Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage

Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific f...
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Autor*in:	Jin Su Choi [verfasserIn] Seul Gi Yang [verfasserIn] Jeong Young Song [verfasserIn] Hong Gi Kim [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Koreanisch

Erschienen:	2014

Schlagwörter:	Clubroot pathogen Detection Plasmodiophora brassicae Quantitative real-time PCR

Übergeordnetes Werk:	In: Research in Plant Disease - Hanrimwon Publishing Company, 2016, 20(2014), 1, Seite 21-24
Übergeordnetes Werk:	volume:20 ; year:2014 ; number:1 ; pages:21-24

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ005294312

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520			\|a Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific primer set could be useful for rapid and accurate detection of P. brassicae.
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language	English Korean
source	In Research in Plant Disease 20(2014), 1, Seite 21-24 volume:20 year:2014 number:1 pages:21-24
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topic_facet	Clubroot pathogen Detection Plasmodiophora brassicae Quantitative real-time PCR Agriculture (General)
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authorswithroles_txt_mv	Jin Su Choi @@aut@@ Seul Gi Yang @@aut@@ Jeong Young Song @@aut@@ Hong Gi Kim @@aut@@
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callnumber-first	S - Agriculture
author	Jin Su Choi
spellingShingle	Jin Su Choi misc S1-972 misc Clubroot pathogen misc Detection misc Plasmodiophora brassicae misc Quantitative real-time PCR misc Agriculture (General) Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage
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topic_title	S1-972 Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage Clubroot pathogen Detection Plasmodiophora brassicae Quantitative real-time PCR
topic	misc S1-972 misc Clubroot pathogen misc Detection misc Plasmodiophora brassicae misc Quantitative real-time PCR misc Agriculture (General)
topic_unstemmed	misc S1-972 misc Clubroot pathogen misc Detection misc Plasmodiophora brassicae misc Quantitative real-time PCR misc Agriculture (General)
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title	Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage
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title_sort	development of species-specific primers for plasmodiophora brassicae, clubroot pathogen of kimchi cabbage
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title_auth	Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage
abstract	Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific primer set could be useful for rapid and accurate detection of P. brassicae.
abstractGer	Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific primer set could be useful for rapid and accurate detection of P. brassicae.
abstract_unstemmed	Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific primer set could be useful for rapid and accurate detection of P. brassicae.
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title_short	Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage
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Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage

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Zugang & Verfügbarkeit

Vorhandene Bände

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