Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation

Virus propagation methods generally use transformed cell lines to grow viruses from clinical specimens, which may force viruses to rapidly adapt to cell culture conditions, a process facilitated by high viral mutation rates. Upon propagation in VeroE6 cells, SARS-CoV-2 may mutate or delete the multi...
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Autor*in:	Mart M Lamers [verfasserIn] Anna Z Mykytyn [verfasserIn] Tim I Breugem [verfasserIn] Yiquan Wang [verfasserIn] Douglas C Wu [verfasserIn] Samra Riesebosch [verfasserIn] Petra B van den Doel [verfasserIn] Debby Schipper [verfasserIn] Theo Bestebroer [verfasserIn] Nicholas C Wu [verfasserIn] Bart L Haagmans [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	COVID-19 SARS-CoV-2 cell culture adaptation furin cleavage site serine proteases airway organoids

Übergeordnetes Werk:	In: eLife - eLife Sciences Publications Ltd, 2013, 10(2021)
Übergeordnetes Werk:	volume:10 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.7554/eLife.66815

Katalog-ID:	DOAJ004407407

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allfieldsSound	10.7554/eLife.66815 doi (DE-627)DOAJ004407407 (DE-599)DOAJ3d0c2b13478f4404ae22e956acd8b062 DE-627 ger DE-627 rakwb eng QH301-705.5 Mart M Lamers verfasserin aut Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Virus propagation methods generally use transformed cell lines to grow viruses from clinical specimens, which may force viruses to rapidly adapt to cell culture conditions, a process facilitated by high viral mutation rates. Upon propagation in VeroE6 cells, SARS-CoV-2 may mutate or delete the multibasic cleavage site (MBCS) in the spike protein. Previously, we showed that the MBCS facilitates serine protease-mediated entry into human airway cells (Mykytyn et al., 2021). Here, we report that propagating SARS-CoV-2 on the human airway cell line Calu-3 – that expresses serine proteases – prevents cell culture adaptations in the MBCS and directly adjacent to the MBCS (S686G). Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation. Thus, in-depth knowledge on the biology of a virus can be used to establish methods to prevent cell culture adaptation. COVID-19 SARS-CoV-2 cell culture adaptation furin cleavage site serine proteases airway organoids Medicine R Science Q Biology (General) Anna Z Mykytyn verfasserin aut Tim I Breugem verfasserin aut Yiquan Wang verfasserin aut Douglas C Wu verfasserin aut Samra Riesebosch verfasserin aut Petra B van den Doel verfasserin aut Debby Schipper verfasserin aut Theo Bestebroer verfasserin aut Nicholas C Wu verfasserin aut Bart L Haagmans verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 10(2021) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:10 year:2021 https://doi.org/10.7554/eLife.66815 kostenfrei https://doaj.org/article/3d0c2b13478f4404ae22e956acd8b062 kostenfrei https://elifesciences.org/articles/66815 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021
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topic_facet	COVID-19 SARS-CoV-2 cell culture adaptation furin cleavage site serine proteases airway organoids Medicine R Science Q Biology (General)
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authorswithroles_txt_mv	Mart M Lamers @@aut@@ Anna Z Mykytyn @@aut@@ Tim I Breugem @@aut@@ Yiquan Wang @@aut@@ Douglas C Wu @@aut@@ Samra Riesebosch @@aut@@ Petra B van den Doel @@aut@@ Debby Schipper @@aut@@ Theo Bestebroer @@aut@@ Nicholas C Wu @@aut@@ Bart L Haagmans @@aut@@
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callnumber-first	Q - Science
author	Mart M Lamers
spellingShingle	Mart M Lamers misc QH301-705.5 misc COVID-19 misc SARS-CoV-2 misc cell culture adaptation misc furin cleavage site misc serine proteases misc airway organoids misc Medicine misc R misc Science misc Q misc Biology (General) Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation
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topic_title	QH301-705.5 Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation COVID-19 SARS-CoV-2 cell culture adaptation furin cleavage site serine proteases airway organoids
topic	misc QH301-705.5 misc COVID-19 misc SARS-CoV-2 misc cell culture adaptation misc furin cleavage site misc serine proteases misc airway organoids misc Medicine misc R misc Science misc Q misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc COVID-19 misc SARS-CoV-2 misc cell culture adaptation misc furin cleavage site misc serine proteases misc airway organoids misc Medicine misc R misc Science misc Q misc Biology (General)
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title	Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation
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title_full	Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation
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title_sort	human airway cells prevent sars-cov-2 multibasic cleavage site cell culture adaptation
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title_auth	Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation
abstract	Virus propagation methods generally use transformed cell lines to grow viruses from clinical specimens, which may force viruses to rapidly adapt to cell culture conditions, a process facilitated by high viral mutation rates. Upon propagation in VeroE6 cells, SARS-CoV-2 may mutate or delete the multibasic cleavage site (MBCS) in the spike protein. Previously, we showed that the MBCS facilitates serine protease-mediated entry into human airway cells (Mykytyn et al., 2021). Here, we report that propagating SARS-CoV-2 on the human airway cell line Calu-3 – that expresses serine proteases – prevents cell culture adaptations in the MBCS and directly adjacent to the MBCS (S686G). Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation. Thus, in-depth knowledge on the biology of a virus can be used to establish methods to prevent cell culture adaptation.
abstractGer	Virus propagation methods generally use transformed cell lines to grow viruses from clinical specimens, which may force viruses to rapidly adapt to cell culture conditions, a process facilitated by high viral mutation rates. Upon propagation in VeroE6 cells, SARS-CoV-2 may mutate or delete the multibasic cleavage site (MBCS) in the spike protein. Previously, we showed that the MBCS facilitates serine protease-mediated entry into human airway cells (Mykytyn et al., 2021). Here, we report that propagating SARS-CoV-2 on the human airway cell line Calu-3 – that expresses serine proteases – prevents cell culture adaptations in the MBCS and directly adjacent to the MBCS (S686G). Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation. Thus, in-depth knowledge on the biology of a virus can be used to establish methods to prevent cell culture adaptation.
abstract_unstemmed	Virus propagation methods generally use transformed cell lines to grow viruses from clinical specimens, which may force viruses to rapidly adapt to cell culture conditions, a process facilitated by high viral mutation rates. Upon propagation in VeroE6 cells, SARS-CoV-2 may mutate or delete the multibasic cleavage site (MBCS) in the spike protein. Previously, we showed that the MBCS facilitates serine protease-mediated entry into human airway cells (Mykytyn et al., 2021). Here, we report that propagating SARS-CoV-2 on the human airway cell line Calu-3 – that expresses serine proteases – prevents cell culture adaptations in the MBCS and directly adjacent to the MBCS (S686G). Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation. Thus, in-depth knowledge on the biology of a virus can be used to establish methods to prevent cell culture adaptation.
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title_short	Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation
url	https://doi.org/10.7554/eLife.66815 https://doaj.org/article/3d0c2b13478f4404ae22e956acd8b062 https://elifesciences.org/articles/66815 https://doaj.org/toc/2050-084X
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up_date	2024-07-03T23:34:43.614Z
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Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation

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