Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus

The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm e...
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Autor*in:	Zhengqiang Chen [verfasserIn] Qian Wu [verfasserIn] Cong Tong [verfasserIn] Hongyu Chen [verfasserIn] Dan Miao [verfasserIn] Xin Qian [verfasserIn] Xiaohui Zhao [verfasserIn] Lei Jiang [verfasserIn] Xiaorong Tao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	tomato spotted wilt virus NLR receptor plant innate immunity defense signaling

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 13(2021), 8, p 1447
Übergeordnetes Werk:	volume:13 ; year:2021 ; number:8, p 1447

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v13081447

Katalog-ID:	DOAJ005193672

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520			\|a The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm elicitor has increased significantly, the process by which <i<Sw-5b</i< activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the <i<Sw-5b</i<-mediated signaling pathway. We found that chaperone SGT1 was required for <i<Sw-5b</i< function, but co-chaperone RAR1 was not. <i<Sw-5b</i<-mediated immune signaling was independent of both EDS1 and NDR1. Silencing <i<NPR1</i<, which is a central component in SA signaling, did not result in TSWV systemic infection in <i<Sw-5b</i<-transgenic <i<N. benthamiana</i< plants. Helper NLR NRCs (NLRs required for cell death) were required for <i<Sw-5b</i<-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the <i<Sw-5b</i< resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the <i<Sw-5b</i< signaling pathway. Silencing ADR1, NRG1, or both genes did not affect <i<Sw-5b</i<-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in <i<Sw-5b</i<-mediated signaling pathways.
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allfields	10.3390/v13081447 doi (DE-627)DOAJ005193672 (DE-599)DOAJ6121581466974b0fafe204fa3edad499 DE-627 ger DE-627 rakwb eng QR1-502 Zhengqiang Chen verfasserin aut Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm elicitor has increased significantly, the process by which <i<Sw-5b</i< activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the <i<Sw-5b</i<-mediated signaling pathway. We found that chaperone SGT1 was required for <i<Sw-5b</i< function, but co-chaperone RAR1 was not. <i<Sw-5b</i<-mediated immune signaling was independent of both EDS1 and NDR1. Silencing <i<NPR1</i<, which is a central component in SA signaling, did not result in TSWV systemic infection in <i<Sw-5b</i<-transgenic <i<N. benthamiana</i< plants. Helper NLR NRCs (NLRs required for cell death) were required for <i<Sw-5b</i<-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the <i<Sw-5b</i< resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the <i<Sw-5b</i< signaling pathway. Silencing ADR1, NRG1, or both genes did not affect <i<Sw-5b</i<-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in <i<Sw-5b</i<-mediated signaling pathways. tomato spotted wilt virus <i<Sw-5b</i< NLR receptor plant innate immunity defense signaling Microbiology Qian Wu verfasserin aut Cong Tong verfasserin aut Hongyu Chen verfasserin aut Dan Miao verfasserin aut Xin Qian verfasserin aut Xiaohui Zhao verfasserin aut Lei Jiang verfasserin aut Xiaorong Tao verfasserin aut In Viruses MDPI AG, 2009 13(2021), 8, p 1447 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:13 year:2021 number:8, p 1447 https://doi.org/10.3390/v13081447 kostenfrei https://doaj.org/article/6121581466974b0fafe204fa3edad499 kostenfrei https://www.mdpi.com/1999-4915/13/8/1447 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 8, p 1447
spelling	10.3390/v13081447 doi (DE-627)DOAJ005193672 (DE-599)DOAJ6121581466974b0fafe204fa3edad499 DE-627 ger DE-627 rakwb eng QR1-502 Zhengqiang Chen verfasserin aut Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm elicitor has increased significantly, the process by which <i<Sw-5b</i< activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the <i<Sw-5b</i<-mediated signaling pathway. We found that chaperone SGT1 was required for <i<Sw-5b</i< function, but co-chaperone RAR1 was not. <i<Sw-5b</i<-mediated immune signaling was independent of both EDS1 and NDR1. Silencing <i<NPR1</i<, which is a central component in SA signaling, did not result in TSWV systemic infection in <i<Sw-5b</i<-transgenic <i<N. benthamiana</i< plants. Helper NLR NRCs (NLRs required for cell death) were required for <i<Sw-5b</i<-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the <i<Sw-5b</i< resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the <i<Sw-5b</i< signaling pathway. Silencing ADR1, NRG1, or both genes did not affect <i<Sw-5b</i<-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in <i<Sw-5b</i<-mediated signaling pathways. tomato spotted wilt virus <i<Sw-5b</i< NLR receptor plant innate immunity defense signaling Microbiology Qian Wu verfasserin aut Cong Tong verfasserin aut Hongyu Chen verfasserin aut Dan Miao verfasserin aut Xin Qian verfasserin aut Xiaohui Zhao verfasserin aut Lei Jiang verfasserin aut Xiaorong Tao verfasserin aut In Viruses MDPI AG, 2009 13(2021), 8, p 1447 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:13 year:2021 number:8, p 1447 https://doi.org/10.3390/v13081447 kostenfrei https://doaj.org/article/6121581466974b0fafe204fa3edad499 kostenfrei https://www.mdpi.com/1999-4915/13/8/1447 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 8, p 1447
allfields_unstemmed	10.3390/v13081447 doi (DE-627)DOAJ005193672 (DE-599)DOAJ6121581466974b0fafe204fa3edad499 DE-627 ger DE-627 rakwb eng QR1-502 Zhengqiang Chen verfasserin aut Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm elicitor has increased significantly, the process by which <i<Sw-5b</i< activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the <i<Sw-5b</i<-mediated signaling pathway. We found that chaperone SGT1 was required for <i<Sw-5b</i< function, but co-chaperone RAR1 was not. <i<Sw-5b</i<-mediated immune signaling was independent of both EDS1 and NDR1. Silencing <i<NPR1</i<, which is a central component in SA signaling, did not result in TSWV systemic infection in <i<Sw-5b</i<-transgenic <i<N. benthamiana</i< plants. Helper NLR NRCs (NLRs required for cell death) were required for <i<Sw-5b</i<-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the <i<Sw-5b</i< resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the <i<Sw-5b</i< signaling pathway. Silencing ADR1, NRG1, or both genes did not affect <i<Sw-5b</i<-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in <i<Sw-5b</i<-mediated signaling pathways. tomato spotted wilt virus <i<Sw-5b</i< NLR receptor plant innate immunity defense signaling Microbiology Qian Wu verfasserin aut Cong Tong verfasserin aut Hongyu Chen verfasserin aut Dan Miao verfasserin aut Xin Qian verfasserin aut Xiaohui Zhao verfasserin aut Lei Jiang verfasserin aut Xiaorong Tao verfasserin aut In Viruses MDPI AG, 2009 13(2021), 8, p 1447 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:13 year:2021 number:8, p 1447 https://doi.org/10.3390/v13081447 kostenfrei https://doaj.org/article/6121581466974b0fafe204fa3edad499 kostenfrei https://www.mdpi.com/1999-4915/13/8/1447 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 8, p 1447
allfieldsGer	10.3390/v13081447 doi (DE-627)DOAJ005193672 (DE-599)DOAJ6121581466974b0fafe204fa3edad499 DE-627 ger DE-627 rakwb eng QR1-502 Zhengqiang Chen verfasserin aut Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm elicitor has increased significantly, the process by which <i<Sw-5b</i< activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the <i<Sw-5b</i<-mediated signaling pathway. We found that chaperone SGT1 was required for <i<Sw-5b</i< function, but co-chaperone RAR1 was not. <i<Sw-5b</i<-mediated immune signaling was independent of both EDS1 and NDR1. Silencing <i<NPR1</i<, which is a central component in SA signaling, did not result in TSWV systemic infection in <i<Sw-5b</i<-transgenic <i<N. benthamiana</i< plants. Helper NLR NRCs (NLRs required for cell death) were required for <i<Sw-5b</i<-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the <i<Sw-5b</i< resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the <i<Sw-5b</i< signaling pathway. Silencing ADR1, NRG1, or both genes did not affect <i<Sw-5b</i<-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in <i<Sw-5b</i<-mediated signaling pathways. tomato spotted wilt virus <i<Sw-5b</i< NLR receptor plant innate immunity defense signaling Microbiology Qian Wu verfasserin aut Cong Tong verfasserin aut Hongyu Chen verfasserin aut Dan Miao verfasserin aut Xin Qian verfasserin aut Xiaohui Zhao verfasserin aut Lei Jiang verfasserin aut Xiaorong Tao verfasserin aut In Viruses MDPI AG, 2009 13(2021), 8, p 1447 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:13 year:2021 number:8, p 1447 https://doi.org/10.3390/v13081447 kostenfrei https://doaj.org/article/6121581466974b0fafe204fa3edad499 kostenfrei https://www.mdpi.com/1999-4915/13/8/1447 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 8, p 1447
allfieldsSound	10.3390/v13081447 doi (DE-627)DOAJ005193672 (DE-599)DOAJ6121581466974b0fafe204fa3edad499 DE-627 ger DE-627 rakwb eng QR1-502 Zhengqiang Chen verfasserin aut Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm elicitor has increased significantly, the process by which <i<Sw-5b</i< activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the <i<Sw-5b</i<-mediated signaling pathway. We found that chaperone SGT1 was required for <i<Sw-5b</i< function, but co-chaperone RAR1 was not. <i<Sw-5b</i<-mediated immune signaling was independent of both EDS1 and NDR1. Silencing <i<NPR1</i<, which is a central component in SA signaling, did not result in TSWV systemic infection in <i<Sw-5b</i<-transgenic <i<N. benthamiana</i< plants. Helper NLR NRCs (NLRs required for cell death) were required for <i<Sw-5b</i<-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the <i<Sw-5b</i< resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the <i<Sw-5b</i< signaling pathway. Silencing ADR1, NRG1, or both genes did not affect <i<Sw-5b</i<-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in <i<Sw-5b</i<-mediated signaling pathways. tomato spotted wilt virus <i<Sw-5b</i< NLR receptor plant innate immunity defense signaling Microbiology Qian Wu verfasserin aut Cong Tong verfasserin aut Hongyu Chen verfasserin aut Dan Miao verfasserin aut Xin Qian verfasserin aut Xiaohui Zhao verfasserin aut Lei Jiang verfasserin aut Xiaorong Tao verfasserin aut In Viruses MDPI AG, 2009 13(2021), 8, p 1447 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:13 year:2021 number:8, p 1447 https://doi.org/10.3390/v13081447 kostenfrei https://doaj.org/article/6121581466974b0fafe204fa3edad499 kostenfrei https://www.mdpi.com/1999-4915/13/8/1447 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 8, p 1447
language	English
source	In Viruses 13(2021), 8, p 1447 volume:13 year:2021 number:8, p 1447
sourceStr	In Viruses 13(2021), 8, p 1447 volume:13 year:2021 number:8, p 1447
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	tomato spotted wilt virus <i<Sw-5b</i< NLR receptor plant innate immunity defense signaling Microbiology
isfreeaccess_bool	true
container_title	Viruses
authorswithroles_txt_mv	Zhengqiang Chen @@aut@@ Qian Wu @@aut@@ Cong Tong @@aut@@ Hongyu Chen @@aut@@ Dan Miao @@aut@@ Xin Qian @@aut@@ Xiaohui Zhao @@aut@@ Lei Jiang @@aut@@ Xiaorong Tao @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	609775871
id	DOAJ005193672
language_de	englisch
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callnumber-first	Q - Science
author	Zhengqiang Chen
spellingShingle	Zhengqiang Chen misc QR1-502 misc tomato spotted wilt virus misc <i<Sw-5b</i< misc NLR receptor misc plant innate immunity misc defense signaling misc Microbiology Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus
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topic_title	QR1-502 Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus tomato spotted wilt virus <i<Sw-5b</i< NLR receptor plant innate immunity defense signaling
topic	misc QR1-502 misc tomato spotted wilt virus misc <i<Sw-5b</i< misc NLR receptor misc plant innate immunity misc defense signaling misc Microbiology
topic_unstemmed	misc QR1-502 misc tomato spotted wilt virus misc <i<Sw-5b</i< misc NLR receptor misc plant innate immunity misc defense signaling misc Microbiology
topic_browse	misc QR1-502 misc tomato spotted wilt virus misc <i<Sw-5b</i< misc NLR receptor misc plant innate immunity misc defense signaling misc Microbiology
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title	Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus
ctrlnum	(DE-627)DOAJ005193672 (DE-599)DOAJ6121581466974b0fafe204fa3edad499
title_full	Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus
author_sort	Zhengqiang Chen
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author_browse	Zhengqiang Chen Qian Wu Cong Tong Hongyu Chen Dan Miao Xin Qian Xiaohui Zhao Lei Jiang Xiaorong Tao
container_volume	13
class	QR1-502
format_se	Elektronische Aufsätze
author-letter	Zhengqiang Chen
doi_str_mv	10.3390/v13081447
author2-role	verfasserin
title_sort	characterization of the roles of sgt1/rar1, eds1/ndr1, npr1, and nrc/adr1/nrg1 in <em<sw-5b</em<-mediated resistance to tomato spotted wilt virus
callnumber	QR1-502
title_auth	Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus
abstract	The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm elicitor has increased significantly, the process by which <i<Sw-5b</i< activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the <i<Sw-5b</i<-mediated signaling pathway. We found that chaperone SGT1 was required for <i<Sw-5b</i< function, but co-chaperone RAR1 was not. <i<Sw-5b</i<-mediated immune signaling was independent of both EDS1 and NDR1. Silencing <i<NPR1</i<, which is a central component in SA signaling, did not result in TSWV systemic infection in <i<Sw-5b</i<-transgenic <i<N. benthamiana</i< plants. Helper NLR NRCs (NLRs required for cell death) were required for <i<Sw-5b</i<-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the <i<Sw-5b</i< resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the <i<Sw-5b</i< signaling pathway. Silencing ADR1, NRG1, or both genes did not affect <i<Sw-5b</i<-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in <i<Sw-5b</i<-mediated signaling pathways.
abstractGer	The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm elicitor has increased significantly, the process by which <i<Sw-5b</i< activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the <i<Sw-5b</i<-mediated signaling pathway. We found that chaperone SGT1 was required for <i<Sw-5b</i< function, but co-chaperone RAR1 was not. <i<Sw-5b</i<-mediated immune signaling was independent of both EDS1 and NDR1. Silencing <i<NPR1</i<, which is a central component in SA signaling, did not result in TSWV systemic infection in <i<Sw-5b</i<-transgenic <i<N. benthamiana</i< plants. Helper NLR NRCs (NLRs required for cell death) were required for <i<Sw-5b</i<-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the <i<Sw-5b</i< resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the <i<Sw-5b</i< signaling pathway. Silencing ADR1, NRG1, or both genes did not affect <i<Sw-5b</i<-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in <i<Sw-5b</i<-mediated signaling pathways.
abstract_unstemmed	The tomato <i<Sw-5b</i< gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how <i<Sw-5b</i< recognizes the viral NSm elicitor has increased significantly, the process by which <i<Sw-5b</i< activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the <i<Sw-5b</i<-mediated signaling pathway. We found that chaperone SGT1 was required for <i<Sw-5b</i< function, but co-chaperone RAR1 was not. <i<Sw-5b</i<-mediated immune signaling was independent of both EDS1 and NDR1. Silencing <i<NPR1</i<, which is a central component in SA signaling, did not result in TSWV systemic infection in <i<Sw-5b</i<-transgenic <i<N. benthamiana</i< plants. Helper NLR NRCs (NLRs required for cell death) were required for <i<Sw-5b</i<-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the <i<Sw-5b</i< resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the <i<Sw-5b</i< signaling pathway. Silencing ADR1, NRG1, or both genes did not affect <i<Sw-5b</i<-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in <i<Sw-5b</i<-mediated signaling pathways.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	8, p 1447
title_short	Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus
url	https://doi.org/10.3390/v13081447 https://doaj.org/article/6121581466974b0fafe204fa3edad499 https://www.mdpi.com/1999-4915/13/8/1447 https://doaj.org/toc/1999-4915
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up_date	2024-07-03T13:33:23.706Z
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Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in <em<Sw-5b</em<-Mediated Resistance to Tomato Spotted Wilt Virus

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