In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates

Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is important for membrane prote...
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Autor*in:	Weirui Ma [verfasserIn] Gang Zhen [verfasserIn] Wei Xie [verfasserIn] Christine Mayr [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	RNA biology biomolecular condensates RNA multivalency in vivo reconstitution condensate morphology RNA-RNA interactions

Übergeordnetes Werk:	In: eLife - eLife Sciences Publications Ltd, 2013, 10(2021)
Übergeordnetes Werk:	volume:10 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.7554/eLife.64252

Katalog-ID:	DOAJ004383915

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520			\|a Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is important for membrane protein trafficking. It has been unclear how condensates with mesh-like shapes but dynamic protein components are formed. In vitro and in vivo reconstitution experiments revealed that the minimal components are a multivalent RNA-binding protein that concentrates RNAs that are able to form extensive intermolecular mRNA–mRNA interactions. mRNAs with large unstructured regions have a high propensity to form a pervasive intermolecular interaction network that acts as condensate skeleton. The underlying RNA matrix prevents full fusion of spherical liquid-like condensates, thus driving the formation of irregularly shaped membraneless organelles. The resulting large surface area may promote interactions at the condensate surface and at the interface with other organelles.
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spelling	10.7554/eLife.64252 doi (DE-627)DOAJ004383915 (DE-599)DOAJa09b87e8dcb5454298329c70f7480306 DE-627 ger DE-627 rakwb eng QH301-705.5 Weirui Ma verfasserin aut In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is important for membrane protein trafficking. It has been unclear how condensates with mesh-like shapes but dynamic protein components are formed. In vitro and in vivo reconstitution experiments revealed that the minimal components are a multivalent RNA-binding protein that concentrates RNAs that are able to form extensive intermolecular mRNA–mRNA interactions. mRNAs with large unstructured regions have a high propensity to form a pervasive intermolecular interaction network that acts as condensate skeleton. The underlying RNA matrix prevents full fusion of spherical liquid-like condensates, thus driving the formation of irregularly shaped membraneless organelles. The resulting large surface area may promote interactions at the condensate surface and at the interface with other organelles. RNA biology biomolecular condensates RNA multivalency in vivo reconstitution condensate morphology RNA-RNA interactions Medicine R Science Q Biology (General) Gang Zhen verfasserin aut Wei Xie verfasserin aut Christine Mayr verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 10(2021) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:10 year:2021 https://doi.org/10.7554/eLife.64252 kostenfrei https://doaj.org/article/a09b87e8dcb5454298329c70f7480306 kostenfrei https://elifesciences.org/articles/64252 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021
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allfieldsGer	10.7554/eLife.64252 doi (DE-627)DOAJ004383915 (DE-599)DOAJa09b87e8dcb5454298329c70f7480306 DE-627 ger DE-627 rakwb eng QH301-705.5 Weirui Ma verfasserin aut In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is important for membrane protein trafficking. It has been unclear how condensates with mesh-like shapes but dynamic protein components are formed. In vitro and in vivo reconstitution experiments revealed that the minimal components are a multivalent RNA-binding protein that concentrates RNAs that are able to form extensive intermolecular mRNA–mRNA interactions. mRNAs with large unstructured regions have a high propensity to form a pervasive intermolecular interaction network that acts as condensate skeleton. The underlying RNA matrix prevents full fusion of spherical liquid-like condensates, thus driving the formation of irregularly shaped membraneless organelles. The resulting large surface area may promote interactions at the condensate surface and at the interface with other organelles. RNA biology biomolecular condensates RNA multivalency in vivo reconstitution condensate morphology RNA-RNA interactions Medicine R Science Q Biology (General) Gang Zhen verfasserin aut Wei Xie verfasserin aut Christine Mayr verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 10(2021) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:10 year:2021 https://doi.org/10.7554/eLife.64252 kostenfrei https://doaj.org/article/a09b87e8dcb5454298329c70f7480306 kostenfrei https://elifesciences.org/articles/64252 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021
allfieldsSound	10.7554/eLife.64252 doi (DE-627)DOAJ004383915 (DE-599)DOAJa09b87e8dcb5454298329c70f7480306 DE-627 ger DE-627 rakwb eng QH301-705.5 Weirui Ma verfasserin aut In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is important for membrane protein trafficking. It has been unclear how condensates with mesh-like shapes but dynamic protein components are formed. In vitro and in vivo reconstitution experiments revealed that the minimal components are a multivalent RNA-binding protein that concentrates RNAs that are able to form extensive intermolecular mRNA–mRNA interactions. mRNAs with large unstructured regions have a high propensity to form a pervasive intermolecular interaction network that acts as condensate skeleton. The underlying RNA matrix prevents full fusion of spherical liquid-like condensates, thus driving the formation of irregularly shaped membraneless organelles. The resulting large surface area may promote interactions at the condensate surface and at the interface with other organelles. RNA biology biomolecular condensates RNA multivalency in vivo reconstitution condensate morphology RNA-RNA interactions Medicine R Science Q Biology (General) Gang Zhen verfasserin aut Wei Xie verfasserin aut Christine Mayr verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 10(2021) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:10 year:2021 https://doi.org/10.7554/eLife.64252 kostenfrei https://doaj.org/article/a09b87e8dcb5454298329c70f7480306 kostenfrei https://elifesciences.org/articles/64252 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021
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topic_facet	RNA biology biomolecular condensates RNA multivalency in vivo reconstitution condensate morphology RNA-RNA interactions Medicine R Science Q Biology (General)
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container_title	eLife
authorswithroles_txt_mv	Weirui Ma @@aut@@ Gang Zhen @@aut@@ Wei Xie @@aut@@ Christine Mayr @@aut@@
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callnumber-first	Q - Science
author	Weirui Ma
spellingShingle	Weirui Ma misc QH301-705.5 misc RNA biology misc biomolecular condensates misc RNA multivalency misc in vivo reconstitution misc condensate morphology misc RNA-RNA interactions misc Medicine misc R misc Science misc Q misc Biology (General) In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates
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topic_title	QH301-705.5 In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates RNA biology biomolecular condensates RNA multivalency in vivo reconstitution condensate morphology RNA-RNA interactions
topic	misc QH301-705.5 misc RNA biology misc biomolecular condensates misc RNA multivalency misc in vivo reconstitution misc condensate morphology misc RNA-RNA interactions misc Medicine misc R misc Science misc Q misc Biology (General)
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title	In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates
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title_full	In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates
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title_auth	In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates
abstract	Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is important for membrane protein trafficking. It has been unclear how condensates with mesh-like shapes but dynamic protein components are formed. In vitro and in vivo reconstitution experiments revealed that the minimal components are a multivalent RNA-binding protein that concentrates RNAs that are able to form extensive intermolecular mRNA–mRNA interactions. mRNAs with large unstructured regions have a high propensity to form a pervasive intermolecular interaction network that acts as condensate skeleton. The underlying RNA matrix prevents full fusion of spherical liquid-like condensates, thus driving the formation of irregularly shaped membraneless organelles. The resulting large surface area may promote interactions at the condensate surface and at the interface with other organelles.
abstractGer	Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is important for membrane protein trafficking. It has been unclear how condensates with mesh-like shapes but dynamic protein components are formed. In vitro and in vivo reconstitution experiments revealed that the minimal components are a multivalent RNA-binding protein that concentrates RNAs that are able to form extensive intermolecular mRNA–mRNA interactions. mRNAs with large unstructured regions have a high propensity to form a pervasive intermolecular interaction network that acts as condensate skeleton. The underlying RNA matrix prevents full fusion of spherical liquid-like condensates, thus driving the formation of irregularly shaped membraneless organelles. The resulting large surface area may promote interactions at the condensate surface and at the interface with other organelles.
abstract_unstemmed	Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is important for membrane protein trafficking. It has been unclear how condensates with mesh-like shapes but dynamic protein components are formed. In vitro and in vivo reconstitution experiments revealed that the minimal components are a multivalent RNA-binding protein that concentrates RNAs that are able to form extensive intermolecular mRNA–mRNA interactions. mRNAs with large unstructured regions have a high propensity to form a pervasive intermolecular interaction network that acts as condensate skeleton. The underlying RNA matrix prevents full fusion of spherical liquid-like condensates, thus driving the formation of irregularly shaped membraneless organelles. The resulting large surface area may promote interactions at the condensate surface and at the interface with other organelles.
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title_short	In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates
url	https://doi.org/10.7554/eLife.64252 https://doaj.org/article/a09b87e8dcb5454298329c70f7480306 https://elifesciences.org/articles/64252 https://doaj.org/toc/2050-084X
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up_date	2024-07-03T23:28:22.763Z
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