Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum

The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T...
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Autor*in:	Mihail Kantor [verfasserIn] Radu Sestras [verfasserIn] Kamal Chowdhury [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Spanisch ; Portugiesisch

Erschienen:	2013

Schlagwörter:	expressão gênica transformação genética malária vacina a partir de plantas gene expression genetic transformation malaria plant-made vaccines

Übergeordnetes Werk:	In: Pesquisa Agropecuária Brasileira - Embrapa Informação Tecnológica, 2004, 48(2013), 1, Seite 73-79
Übergeordnetes Werk:	volume:48 ; year:2013 ; number:1 ; pages:73-79

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ00766673X

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520			\|a The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. Este é o primeiro relato de transformação bem sucedida com a expressão de um antígeno da malária (PfCP-2,9) em plantas transgênicas de tomate da geração T0 e T1.
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allfields	(DE-627)DOAJ00766673X (DE-599)DOAJ2c5668b76c5b4527a924a9857abe0ded DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. Este é o primeiro relato de transformação bem sucedida com a expressão de um antígeno da malária (PfCP-2,9) em plantas transgênicas de tomate da geração T0 e T1. expressão gênica transformação genética malária vacina a partir de plantas gene expression genetic transformation malaria plant-made vaccines Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doaj.org/article/2c5668b76c5b4527a924a9857abe0ded kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010 kostenfrei https://doaj.org/toc/0100-204X Journal toc kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
spelling	(DE-627)DOAJ00766673X (DE-599)DOAJ2c5668b76c5b4527a924a9857abe0ded DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. Este é o primeiro relato de transformação bem sucedida com a expressão de um antígeno da malária (PfCP-2,9) em plantas transgênicas de tomate da geração T0 e T1. expressão gênica transformação genética malária vacina a partir de plantas gene expression genetic transformation malaria plant-made vaccines Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doaj.org/article/2c5668b76c5b4527a924a9857abe0ded kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010 kostenfrei https://doaj.org/toc/0100-204X Journal toc kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
allfields_unstemmed	(DE-627)DOAJ00766673X (DE-599)DOAJ2c5668b76c5b4527a924a9857abe0ded DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. Este é o primeiro relato de transformação bem sucedida com a expressão de um antígeno da malária (PfCP-2,9) em plantas transgênicas de tomate da geração T0 e T1. expressão gênica transformação genética malária vacina a partir de plantas gene expression genetic transformation malaria plant-made vaccines Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doaj.org/article/2c5668b76c5b4527a924a9857abe0ded kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010 kostenfrei https://doaj.org/toc/0100-204X Journal toc kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
allfieldsGer	(DE-627)DOAJ00766673X (DE-599)DOAJ2c5668b76c5b4527a924a9857abe0ded DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. Este é o primeiro relato de transformação bem sucedida com a expressão de um antígeno da malária (PfCP-2,9) em plantas transgênicas de tomate da geração T0 e T1. expressão gênica transformação genética malária vacina a partir de plantas gene expression genetic transformation malaria plant-made vaccines Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doaj.org/article/2c5668b76c5b4527a924a9857abe0ded kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010 kostenfrei https://doaj.org/toc/0100-204X Journal toc kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
allfieldsSound	(DE-627)DOAJ00766673X (DE-599)DOAJ2c5668b76c5b4527a924a9857abe0ded DE-627 ger DE-627 rakwb eng spa por S1-972 Mihail Kantor verfasserin aut Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. Este é o primeiro relato de transformação bem sucedida com a expressão de um antígeno da malária (PfCP-2,9) em plantas transgênicas de tomate da geração T0 e T1. expressão gênica transformação genética malária vacina a partir de plantas gene expression genetic transformation malaria plant-made vaccines Agriculture (General) Radu Sestras verfasserin aut Kamal Chowdhury verfasserin aut In Pesquisa Agropecuária Brasileira Embrapa Informação Tecnológica, 2004 48(2013), 1, Seite 73-79 (DE-627)331758326 (DE-600)2053197-7 16783921 nnns volume:48 year:2013 number:1 pages:73-79 https://doaj.org/article/2c5668b76c5b4527a924a9857abe0ded kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100010 kostenfrei https://doaj.org/toc/0100-204X Journal toc kostenfrei https://doaj.org/toc/1678-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 48 2013 1 73-79
language	English Spanish Portuguese
source	In Pesquisa Agropecuária Brasileira 48(2013), 1, Seite 73-79 volume:48 year:2013 number:1 pages:73-79
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spellingShingle	Mihail Kantor misc S1-972 misc expressão gênica misc transformação genética misc malária misc vacina a partir de plantas misc gene expression misc genetic transformation misc malaria misc plant-made vaccines misc Agriculture (General) Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum
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topic_title	S1-972 Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum expressão gênica transformação genética malária vacina a partir de plantas gene expression genetic transformation malaria plant-made vaccines
topic	misc S1-972 misc expressão gênica misc transformação genética misc malária misc vacina a partir de plantas misc gene expression misc genetic transformation misc malaria misc plant-made vaccines misc Agriculture (General)
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title	Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum
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title_full	Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum
author_sort	Mihail Kantor
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title_sort	transgenic tomato plants expressing the antigen gene pfcp-2.9 of plasmodium falciparum plantas transgênicas de tomate expressando o gene do antígeno pfcp-2.9 de plasmodium falciparum
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title_auth	Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum
abstract	The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. Este é o primeiro relato de transformação bem sucedida com a expressão de um antígeno da malária (PfCP-2,9) em plantas transgênicas de tomate da geração T0 e T1.
abstractGer	The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. Este é o primeiro relato de transformação bem sucedida com a expressão de um antígeno da malária (PfCP-2,9) em plantas transgênicas de tomate da geração T0 e T1.
abstract_unstemmed	The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. Este é o primeiro relato de transformação bem sucedida com a expressão de um antígeno da malária (PfCP-2,9) em plantas transgênicas de tomate da geração T0 e T1.
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title_short	Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum
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Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.<br<O objetivo deste trabalho foi obter plantas transgênicas de tomate que expressem a proteína PfCP-2.9 (uma quimera dos antígenos MSP1 e AMA1 de Plasmodium falciparum). Cotilédones de tomate, cultivar Summers, com sete dias de idade, foram transformados via Agrobacterium tumefaciens. A expressão transgênica nas plantas T0 foi verificada no DNA extraído dos frutos. A análise por PCR foi utilizada para testar a presença do gene de interesse na geração T1. A evidência da expressão do gene no RNA foi constatada por meio da PCR de transcriptase reversa, e a análise "Western blot" confirmou a presença da proteína de interesse nas plantas T1. 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Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum Plantas transgênicas de tomate expressando o gene do antígeno PfCP-2.9 de Plasmodium falciparum

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