Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method

Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equip...
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Autor*in:	Xueyu Wang [verfasserIn] Xin Xu [verfasserIn] Wen Hu [verfasserIn] Kejing Zuo [verfasserIn] Zhili Li [verfasserIn] Yunchao Kan [verfasserIn] Lunguang Yao [verfasserIn] Jun Ji [verfasserIn] Yingzuo Bi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Reverse transcription polymerase spiral reaction (RT-PSR) Porcine epidemic diarrhea virus (PEDV) Sensitivity Specificity; detection

Übergeordnetes Werk:	In: BMC Veterinary Research - BMC, 2005, 15(2019), 1, Seite 7
Übergeordnetes Werk:	volume:15 ; year:2019 ; number:1 ; pages:7

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12917-019-1851-7

Katalog-ID:	DOAJ006206948

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520			\|a Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). For the assay, a specific RT-PSR primer pair was designed against a conserved region in PEDV ORF3. Results The RT-PSR was optimized, and PEDV could be detected after a 50 min incubation at 62 °C, in addition to the 15 min required for reverse transcription. No cross-reaction with other porcine infectious viruses was observed. This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. The positive rates for 65 clinical samples using the new RT-PSR assay and the conventional RT-PCR assay were 58.46% (38/65) and 53.84% (35/65), respectively. In the RT-PSR assay, the addition of a mixture of dyes allowed a positive reaction to be directly observed by the naked eye. Conclusions These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV.
650		4	\|a Reverse transcription polymerase spiral reaction (RT-PSR)
650		4	\|a Porcine epidemic diarrhea virus (PEDV)
650		4	\|a Sensitivity
650		4	\|a Specificity; detection
653		0	\|a Veterinary medicine
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allfields	10.1186/s12917-019-1851-7 doi (DE-627)DOAJ006206948 (DE-599)DOAJbe4b45ad4e75480fbe80b86b9b30d593 DE-627 ger DE-627 rakwb eng SF600-1100 Xueyu Wang verfasserin aut Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). For the assay, a specific RT-PSR primer pair was designed against a conserved region in PEDV ORF3. Results The RT-PSR was optimized, and PEDV could be detected after a 50 min incubation at 62 °C, in addition to the 15 min required for reverse transcription. No cross-reaction with other porcine infectious viruses was observed. This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. The positive rates for 65 clinical samples using the new RT-PSR assay and the conventional RT-PCR assay were 58.46% (38/65) and 53.84% (35/65), respectively. In the RT-PSR assay, the addition of a mixture of dyes allowed a positive reaction to be directly observed by the naked eye. Conclusions These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV. Reverse transcription polymerase spiral reaction (RT-PSR) Porcine epidemic diarrhea virus (PEDV) Sensitivity Specificity; detection Veterinary medicine Xin Xu verfasserin aut Wen Hu verfasserin aut Kejing Zuo verfasserin aut Zhili Li verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Jun Ji verfasserin aut Yingzuo Bi verfasserin aut In BMC Veterinary Research BMC, 2005 15(2019), 1, Seite 7 (DE-627)489256538 (DE-600)2191675-5 17466148 nnns volume:15 year:2019 number:1 pages:7 https://doi.org/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/article/be4b45ad4e75480fbe80b86b9b30d593 kostenfrei http://link.springer.com/article/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/toc/1746-6148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 7
spelling	10.1186/s12917-019-1851-7 doi (DE-627)DOAJ006206948 (DE-599)DOAJbe4b45ad4e75480fbe80b86b9b30d593 DE-627 ger DE-627 rakwb eng SF600-1100 Xueyu Wang verfasserin aut Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). For the assay, a specific RT-PSR primer pair was designed against a conserved region in PEDV ORF3. Results The RT-PSR was optimized, and PEDV could be detected after a 50 min incubation at 62 °C, in addition to the 15 min required for reverse transcription. No cross-reaction with other porcine infectious viruses was observed. This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. The positive rates for 65 clinical samples using the new RT-PSR assay and the conventional RT-PCR assay were 58.46% (38/65) and 53.84% (35/65), respectively. In the RT-PSR assay, the addition of a mixture of dyes allowed a positive reaction to be directly observed by the naked eye. Conclusions These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV. Reverse transcription polymerase spiral reaction (RT-PSR) Porcine epidemic diarrhea virus (PEDV) Sensitivity Specificity; detection Veterinary medicine Xin Xu verfasserin aut Wen Hu verfasserin aut Kejing Zuo verfasserin aut Zhili Li verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Jun Ji verfasserin aut Yingzuo Bi verfasserin aut In BMC Veterinary Research BMC, 2005 15(2019), 1, Seite 7 (DE-627)489256538 (DE-600)2191675-5 17466148 nnns volume:15 year:2019 number:1 pages:7 https://doi.org/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/article/be4b45ad4e75480fbe80b86b9b30d593 kostenfrei http://link.springer.com/article/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/toc/1746-6148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 7
allfields_unstemmed	10.1186/s12917-019-1851-7 doi (DE-627)DOAJ006206948 (DE-599)DOAJbe4b45ad4e75480fbe80b86b9b30d593 DE-627 ger DE-627 rakwb eng SF600-1100 Xueyu Wang verfasserin aut Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). For the assay, a specific RT-PSR primer pair was designed against a conserved region in PEDV ORF3. Results The RT-PSR was optimized, and PEDV could be detected after a 50 min incubation at 62 °C, in addition to the 15 min required for reverse transcription. No cross-reaction with other porcine infectious viruses was observed. This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. The positive rates for 65 clinical samples using the new RT-PSR assay and the conventional RT-PCR assay were 58.46% (38/65) and 53.84% (35/65), respectively. In the RT-PSR assay, the addition of a mixture of dyes allowed a positive reaction to be directly observed by the naked eye. Conclusions These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV. Reverse transcription polymerase spiral reaction (RT-PSR) Porcine epidemic diarrhea virus (PEDV) Sensitivity Specificity; detection Veterinary medicine Xin Xu verfasserin aut Wen Hu verfasserin aut Kejing Zuo verfasserin aut Zhili Li verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Jun Ji verfasserin aut Yingzuo Bi verfasserin aut In BMC Veterinary Research BMC, 2005 15(2019), 1, Seite 7 (DE-627)489256538 (DE-600)2191675-5 17466148 nnns volume:15 year:2019 number:1 pages:7 https://doi.org/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/article/be4b45ad4e75480fbe80b86b9b30d593 kostenfrei http://link.springer.com/article/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/toc/1746-6148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 7
allfieldsGer	10.1186/s12917-019-1851-7 doi (DE-627)DOAJ006206948 (DE-599)DOAJbe4b45ad4e75480fbe80b86b9b30d593 DE-627 ger DE-627 rakwb eng SF600-1100 Xueyu Wang verfasserin aut Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). For the assay, a specific RT-PSR primer pair was designed against a conserved region in PEDV ORF3. Results The RT-PSR was optimized, and PEDV could be detected after a 50 min incubation at 62 °C, in addition to the 15 min required for reverse transcription. No cross-reaction with other porcine infectious viruses was observed. This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. The positive rates for 65 clinical samples using the new RT-PSR assay and the conventional RT-PCR assay were 58.46% (38/65) and 53.84% (35/65), respectively. In the RT-PSR assay, the addition of a mixture of dyes allowed a positive reaction to be directly observed by the naked eye. Conclusions These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV. Reverse transcription polymerase spiral reaction (RT-PSR) Porcine epidemic diarrhea virus (PEDV) Sensitivity Specificity; detection Veterinary medicine Xin Xu verfasserin aut Wen Hu verfasserin aut Kejing Zuo verfasserin aut Zhili Li verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Jun Ji verfasserin aut Yingzuo Bi verfasserin aut In BMC Veterinary Research BMC, 2005 15(2019), 1, Seite 7 (DE-627)489256538 (DE-600)2191675-5 17466148 nnns volume:15 year:2019 number:1 pages:7 https://doi.org/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/article/be4b45ad4e75480fbe80b86b9b30d593 kostenfrei http://link.springer.com/article/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/toc/1746-6148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 7
allfieldsSound	10.1186/s12917-019-1851-7 doi (DE-627)DOAJ006206948 (DE-599)DOAJbe4b45ad4e75480fbe80b86b9b30d593 DE-627 ger DE-627 rakwb eng SF600-1100 Xueyu Wang verfasserin aut Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). For the assay, a specific RT-PSR primer pair was designed against a conserved region in PEDV ORF3. Results The RT-PSR was optimized, and PEDV could be detected after a 50 min incubation at 62 °C, in addition to the 15 min required for reverse transcription. No cross-reaction with other porcine infectious viruses was observed. This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. The positive rates for 65 clinical samples using the new RT-PSR assay and the conventional RT-PCR assay were 58.46% (38/65) and 53.84% (35/65), respectively. In the RT-PSR assay, the addition of a mixture of dyes allowed a positive reaction to be directly observed by the naked eye. Conclusions These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV. Reverse transcription polymerase spiral reaction (RT-PSR) Porcine epidemic diarrhea virus (PEDV) Sensitivity Specificity; detection Veterinary medicine Xin Xu verfasserin aut Wen Hu verfasserin aut Kejing Zuo verfasserin aut Zhili Li verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Jun Ji verfasserin aut Yingzuo Bi verfasserin aut In BMC Veterinary Research BMC, 2005 15(2019), 1, Seite 7 (DE-627)489256538 (DE-600)2191675-5 17466148 nnns volume:15 year:2019 number:1 pages:7 https://doi.org/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/article/be4b45ad4e75480fbe80b86b9b30d593 kostenfrei http://link.springer.com/article/10.1186/s12917-019-1851-7 kostenfrei https://doaj.org/toc/1746-6148 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2019 1 7
language	English
source	In BMC Veterinary Research 15(2019), 1, Seite 7 volume:15 year:2019 number:1 pages:7
sourceStr	In BMC Veterinary Research 15(2019), 1, Seite 7 volume:15 year:2019 number:1 pages:7
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Reverse transcription polymerase spiral reaction (RT-PSR) Porcine epidemic diarrhea virus (PEDV) Sensitivity Specificity; detection Veterinary medicine
isfreeaccess_bool	true
container_title	BMC Veterinary Research
authorswithroles_txt_mv	Xueyu Wang @@aut@@ Xin Xu @@aut@@ Wen Hu @@aut@@ Kejing Zuo @@aut@@ Zhili Li @@aut@@ Yunchao Kan @@aut@@ Lunguang Yao @@aut@@ Jun Ji @@aut@@ Yingzuo Bi @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	489256538
id	DOAJ006206948
language_de	englisch
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callnumber-first	S - Agriculture
author	Xueyu Wang
spellingShingle	Xueyu Wang misc SF600-1100 misc Reverse transcription polymerase spiral reaction (RT-PSR) misc Porcine epidemic diarrhea virus (PEDV) misc Sensitivity misc Specificity; detection misc Veterinary medicine Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method
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topic_title	SF600-1100 Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method Reverse transcription polymerase spiral reaction (RT-PSR) Porcine epidemic diarrhea virus (PEDV) Sensitivity Specificity; detection
topic	misc SF600-1100 misc Reverse transcription polymerase spiral reaction (RT-PSR) misc Porcine epidemic diarrhea virus (PEDV) misc Sensitivity misc Specificity; detection misc Veterinary medicine
topic_unstemmed	misc SF600-1100 misc Reverse transcription polymerase spiral reaction (RT-PSR) misc Porcine epidemic diarrhea virus (PEDV) misc Sensitivity misc Specificity; detection misc Veterinary medicine
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title	Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method
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title_full	Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method
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author_browse	Xueyu Wang Xin Xu Wen Hu Kejing Zuo Zhili Li Yunchao Kan Lunguang Yao Jun Ji Yingzuo Bi
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title_sort	visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method
callnumber	SF600-1100
title_auth	Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method
abstract	Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). For the assay, a specific RT-PSR primer pair was designed against a conserved region in PEDV ORF3. Results The RT-PSR was optimized, and PEDV could be detected after a 50 min incubation at 62 °C, in addition to the 15 min required for reverse transcription. No cross-reaction with other porcine infectious viruses was observed. This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. The positive rates for 65 clinical samples using the new RT-PSR assay and the conventional RT-PCR assay were 58.46% (38/65) and 53.84% (35/65), respectively. In the RT-PSR assay, the addition of a mixture of dyes allowed a positive reaction to be directly observed by the naked eye. Conclusions These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV.
abstractGer	Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). For the assay, a specific RT-PSR primer pair was designed against a conserved region in PEDV ORF3. Results The RT-PSR was optimized, and PEDV could be detected after a 50 min incubation at 62 °C, in addition to the 15 min required for reverse transcription. No cross-reaction with other porcine infectious viruses was observed. This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. The positive rates for 65 clinical samples using the new RT-PSR assay and the conventional RT-PCR assay were 58.46% (38/65) and 53.84% (35/65), respectively. In the RT-PSR assay, the addition of a mixture of dyes allowed a positive reaction to be directly observed by the naked eye. Conclusions These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV.
abstract_unstemmed	Abstract Background Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). For the assay, a specific RT-PSR primer pair was designed against a conserved region in PEDV ORF3. Results The RT-PSR was optimized, and PEDV could be detected after a 50 min incubation at 62 °C, in addition to the 15 min required for reverse transcription. No cross-reaction with other porcine infectious viruses was observed. This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. The positive rates for 65 clinical samples using the new RT-PSR assay and the conventional RT-PCR assay were 58.46% (38/65) and 53.84% (35/65), respectively. In the RT-PSR assay, the addition of a mixture of dyes allowed a positive reaction to be directly observed by the naked eye. Conclusions These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV.
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title_short	Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method
url	https://doi.org/10.1186/s12917-019-1851-7 https://doaj.org/article/be4b45ad4e75480fbe80b86b9b30d593 http://link.springer.com/article/10.1186/s12917-019-1851-7 https://doaj.org/toc/1746-6148
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Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method

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