Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt)

A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherich...
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Autor*in:	Herlina Rante [verfasserIn] Risfah Yulianty [verfasserIn] Yayu Mulsiani Evary [verfasserIn] Elvira Hardiana [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	antibacterial activity endophytic fungi melochia umbellata mycelia

Übergeordnetes Werk:	In: Journal of Pure and Applied Microbiology - Journal of Pure and Applied Microbiology, 2019, 11(2017), 3, Seite 1313-1318
Übergeordnetes Werk:	volume:11 ; year:2017 ; number:3 ; pages:1313-1318

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.22207/JPAM.11.3.11

Katalog-ID:	DOAJ005206588

Internformat


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520			\|a A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigella dysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethyl acetate and methanol extracts were assessed for their antibacterial activity against the pathogenic bacteria and some of the isolates extract showed antibacterial activity with inhibition zone obtained were between 0 and 10.51 mm. From ethyl acetate extracts, We were obtained MUD5 94 mg with inhibition zone 8.08 mm against E. coli, 10.51 against S. dysentriae, 7.81 mm against P. aeruginosa, 9.00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8.61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7.54 mm against E. coli, and MUR6 96 mg with inhibition zone 7.44 mg against P. aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9.3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endophytic fungi could be promising source of bioactive compounds and can be used for further study.
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publishDate	2017
allfields	10.22207/JPAM.11.3.11 doi (DE-627)DOAJ005206588 (DE-599)DOAJ2137e5f5014d43589cc4011ebba5f725 DE-627 ger DE-627 rakwb eng QR1-502 Herlina Rante verfasserin aut Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt) 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigella dysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethyl acetate and methanol extracts were assessed for their antibacterial activity against the pathogenic bacteria and some of the isolates extract showed antibacterial activity with inhibition zone obtained were between 0 and 10.51 mm. From ethyl acetate extracts, We were obtained MUD5 94 mg with inhibition zone 8.08 mm against E. coli, 10.51 against S. dysentriae, 7.81 mm against P. aeruginosa, 9.00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8.61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7.54 mm against E. coli, and MUR6 96 mg with inhibition zone 7.44 mg against P. aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9.3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endophytic fungi could be promising source of bioactive compounds and can be used for further study. antibacterial activity endophytic fungi melochia umbellata mycelia Microbiology Risfah Yulianty verfasserin aut Yayu Mulsiani Evary verfasserin aut Elvira Hardiana verfasserin aut In Journal of Pure and Applied Microbiology Journal of Pure and Applied Microbiology, 2019 11(2017), 3, Seite 1313-1318 (DE-627)857236156 (DE-600)2853403-7 2581690X nnns volume:11 year:2017 number:3 pages:1313-1318 https://doi.org/10.22207/JPAM.11.3.11 kostenfrei https://doaj.org/article/2137e5f5014d43589cc4011ebba5f725 kostenfrei https://microbiologyjournal.org/isolation-and-antibacterial-activity-of-endophytic-fungi-frommelochiaumbellatahoutt/ kostenfrei https://doaj.org/toc/0973-7510 Journal toc kostenfrei https://doaj.org/toc/2581-690X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2017 3 1313-1318
spelling	10.22207/JPAM.11.3.11 doi (DE-627)DOAJ005206588 (DE-599)DOAJ2137e5f5014d43589cc4011ebba5f725 DE-627 ger DE-627 rakwb eng QR1-502 Herlina Rante verfasserin aut Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt) 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigella dysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethyl acetate and methanol extracts were assessed for their antibacterial activity against the pathogenic bacteria and some of the isolates extract showed antibacterial activity with inhibition zone obtained were between 0 and 10.51 mm. From ethyl acetate extracts, We were obtained MUD5 94 mg with inhibition zone 8.08 mm against E. coli, 10.51 against S. dysentriae, 7.81 mm against P. aeruginosa, 9.00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8.61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7.54 mm against E. coli, and MUR6 96 mg with inhibition zone 7.44 mg against P. aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9.3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endophytic fungi could be promising source of bioactive compounds and can be used for further study. antibacterial activity endophytic fungi melochia umbellata mycelia Microbiology Risfah Yulianty verfasserin aut Yayu Mulsiani Evary verfasserin aut Elvira Hardiana verfasserin aut In Journal of Pure and Applied Microbiology Journal of Pure and Applied Microbiology, 2019 11(2017), 3, Seite 1313-1318 (DE-627)857236156 (DE-600)2853403-7 2581690X nnns volume:11 year:2017 number:3 pages:1313-1318 https://doi.org/10.22207/JPAM.11.3.11 kostenfrei https://doaj.org/article/2137e5f5014d43589cc4011ebba5f725 kostenfrei https://microbiologyjournal.org/isolation-and-antibacterial-activity-of-endophytic-fungi-frommelochiaumbellatahoutt/ kostenfrei https://doaj.org/toc/0973-7510 Journal toc kostenfrei https://doaj.org/toc/2581-690X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2017 3 1313-1318
allfields_unstemmed	10.22207/JPAM.11.3.11 doi (DE-627)DOAJ005206588 (DE-599)DOAJ2137e5f5014d43589cc4011ebba5f725 DE-627 ger DE-627 rakwb eng QR1-502 Herlina Rante verfasserin aut Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt) 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigella dysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethyl acetate and methanol extracts were assessed for their antibacterial activity against the pathogenic bacteria and some of the isolates extract showed antibacterial activity with inhibition zone obtained were between 0 and 10.51 mm. From ethyl acetate extracts, We were obtained MUD5 94 mg with inhibition zone 8.08 mm against E. coli, 10.51 against S. dysentriae, 7.81 mm against P. aeruginosa, 9.00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8.61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7.54 mm against E. coli, and MUR6 96 mg with inhibition zone 7.44 mg against P. aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9.3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endophytic fungi could be promising source of bioactive compounds and can be used for further study. antibacterial activity endophytic fungi melochia umbellata mycelia Microbiology Risfah Yulianty verfasserin aut Yayu Mulsiani Evary verfasserin aut Elvira Hardiana verfasserin aut In Journal of Pure and Applied Microbiology Journal of Pure and Applied Microbiology, 2019 11(2017), 3, Seite 1313-1318 (DE-627)857236156 (DE-600)2853403-7 2581690X nnns volume:11 year:2017 number:3 pages:1313-1318 https://doi.org/10.22207/JPAM.11.3.11 kostenfrei https://doaj.org/article/2137e5f5014d43589cc4011ebba5f725 kostenfrei https://microbiologyjournal.org/isolation-and-antibacterial-activity-of-endophytic-fungi-frommelochiaumbellatahoutt/ kostenfrei https://doaj.org/toc/0973-7510 Journal toc kostenfrei https://doaj.org/toc/2581-690X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2017 3 1313-1318
allfieldsGer	10.22207/JPAM.11.3.11 doi (DE-627)DOAJ005206588 (DE-599)DOAJ2137e5f5014d43589cc4011ebba5f725 DE-627 ger DE-627 rakwb eng QR1-502 Herlina Rante verfasserin aut Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt) 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigella dysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethyl acetate and methanol extracts were assessed for their antibacterial activity against the pathogenic bacteria and some of the isolates extract showed antibacterial activity with inhibition zone obtained were between 0 and 10.51 mm. From ethyl acetate extracts, We were obtained MUD5 94 mg with inhibition zone 8.08 mm against E. coli, 10.51 against S. dysentriae, 7.81 mm against P. aeruginosa, 9.00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8.61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7.54 mm against E. coli, and MUR6 96 mg with inhibition zone 7.44 mg against P. aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9.3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endophytic fungi could be promising source of bioactive compounds and can be used for further study. antibacterial activity endophytic fungi melochia umbellata mycelia Microbiology Risfah Yulianty verfasserin aut Yayu Mulsiani Evary verfasserin aut Elvira Hardiana verfasserin aut In Journal of Pure and Applied Microbiology Journal of Pure and Applied Microbiology, 2019 11(2017), 3, Seite 1313-1318 (DE-627)857236156 (DE-600)2853403-7 2581690X nnns volume:11 year:2017 number:3 pages:1313-1318 https://doi.org/10.22207/JPAM.11.3.11 kostenfrei https://doaj.org/article/2137e5f5014d43589cc4011ebba5f725 kostenfrei https://microbiologyjournal.org/isolation-and-antibacterial-activity-of-endophytic-fungi-frommelochiaumbellatahoutt/ kostenfrei https://doaj.org/toc/0973-7510 Journal toc kostenfrei https://doaj.org/toc/2581-690X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2017 3 1313-1318
allfieldsSound	10.22207/JPAM.11.3.11 doi (DE-627)DOAJ005206588 (DE-599)DOAJ2137e5f5014d43589cc4011ebba5f725 DE-627 ger DE-627 rakwb eng QR1-502 Herlina Rante verfasserin aut Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt) 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigella dysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethyl acetate and methanol extracts were assessed for their antibacterial activity against the pathogenic bacteria and some of the isolates extract showed antibacterial activity with inhibition zone obtained were between 0 and 10.51 mm. From ethyl acetate extracts, We were obtained MUD5 94 mg with inhibition zone 8.08 mm against E. coli, 10.51 against S. dysentriae, 7.81 mm against P. aeruginosa, 9.00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8.61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7.54 mm against E. coli, and MUR6 96 mg with inhibition zone 7.44 mg against P. aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9.3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endophytic fungi could be promising source of bioactive compounds and can be used for further study. antibacterial activity endophytic fungi melochia umbellata mycelia Microbiology Risfah Yulianty verfasserin aut Yayu Mulsiani Evary verfasserin aut Elvira Hardiana verfasserin aut In Journal of Pure and Applied Microbiology Journal of Pure and Applied Microbiology, 2019 11(2017), 3, Seite 1313-1318 (DE-627)857236156 (DE-600)2853403-7 2581690X nnns volume:11 year:2017 number:3 pages:1313-1318 https://doi.org/10.22207/JPAM.11.3.11 kostenfrei https://doaj.org/article/2137e5f5014d43589cc4011ebba5f725 kostenfrei https://microbiologyjournal.org/isolation-and-antibacterial-activity-of-endophytic-fungi-frommelochiaumbellatahoutt/ kostenfrei https://doaj.org/toc/0973-7510 Journal toc kostenfrei https://doaj.org/toc/2581-690X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2017 3 1313-1318
language	English
source	In Journal of Pure and Applied Microbiology 11(2017), 3, Seite 1313-1318 volume:11 year:2017 number:3 pages:1313-1318
sourceStr	In Journal of Pure and Applied Microbiology 11(2017), 3, Seite 1313-1318 volume:11 year:2017 number:3 pages:1313-1318
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	antibacterial activity endophytic fungi melochia umbellata mycelia Microbiology
isfreeaccess_bool	true
container_title	Journal of Pure and Applied Microbiology
authorswithroles_txt_mv	Herlina Rante @@aut@@ Risfah Yulianty @@aut@@ Yayu Mulsiani Evary @@aut@@ Elvira Hardiana @@aut@@
publishDateDaySort_date	2017-01-01T00:00:00Z
hierarchy_top_id	857236156
id	DOAJ005206588
language_de	englisch
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callnumber-first	Q - Science
author	Herlina Rante
spellingShingle	Herlina Rante misc QR1-502 misc antibacterial activity misc endophytic fungi misc melochia umbellata misc mycelia misc Microbiology Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt)
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topic_title	QR1-502 Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt) antibacterial activity endophytic fungi melochia umbellata mycelia
topic	misc QR1-502 misc antibacterial activity misc endophytic fungi misc melochia umbellata misc mycelia misc Microbiology
topic_unstemmed	misc QR1-502 misc antibacterial activity misc endophytic fungi misc melochia umbellata misc mycelia misc Microbiology
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title	Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt)
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title_full	Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt)
author_sort	Herlina Rante
journal	Journal of Pure and Applied Microbiology
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author_browse	Herlina Rante Risfah Yulianty Yayu Mulsiani Evary Elvira Hardiana
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author-letter	Herlina Rante
doi_str_mv	10.22207/JPAM.11.3.11
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title_sort	isolation and antibacterial activity of endophytic fungi from melochia umbellata (houtt)
callnumber	QR1-502
title_auth	Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt)
abstract	A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigella dysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethyl acetate and methanol extracts were assessed for their antibacterial activity against the pathogenic bacteria and some of the isolates extract showed antibacterial activity with inhibition zone obtained were between 0 and 10.51 mm. From ethyl acetate extracts, We were obtained MUD5 94 mg with inhibition zone 8.08 mm against E. coli, 10.51 against S. dysentriae, 7.81 mm against P. aeruginosa, 9.00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8.61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7.54 mm against E. coli, and MUR6 96 mg with inhibition zone 7.44 mg against P. aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9.3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endophytic fungi could be promising source of bioactive compounds and can be used for further study.
abstractGer	A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigella dysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethyl acetate and methanol extracts were assessed for their antibacterial activity against the pathogenic bacteria and some of the isolates extract showed antibacterial activity with inhibition zone obtained were between 0 and 10.51 mm. From ethyl acetate extracts, We were obtained MUD5 94 mg with inhibition zone 8.08 mm against E. coli, 10.51 against S. dysentriae, 7.81 mm against P. aeruginosa, 9.00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8.61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7.54 mm against E. coli, and MUR6 96 mg with inhibition zone 7.44 mg against P. aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9.3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endophytic fungi could be promising source of bioactive compounds and can be used for further study.
abstract_unstemmed	A study observing endophytic fungi from medicinal plant Melochia umbellata and their antibacterial potencies has been done. Twelve species of endophytic fungi were successfully isolated from Melochia umbellata leaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigella dysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethyl acetate and methanol extracts were assessed for their antibacterial activity against the pathogenic bacteria and some of the isolates extract showed antibacterial activity with inhibition zone obtained were between 0 and 10.51 mm. From ethyl acetate extracts, We were obtained MUD5 94 mg with inhibition zone 8.08 mm against E. coli, 10.51 against S. dysentriae, 7.81 mm against P. aeruginosa, 9.00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8.61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7.54 mm against E. coli, and MUR6 96 mg with inhibition zone 7.44 mg against P. aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9.3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endophytic fungi could be promising source of bioactive compounds and can be used for further study.
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container_issue	3
title_short	Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt)
url	https://doi.org/10.22207/JPAM.11.3.11 https://doaj.org/article/2137e5f5014d43589cc4011ebba5f725 https://microbiologyjournal.org/isolation-and-antibacterial-activity-of-endophytic-fungi-frommelochiaumbellatahoutt/ https://doaj.org/toc/0973-7510 https://doaj.org/toc/2581-690X
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up_date	2024-07-03T13:38:04.900Z
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Isolation and Antibacterial Activity of Endophytic Fungi from Melochia umbellata (Houtt)

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