<Campylobacter</i< <i<Salmonella</i< poultry foodborne diseases Chemical technology Valentina Carta verfasserin aut Virginia Filipello verfasserin aut Laura Ragni verfasserin aut Elena Cosciani-Cunico verfasserin aut Sara Arnaboldi verfasserin aut Barbara Bertasi verfasserin aut Niccolò Franceschi verfasserin aut Paolo Ajmone-Marsan verfasserin aut Dario De Medici verfasserin aut Marina Nadia Losio verfasserin aut In Foods MDPI AG, 2013 10(2021), 5, p 1132 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:10 year:2021 number:5, p 1132 https://doi.org/10.3390/foods10051132 kostenfrei https://doaj.org/article/510672a7632441f8866fd651eb869c6f kostenfrei https://www.mdpi.com/2304-8158/10/5/1132 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 5, p 1132
language	English
source	In Foods 10(2021), 5, p 1132 volume:10 year:2021 number:5, p 1132
sourceStr	In Foods 10(2021), 5, p 1132 volume:10 year:2021 number:5, p 1132
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	LAMP <i<Campylobacter</i< <i<Salmonella</i< poultry foodborne diseases Chemical technology
isfreeaccess_bool	true
container_title	Foods
authorswithroles_txt_mv	Andrea Zendrini @@aut@@ Valentina Carta @@aut@@ Virginia Filipello @@aut@@ Laura Ragni @@aut@@ Elena Cosciani-Cunico @@aut@@ Sara Arnaboldi @@aut@@ Barbara Bertasi @@aut@@ Niccolò Franceschi @@aut@@ Paolo Ajmone-Marsan @@aut@@ Dario De Medici @@aut@@ Marina Nadia Losio @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	737287632
id	DOAJ00343947X
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ00343947X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412180855.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230225s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/foods10051132</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ00343947X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ510672a7632441f8866fd651eb869c6f</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">TP1-1185</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Andrea Zendrini</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">LAMP</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Campylobacter</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Salmonella</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">poultry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">foodborne diseases</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Chemical technology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Valentina Carta</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Virginia Filipello</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Laura Ragni</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Elena Cosciani-Cunico</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sara Arnaboldi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Barbara Bertasi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Niccolò Franceschi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Paolo Ajmone-Marsan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Dario De Medici</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Marina Nadia Losio</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Foods</subfield><subfield code="d">MDPI AG, 2013</subfield><subfield code="g">10(2021), 5, p 1132</subfield><subfield code="w">(DE-627)737287632</subfield><subfield code="w">(DE-600)2704223-6</subfield><subfield code="x">23048158</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:10</subfield><subfield code="g">year:2021</subfield><subfield code="g">number:5, p 1132</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/foods10051132</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/510672a7632441f8866fd651eb869c6f</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/2304-8158/10/5/1132</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2304-8158</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">10</subfield><subfield code="j">2021</subfield><subfield code="e">5, p 1132</subfield></datafield></record></collection>
callnumber-first	T - Technology
author	Andrea Zendrini
spellingShingle	Andrea Zendrini misc TP1-1185 misc LAMP misc <i<Campylobacter</i< misc <i<Salmonella</i< misc poultry misc foodborne diseases misc Chemical technology One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study
authorStr	Andrea Zendrini
ppnlink_with_tag_str_mv	@@773@@(DE-627)737287632
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	TP1-1185
illustrated	Not Illustrated
issn	23048158
topic_title	TP1-1185 One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study LAMP <i<Campylobacter</i< <i<Salmonella</i< poultry foodborne diseases
topic	misc TP1-1185 misc LAMP misc <i<Campylobacter</i< misc <i<Salmonella</i< misc poultry misc foodborne diseases misc Chemical technology
topic_unstemmed	misc TP1-1185 misc LAMP misc <i<Campylobacter</i< misc <i<Salmonella</i< misc poultry misc foodborne diseases misc Chemical technology
topic_browse	misc TP1-1185 misc LAMP misc <i<Campylobacter</i< misc <i<Salmonella</i< misc poultry misc foodborne diseases misc Chemical technology
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Foods
hierarchy_parent_id	737287632
hierarchy_top_title	Foods
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)737287632 (DE-600)2704223-6
title	One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study
ctrlnum	(DE-627)DOAJ00343947X (DE-599)DOAJ510672a7632441f8866fd651eb869c6f
title_full	One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study
author_sort	Andrea Zendrini
journal	Foods
journalStr	Foods
callnumber-first-code	T
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2021
contenttype_str_mv	txt
author_browse	Andrea Zendrini Valentina Carta Virginia Filipello Laura Ragni Elena Cosciani-Cunico Sara Arnaboldi Barbara Bertasi Niccolò Franceschi Paolo Ajmone-Marsan Dario De Medici Marina Nadia Losio
container_volume	10
class	TP1-1185
format_se	Elektronische Aufsätze
author-letter	Andrea Zendrini
doi_str_mv	10.3390/foods10051132
author2-role	verfasserin
title_sort	one-day molecular detection of <i<salmonella</i< and <i<campylobacter</i< in chicken meat: a pilot study
callnumber	TP1-1185
title_auth	One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study
abstract	<i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation.
abstractGer	<i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation.
abstract_unstemmed	<i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	5, p 1132
title_short	One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study
url	https://doi.org/10.3390/foods10051132 https://doaj.org/article/510672a7632441f8866fd651eb869c6f https://www.mdpi.com/2304-8158/10/5/1132 https://doaj.org/toc/2304-8158
remote_bool	true
author2	Valentina Carta Virginia Filipello Laura Ragni Elena Cosciani-Cunico Sara Arnaboldi Barbara Bertasi Niccolò Franceschi Paolo Ajmone-Marsan Dario De Medici Marina Nadia Losio
author2Str	Valentina Carta Virginia Filipello Laura Ragni Elena Cosciani-Cunico Sara Arnaboldi Barbara Bertasi Niccolò Franceschi Paolo Ajmone-Marsan Dario De Medici Marina Nadia Losio
ppnlink	737287632
callnumber-subject	TP - Chemical Technology
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.3390/foods10051132
callnumber-a	TP1-1185
up_date	2024-07-03T18:00:27.526Z
_version_	1803581786715848704
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ00343947X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412180855.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230225s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/foods10051132</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ00343947X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ510672a7632441f8866fd651eb869c6f</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">TP1-1185</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Andrea Zendrini</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><i<Salmonella</i< and <i<Campylobacter</i< ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, <i<Salmonella</i< and <i<Campylobacter</i< screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18–24 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect <i<Salmonella</i< and <i<Campylobacter</i< in poultry meat contaminated at different concentration levels (10<sup<1</sup<, 10<sup<3</sup<, and 10<sup<5</sup< CFU/g). Our results show that real-time PCR allows the detection of <i<Salmonella</i< and <i<Campylobacter</i<, even after shorter enrichment times than prescribed by ISO references; particularly, it detected <i<Salmonella</i< down to 10<sup<1</sup< CFU/g since T0 and <i<Campylobacter</i< from 10<sup<3</sup< CFU/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">LAMP</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Campylobacter</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Salmonella</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">poultry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">foodborne diseases</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Chemical technology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Valentina Carta</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Virginia Filipello</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Laura Ragni</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Elena Cosciani-Cunico</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sara Arnaboldi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Barbara Bertasi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Niccolò Franceschi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Paolo Ajmone-Marsan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Dario De Medici</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Marina Nadia Losio</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Foods</subfield><subfield code="d">MDPI AG, 2013</subfield><subfield code="g">10(2021), 5, p 1132</subfield><subfield code="w">(DE-627)737287632</subfield><subfield code="w">(DE-600)2704223-6</subfield><subfield code="x">23048158</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:10</subfield><subfield code="g">year:2021</subfield><subfield code="g">number:5, p 1132</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/foods10051132</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/510672a7632441f8866fd651eb869c6f</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/2304-8158/10/5/1132</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2304-8158</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">10</subfield><subfield code="j">2021</subfield><subfield code="e">5, p 1132</subfield></datafield></record></collection>
score	7.401224

Nicht das Richtige dabei?

Schreiben Sie uns!

One-Day Molecular Detection of <i<Salmonella</i< and <i<Campylobacter</i< in Chicken Meat: A Pilot Study

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?