Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro

Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neuro...
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Autor*in:	Keqin Li [verfasserIn] Shuai Shao [verfasserIn] Tongjie Ji [verfasserIn] Min Liu [verfasserIn] Lufeng Wang [verfasserIn] Ying Pang [verfasserIn] Mu Chen [verfasserIn] Siyi Xu [verfasserIn] Kuiming Zhang [verfasserIn] Qi Wang [verfasserIn] Zhongwei Zhuang [verfasserIn] Liang Wei [verfasserIn] Yanfei Zhang [verfasserIn] Yanlin Chen [verfasserIn] Yang Wang [verfasserIn] Jing Zhang [verfasserIn] Kui Chen [verfasserIn] Hao Lian [verfasserIn] Chunlong Zhong [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	capicua transcriptional repressor dendritic growth spine growth Ets factors

Übergeordnetes Werk:	In: Frontiers in Neuroanatomy - Frontiers Media S.A., 2008, 15(2021)
Übergeordnetes Werk:	volume:15 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fnana.2021.669310

Katalog-ID:	DOAJ005361532

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520			\|a Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that Cic has a function in dendrite growth. Loss-of-function and gain-of function assays indicated that Cic plays a central role in the inhibition of dendritic morphogenesis and dendritic spines in vitro. Further studies showed that overexpression of Cic reduced the expression of Ets in HT22 cells, while in vitro knockdown of Cic in hippocampal neurons significantly elevated the expression of Ets. These results suggest that Cic may negatively control dendrite growth through Ets, which was confirmed by ShRNA knockdown of either Etv4 or Etv5 abolishing the phenotype of Cic knockdown in cultured neurons. Taken together, our results suggest that Cic inhibits dendritic morphogenesis and the growth of dendritic spines through Ets.
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callnumber-first	R - Medicine
author	Keqin Li
spellingShingle	Keqin Li misc RC321-571 misc QM1-695 misc capicua misc transcriptional repressor misc dendritic growth misc spine growth misc Ets factors misc Neurosciences. Biological psychiatry. Neuropsychiatry misc Human anatomy Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro
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topic_title	RC321-571 QM1-695 Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro capicua transcriptional repressor dendritic growth spine growth Ets factors
topic	misc RC321-571 misc QM1-695 misc capicua misc transcriptional repressor misc dendritic growth misc spine growth misc Ets factors misc Neurosciences. Biological psychiatry. Neuropsychiatry misc Human anatomy
topic_unstemmed	misc RC321-571 misc QM1-695 misc capicua misc transcriptional repressor misc dendritic growth misc spine growth misc Ets factors misc Neurosciences. Biological psychiatry. Neuropsychiatry misc Human anatomy
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title	Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro
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title_full	Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro
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author_browse	Keqin Li Shuai Shao Tongjie Ji Min Liu Lufeng Wang Ying Pang Mu Chen Siyi Xu Kuiming Zhang Qi Wang Zhongwei Zhuang Liang Wei Yanfei Zhang Yanlin Chen Yang Wang Jing Zhang Kui Chen Hao Lian Chunlong Zhong
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title_sort	capicua regulates dendritic morphogenesis through ets in hippocampal neurons in vitro
callnumber	RC321-571
title_auth	Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro
abstract	Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that Cic has a function in dendrite growth. Loss-of-function and gain-of function assays indicated that Cic plays a central role in the inhibition of dendritic morphogenesis and dendritic spines in vitro. Further studies showed that overexpression of Cic reduced the expression of Ets in HT22 cells, while in vitro knockdown of Cic in hippocampal neurons significantly elevated the expression of Ets. These results suggest that Cic may negatively control dendrite growth through Ets, which was confirmed by ShRNA knockdown of either Etv4 or Etv5 abolishing the phenotype of Cic knockdown in cultured neurons. Taken together, our results suggest that Cic inhibits dendritic morphogenesis and the growth of dendritic spines through Ets.
abstractGer	Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that Cic has a function in dendrite growth. Loss-of-function and gain-of function assays indicated that Cic plays a central role in the inhibition of dendritic morphogenesis and dendritic spines in vitro. Further studies showed that overexpression of Cic reduced the expression of Ets in HT22 cells, while in vitro knockdown of Cic in hippocampal neurons significantly elevated the expression of Ets. These results suggest that Cic may negatively control dendrite growth through Ets, which was confirmed by ShRNA knockdown of either Etv4 or Etv5 abolishing the phenotype of Cic knockdown in cultured neurons. Taken together, our results suggest that Cic inhibits dendritic morphogenesis and the growth of dendritic spines through Ets.
abstract_unstemmed	Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that Cic has a function in dendrite growth. Loss-of-function and gain-of function assays indicated that Cic plays a central role in the inhibition of dendritic morphogenesis and dendritic spines in vitro. Further studies showed that overexpression of Cic reduced the expression of Ets in HT22 cells, while in vitro knockdown of Cic in hippocampal neurons significantly elevated the expression of Ets. These results suggest that Cic may negatively control dendrite growth through Ets, which was confirmed by ShRNA knockdown of either Etv4 or Etv5 abolishing the phenotype of Cic knockdown in cultured neurons. Taken together, our results suggest that Cic inhibits dendritic morphogenesis and the growth of dendritic spines through Ets.
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title_short	Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro
url	https://doi.org/10.3389/fnana.2021.669310 https://doaj.org/article/5918075d2328459e9ed8f90301d4fe9e https://www.frontiersin.org/articles/10.3389/fnana.2021.669310/full https://doaj.org/toc/1662-5129
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author2	Shuai Shao Tongjie Ji Min Liu Lufeng Wang Ying Pang Mu Chen Siyi Xu Kuiming Zhang Qi Wang Zhongwei Zhuang Liang Wei Yanfei Zhang Yanlin Chen Yang Wang Jing Zhang Kui Chen Hao Lian Chunlong Zhong
author2Str	Shuai Shao Tongjie Ji Min Liu Lufeng Wang Ying Pang Mu Chen Siyi Xu Kuiming Zhang Qi Wang Zhongwei Zhuang Liang Wei Yanfei Zhang Yanlin Chen Yang Wang Jing Zhang Kui Chen Hao Lian Chunlong Zhong
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doi_str	10.3389/fnana.2021.669310
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up_date	2024-07-03T14:33:20.847Z
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However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that Cic has a function in dendrite growth. Loss-of-function and gain-of function assays indicated that Cic plays a central role in the inhibition of dendritic morphogenesis and dendritic spines in vitro. Further studies showed that overexpression of Cic reduced the expression of Ets in HT22 cells, while in vitro knockdown of Cic in hippocampal neurons significantly elevated the expression of Ets. These results suggest that Cic may negatively control dendrite growth through Ets, which was confirmed by ShRNA knockdown of either Etv4 or Etv5 abolishing the phenotype of Cic knockdown in cultured neurons. 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Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro

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