Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination

The generation and application of human stem-cell-derived functional neural circuits promises novel insights into neurodegenerative diseases. These networks are often studied using stem-cell derived random neural networks in vitro, with electrical stimulation and recording using multielectrode array...
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Autor*in:	Felix Schmieder [verfasserIn] Simon D. Klapper [verfasserIn] Nektarios Koukourakis [verfasserIn] Volker Busskamp [verfasserIn] Jürgen W. Czarske [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	ferroelectric liquid crystal computer-generated hologram optogenetics

Übergeordnetes Werk:	In: Applied Sciences - MDPI AG, 2012, 8(2018), 7, p 1180
Übergeordnetes Werk:	volume:8 ; year:2018 ; number:7, p 1180

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/app8071180

Katalog-ID:	DOAJ00863372X

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authorswithroles_txt_mv	Felix Schmieder @@aut@@ Simon D. Klapper @@aut@@ Nektarios Koukourakis @@aut@@ Volker Busskamp @@aut@@ Jürgen W. Czarske @@aut@@
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callnumber-first	T - Technology
author	Felix Schmieder
spellingShingle	Felix Schmieder misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc ferroelectric liquid crystal misc computer-generated hologram misc optogenetics misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination
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topic_title	TA1-2040 QH301-705.5 QC1-999 QD1-999 Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination ferroelectric liquid crystal computer-generated hologram optogenetics
topic	misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc ferroelectric liquid crystal misc computer-generated hologram misc optogenetics misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry
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title	Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination
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title_sort	optogenetic stimulation of human neural networks using fast ferroelectric spatial light modulator—based holographic illumination
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title_auth	Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination
abstract	The generation and application of human stem-cell-derived functional neural circuits promises novel insights into neurodegenerative diseases. These networks are often studied using stem-cell derived random neural networks in vitro, with electrical stimulation and recording using multielectrode arrays. However, the impulse response function of networks is best obtained with spatiotemporally well-defined stimuli, which electrical stimulation does not provide. Optogenetics allows for the functional control of genetically altered cells with light stimuli at high spatiotemporal resolution. Current optogenetic investigations of neural networks are often conducted using full field illumination, potentially masking important functional information. This can be avoided using holographically shaped illumination. In this article, we present a digital holographic illumination setup with a spatial resolution of about 8 µm, which suffices for the stimulation of single neurons, and offers a temporal resolution of less than 0.6 ms. With this setup, we present preliminary single-cell stimulation recording of stem-cell derived induced human neurons in a random neural network. This will offer the opportunity for further studies on connectivity in such networks.
abstractGer	The generation and application of human stem-cell-derived functional neural circuits promises novel insights into neurodegenerative diseases. These networks are often studied using stem-cell derived random neural networks in vitro, with electrical stimulation and recording using multielectrode arrays. However, the impulse response function of networks is best obtained with spatiotemporally well-defined stimuli, which electrical stimulation does not provide. Optogenetics allows for the functional control of genetically altered cells with light stimuli at high spatiotemporal resolution. Current optogenetic investigations of neural networks are often conducted using full field illumination, potentially masking important functional information. This can be avoided using holographically shaped illumination. In this article, we present a digital holographic illumination setup with a spatial resolution of about 8 µm, which suffices for the stimulation of single neurons, and offers a temporal resolution of less than 0.6 ms. With this setup, we present preliminary single-cell stimulation recording of stem-cell derived induced human neurons in a random neural network. This will offer the opportunity for further studies on connectivity in such networks.
abstract_unstemmed	The generation and application of human stem-cell-derived functional neural circuits promises novel insights into neurodegenerative diseases. These networks are often studied using stem-cell derived random neural networks in vitro, with electrical stimulation and recording using multielectrode arrays. However, the impulse response function of networks is best obtained with spatiotemporally well-defined stimuli, which electrical stimulation does not provide. Optogenetics allows for the functional control of genetically altered cells with light stimuli at high spatiotemporal resolution. Current optogenetic investigations of neural networks are often conducted using full field illumination, potentially masking important functional information. This can be avoided using holographically shaped illumination. In this article, we present a digital holographic illumination setup with a spatial resolution of about 8 µm, which suffices for the stimulation of single neurons, and offers a temporal resolution of less than 0.6 ms. With this setup, we present preliminary single-cell stimulation recording of stem-cell derived induced human neurons in a random neural network. This will offer the opportunity for further studies on connectivity in such networks.
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title_short	Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination
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Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination

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