Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold

(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eig...
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Autor*in:	Jean-Claude Imber [verfasserIn] Andrea Roccuzzo [verfasserIn] Alexandra Stähli [verfasserIn] Nikola Saulacic [verfasserIn] James Deschner [verfasserIn] Anton Sculean [verfasserIn] Dieter Daniel Bosshardt [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	periodontal regeneration intrabony defect collagen scaffold volume-stable collagen matrix biomaterial histology

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 22(2021), 20, p 10915
Übergeordnetes Werk:	volume:22 ; year:2021 ; number:20, p 10915

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms222010915

Katalog-ID:	DOAJ00602145X

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allfields	10.3390/ijms222010915 doi (DE-627)DOAJ00602145X (DE-599)DOAJ0ae758420fd046cb8bfd13a2e9c82a49 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jean-Claude Imber verfasserin aut Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, <i<p</i< = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (<i<p</i< = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects. periodontal regeneration intrabony defect collagen scaffold volume-stable collagen matrix biomaterial histology Biology (General) Chemistry Andrea Roccuzzo verfasserin aut Alexandra Stähli verfasserin aut Nikola Saulacic verfasserin aut James Deschner verfasserin aut Anton Sculean verfasserin aut Dieter Daniel Bosshardt verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 20, p 10915 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:20, p 10915 https://doi.org/10.3390/ijms222010915 kostenfrei https://doaj.org/article/0ae758420fd046cb8bfd13a2e9c82a49 kostenfrei https://www.mdpi.com/1422-0067/22/20/10915 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 20, p 10915
spelling	10.3390/ijms222010915 doi (DE-627)DOAJ00602145X (DE-599)DOAJ0ae758420fd046cb8bfd13a2e9c82a49 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jean-Claude Imber verfasserin aut Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, <i<p</i< = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (<i<p</i< = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects. periodontal regeneration intrabony defect collagen scaffold volume-stable collagen matrix biomaterial histology Biology (General) Chemistry Andrea Roccuzzo verfasserin aut Alexandra Stähli verfasserin aut Nikola Saulacic verfasserin aut James Deschner verfasserin aut Anton Sculean verfasserin aut Dieter Daniel Bosshardt verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 20, p 10915 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:20, p 10915 https://doi.org/10.3390/ijms222010915 kostenfrei https://doaj.org/article/0ae758420fd046cb8bfd13a2e9c82a49 kostenfrei https://www.mdpi.com/1422-0067/22/20/10915 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 20, p 10915
allfields_unstemmed	10.3390/ijms222010915 doi (DE-627)DOAJ00602145X (DE-599)DOAJ0ae758420fd046cb8bfd13a2e9c82a49 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jean-Claude Imber verfasserin aut Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, <i<p</i< = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (<i<p</i< = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects. periodontal regeneration intrabony defect collagen scaffold volume-stable collagen matrix biomaterial histology Biology (General) Chemistry Andrea Roccuzzo verfasserin aut Alexandra Stähli verfasserin aut Nikola Saulacic verfasserin aut James Deschner verfasserin aut Anton Sculean verfasserin aut Dieter Daniel Bosshardt verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 20, p 10915 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:20, p 10915 https://doi.org/10.3390/ijms222010915 kostenfrei https://doaj.org/article/0ae758420fd046cb8bfd13a2e9c82a49 kostenfrei https://www.mdpi.com/1422-0067/22/20/10915 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 20, p 10915
allfieldsGer	10.3390/ijms222010915 doi (DE-627)DOAJ00602145X (DE-599)DOAJ0ae758420fd046cb8bfd13a2e9c82a49 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jean-Claude Imber verfasserin aut Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, <i<p</i< = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (<i<p</i< = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects. periodontal regeneration intrabony defect collagen scaffold volume-stable collagen matrix biomaterial histology Biology (General) Chemistry Andrea Roccuzzo verfasserin aut Alexandra Stähli verfasserin aut Nikola Saulacic verfasserin aut James Deschner verfasserin aut Anton Sculean verfasserin aut Dieter Daniel Bosshardt verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 20, p 10915 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:20, p 10915 https://doi.org/10.3390/ijms222010915 kostenfrei https://doaj.org/article/0ae758420fd046cb8bfd13a2e9c82a49 kostenfrei https://www.mdpi.com/1422-0067/22/20/10915 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 20, p 10915
allfieldsSound	10.3390/ijms222010915 doi (DE-627)DOAJ00602145X (DE-599)DOAJ0ae758420fd046cb8bfd13a2e9c82a49 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jean-Claude Imber verfasserin aut Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, <i<p</i< = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (<i<p</i< = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects. periodontal regeneration intrabony defect collagen scaffold volume-stable collagen matrix biomaterial histology Biology (General) Chemistry Andrea Roccuzzo verfasserin aut Alexandra Stähli verfasserin aut Nikola Saulacic verfasserin aut James Deschner verfasserin aut Anton Sculean verfasserin aut Dieter Daniel Bosshardt verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 20, p 10915 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:20, p 10915 https://doi.org/10.3390/ijms222010915 kostenfrei https://doaj.org/article/0ae758420fd046cb8bfd13a2e9c82a49 kostenfrei https://www.mdpi.com/1422-0067/22/20/10915 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 20, p 10915
language	English
source	In International Journal of Molecular Sciences 22(2021), 20, p 10915 volume:22 year:2021 number:20, p 10915
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callnumber-first	Q - Science
author	Jean-Claude Imber
spellingShingle	Jean-Claude Imber misc QH301-705.5 misc QD1-999 misc periodontal regeneration misc intrabony defect misc collagen scaffold misc volume-stable collagen matrix misc biomaterial misc histology misc Biology (General) misc Chemistry Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold
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topic_title	QH301-705.5 QD1-999 Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold periodontal regeneration intrabony defect collagen scaffold volume-stable collagen matrix biomaterial histology
topic	misc QH301-705.5 misc QD1-999 misc periodontal regeneration misc intrabony defect misc collagen scaffold misc volume-stable collagen matrix misc biomaterial misc histology misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc periodontal regeneration misc intrabony defect misc collagen scaffold misc volume-stable collagen matrix misc biomaterial misc histology misc Biology (General) misc Chemistry
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title_sort	immunohistochemical evaluation of periodontal regeneration using a porous collagen scaffold
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title_auth	Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold
abstract	(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, <i<p</i< = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (<i<p</i< = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects.
abstractGer	(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, <i<p</i< = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (<i<p</i< = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects.
abstract_unstemmed	(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, <i<p</i< = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (<i<p</i< = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects.
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title_short	Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold
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(2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, <i<p</i< = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (<i<p</i< = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. 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Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold

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