Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients

<p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung sec...
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Autor*in:	Salmenkivi Kaisa M [verfasserIn] Soini Ylermi [verfasserIn] Rytilä Paula H [verfasserIn] Peltoniemi Mirva J [verfasserIn] Harju Terttu H [verfasserIn] Board Philip G [verfasserIn] Ruddock Lloyd W [verfasserIn] Kinnula Vuokko L [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2007

Übergeordnetes Werk:	In: Respiratory Research - BMC, 2003, 8(2007), 1, p 48
Übergeordnetes Werk:	volume:8 ; year:2007 ; number:1, p 48

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1465-9921-8-48

Katalog-ID:	DOAJ008327971

Internformat


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520			\|a <p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung secretions, but the mechanisms participating in its homeostasis are partly unclear. Glutathione-S-transferase omega (GSTO) is a recently characterized cysteine containing enzyme with the capability to bind and release GSH <it<in vitro</it<. GSTO has not been investigated in human lung or lung diseases.</p< <p<Methods</p< <p<GSTO1-1 was investigated by immunohistochemistry and Western blot analysis in 72 lung tissue specimens and 40 sputum specimens from non-smokers, smokers and COPD, in bronchoalveolar lavage fluid and in plasma from healthy non-smokers and smokers. It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. The clinical significance of GSTO1-1 and its role in regulating GSH homeostasis in airway secretions, however, needs further investigations.</p<
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allfields	10.1186/1465-9921-8-48 doi (DE-627)DOAJ008327971 (DE-599)DOAJ822ac938cd8b401aadcad88da70863d5 DE-627 ger DE-627 rakwb eng RC705-779 Salmenkivi Kaisa M verfasserin aut Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung secretions, but the mechanisms participating in its homeostasis are partly unclear. Glutathione-S-transferase omega (GSTO) is a recently characterized cysteine containing enzyme with the capability to bind and release GSH <it<in vitro</it<. GSTO has not been investigated in human lung or lung diseases.</p< <p<Methods</p< <p<GSTO1-1 was investigated by immunohistochemistry and Western blot analysis in 72 lung tissue specimens and 40 sputum specimens from non-smokers, smokers and COPD, in bronchoalveolar lavage fluid and in plasma from healthy non-smokers and smokers. It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. The clinical significance of GSTO1-1 and its role in regulating GSH homeostasis in airway secretions, however, needs further investigations.</p< Diseases of the respiratory system Soini Ylermi verfasserin aut Rytilä Paula H verfasserin aut Peltoniemi Mirva J verfasserin aut Harju Terttu H verfasserin aut Board Philip G verfasserin aut Ruddock Lloyd W verfasserin aut Kinnula Vuokko L verfasserin aut In Respiratory Research BMC, 2003 8(2007), 1, p 48 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:8 year:2007 number:1, p 48 https://doi.org/10.1186/1465-9921-8-48 kostenfrei https://doaj.org/article/822ac938cd8b401aadcad88da70863d5 kostenfrei http://respiratory-research.com/content/8/1/48 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2007 1, p 48
spelling	10.1186/1465-9921-8-48 doi (DE-627)DOAJ008327971 (DE-599)DOAJ822ac938cd8b401aadcad88da70863d5 DE-627 ger DE-627 rakwb eng RC705-779 Salmenkivi Kaisa M verfasserin aut Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung secretions, but the mechanisms participating in its homeostasis are partly unclear. Glutathione-S-transferase omega (GSTO) is a recently characterized cysteine containing enzyme with the capability to bind and release GSH <it<in vitro</it<. GSTO has not been investigated in human lung or lung diseases.</p< <p<Methods</p< <p<GSTO1-1 was investigated by immunohistochemistry and Western blot analysis in 72 lung tissue specimens and 40 sputum specimens from non-smokers, smokers and COPD, in bronchoalveolar lavage fluid and in plasma from healthy non-smokers and smokers. It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. The clinical significance of GSTO1-1 and its role in regulating GSH homeostasis in airway secretions, however, needs further investigations.</p< Diseases of the respiratory system Soini Ylermi verfasserin aut Rytilä Paula H verfasserin aut Peltoniemi Mirva J verfasserin aut Harju Terttu H verfasserin aut Board Philip G verfasserin aut Ruddock Lloyd W verfasserin aut Kinnula Vuokko L verfasserin aut In Respiratory Research BMC, 2003 8(2007), 1, p 48 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:8 year:2007 number:1, p 48 https://doi.org/10.1186/1465-9921-8-48 kostenfrei https://doaj.org/article/822ac938cd8b401aadcad88da70863d5 kostenfrei http://respiratory-research.com/content/8/1/48 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2007 1, p 48
allfields_unstemmed	10.1186/1465-9921-8-48 doi (DE-627)DOAJ008327971 (DE-599)DOAJ822ac938cd8b401aadcad88da70863d5 DE-627 ger DE-627 rakwb eng RC705-779 Salmenkivi Kaisa M verfasserin aut Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung secretions, but the mechanisms participating in its homeostasis are partly unclear. Glutathione-S-transferase omega (GSTO) is a recently characterized cysteine containing enzyme with the capability to bind and release GSH <it<in vitro</it<. GSTO has not been investigated in human lung or lung diseases.</p< <p<Methods</p< <p<GSTO1-1 was investigated by immunohistochemistry and Western blot analysis in 72 lung tissue specimens and 40 sputum specimens from non-smokers, smokers and COPD, in bronchoalveolar lavage fluid and in plasma from healthy non-smokers and smokers. It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. The clinical significance of GSTO1-1 and its role in regulating GSH homeostasis in airway secretions, however, needs further investigations.</p< Diseases of the respiratory system Soini Ylermi verfasserin aut Rytilä Paula H verfasserin aut Peltoniemi Mirva J verfasserin aut Harju Terttu H verfasserin aut Board Philip G verfasserin aut Ruddock Lloyd W verfasserin aut Kinnula Vuokko L verfasserin aut In Respiratory Research BMC, 2003 8(2007), 1, p 48 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:8 year:2007 number:1, p 48 https://doi.org/10.1186/1465-9921-8-48 kostenfrei https://doaj.org/article/822ac938cd8b401aadcad88da70863d5 kostenfrei http://respiratory-research.com/content/8/1/48 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2007 1, p 48
allfieldsGer	10.1186/1465-9921-8-48 doi (DE-627)DOAJ008327971 (DE-599)DOAJ822ac938cd8b401aadcad88da70863d5 DE-627 ger DE-627 rakwb eng RC705-779 Salmenkivi Kaisa M verfasserin aut Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung secretions, but the mechanisms participating in its homeostasis are partly unclear. Glutathione-S-transferase omega (GSTO) is a recently characterized cysteine containing enzyme with the capability to bind and release GSH <it<in vitro</it<. GSTO has not been investigated in human lung or lung diseases.</p< <p<Methods</p< <p<GSTO1-1 was investigated by immunohistochemistry and Western blot analysis in 72 lung tissue specimens and 40 sputum specimens from non-smokers, smokers and COPD, in bronchoalveolar lavage fluid and in plasma from healthy non-smokers and smokers. It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. The clinical significance of GSTO1-1 and its role in regulating GSH homeostasis in airway secretions, however, needs further investigations.</p< Diseases of the respiratory system Soini Ylermi verfasserin aut Rytilä Paula H verfasserin aut Peltoniemi Mirva J verfasserin aut Harju Terttu H verfasserin aut Board Philip G verfasserin aut Ruddock Lloyd W verfasserin aut Kinnula Vuokko L verfasserin aut In Respiratory Research BMC, 2003 8(2007), 1, p 48 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:8 year:2007 number:1, p 48 https://doi.org/10.1186/1465-9921-8-48 kostenfrei https://doaj.org/article/822ac938cd8b401aadcad88da70863d5 kostenfrei http://respiratory-research.com/content/8/1/48 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2007 1, p 48
allfieldsSound	10.1186/1465-9921-8-48 doi (DE-627)DOAJ008327971 (DE-599)DOAJ822ac938cd8b401aadcad88da70863d5 DE-627 ger DE-627 rakwb eng RC705-779 Salmenkivi Kaisa M verfasserin aut Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung secretions, but the mechanisms participating in its homeostasis are partly unclear. Glutathione-S-transferase omega (GSTO) is a recently characterized cysteine containing enzyme with the capability to bind and release GSH <it<in vitro</it<. GSTO has not been investigated in human lung or lung diseases.</p< <p<Methods</p< <p<GSTO1-1 was investigated by immunohistochemistry and Western blot analysis in 72 lung tissue specimens and 40 sputum specimens from non-smokers, smokers and COPD, in bronchoalveolar lavage fluid and in plasma from healthy non-smokers and smokers. It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. The clinical significance of GSTO1-1 and its role in regulating GSH homeostasis in airway secretions, however, needs further investigations.</p< Diseases of the respiratory system Soini Ylermi verfasserin aut Rytilä Paula H verfasserin aut Peltoniemi Mirva J verfasserin aut Harju Terttu H verfasserin aut Board Philip G verfasserin aut Ruddock Lloyd W verfasserin aut Kinnula Vuokko L verfasserin aut In Respiratory Research BMC, 2003 8(2007), 1, p 48 (DE-627)326646485 (DE-600)2041675-1 1465993X nnns volume:8 year:2007 number:1, p 48 https://doi.org/10.1186/1465-9921-8-48 kostenfrei https://doaj.org/article/822ac938cd8b401aadcad88da70863d5 kostenfrei http://respiratory-research.com/content/8/1/48 kostenfrei https://doaj.org/toc/1465-9921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2007 1, p 48
language	English
source	In Respiratory Research 8(2007), 1, p 48 volume:8 year:2007 number:1, p 48
sourceStr	In Respiratory Research 8(2007), 1, p 48 volume:8 year:2007 number:1, p 48
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Diseases of the respiratory system
isfreeaccess_bool	true
container_title	Respiratory Research
authorswithroles_txt_mv	Salmenkivi Kaisa M @@aut@@ Soini Ylermi @@aut@@ Rytilä Paula H @@aut@@ Peltoniemi Mirva J @@aut@@ Harju Terttu H @@aut@@ Board Philip G @@aut@@ Ruddock Lloyd W @@aut@@ Kinnula Vuokko L @@aut@@
publishDateDaySort_date	2007-01-01T00:00:00Z
hierarchy_top_id	326646485
id	DOAJ008327971
language_de	englisch
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It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. 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callnumber-first	R - Medicine
author	Salmenkivi Kaisa M
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topic_title	RC705-779 Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients
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title	Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients
ctrlnum	(DE-627)DOAJ008327971 (DE-599)DOAJ822ac938cd8b401aadcad88da70863d5
title_full	Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients
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journal	Respiratory Research
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author_browse	Salmenkivi Kaisa M Soini Ylermi Rytilä Paula H Peltoniemi Mirva J Harju Terttu H Board Philip G Ruddock Lloyd W Kinnula Vuokko L
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title_sort	glutathione s-transferase omega in the lung and sputum supernatants of copd patients
callnumber	RC705-779
title_auth	Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients
abstract	<p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung secretions, but the mechanisms participating in its homeostasis are partly unclear. Glutathione-S-transferase omega (GSTO) is a recently characterized cysteine containing enzyme with the capability to bind and release GSH <it<in vitro</it<. GSTO has not been investigated in human lung or lung diseases.</p< <p<Methods</p< <p<GSTO1-1 was investigated by immunohistochemistry and Western blot analysis in 72 lung tissue specimens and 40 sputum specimens from non-smokers, smokers and COPD, in bronchoalveolar lavage fluid and in plasma from healthy non-smokers and smokers. It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. The clinical significance of GSTO1-1 and its role in regulating GSH homeostasis in airway secretions, however, needs further investigations.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung secretions, but the mechanisms participating in its homeostasis are partly unclear. Glutathione-S-transferase omega (GSTO) is a recently characterized cysteine containing enzyme with the capability to bind and release GSH <it<in vitro</it<. GSTO has not been investigated in human lung or lung diseases.</p< <p<Methods</p< <p<GSTO1-1 was investigated by immunohistochemistry and Western blot analysis in 72 lung tissue specimens and 40 sputum specimens from non-smokers, smokers and COPD, in bronchoalveolar lavage fluid and in plasma from healthy non-smokers and smokers. It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. The clinical significance of GSTO1-1 and its role in regulating GSH homeostasis in airway secretions, however, needs further investigations.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<The major contribution to oxidant related lung damage in COPD is from the oxidant/antioxidant imbalance and possibly impaired antioxidant defence. Glutathione (GSH) is one of the most important antioxidants in human lung and lung secretions, but the mechanisms participating in its homeostasis are partly unclear. Glutathione-S-transferase omega (GSTO) is a recently characterized cysteine containing enzyme with the capability to bind and release GSH <it<in vitro</it<. GSTO has not been investigated in human lung or lung diseases.</p< <p<Methods</p< <p<GSTO1-1 was investigated by immunohistochemistry and Western blot analysis in 72 lung tissue specimens and 40 sputum specimens from non-smokers, smokers and COPD, in bronchoalveolar lavage fluid and in plasma from healthy non-smokers and smokers. It was also examined in human monocytes and bronchial epithelial cells and their culture mediums <it<in vitro</it<.</p< <p<Results</p< <p<GSTO1-1 was mainly expressed in alveolar macrophages, but it was also found in airway and alveolar epithelium and in extracellular fluids including sputum supernatants, bronchoalveolar lavage fluid, plasma and cell culture mediums. The levels of GSTO1-1 were significantly lower in the sputum supernatants (p = 0.023) and lung homogenates (p = 0.003) of COPD patients than in non-smokers.</p< <p<Conclusion</p< <p<GSTO1-1 is abundant in the alveolar macrophages, but it is also present in extracellular fluids and in airway secretions, the levels being decreased in COPD. The clinical significance of GSTO1-1 and its role in regulating GSH homeostasis in airway secretions, however, needs further investigations.</p<
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title_short	Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients
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Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients

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