In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects

Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential biological processes. However, many common methods...
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Autor*in:	Sihuai Sun [verfasserIn] Xiaobing Yang [verfasserIn] Yao Wang [verfasserIn] Xihui Shen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	protein–protein interactions bioluminescence fluorescence Bioluminescence Resonance Energy Transfer Förster Resonance Energy Transfer NanoLuc bacterial luciferase protein dynamics

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 17(2016), 10, p 1704
Übergeordnetes Werk:	volume:17 ; year:2016 ; number:10, p 1704

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms17101704

Katalog-ID:	DOAJ006774369

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520			\|a Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential biological processes. However, many common methods for PPI investigations are slightly unreliable and suffer from various limitations, especially in the studies of dynamic PPIs. To solve this problem, a method called Bioluminescence Resonance Energy Transfer (BRET) was developed about seventeen years ago. Since then, BRET has evolved into a whole class of methods that can be used to survey virtually any kinds of PPIs. Compared to many traditional methods, BRET is highly sensitive, reliable, easy to perform, and relatively inexpensive. However, most importantly, it can be done in vivo and allows the real-time monitoring of dynamic PPIs with the easily detectable light signal, which is extremely valuable for the PPI functional research. This review will take a comprehensive look at this powerful technique, including its principles, comparisons with other methods, experimental approaches, classifications, applications, early developments, recent progress, and prospects.
650		4	\|a protein–protein interactions
650		4	\|a bioluminescence
650		4	\|a fluorescence
650		4	\|a Bioluminescence Resonance Energy Transfer
650		4	\|a Förster Resonance Energy Transfer
650		4	\|a NanoLuc
650		4	\|a bacterial luciferase
650		4	\|a protein dynamics
653		0	\|a Biology (General)
653		0	\|a Chemistry
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spelling	10.3390/ijms17101704 doi (DE-627)DOAJ006774369 (DE-599)DOAJ82cd917e24ab498b901c940523113442 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Sihuai Sun verfasserin aut In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential biological processes. However, many common methods for PPI investigations are slightly unreliable and suffer from various limitations, especially in the studies of dynamic PPIs. To solve this problem, a method called Bioluminescence Resonance Energy Transfer (BRET) was developed about seventeen years ago. Since then, BRET has evolved into a whole class of methods that can be used to survey virtually any kinds of PPIs. Compared to many traditional methods, BRET is highly sensitive, reliable, easy to perform, and relatively inexpensive. However, most importantly, it can be done in vivo and allows the real-time monitoring of dynamic PPIs with the easily detectable light signal, which is extremely valuable for the PPI functional research. This review will take a comprehensive look at this powerful technique, including its principles, comparisons with other methods, experimental approaches, classifications, applications, early developments, recent progress, and prospects. protein–protein interactions bioluminescence fluorescence Bioluminescence Resonance Energy Transfer Förster Resonance Energy Transfer NanoLuc bacterial luciferase protein dynamics Biology (General) Chemistry Xiaobing Yang verfasserin aut Yao Wang verfasserin aut Xihui Shen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 17(2016), 10, p 1704 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:17 year:2016 number:10, p 1704 https://doi.org/10.3390/ijms17101704 kostenfrei https://doaj.org/article/82cd917e24ab498b901c940523113442 kostenfrei http://www.mdpi.com/1422-0067/17/10/1704 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2016 10, p 1704
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allfieldsSound	10.3390/ijms17101704 doi (DE-627)DOAJ006774369 (DE-599)DOAJ82cd917e24ab498b901c940523113442 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Sihuai Sun verfasserin aut In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential biological processes. However, many common methods for PPI investigations are slightly unreliable and suffer from various limitations, especially in the studies of dynamic PPIs. To solve this problem, a method called Bioluminescence Resonance Energy Transfer (BRET) was developed about seventeen years ago. Since then, BRET has evolved into a whole class of methods that can be used to survey virtually any kinds of PPIs. Compared to many traditional methods, BRET is highly sensitive, reliable, easy to perform, and relatively inexpensive. However, most importantly, it can be done in vivo and allows the real-time monitoring of dynamic PPIs with the easily detectable light signal, which is extremely valuable for the PPI functional research. This review will take a comprehensive look at this powerful technique, including its principles, comparisons with other methods, experimental approaches, classifications, applications, early developments, recent progress, and prospects. protein–protein interactions bioluminescence fluorescence Bioluminescence Resonance Energy Transfer Förster Resonance Energy Transfer NanoLuc bacterial luciferase protein dynamics Biology (General) Chemistry Xiaobing Yang verfasserin aut Yao Wang verfasserin aut Xihui Shen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 17(2016), 10, p 1704 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:17 year:2016 number:10, p 1704 https://doi.org/10.3390/ijms17101704 kostenfrei https://doaj.org/article/82cd917e24ab498b901c940523113442 kostenfrei http://www.mdpi.com/1422-0067/17/10/1704 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2016 10, p 1704
language	English
source	In International Journal of Molecular Sciences 17(2016), 10, p 1704 volume:17 year:2016 number:10, p 1704
sourceStr	In International Journal of Molecular Sciences 17(2016), 10, p 1704 volume:17 year:2016 number:10, p 1704
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topic_facet	protein–protein interactions bioluminescence fluorescence Bioluminescence Resonance Energy Transfer Förster Resonance Energy Transfer NanoLuc bacterial luciferase protein dynamics Biology (General) Chemistry
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container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Sihuai Sun @@aut@@ Xiaobing Yang @@aut@@ Yao Wang @@aut@@ Xihui Shen @@aut@@
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callnumber-first	Q - Science
author	Sihuai Sun
spellingShingle	Sihuai Sun misc QH301-705.5 misc QD1-999 misc protein–protein interactions misc bioluminescence misc fluorescence misc Bioluminescence Resonance Energy Transfer misc Förster Resonance Energy Transfer misc NanoLuc misc bacterial luciferase misc protein dynamics misc Biology (General) misc Chemistry In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects
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topic_title	QH301-705.5 QD1-999 In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects protein–protein interactions bioluminescence fluorescence Bioluminescence Resonance Energy Transfer Förster Resonance Energy Transfer NanoLuc bacterial luciferase protein dynamics
topic	misc QH301-705.5 misc QD1-999 misc protein–protein interactions misc bioluminescence misc fluorescence misc Bioluminescence Resonance Energy Transfer misc Förster Resonance Energy Transfer misc NanoLuc misc bacterial luciferase misc protein dynamics misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc protein–protein interactions misc bioluminescence misc fluorescence misc Bioluminescence Resonance Energy Transfer misc Förster Resonance Energy Transfer misc NanoLuc misc bacterial luciferase misc protein dynamics misc Biology (General) misc Chemistry
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title	In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects
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title_sort	in vivo analysis of protein–protein interactions with bioluminescence resonance energy transfer (bret): progress and prospects
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title_auth	In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects
abstract	Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential biological processes. However, many common methods for PPI investigations are slightly unreliable and suffer from various limitations, especially in the studies of dynamic PPIs. To solve this problem, a method called Bioluminescence Resonance Energy Transfer (BRET) was developed about seventeen years ago. Since then, BRET has evolved into a whole class of methods that can be used to survey virtually any kinds of PPIs. Compared to many traditional methods, BRET is highly sensitive, reliable, easy to perform, and relatively inexpensive. However, most importantly, it can be done in vivo and allows the real-time monitoring of dynamic PPIs with the easily detectable light signal, which is extremely valuable for the PPI functional research. This review will take a comprehensive look at this powerful technique, including its principles, comparisons with other methods, experimental approaches, classifications, applications, early developments, recent progress, and prospects.
abstractGer	Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential biological processes. However, many common methods for PPI investigations are slightly unreliable and suffer from various limitations, especially in the studies of dynamic PPIs. To solve this problem, a method called Bioluminescence Resonance Energy Transfer (BRET) was developed about seventeen years ago. Since then, BRET has evolved into a whole class of methods that can be used to survey virtually any kinds of PPIs. Compared to many traditional methods, BRET is highly sensitive, reliable, easy to perform, and relatively inexpensive. However, most importantly, it can be done in vivo and allows the real-time monitoring of dynamic PPIs with the easily detectable light signal, which is extremely valuable for the PPI functional research. This review will take a comprehensive look at this powerful technique, including its principles, comparisons with other methods, experimental approaches, classifications, applications, early developments, recent progress, and prospects.
abstract_unstemmed	Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential biological processes. However, many common methods for PPI investigations are slightly unreliable and suffer from various limitations, especially in the studies of dynamic PPIs. To solve this problem, a method called Bioluminescence Resonance Energy Transfer (BRET) was developed about seventeen years ago. Since then, BRET has evolved into a whole class of methods that can be used to survey virtually any kinds of PPIs. Compared to many traditional methods, BRET is highly sensitive, reliable, easy to perform, and relatively inexpensive. However, most importantly, it can be done in vivo and allows the real-time monitoring of dynamic PPIs with the easily detectable light signal, which is extremely valuable for the PPI functional research. This review will take a comprehensive look at this powerful technique, including its principles, comparisons with other methods, experimental approaches, classifications, applications, early developments, recent progress, and prospects.
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title_short	In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects
url	https://doi.org/10.3390/ijms17101704 https://doaj.org/article/82cd917e24ab498b901c940523113442 http://www.mdpi.com/1422-0067/17/10/1704 https://doaj.org/toc/1422-0067
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In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects

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