Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome

The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study include...
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Autor*in:	Antonio Cano-Ortiz [verfasserIn] Aurora Laborda-Illanes [verfasserIn] Isaac Plaza-Andrades [verfasserIn] Alberto Membrillo del Pozo [verfasserIn] Alberto Villarrubia Cuadrado [verfasserIn] Marina Rodríguez Calvo de Mora [verfasserIn] Isabel Leiva-Gea [verfasserIn] Lidia Sanchez-Alcoholado [verfasserIn] María Isabel Queipo-Ortuño [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	primary Sjögren’s syndrome gut microbiota inflammation intestinal permeability FOXP3 expression

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 21(2020), 22, p 8733
Übergeordnetes Werk:	volume:21 ; year:2020 ; number:22, p 8733

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms21228733

Katalog-ID:	DOAJ006480276

Internformat


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author_variant	a c o aco a l i ali i p a ipa a m d p amdp a v c avc m r c d m mrcdm i l g ilg l s a lsa m i q o miqo
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allfields	10.3390/ijms21228733 doi (DE-627)DOAJ006480276 (DE-599)DOAJ382c5bc2b0b943629a1734b202272cf8 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Antonio Cano-Ortiz verfasserin aut Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation. primary Sjögren’s syndrome gut microbiota inflammation intestinal permeability FOXP3 expression Biology (General) Chemistry Aurora Laborda-Illanes verfasserin aut Isaac Plaza-Andrades verfasserin aut Alberto Membrillo del Pozo verfasserin aut Alberto Villarrubia Cuadrado verfasserin aut Marina Rodríguez Calvo de Mora verfasserin aut Isabel Leiva-Gea verfasserin aut Lidia Sanchez-Alcoholado verfasserin aut María Isabel Queipo-Ortuño verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8733 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8733 https://doi.org/10.3390/ijms21228733 kostenfrei https://doaj.org/article/382c5bc2b0b943629a1734b202272cf8 kostenfrei https://www.mdpi.com/1422-0067/21/22/8733 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8733
spelling	10.3390/ijms21228733 doi (DE-627)DOAJ006480276 (DE-599)DOAJ382c5bc2b0b943629a1734b202272cf8 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Antonio Cano-Ortiz verfasserin aut Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation. primary Sjögren’s syndrome gut microbiota inflammation intestinal permeability FOXP3 expression Biology (General) Chemistry Aurora Laborda-Illanes verfasserin aut Isaac Plaza-Andrades verfasserin aut Alberto Membrillo del Pozo verfasserin aut Alberto Villarrubia Cuadrado verfasserin aut Marina Rodríguez Calvo de Mora verfasserin aut Isabel Leiva-Gea verfasserin aut Lidia Sanchez-Alcoholado verfasserin aut María Isabel Queipo-Ortuño verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8733 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8733 https://doi.org/10.3390/ijms21228733 kostenfrei https://doaj.org/article/382c5bc2b0b943629a1734b202272cf8 kostenfrei https://www.mdpi.com/1422-0067/21/22/8733 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8733
allfields_unstemmed	10.3390/ijms21228733 doi (DE-627)DOAJ006480276 (DE-599)DOAJ382c5bc2b0b943629a1734b202272cf8 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Antonio Cano-Ortiz verfasserin aut Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation. primary Sjögren’s syndrome gut microbiota inflammation intestinal permeability FOXP3 expression Biology (General) Chemistry Aurora Laborda-Illanes verfasserin aut Isaac Plaza-Andrades verfasserin aut Alberto Membrillo del Pozo verfasserin aut Alberto Villarrubia Cuadrado verfasserin aut Marina Rodríguez Calvo de Mora verfasserin aut Isabel Leiva-Gea verfasserin aut Lidia Sanchez-Alcoholado verfasserin aut María Isabel Queipo-Ortuño verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8733 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8733 https://doi.org/10.3390/ijms21228733 kostenfrei https://doaj.org/article/382c5bc2b0b943629a1734b202272cf8 kostenfrei https://www.mdpi.com/1422-0067/21/22/8733 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8733
allfieldsGer	10.3390/ijms21228733 doi (DE-627)DOAJ006480276 (DE-599)DOAJ382c5bc2b0b943629a1734b202272cf8 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Antonio Cano-Ortiz verfasserin aut Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation. primary Sjögren’s syndrome gut microbiota inflammation intestinal permeability FOXP3 expression Biology (General) Chemistry Aurora Laborda-Illanes verfasserin aut Isaac Plaza-Andrades verfasserin aut Alberto Membrillo del Pozo verfasserin aut Alberto Villarrubia Cuadrado verfasserin aut Marina Rodríguez Calvo de Mora verfasserin aut Isabel Leiva-Gea verfasserin aut Lidia Sanchez-Alcoholado verfasserin aut María Isabel Queipo-Ortuño verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8733 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8733 https://doi.org/10.3390/ijms21228733 kostenfrei https://doaj.org/article/382c5bc2b0b943629a1734b202272cf8 kostenfrei https://www.mdpi.com/1422-0067/21/22/8733 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8733
allfieldsSound	10.3390/ijms21228733 doi (DE-627)DOAJ006480276 (DE-599)DOAJ382c5bc2b0b943629a1734b202272cf8 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Antonio Cano-Ortiz verfasserin aut Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation. primary Sjögren’s syndrome gut microbiota inflammation intestinal permeability FOXP3 expression Biology (General) Chemistry Aurora Laborda-Illanes verfasserin aut Isaac Plaza-Andrades verfasserin aut Alberto Membrillo del Pozo verfasserin aut Alberto Villarrubia Cuadrado verfasserin aut Marina Rodríguez Calvo de Mora verfasserin aut Isabel Leiva-Gea verfasserin aut Lidia Sanchez-Alcoholado verfasserin aut María Isabel Queipo-Ortuño verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8733 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8733 https://doi.org/10.3390/ijms21228733 kostenfrei https://doaj.org/article/382c5bc2b0b943629a1734b202272cf8 kostenfrei https://www.mdpi.com/1422-0067/21/22/8733 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8733
language	English
source	In International Journal of Molecular Sciences 21(2020), 22, p 8733 volume:21 year:2020 number:22, p 8733
sourceStr	In International Journal of Molecular Sciences 21(2020), 22, p 8733 volume:21 year:2020 number:22, p 8733
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	primary Sjögren’s syndrome gut microbiota inflammation intestinal permeability FOXP3 expression Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Antonio Cano-Ortiz @@aut@@ Aurora Laborda-Illanes @@aut@@ Isaac Plaza-Andrades @@aut@@ Alberto Membrillo del Pozo @@aut@@ Alberto Villarrubia Cuadrado @@aut@@ Marina Rodríguez Calvo de Mora @@aut@@ Isabel Leiva-Gea @@aut@@ Lidia Sanchez-Alcoholado @@aut@@ María Isabel Queipo-Ortuño @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	316340715
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language_de	englisch
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callnumber-first	Q - Science
author	Antonio Cano-Ortiz
spellingShingle	Antonio Cano-Ortiz misc QH301-705.5 misc QD1-999 misc primary Sjögren’s syndrome misc gut microbiota misc inflammation misc intestinal permeability misc FOXP3 expression misc Biology (General) misc Chemistry Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome
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topic_title	QH301-705.5 QD1-999 Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome primary Sjögren’s syndrome gut microbiota inflammation intestinal permeability FOXP3 expression
topic	misc QH301-705.5 misc QD1-999 misc primary Sjögren’s syndrome misc gut microbiota misc inflammation misc intestinal permeability misc FOXP3 expression misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc primary Sjögren’s syndrome misc gut microbiota misc inflammation misc intestinal permeability misc FOXP3 expression misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc primary Sjögren’s syndrome misc gut microbiota misc inflammation misc intestinal permeability misc FOXP3 expression misc Biology (General) misc Chemistry
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title	Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome
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title_full	Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome
author_sort	Antonio Cano-Ortiz
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author_browse	Antonio Cano-Ortiz Aurora Laborda-Illanes Isaac Plaza-Andrades Alberto Membrillo del Pozo Alberto Villarrubia Cuadrado Marina Rodríguez Calvo de Mora Isabel Leiva-Gea Lidia Sanchez-Alcoholado María Isabel Queipo-Ortuño
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title_sort	connection between the gut microbiome, systemic inflammation, gut permeability and foxp3 expression in patients with primary sjögren’s syndrome
callnumber	QH301-705.5
title_auth	Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome
abstract	The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation.
abstractGer	The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation.
abstract_unstemmed	The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation.
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title_short	Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome
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