Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC
Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection o...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
LIU Jiaxin [verfasserIn] LI Shanshan, [verfasserIn] WANG Dong [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Chinesisch |
| Erschienen: |
2022 |
|---|
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
In: 陆军军医大学学报 - Editorial Office of Journal of Army Medical University, 2022, 44(2022), 24, Seite 2500-2506 |
|---|---|
| Übergeordnetes Werk: |
volume:44 ; year:2022 ; number:24 ; pages:2500-2506 |
| Links: |
|---|
| DOI / URN: |
10.16016/j.2097-0927.202209006 |
|---|
| Katalog-ID: |
DOAJ004494237 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | DOAJ004494237 | ||
| 003 | DE-627 | ||
| 005 | 20230311013829.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230225s2022 xx |||||o 00| ||chi c | ||
| 024 | 7 | |a 10.16016/j.2097-0927.202209006 |2 doi | |
| 035 | |a (DE-627)DOAJ004494237 | ||
| 035 | |a (DE-599)DOAJ43e7f0c7dd4b4ddc88e6ea2c745e6824 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a chi | ||
| 050 | 0 | |a R5-920 | |
| 100 | 0 | |a LIU Jiaxin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process. | ||
| 650 | 4 | |a alk targeted therapy | |
| 650 | 4 | |a crizotinib | |
| 650 | 4 | |a resistance | |
| 650 | 4 | |a murine double minute 2 | |
| 650 | 4 | |a epithelial mesenchymal transition | |
| 653 | 0 | |a Medicine (General) | |
| 700 | 0 | |a LI Shanshan, |e verfasserin |4 aut | |
| 700 | 0 | |a WANG Dong |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i In |t 陆军军医大学学报 |d Editorial Office of Journal of Army Medical University, 2022 |g 44(2022), 24, Seite 2500-2506 |w (DE-627)DOAJ078594804 |x 20970927 |7 nnns |
| 773 | 1 | 8 | |g volume:44 |g year:2022 |g number:24 |g pages:2500-2506 |
| 856 | 4 | 0 | |u https://doi.org/10.16016/j.2097-0927.202209006 |z kostenfrei |
| 856 | 4 | 0 | |u https://doaj.org/article/43e7f0c7dd4b4ddc88e6ea2c745e6824 |z kostenfrei |
| 856 | 4 | 0 | |u http://aammt.tmmu.edu.cn/Upload/rhtml/202209006.htm |z kostenfrei |
| 856 | 4 | 2 | |u https://doaj.org/toc/2097-0927 |y Journal toc |z kostenfrei |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_DOAJ | ||
| 951 | |a AR | ||
| 952 | |d 44 |j 2022 |e 24 |h 2500-2506 | ||
| author_variant |
l j lj l s ls w d wd |
|---|---|
| matchkey_str |
article:20970927:2022----::vrxrsinfd2nuecioiirssacitreete |
| hierarchy_sort_str |
2022 |
| callnumber-subject-code |
R |
| publishDate |
2022 |
| allfields |
10.16016/j.2097-0927.202209006 doi (DE-627)DOAJ004494237 (DE-599)DOAJ43e7f0c7dd4b4ddc88e6ea2c745e6824 DE-627 ger DE-627 rakwb chi R5-920 LIU Jiaxin verfasserin aut Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process. alk targeted therapy crizotinib resistance murine double minute 2 epithelial mesenchymal transition Medicine (General) LI Shanshan, verfasserin aut WANG Dong verfasserin aut In 陆军军医大学学报 Editorial Office of Journal of Army Medical University, 2022 44(2022), 24, Seite 2500-2506 (DE-627)DOAJ078594804 20970927 nnns volume:44 year:2022 number:24 pages:2500-2506 https://doi.org/10.16016/j.2097-0927.202209006 kostenfrei https://doaj.org/article/43e7f0c7dd4b4ddc88e6ea2c745e6824 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/202209006.htm kostenfrei https://doaj.org/toc/2097-0927 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 44 2022 24 2500-2506 |
| spelling |
10.16016/j.2097-0927.202209006 doi (DE-627)DOAJ004494237 (DE-599)DOAJ43e7f0c7dd4b4ddc88e6ea2c745e6824 DE-627 ger DE-627 rakwb chi R5-920 LIU Jiaxin verfasserin aut Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process. alk targeted therapy crizotinib resistance murine double minute 2 epithelial mesenchymal transition Medicine (General) LI Shanshan, verfasserin aut WANG Dong verfasserin aut In 陆军军医大学学报 Editorial Office of Journal of Army Medical University, 2022 44(2022), 24, Seite 2500-2506 (DE-627)DOAJ078594804 20970927 nnns volume:44 year:2022 number:24 pages:2500-2506 https://doi.org/10.16016/j.2097-0927.202209006 kostenfrei https://doaj.org/article/43e7f0c7dd4b4ddc88e6ea2c745e6824 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/202209006.htm kostenfrei https://doaj.org/toc/2097-0927 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 44 2022 24 2500-2506 |
| allfields_unstemmed |
10.16016/j.2097-0927.202209006 doi (DE-627)DOAJ004494237 (DE-599)DOAJ43e7f0c7dd4b4ddc88e6ea2c745e6824 DE-627 ger DE-627 rakwb chi R5-920 LIU Jiaxin verfasserin aut Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process. alk targeted therapy crizotinib resistance murine double minute 2 epithelial mesenchymal transition Medicine (General) LI Shanshan, verfasserin aut WANG Dong verfasserin aut In 陆军军医大学学报 Editorial Office of Journal of Army Medical University, 2022 44(2022), 24, Seite 2500-2506 (DE-627)DOAJ078594804 20970927 nnns volume:44 year:2022 number:24 pages:2500-2506 https://doi.org/10.16016/j.2097-0927.202209006 kostenfrei https://doaj.org/article/43e7f0c7dd4b4ddc88e6ea2c745e6824 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/202209006.htm kostenfrei https://doaj.org/toc/2097-0927 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 44 2022 24 2500-2506 |
| allfieldsGer |
10.16016/j.2097-0927.202209006 doi (DE-627)DOAJ004494237 (DE-599)DOAJ43e7f0c7dd4b4ddc88e6ea2c745e6824 DE-627 ger DE-627 rakwb chi R5-920 LIU Jiaxin verfasserin aut Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process. alk targeted therapy crizotinib resistance murine double minute 2 epithelial mesenchymal transition Medicine (General) LI Shanshan, verfasserin aut WANG Dong verfasserin aut In 陆军军医大学学报 Editorial Office of Journal of Army Medical University, 2022 44(2022), 24, Seite 2500-2506 (DE-627)DOAJ078594804 20970927 nnns volume:44 year:2022 number:24 pages:2500-2506 https://doi.org/10.16016/j.2097-0927.202209006 kostenfrei https://doaj.org/article/43e7f0c7dd4b4ddc88e6ea2c745e6824 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/202209006.htm kostenfrei https://doaj.org/toc/2097-0927 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 44 2022 24 2500-2506 |
| allfieldsSound |
10.16016/j.2097-0927.202209006 doi (DE-627)DOAJ004494237 (DE-599)DOAJ43e7f0c7dd4b4ddc88e6ea2c745e6824 DE-627 ger DE-627 rakwb chi R5-920 LIU Jiaxin verfasserin aut Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process. alk targeted therapy crizotinib resistance murine double minute 2 epithelial mesenchymal transition Medicine (General) LI Shanshan, verfasserin aut WANG Dong verfasserin aut In 陆军军医大学学报 Editorial Office of Journal of Army Medical University, 2022 44(2022), 24, Seite 2500-2506 (DE-627)DOAJ078594804 20970927 nnns volume:44 year:2022 number:24 pages:2500-2506 https://doi.org/10.16016/j.2097-0927.202209006 kostenfrei https://doaj.org/article/43e7f0c7dd4b4ddc88e6ea2c745e6824 kostenfrei http://aammt.tmmu.edu.cn/Upload/rhtml/202209006.htm kostenfrei https://doaj.org/toc/2097-0927 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 44 2022 24 2500-2506 |
| language |
Chinese |
| source |
In 陆军军医大学学报 44(2022), 24, Seite 2500-2506 volume:44 year:2022 number:24 pages:2500-2506 |
| sourceStr |
In 陆军军医大学学报 44(2022), 24, Seite 2500-2506 volume:44 year:2022 number:24 pages:2500-2506 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
alk targeted therapy crizotinib resistance murine double minute 2 epithelial mesenchymal transition Medicine (General) |
| isfreeaccess_bool |
true |
| container_title |
陆军军医大学学报 |
| authorswithroles_txt_mv |
LIU Jiaxin @@aut@@ LI Shanshan, @@aut@@ WANG Dong @@aut@@ |
| publishDateDaySort_date |
2022-01-01T00:00:00Z |
| hierarchy_top_id |
DOAJ078594804 |
| id |
DOAJ004494237 |
| language_de |
chinesisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ004494237</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230311013829.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230225s2022 xx |||||o 00| ||chi c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.16016/j.2097-0927.202209006</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ004494237</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ43e7f0c7dd4b4ddc88e6ea2c745e6824</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">chi</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">R5-920</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">LIU Jiaxin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">alk targeted therapy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">crizotinib</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">resistance</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">murine double minute 2</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">epithelial mesenchymal transition</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">LI Shanshan,</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">WANG Dong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">陆军军医大学学报</subfield><subfield code="d">Editorial Office of Journal of Army Medical University, 2022</subfield><subfield code="g">44(2022), 24, Seite 2500-2506</subfield><subfield code="w">(DE-627)DOAJ078594804</subfield><subfield code="x">20970927</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:44</subfield><subfield code="g">year:2022</subfield><subfield code="g">number:24</subfield><subfield code="g">pages:2500-2506</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.16016/j.2097-0927.202209006</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/43e7f0c7dd4b4ddc88e6ea2c745e6824</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://aammt.tmmu.edu.cn/Upload/rhtml/202209006.htm</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2097-0927</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">44</subfield><subfield code="j">2022</subfield><subfield code="e">24</subfield><subfield code="h">2500-2506</subfield></datafield></record></collection>
|
| callnumber-first |
R - Medicine |
| author |
LIU Jiaxin |
| spellingShingle |
LIU Jiaxin misc R5-920 misc alk targeted therapy misc crizotinib misc resistance misc murine double minute 2 misc epithelial mesenchymal transition misc Medicine (General) Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC |
| authorStr |
LIU Jiaxin |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)DOAJ078594804 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut |
| collection |
DOAJ |
| remote_str |
true |
| callnumber-label |
R5-920 |
| illustrated |
Not Illustrated |
| issn |
20970927 |
| topic_title |
R5-920 Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC alk targeted therapy crizotinib resistance murine double minute 2 epithelial mesenchymal transition |
| topic |
misc R5-920 misc alk targeted therapy misc crizotinib misc resistance misc murine double minute 2 misc epithelial mesenchymal transition misc Medicine (General) |
| topic_unstemmed |
misc R5-920 misc alk targeted therapy misc crizotinib misc resistance misc murine double minute 2 misc epithelial mesenchymal transition misc Medicine (General) |
| topic_browse |
misc R5-920 misc alk targeted therapy misc crizotinib misc resistance misc murine double minute 2 misc epithelial mesenchymal transition misc Medicine (General) |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| hierarchy_parent_title |
陆军军医大学学报 |
| hierarchy_parent_id |
DOAJ078594804 |
| hierarchy_top_title |
陆军军医大学学报 |
| isfreeaccess_txt |
true |
| familylinks_str_mv |
(DE-627)DOAJ078594804 |
| title |
Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC |
| ctrlnum |
(DE-627)DOAJ004494237 (DE-599)DOAJ43e7f0c7dd4b4ddc88e6ea2c745e6824 |
| title_full |
Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC |
| author_sort |
LIU Jiaxin |
| journal |
陆军军医大学学报 |
| journalStr |
陆军军医大学学报 |
| callnumber-first-code |
R |
| lang_code |
chi |
| isOA_bool |
true |
| recordtype |
marc |
| publishDateSort |
2022 |
| contenttype_str_mv |
txt |
| container_start_page |
2500 |
| author_browse |
LIU Jiaxin LI Shanshan, WANG Dong |
| container_volume |
44 |
| class |
R5-920 |
| format_se |
Elektronische Aufsätze |
| author-letter |
LIU Jiaxin |
| doi_str_mv |
10.16016/j.2097-0927.202209006 |
| author2-role |
verfasserin |
| title_sort |
overexpression of mdm2 induces crizotinib resistance in targeted therapy for alk-positive nsclc |
| callnumber |
R5-920 |
| title_auth |
Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC |
| abstract |
Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process. |
| abstractGer |
Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process. |
| abstract_unstemmed |
Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process. |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ |
| container_issue |
24 |
| title_short |
Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC |
| url |
https://doi.org/10.16016/j.2097-0927.202209006 https://doaj.org/article/43e7f0c7dd4b4ddc88e6ea2c745e6824 http://aammt.tmmu.edu.cn/Upload/rhtml/202209006.htm https://doaj.org/toc/2097-0927 |
| remote_bool |
true |
| author2 |
LI Shanshan WANG Dong |
| author2Str |
LI Shanshan WANG Dong |
| ppnlink |
DOAJ078594804 |
| callnumber-subject |
R - General Medicine |
| mediatype_str_mv |
c |
| isOA_txt |
true |
| hochschulschrift_bool |
false |
| doi_str |
10.16016/j.2097-0927.202209006 |
| callnumber-a |
R5-920 |
| up_date |
2024-07-03T23:57:51.010Z |
| _version_ |
1803604271836430336 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ004494237</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230311013829.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230225s2022 xx |||||o 00| ||chi c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.16016/j.2097-0927.202209006</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ004494237</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ43e7f0c7dd4b4ddc88e6ea2c745e6824</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">chi</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">R5-920</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">LIU Jiaxin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objective To investigate the effect of overexpression of murine double minute 2 (Mdm2) on the sensitivity to crizotinib in the anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) cell line H3122. Methods H3122 cells with Mdm2 overexpression were established by transfection of plasmid encoding Mdm2, and the H3122 cells transfected with empty vector (EV) served as control cells. After the Mdm2 overexpression cells and control cells were treated with different concentrations of crizotinib, an ALK inhibitor, MTT assay, colony formation assay and TUNEL assay were applied to evaluate the sensitivity to crizotinib. Western blotting was used to detect the expression of ALK, its downstream proteins and biomarkers in epithelial mesenchymal transition (EMT). Results Compared with the control cells, the H3122 cells with Mdm2 overexpression were resistant to crizotinib treatment, and were tolerant to crizotinib-induced apoptosis. Biochemical analysis of signaling transduction suggested that overexpression of Mdm2 failed to restore the phosphorylation of ALK and downstream molecules. Overexpression of Mdm2 also resulted in EMT and acquisition of mesenchymal phenotype in H3122 cells. Conclusion Overexpression of Mdm2 leads to resistance to crizotinib in H3122 cells probably through the induction of EMT process.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">alk targeted therapy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">crizotinib</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">resistance</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">murine double minute 2</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">epithelial mesenchymal transition</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">LI Shanshan,</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">WANG Dong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">陆军军医大学学报</subfield><subfield code="d">Editorial Office of Journal of Army Medical University, 2022</subfield><subfield code="g">44(2022), 24, Seite 2500-2506</subfield><subfield code="w">(DE-627)DOAJ078594804</subfield><subfield code="x">20970927</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:44</subfield><subfield code="g">year:2022</subfield><subfield code="g">number:24</subfield><subfield code="g">pages:2500-2506</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.16016/j.2097-0927.202209006</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/43e7f0c7dd4b4ddc88e6ea2c745e6824</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://aammt.tmmu.edu.cn/Upload/rhtml/202209006.htm</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2097-0927</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">44</subfield><subfield code="j">2022</subfield><subfield code="e">24</subfield><subfield code="h">2500-2506</subfield></datafield></record></collection>
|
| score |
7.402316 |