Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells

Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth an...
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Autor*in:	Valentina Coccè [verfasserIn] Silvia La Monica [verfasserIn] Mara Bonelli [verfasserIn] Giulio Alessandri [verfasserIn] Roberta Alfieri [verfasserIn] Costanza Annamaria Lagrasta [verfasserIn] Denise Madeddu [verfasserIn] Caterina Frati [verfasserIn] Lisa Flammini [verfasserIn] Daniela Lisini [verfasserIn] Angela Marcianti [verfasserIn] Eugenio Parati [verfasserIn] Francesca Paino [verfasserIn] Aldo Giannì [verfasserIn] Giampietro Farronato [verfasserIn] Angela Falco [verfasserIn] Lorenzo Spaggiari [verfasserIn] Francesco Petrella [verfasserIn] Augusto Pessina [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	mesenchymal stromal cells mesothelioma malignant pleural mesothelioma (MPM) cell therapy

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 10(2021), 6, p 1427
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:6, p 1427

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells10061427

Katalog-ID:	DOAJ005921058

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allfields	10.3390/cells10061427 doi (DE-627)DOAJ005921058 (DE-599)DOAJ5043b2f15724475d949474c94b0dbd6f DE-627 ger DE-627 rakwb eng QH573-671 Valentina Coccè verfasserin aut Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM. Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice. Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment. Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy. mesenchymal stromal cells mesothelioma malignant pleural mesothelioma (MPM) cell therapy Cytology Silvia La Monica verfasserin aut Mara Bonelli verfasserin aut Giulio Alessandri verfasserin aut Roberta Alfieri verfasserin aut Costanza Annamaria Lagrasta verfasserin aut Denise Madeddu verfasserin aut Caterina Frati verfasserin aut Lisa Flammini verfasserin aut Daniela Lisini verfasserin aut Angela Marcianti verfasserin aut Eugenio Parati verfasserin aut Francesca Paino verfasserin aut Aldo Giannì verfasserin aut Giampietro Farronato verfasserin aut Angela Falco verfasserin aut Lorenzo Spaggiari verfasserin aut Francesco Petrella verfasserin aut Augusto Pessina verfasserin aut In Cells MDPI AG, 2012 10(2021), 6, p 1427 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:6, p 1427 https://doi.org/10.3390/cells10061427 kostenfrei https://doaj.org/article/5043b2f15724475d949474c94b0dbd6f kostenfrei https://www.mdpi.com/2073-4409/10/6/1427 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1427
spelling	10.3390/cells10061427 doi (DE-627)DOAJ005921058 (DE-599)DOAJ5043b2f15724475d949474c94b0dbd6f DE-627 ger DE-627 rakwb eng QH573-671 Valentina Coccè verfasserin aut Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM. Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice. Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment. Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy. mesenchymal stromal cells mesothelioma malignant pleural mesothelioma (MPM) cell therapy Cytology Silvia La Monica verfasserin aut Mara Bonelli verfasserin aut Giulio Alessandri verfasserin aut Roberta Alfieri verfasserin aut Costanza Annamaria Lagrasta verfasserin aut Denise Madeddu verfasserin aut Caterina Frati verfasserin aut Lisa Flammini verfasserin aut Daniela Lisini verfasserin aut Angela Marcianti verfasserin aut Eugenio Parati verfasserin aut Francesca Paino verfasserin aut Aldo Giannì verfasserin aut Giampietro Farronato verfasserin aut Angela Falco verfasserin aut Lorenzo Spaggiari verfasserin aut Francesco Petrella verfasserin aut Augusto Pessina verfasserin aut In Cells MDPI AG, 2012 10(2021), 6, p 1427 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:6, p 1427 https://doi.org/10.3390/cells10061427 kostenfrei https://doaj.org/article/5043b2f15724475d949474c94b0dbd6f kostenfrei https://www.mdpi.com/2073-4409/10/6/1427 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1427
allfields_unstemmed	10.3390/cells10061427 doi (DE-627)DOAJ005921058 (DE-599)DOAJ5043b2f15724475d949474c94b0dbd6f DE-627 ger DE-627 rakwb eng QH573-671 Valentina Coccè verfasserin aut Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM. Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice. Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment. Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy. mesenchymal stromal cells mesothelioma malignant pleural mesothelioma (MPM) cell therapy Cytology Silvia La Monica verfasserin aut Mara Bonelli verfasserin aut Giulio Alessandri verfasserin aut Roberta Alfieri verfasserin aut Costanza Annamaria Lagrasta verfasserin aut Denise Madeddu verfasserin aut Caterina Frati verfasserin aut Lisa Flammini verfasserin aut Daniela Lisini verfasserin aut Angela Marcianti verfasserin aut Eugenio Parati verfasserin aut Francesca Paino verfasserin aut Aldo Giannì verfasserin aut Giampietro Farronato verfasserin aut Angela Falco verfasserin aut Lorenzo Spaggiari verfasserin aut Francesco Petrella verfasserin aut Augusto Pessina verfasserin aut In Cells MDPI AG, 2012 10(2021), 6, p 1427 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:6, p 1427 https://doi.org/10.3390/cells10061427 kostenfrei https://doaj.org/article/5043b2f15724475d949474c94b0dbd6f kostenfrei https://www.mdpi.com/2073-4409/10/6/1427 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1427
allfieldsGer	10.3390/cells10061427 doi (DE-627)DOAJ005921058 (DE-599)DOAJ5043b2f15724475d949474c94b0dbd6f DE-627 ger DE-627 rakwb eng QH573-671 Valentina Coccè verfasserin aut Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM. Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice. Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment. Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy. mesenchymal stromal cells mesothelioma malignant pleural mesothelioma (MPM) cell therapy Cytology Silvia La Monica verfasserin aut Mara Bonelli verfasserin aut Giulio Alessandri verfasserin aut Roberta Alfieri verfasserin aut Costanza Annamaria Lagrasta verfasserin aut Denise Madeddu verfasserin aut Caterina Frati verfasserin aut Lisa Flammini verfasserin aut Daniela Lisini verfasserin aut Angela Marcianti verfasserin aut Eugenio Parati verfasserin aut Francesca Paino verfasserin aut Aldo Giannì verfasserin aut Giampietro Farronato verfasserin aut Angela Falco verfasserin aut Lorenzo Spaggiari verfasserin aut Francesco Petrella verfasserin aut Augusto Pessina verfasserin aut In Cells MDPI AG, 2012 10(2021), 6, p 1427 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:6, p 1427 https://doi.org/10.3390/cells10061427 kostenfrei https://doaj.org/article/5043b2f15724475d949474c94b0dbd6f kostenfrei https://www.mdpi.com/2073-4409/10/6/1427 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1427
allfieldsSound	10.3390/cells10061427 doi (DE-627)DOAJ005921058 (DE-599)DOAJ5043b2f15724475d949474c94b0dbd6f DE-627 ger DE-627 rakwb eng QH573-671 Valentina Coccè verfasserin aut Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM. Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice. Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment. Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy. mesenchymal stromal cells mesothelioma malignant pleural mesothelioma (MPM) cell therapy Cytology Silvia La Monica verfasserin aut Mara Bonelli verfasserin aut Giulio Alessandri verfasserin aut Roberta Alfieri verfasserin aut Costanza Annamaria Lagrasta verfasserin aut Denise Madeddu verfasserin aut Caterina Frati verfasserin aut Lisa Flammini verfasserin aut Daniela Lisini verfasserin aut Angela Marcianti verfasserin aut Eugenio Parati verfasserin aut Francesca Paino verfasserin aut Aldo Giannì verfasserin aut Giampietro Farronato verfasserin aut Angela Falco verfasserin aut Lorenzo Spaggiari verfasserin aut Francesco Petrella verfasserin aut Augusto Pessina verfasserin aut In Cells MDPI AG, 2012 10(2021), 6, p 1427 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:6, p 1427 https://doi.org/10.3390/cells10061427 kostenfrei https://doaj.org/article/5043b2f15724475d949474c94b0dbd6f kostenfrei https://www.mdpi.com/2073-4409/10/6/1427 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1427
language	English
source	In Cells 10(2021), 6, p 1427 volume:10 year:2021 number:6, p 1427
sourceStr	In Cells 10(2021), 6, p 1427 volume:10 year:2021 number:6, p 1427
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	mesenchymal stromal cells mesothelioma malignant pleural mesothelioma (MPM) cell therapy Cytology
isfreeaccess_bool	true
container_title	Cells
authorswithroles_txt_mv	Valentina Coccè @@aut@@ Silvia La Monica @@aut@@ Mara Bonelli @@aut@@ Giulio Alessandri @@aut@@ Roberta Alfieri @@aut@@ Costanza Annamaria Lagrasta @@aut@@ Denise Madeddu @@aut@@ Caterina Frati @@aut@@ Lisa Flammini @@aut@@ Daniela Lisini @@aut@@ Angela Marcianti @@aut@@ Eugenio Parati @@aut@@ Francesca Paino @@aut@@ Aldo Giannì @@aut@@ Giampietro Farronato @@aut@@ Angela Falco @@aut@@ Lorenzo Spaggiari @@aut@@ Francesco Petrella @@aut@@ Augusto Pessina @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	718622081
id	DOAJ005921058
language_de	englisch
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callnumber-first	Q - Science
author	Valentina Coccè
spellingShingle	Valentina Coccè misc QH573-671 misc mesenchymal stromal cells misc mesothelioma misc malignant pleural mesothelioma (MPM) misc cell therapy misc Cytology Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells
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topic_title	QH573-671 Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells mesenchymal stromal cells mesothelioma malignant pleural mesothelioma (MPM) cell therapy
topic	misc QH573-671 misc mesenchymal stromal cells misc mesothelioma misc malignant pleural mesothelioma (MPM) misc cell therapy misc Cytology
topic_unstemmed	misc QH573-671 misc mesenchymal stromal cells misc mesothelioma misc malignant pleural mesothelioma (MPM) misc cell therapy misc Cytology
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title	Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells
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title_full	Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells
author_sort	Valentina Coccè
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author_browse	Valentina Coccè Silvia La Monica Mara Bonelli Giulio Alessandri Roberta Alfieri Costanza Annamaria Lagrasta Denise Madeddu Caterina Frati Lisa Flammini Daniela Lisini Angela Marcianti Eugenio Parati Francesca Paino Aldo Giannì Giampietro Farronato Angela Falco Lorenzo Spaggiari Francesco Petrella Augusto Pessina
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title_sort	inhibition of human malignant pleural mesothelioma growth by mesenchymal stromal cells
callnumber	QH573-671
title_auth	Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells
abstract	Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM. Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice. Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment. Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy.
abstractGer	Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM. Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice. Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment. Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy.
abstract_unstemmed	Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM. Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice. Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment. Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy.
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title_short	Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells
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author2	Silvia La Monica Mara Bonelli Giulio Alessandri Roberta Alfieri Costanza Annamaria Lagrasta Denise Madeddu Caterina Frati Lisa Flammini Daniela Lisini Angela Marcianti Eugenio Parati Francesca Paino Aldo Giannì Giampietro Farronato Angela Falco Lorenzo Spaggiari Francesco Petrella Augusto Pessina
author2Str	Silvia La Monica Mara Bonelli Giulio Alessandri Roberta Alfieri Costanza Annamaria Lagrasta Denise Madeddu Caterina Frati Lisa Flammini Daniela Lisini Angela Marcianti Eugenio Parati Francesca Paino Aldo Giannì Giampietro Farronato Angela Falco Lorenzo Spaggiari Francesco Petrella Augusto Pessina
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up_date	2024-07-03T17:54:34.349Z
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Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells

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