Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests

Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem...
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Gespeichert in:

Autor*in:	Gloria Melzi [verfasserIn] Corrado L. Galli [verfasserIn] Paola Ciliutti [verfasserIn] Cristina Marabottini [verfasserIn] Marina Marinovich [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Herbal medicine Herbal food supplements Botanical extract Genotoxicity Ames test in vitro micronucleus test

Übergeordnetes Werk:	In: Toxicology Reports - Elsevier, 2016, 9(2022), Seite 1574-1579
Übergeordnetes Werk:	volume:9 ; year:2022 ; pages:1574-1579

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.toxrep.2022.07.017

Katalog-ID:	DOAJ005590108

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520			\|a Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. Under the experimental conditions used, the rhubarb rhizome extract showed no genotoxic activity.
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700	0		\|a Marina Marinovich \|e verfasserin \|4 aut
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allfields	10.1016/j.toxrep.2022.07.017 doi (DE-627)DOAJ005590108 (DE-599)DOAJ63935ad260664ec6aad76608f1686024 DE-627 ger DE-627 rakwb eng RA1190-1270 Gloria Melzi verfasserin aut Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. Under the experimental conditions used, the rhubarb rhizome extract showed no genotoxic activity. Herbal medicine Herbal food supplements Botanical extract Genotoxicity Ames test in vitro micronucleus test Toxicology. Poisons Corrado L. Galli verfasserin aut Paola Ciliutti verfasserin aut Cristina Marabottini verfasserin aut Marina Marinovich verfasserin aut In Toxicology Reports Elsevier, 2016 9(2022), Seite 1574-1579 (DE-627)815397534 (DE-600)2805786-7 22147500 nnns volume:9 year:2022 pages:1574-1579 https://doi.org/10.1016/j.toxrep.2022.07.017 kostenfrei https://doaj.org/article/63935ad260664ec6aad76608f1686024 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214750022001718 kostenfrei https://doaj.org/toc/2214-7500 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 9 2022 1574-1579
spelling	10.1016/j.toxrep.2022.07.017 doi (DE-627)DOAJ005590108 (DE-599)DOAJ63935ad260664ec6aad76608f1686024 DE-627 ger DE-627 rakwb eng RA1190-1270 Gloria Melzi verfasserin aut Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. Under the experimental conditions used, the rhubarb rhizome extract showed no genotoxic activity. Herbal medicine Herbal food supplements Botanical extract Genotoxicity Ames test in vitro micronucleus test Toxicology. Poisons Corrado L. Galli verfasserin aut Paola Ciliutti verfasserin aut Cristina Marabottini verfasserin aut Marina Marinovich verfasserin aut In Toxicology Reports Elsevier, 2016 9(2022), Seite 1574-1579 (DE-627)815397534 (DE-600)2805786-7 22147500 nnns volume:9 year:2022 pages:1574-1579 https://doi.org/10.1016/j.toxrep.2022.07.017 kostenfrei https://doaj.org/article/63935ad260664ec6aad76608f1686024 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214750022001718 kostenfrei https://doaj.org/toc/2214-7500 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 9 2022 1574-1579
allfields_unstemmed	10.1016/j.toxrep.2022.07.017 doi (DE-627)DOAJ005590108 (DE-599)DOAJ63935ad260664ec6aad76608f1686024 DE-627 ger DE-627 rakwb eng RA1190-1270 Gloria Melzi verfasserin aut Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. Under the experimental conditions used, the rhubarb rhizome extract showed no genotoxic activity. Herbal medicine Herbal food supplements Botanical extract Genotoxicity Ames test in vitro micronucleus test Toxicology. Poisons Corrado L. Galli verfasserin aut Paola Ciliutti verfasserin aut Cristina Marabottini verfasserin aut Marina Marinovich verfasserin aut In Toxicology Reports Elsevier, 2016 9(2022), Seite 1574-1579 (DE-627)815397534 (DE-600)2805786-7 22147500 nnns volume:9 year:2022 pages:1574-1579 https://doi.org/10.1016/j.toxrep.2022.07.017 kostenfrei https://doaj.org/article/63935ad260664ec6aad76608f1686024 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214750022001718 kostenfrei https://doaj.org/toc/2214-7500 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 9 2022 1574-1579
allfieldsGer	10.1016/j.toxrep.2022.07.017 doi (DE-627)DOAJ005590108 (DE-599)DOAJ63935ad260664ec6aad76608f1686024 DE-627 ger DE-627 rakwb eng RA1190-1270 Gloria Melzi verfasserin aut Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. Under the experimental conditions used, the rhubarb rhizome extract showed no genotoxic activity. Herbal medicine Herbal food supplements Botanical extract Genotoxicity Ames test in vitro micronucleus test Toxicology. Poisons Corrado L. Galli verfasserin aut Paola Ciliutti verfasserin aut Cristina Marabottini verfasserin aut Marina Marinovich verfasserin aut In Toxicology Reports Elsevier, 2016 9(2022), Seite 1574-1579 (DE-627)815397534 (DE-600)2805786-7 22147500 nnns volume:9 year:2022 pages:1574-1579 https://doi.org/10.1016/j.toxrep.2022.07.017 kostenfrei https://doaj.org/article/63935ad260664ec6aad76608f1686024 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214750022001718 kostenfrei https://doaj.org/toc/2214-7500 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 9 2022 1574-1579
allfieldsSound	10.1016/j.toxrep.2022.07.017 doi (DE-627)DOAJ005590108 (DE-599)DOAJ63935ad260664ec6aad76608f1686024 DE-627 ger DE-627 rakwb eng RA1190-1270 Gloria Melzi verfasserin aut Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. Under the experimental conditions used, the rhubarb rhizome extract showed no genotoxic activity. Herbal medicine Herbal food supplements Botanical extract Genotoxicity Ames test in vitro micronucleus test Toxicology. Poisons Corrado L. Galli verfasserin aut Paola Ciliutti verfasserin aut Cristina Marabottini verfasserin aut Marina Marinovich verfasserin aut In Toxicology Reports Elsevier, 2016 9(2022), Seite 1574-1579 (DE-627)815397534 (DE-600)2805786-7 22147500 nnns volume:9 year:2022 pages:1574-1579 https://doi.org/10.1016/j.toxrep.2022.07.017 kostenfrei https://doaj.org/article/63935ad260664ec6aad76608f1686024 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214750022001718 kostenfrei https://doaj.org/toc/2214-7500 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 9 2022 1574-1579
language	English
source	In Toxicology Reports 9(2022), Seite 1574-1579 volume:9 year:2022 pages:1574-1579
sourceStr	In Toxicology Reports 9(2022), Seite 1574-1579 volume:9 year:2022 pages:1574-1579
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Herbal medicine Herbal food supplements Botanical extract Genotoxicity Ames test in vitro micronucleus test Toxicology. Poisons
isfreeaccess_bool	true
container_title	Toxicology Reports
authorswithroles_txt_mv	Gloria Melzi @@aut@@ Corrado L. Galli @@aut@@ Paola Ciliutti @@aut@@ Cristina Marabottini @@aut@@ Marina Marinovich @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	815397534
id	DOAJ005590108
language_de	englisch
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callnumber-first	R - Medicine
author	Gloria Melzi
spellingShingle	Gloria Melzi misc RA1190-1270 misc Herbal medicine misc Herbal food supplements misc Botanical extract misc Genotoxicity misc Ames test misc in vitro micronucleus test misc Toxicology. Poisons Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests
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topic_title	RA1190-1270 Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests Herbal medicine Herbal food supplements Botanical extract Genotoxicity Ames test in vitro micronucleus test
topic	misc RA1190-1270 misc Herbal medicine misc Herbal food supplements misc Botanical extract misc Genotoxicity misc Ames test misc in vitro micronucleus test misc Toxicology. Poisons
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title	Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests
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title_full	Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests
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title_sort	lack of genotoxicity of rhubarb (rhizome) in the ames and micronucleus in vitro tests
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title_auth	Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests
abstract	Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. Under the experimental conditions used, the rhubarb rhizome extract showed no genotoxic activity.
abstractGer	Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. Under the experimental conditions used, the rhubarb rhizome extract showed no genotoxic activity.
abstract_unstemmed	Hydroxyanthracene derivatives are widely distributed in the plant kingdom, mainly in botanicals such as the Hypericum, Rheum, Rhamnus and Aloe genera. For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. Under the experimental conditions used, the rhubarb rhizome extract showed no genotoxic activity.
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title_short	Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests
url	https://doi.org/10.1016/j.toxrep.2022.07.017 https://doaj.org/article/63935ad260664ec6aad76608f1686024 http://www.sciencedirect.com/science/article/pii/S2214750022001718 https://doaj.org/toc/2214-7500
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up_date	2024-07-03T15:56:20.919Z
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For centuries, plants containing hydroxyanthracene derivatives have been used as herbal remedies, mainly as laxatives. The root and underground stem (rhizome) are used to make medicine, primarily for digestive complaints including constipation, diarrhoea, heartburn, stomach pain, gastrointestinal bleeding, and preparation for certain gastrointestinal diagnostic procedures. The use of hydroxyanthracene-containing botanicals has raised the attention of European Food Safety Authority (EFSA) for the potential genotoxicity activity, that in 2018 concluded “[.] and that there is a safety concern for extracts containing hydroxyanthracene derivatives although uncertainty persists”. No genotoxic activity has been reported with other constituents such as rhein, physcion and chrysophanol. In the present study, Rhubarb ethanolic extract of ground rhubarb rhizome (hydroxyanthracene total content 1.39 %) was tested in the Ames Assay in Salmonella typhimurium and Escherichia coli, up to 5000 µg/plate and up to 5000 µg/mL in human lymphocytes Micronucleus Test (OECD 471 and 487 respectively) in vitro mutagenic and genotoxic effects. 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Lack of genotoxicity of rhubarb (rhizome) in the Ames and micronucleus in vitro tests

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