Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe
We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunoh...
Ausführliche Beschreibung
Autor*in: |
Aran Shintani [verfasserIn] Masashi Higuchi [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2021 |
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Schlagwörter: |
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Übergeordnetes Werk: |
In: Stem Cell Research - Elsevier, 2015, 52(2021), Seite 102223- |
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Übergeordnetes Werk: |
volume:52 ; year:2021 ; pages:102223- |
Links: |
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DOI / URN: |
10.1016/j.scr.2021.102223 |
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Katalog-ID: |
DOAJ006549942 |
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520 | |a We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunohistochemical analysis revealed that PRRX1/SOX2-positive cells were located mainly in the marginal cell layer (MCL)-niche of the adult mouse pituitary gland, but were rarely present in the parenchymal-niche. Two-dimensional cultivation of primary cells from the anterior lobe (AL), including the MCL-niche, achieved efficient proliferation of PRRX1/SOX2-positive cells with high expression levels of pituitary stem/progenitor cell and niche markers. In contrast, primary cells from the AL, excluding the MCL-niche, showed limited growth. When isolated PRRX1/SOX2-positive cells and clusters of the parenchymal-niche were compared immunocytochemically, aquaporin 5, a marker of the MCL-niche, was detected only in isolated cells. Three-dimensional cultivation of isolated PRRX1/SOX2-positive cells promoted the formation of cystic-like spheroids and differentiation into endocrine cells. Thus, PRRX1-positive adult pituitary stem/progenitor cells were successfully isolated from the MCL-niche. The present study provides a powerful tool to analyze the cell supply system in the pituitary gland. | ||
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10.1016/j.scr.2021.102223 doi (DE-627)DOAJ006549942 (DE-599)DOAJ76db43cdbe8849a680b432c2b5d63025 DE-627 ger DE-627 rakwb eng QH301-705.5 Aran Shintani verfasserin aut Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunohistochemical analysis revealed that PRRX1/SOX2-positive cells were located mainly in the marginal cell layer (MCL)-niche of the adult mouse pituitary gland, but were rarely present in the parenchymal-niche. Two-dimensional cultivation of primary cells from the anterior lobe (AL), including the MCL-niche, achieved efficient proliferation of PRRX1/SOX2-positive cells with high expression levels of pituitary stem/progenitor cell and niche markers. In contrast, primary cells from the AL, excluding the MCL-niche, showed limited growth. When isolated PRRX1/SOX2-positive cells and clusters of the parenchymal-niche were compared immunocytochemically, aquaporin 5, a marker of the MCL-niche, was detected only in isolated cells. Three-dimensional cultivation of isolated PRRX1/SOX2-positive cells promoted the formation of cystic-like spheroids and differentiation into endocrine cells. Thus, PRRX1-positive adult pituitary stem/progenitor cells were successfully isolated from the MCL-niche. The present study provides a powerful tool to analyze the cell supply system in the pituitary gland. Mouse Pituitary gland Stem/progenitor cell PRRX1 Marginal cell layer Biology (General) Masashi Higuchi verfasserin aut In Stem Cell Research Elsevier, 2015 52(2021), Seite 102223- (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:52 year:2021 pages:102223- https://doi.org/10.1016/j.scr.2021.102223 kostenfrei https://doaj.org/article/76db43cdbe8849a680b432c2b5d63025 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506121000696 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 52 2021 102223- |
spelling |
10.1016/j.scr.2021.102223 doi (DE-627)DOAJ006549942 (DE-599)DOAJ76db43cdbe8849a680b432c2b5d63025 DE-627 ger DE-627 rakwb eng QH301-705.5 Aran Shintani verfasserin aut Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunohistochemical analysis revealed that PRRX1/SOX2-positive cells were located mainly in the marginal cell layer (MCL)-niche of the adult mouse pituitary gland, but were rarely present in the parenchymal-niche. Two-dimensional cultivation of primary cells from the anterior lobe (AL), including the MCL-niche, achieved efficient proliferation of PRRX1/SOX2-positive cells with high expression levels of pituitary stem/progenitor cell and niche markers. In contrast, primary cells from the AL, excluding the MCL-niche, showed limited growth. When isolated PRRX1/SOX2-positive cells and clusters of the parenchymal-niche were compared immunocytochemically, aquaporin 5, a marker of the MCL-niche, was detected only in isolated cells. Three-dimensional cultivation of isolated PRRX1/SOX2-positive cells promoted the formation of cystic-like spheroids and differentiation into endocrine cells. Thus, PRRX1-positive adult pituitary stem/progenitor cells were successfully isolated from the MCL-niche. The present study provides a powerful tool to analyze the cell supply system in the pituitary gland. Mouse Pituitary gland Stem/progenitor cell PRRX1 Marginal cell layer Biology (General) Masashi Higuchi verfasserin aut In Stem Cell Research Elsevier, 2015 52(2021), Seite 102223- (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:52 year:2021 pages:102223- https://doi.org/10.1016/j.scr.2021.102223 kostenfrei https://doaj.org/article/76db43cdbe8849a680b432c2b5d63025 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506121000696 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 52 2021 102223- |
allfields_unstemmed |
10.1016/j.scr.2021.102223 doi (DE-627)DOAJ006549942 (DE-599)DOAJ76db43cdbe8849a680b432c2b5d63025 DE-627 ger DE-627 rakwb eng QH301-705.5 Aran Shintani verfasserin aut Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunohistochemical analysis revealed that PRRX1/SOX2-positive cells were located mainly in the marginal cell layer (MCL)-niche of the adult mouse pituitary gland, but were rarely present in the parenchymal-niche. Two-dimensional cultivation of primary cells from the anterior lobe (AL), including the MCL-niche, achieved efficient proliferation of PRRX1/SOX2-positive cells with high expression levels of pituitary stem/progenitor cell and niche markers. In contrast, primary cells from the AL, excluding the MCL-niche, showed limited growth. When isolated PRRX1/SOX2-positive cells and clusters of the parenchymal-niche were compared immunocytochemically, aquaporin 5, a marker of the MCL-niche, was detected only in isolated cells. Three-dimensional cultivation of isolated PRRX1/SOX2-positive cells promoted the formation of cystic-like spheroids and differentiation into endocrine cells. Thus, PRRX1-positive adult pituitary stem/progenitor cells were successfully isolated from the MCL-niche. The present study provides a powerful tool to analyze the cell supply system in the pituitary gland. Mouse Pituitary gland Stem/progenitor cell PRRX1 Marginal cell layer Biology (General) Masashi Higuchi verfasserin aut In Stem Cell Research Elsevier, 2015 52(2021), Seite 102223- (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:52 year:2021 pages:102223- https://doi.org/10.1016/j.scr.2021.102223 kostenfrei https://doaj.org/article/76db43cdbe8849a680b432c2b5d63025 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506121000696 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 52 2021 102223- |
allfieldsGer |
10.1016/j.scr.2021.102223 doi (DE-627)DOAJ006549942 (DE-599)DOAJ76db43cdbe8849a680b432c2b5d63025 DE-627 ger DE-627 rakwb eng QH301-705.5 Aran Shintani verfasserin aut Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunohistochemical analysis revealed that PRRX1/SOX2-positive cells were located mainly in the marginal cell layer (MCL)-niche of the adult mouse pituitary gland, but were rarely present in the parenchymal-niche. Two-dimensional cultivation of primary cells from the anterior lobe (AL), including the MCL-niche, achieved efficient proliferation of PRRX1/SOX2-positive cells with high expression levels of pituitary stem/progenitor cell and niche markers. In contrast, primary cells from the AL, excluding the MCL-niche, showed limited growth. When isolated PRRX1/SOX2-positive cells and clusters of the parenchymal-niche were compared immunocytochemically, aquaporin 5, a marker of the MCL-niche, was detected only in isolated cells. Three-dimensional cultivation of isolated PRRX1/SOX2-positive cells promoted the formation of cystic-like spheroids and differentiation into endocrine cells. Thus, PRRX1-positive adult pituitary stem/progenitor cells were successfully isolated from the MCL-niche. The present study provides a powerful tool to analyze the cell supply system in the pituitary gland. Mouse Pituitary gland Stem/progenitor cell PRRX1 Marginal cell layer Biology (General) Masashi Higuchi verfasserin aut In Stem Cell Research Elsevier, 2015 52(2021), Seite 102223- (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:52 year:2021 pages:102223- https://doi.org/10.1016/j.scr.2021.102223 kostenfrei https://doaj.org/article/76db43cdbe8849a680b432c2b5d63025 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506121000696 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 52 2021 102223- |
allfieldsSound |
10.1016/j.scr.2021.102223 doi (DE-627)DOAJ006549942 (DE-599)DOAJ76db43cdbe8849a680b432c2b5d63025 DE-627 ger DE-627 rakwb eng QH301-705.5 Aran Shintani verfasserin aut Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunohistochemical analysis revealed that PRRX1/SOX2-positive cells were located mainly in the marginal cell layer (MCL)-niche of the adult mouse pituitary gland, but were rarely present in the parenchymal-niche. Two-dimensional cultivation of primary cells from the anterior lobe (AL), including the MCL-niche, achieved efficient proliferation of PRRX1/SOX2-positive cells with high expression levels of pituitary stem/progenitor cell and niche markers. In contrast, primary cells from the AL, excluding the MCL-niche, showed limited growth. When isolated PRRX1/SOX2-positive cells and clusters of the parenchymal-niche were compared immunocytochemically, aquaporin 5, a marker of the MCL-niche, was detected only in isolated cells. Three-dimensional cultivation of isolated PRRX1/SOX2-positive cells promoted the formation of cystic-like spheroids and differentiation into endocrine cells. Thus, PRRX1-positive adult pituitary stem/progenitor cells were successfully isolated from the MCL-niche. The present study provides a powerful tool to analyze the cell supply system in the pituitary gland. Mouse Pituitary gland Stem/progenitor cell PRRX1 Marginal cell layer Biology (General) Masashi Higuchi verfasserin aut In Stem Cell Research Elsevier, 2015 52(2021), Seite 102223- (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:52 year:2021 pages:102223- https://doi.org/10.1016/j.scr.2021.102223 kostenfrei https://doaj.org/article/76db43cdbe8849a680b432c2b5d63025 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506121000696 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 52 2021 102223- |
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Aran Shintani |
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Aran Shintani misc QH301-705.5 misc Mouse misc Pituitary gland misc Stem/progenitor cell misc PRRX1 misc Marginal cell layer misc Biology (General) Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe |
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QH301-705.5 Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe Mouse Pituitary gland Stem/progenitor cell PRRX1 Marginal cell layer |
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misc QH301-705.5 misc Mouse misc Pituitary gland misc Stem/progenitor cell misc PRRX1 misc Marginal cell layer misc Biology (General) |
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Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe |
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Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe |
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Aran Shintani |
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Stem Cell Research |
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isolation of prrx1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe |
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Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe |
abstract |
We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunohistochemical analysis revealed that PRRX1/SOX2-positive cells were located mainly in the marginal cell layer (MCL)-niche of the adult mouse pituitary gland, but were rarely present in the parenchymal-niche. Two-dimensional cultivation of primary cells from the anterior lobe (AL), including the MCL-niche, achieved efficient proliferation of PRRX1/SOX2-positive cells with high expression levels of pituitary stem/progenitor cell and niche markers. In contrast, primary cells from the AL, excluding the MCL-niche, showed limited growth. When isolated PRRX1/SOX2-positive cells and clusters of the parenchymal-niche were compared immunocytochemically, aquaporin 5, a marker of the MCL-niche, was detected only in isolated cells. Three-dimensional cultivation of isolated PRRX1/SOX2-positive cells promoted the formation of cystic-like spheroids and differentiation into endocrine cells. Thus, PRRX1-positive adult pituitary stem/progenitor cells were successfully isolated from the MCL-niche. The present study provides a powerful tool to analyze the cell supply system in the pituitary gland. |
abstractGer |
We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunohistochemical analysis revealed that PRRX1/SOX2-positive cells were located mainly in the marginal cell layer (MCL)-niche of the adult mouse pituitary gland, but were rarely present in the parenchymal-niche. Two-dimensional cultivation of primary cells from the anterior lobe (AL), including the MCL-niche, achieved efficient proliferation of PRRX1/SOX2-positive cells with high expression levels of pituitary stem/progenitor cell and niche markers. In contrast, primary cells from the AL, excluding the MCL-niche, showed limited growth. When isolated PRRX1/SOX2-positive cells and clusters of the parenchymal-niche were compared immunocytochemically, aquaporin 5, a marker of the MCL-niche, was detected only in isolated cells. Three-dimensional cultivation of isolated PRRX1/SOX2-positive cells promoted the formation of cystic-like spheroids and differentiation into endocrine cells. Thus, PRRX1-positive adult pituitary stem/progenitor cells were successfully isolated from the MCL-niche. The present study provides a powerful tool to analyze the cell supply system in the pituitary gland. |
abstract_unstemmed |
We recently suggested that paired-related homeobox 1 (PRRX1) and sex-determining region Y-box 2 (SOX2) double-positive (PRRX1/SOX2-positive) cells are pituitary stem/progenitor cells. The present study aimed to isolate PRRX1-positive cells located in the pituitary stem/progenitor cell niche. Immunohistochemical analysis revealed that PRRX1/SOX2-positive cells were located mainly in the marginal cell layer (MCL)-niche of the adult mouse pituitary gland, but were rarely present in the parenchymal-niche. Two-dimensional cultivation of primary cells from the anterior lobe (AL), including the MCL-niche, achieved efficient proliferation of PRRX1/SOX2-positive cells with high expression levels of pituitary stem/progenitor cell and niche markers. In contrast, primary cells from the AL, excluding the MCL-niche, showed limited growth. When isolated PRRX1/SOX2-positive cells and clusters of the parenchymal-niche were compared immunocytochemically, aquaporin 5, a marker of the MCL-niche, was detected only in isolated cells. Three-dimensional cultivation of isolated PRRX1/SOX2-positive cells promoted the formation of cystic-like spheroids and differentiation into endocrine cells. Thus, PRRX1-positive adult pituitary stem/progenitor cells were successfully isolated from the MCL-niche. The present study provides a powerful tool to analyze the cell supply system in the pituitary gland. |
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title_short |
Isolation of PRRX1-positive adult pituitary stem/progenitor cells from the marginal cell layer of the mouse anterior lobe |
url |
https://doi.org/10.1016/j.scr.2021.102223 https://doaj.org/article/76db43cdbe8849a680b432c2b5d63025 http://www.sciencedirect.com/science/article/pii/S1873506121000696 https://doaj.org/toc/1873-5061 |
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score |
7.400728 |