Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes

Homogenates of cultured hamster fibroblasts (BHK 21 cells) were fractionated by differential centrifugation into six main fractions: nuclear, mitochondrial, light mitochondrial, microsomal, soluble, and floating. The contents of several lipids and some marker enzymes were measured. According to the...
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Gespeichert in:

Autor*in:	J Brotherus [verfasserIn] O Renkonen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	1977

Schlagwörter:	differential centrifugation marker enzymes triglycerides cholesteryl esters lipid morphology

Übergeordnetes Werk:	In: Journal of Lipid Research - Elsevier, 2021, 18(1977), 2, Seite 191-202
Übergeordnetes Werk:	volume:18 ; year:1977 ; number:2 ; pages:191-202

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/S0022-2275(20)41698-0

Katalog-ID:	DOAJ004259912

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520			\|a Homogenates of cultured hamster fibroblasts (BHK 21 cells) were fractionated by differential centrifugation into six main fractions: nuclear, mitochondrial, light mitochondrial, microsomal, soluble, and floating. The contents of several lipids and some marker enzymes were measured. According to the enzyme distributions, lysosomes were enriched both in the floating fraction and in the light mitochondrial fraction. Lysobisphosphatidic acid was enriched in the floating fraction more than tenfold relative to phospholipid. Cholesteryl esters and triglycerides were the main constituents of the fraction (70% of total lipids). Lysobisphosphatidic acid, triglycerides, and cholesteryl esters were enriched also in the light mitochondrial fraction. Their distribution patterns were different from those of the other lipids. Electron microscopy showed that the floating fraction contained numerous lipofuscin-like particles with darkly stained peripheries and with core regions staining like droplets of neutral lipids. Similar particles, frequently containing prominent multilamellar formations, were also common in intact cells. They contained cytochemically identified acid phosphatase. We conclude that lysobisphosphatidic acid was enriched in the lysosomes of the BHK cells and that the lysosomes also contained variable amounts of neutral lipids in the form of intralysosomal droplets.
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spelling	10.1016/S0022-2275(20)41698-0 doi (DE-627)DOAJ004259912 (DE-599)DOAJ0659b9de5a58466bb6b3bc70be767f4f DE-627 ger DE-627 rakwb eng QD415-436 J Brotherus verfasserin aut Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes 1977 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Homogenates of cultured hamster fibroblasts (BHK 21 cells) were fractionated by differential centrifugation into six main fractions: nuclear, mitochondrial, light mitochondrial, microsomal, soluble, and floating. The contents of several lipids and some marker enzymes were measured. According to the enzyme distributions, lysosomes were enriched both in the floating fraction and in the light mitochondrial fraction. Lysobisphosphatidic acid was enriched in the floating fraction more than tenfold relative to phospholipid. Cholesteryl esters and triglycerides were the main constituents of the fraction (70% of total lipids). Lysobisphosphatidic acid, triglycerides, and cholesteryl esters were enriched also in the light mitochondrial fraction. Their distribution patterns were different from those of the other lipids. Electron microscopy showed that the floating fraction contained numerous lipofuscin-like particles with darkly stained peripheries and with core regions staining like droplets of neutral lipids. Similar particles, frequently containing prominent multilamellar formations, were also common in intact cells. They contained cytochemically identified acid phosphatase. We conclude that lysobisphosphatidic acid was enriched in the lysosomes of the BHK cells and that the lysosomes also contained variable amounts of neutral lipids in the form of intralysosomal droplets. differential centrifugation marker enzymes triglycerides cholesteryl esters lipid morphology Biochemistry O Renkonen verfasserin aut In Journal of Lipid Research Elsevier, 2021 18(1977), 2, Seite 191-202 (DE-627)26601593X (DE-600)1466675-3 15397262 nnns volume:18 year:1977 number:2 pages:191-202 https://doi.org/10.1016/S0022-2275(20)41698-0 kostenfrei https://doaj.org/article/0659b9de5a58466bb6b3bc70be767f4f kostenfrei http://www.sciencedirect.com/science/article/pii/S0022227520416980 kostenfrei https://doaj.org/toc/0022-2275 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 1977 2 191-202
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language	English
source	In Journal of Lipid Research 18(1977), 2, Seite 191-202 volume:18 year:1977 number:2 pages:191-202
sourceStr	In Journal of Lipid Research 18(1977), 2, Seite 191-202 volume:18 year:1977 number:2 pages:191-202
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	differential centrifugation marker enzymes triglycerides cholesteryl esters lipid morphology Biochemistry
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container_title	Journal of Lipid Research
authorswithroles_txt_mv	J Brotherus @@aut@@ O Renkonen @@aut@@
publishDateDaySort_date	1977-01-01T00:00:00Z
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callnumber-first	Q - Science
author	J Brotherus
spellingShingle	J Brotherus misc QD415-436 misc differential centrifugation misc marker enzymes misc triglycerides misc cholesteryl esters misc lipid morphology misc Biochemistry Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes
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topic_title	QD415-436 Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes differential centrifugation marker enzymes triglycerides cholesteryl esters lipid morphology
topic	misc QD415-436 misc differential centrifugation misc marker enzymes misc triglycerides misc cholesteryl esters misc lipid morphology misc Biochemistry
topic_unstemmed	misc QD415-436 misc differential centrifugation misc marker enzymes misc triglycerides misc cholesteryl esters misc lipid morphology misc Biochemistry
topic_browse	misc QD415-436 misc differential centrifugation misc marker enzymes misc triglycerides misc cholesteryl esters misc lipid morphology misc Biochemistry
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title	Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes
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title_full	Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes
author_sort	J Brotherus
journal	Journal of Lipid Research
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title_sort	subcellular distributions of lipids in cultured bhk cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes
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title_auth	Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes
abstract	Homogenates of cultured hamster fibroblasts (BHK 21 cells) were fractionated by differential centrifugation into six main fractions: nuclear, mitochondrial, light mitochondrial, microsomal, soluble, and floating. The contents of several lipids and some marker enzymes were measured. According to the enzyme distributions, lysosomes were enriched both in the floating fraction and in the light mitochondrial fraction. Lysobisphosphatidic acid was enriched in the floating fraction more than tenfold relative to phospholipid. Cholesteryl esters and triglycerides were the main constituents of the fraction (70% of total lipids). Lysobisphosphatidic acid, triglycerides, and cholesteryl esters were enriched also in the light mitochondrial fraction. Their distribution patterns were different from those of the other lipids. Electron microscopy showed that the floating fraction contained numerous lipofuscin-like particles with darkly stained peripheries and with core regions staining like droplets of neutral lipids. Similar particles, frequently containing prominent multilamellar formations, were also common in intact cells. They contained cytochemically identified acid phosphatase. We conclude that lysobisphosphatidic acid was enriched in the lysosomes of the BHK cells and that the lysosomes also contained variable amounts of neutral lipids in the form of intralysosomal droplets.
abstractGer	Homogenates of cultured hamster fibroblasts (BHK 21 cells) were fractionated by differential centrifugation into six main fractions: nuclear, mitochondrial, light mitochondrial, microsomal, soluble, and floating. The contents of several lipids and some marker enzymes were measured. According to the enzyme distributions, lysosomes were enriched both in the floating fraction and in the light mitochondrial fraction. Lysobisphosphatidic acid was enriched in the floating fraction more than tenfold relative to phospholipid. Cholesteryl esters and triglycerides were the main constituents of the fraction (70% of total lipids). Lysobisphosphatidic acid, triglycerides, and cholesteryl esters were enriched also in the light mitochondrial fraction. Their distribution patterns were different from those of the other lipids. Electron microscopy showed that the floating fraction contained numerous lipofuscin-like particles with darkly stained peripheries and with core regions staining like droplets of neutral lipids. Similar particles, frequently containing prominent multilamellar formations, were also common in intact cells. They contained cytochemically identified acid phosphatase. We conclude that lysobisphosphatidic acid was enriched in the lysosomes of the BHK cells and that the lysosomes also contained variable amounts of neutral lipids in the form of intralysosomal droplets.
abstract_unstemmed	Homogenates of cultured hamster fibroblasts (BHK 21 cells) were fractionated by differential centrifugation into six main fractions: nuclear, mitochondrial, light mitochondrial, microsomal, soluble, and floating. The contents of several lipids and some marker enzymes were measured. According to the enzyme distributions, lysosomes were enriched both in the floating fraction and in the light mitochondrial fraction. Lysobisphosphatidic acid was enriched in the floating fraction more than tenfold relative to phospholipid. Cholesteryl esters and triglycerides were the main constituents of the fraction (70% of total lipids). Lysobisphosphatidic acid, triglycerides, and cholesteryl esters were enriched also in the light mitochondrial fraction. Their distribution patterns were different from those of the other lipids. Electron microscopy showed that the floating fraction contained numerous lipofuscin-like particles with darkly stained peripheries and with core regions staining like droplets of neutral lipids. Similar particles, frequently containing prominent multilamellar formations, were also common in intact cells. They contained cytochemically identified acid phosphatase. We conclude that lysobisphosphatidic acid was enriched in the lysosomes of the BHK cells and that the lysosomes also contained variable amounts of neutral lipids in the form of intralysosomal droplets.
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title_short	Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes
url	https://doi.org/10.1016/S0022-2275(20)41698-0 https://doaj.org/article/0659b9de5a58466bb6b3bc70be767f4f http://www.sciencedirect.com/science/article/pii/S0022227520416980 https://doaj.org/toc/0022-2275
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Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes

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